g12 slhl humanbio
TRANSCRIPT
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Homework
Describe on one piece of paper how electropheresis works
MUST: isoelectric pointZwitterions
SHOULD: Buffer effect, PAGECOULD: Effect of bonding on pH
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Task One
• Protein Structure– http://www.youtube.com/watch?v=lijQ3a8yUYQ
– http://www.youtube.com/watch?v=iaHHgEoa2c8&NR=1
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What are Proteins For ?
• Enzymes• Structure to; hair, nails• Energy• Hormones, e.g. FSH – menstrual cycle
stimulant……
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Chromatography
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• http://www.chemie.uni-regensburg.de/Organische_Chemie/Didaktik/Keusch/D-Fehling-e.htm
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Carbohydrates
• Monosaccharides - structure
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Fructose isomers
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Glucose Isomers
• http://www.biotopics.co.uk/JmolApplet/alphabetajglucose2.html
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Condensation Rxns of the Monosaccharides
• Formation of
– Starch
– Glycogen
– Cellulose
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What do Carbohydrates do for us ?
• Energy source (glucose), energy reserves (glycogen) and precursors for other biologically important molecules.
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Humans can Digest Starch NOT Cellulose
• Both are polymers of glucose units. • Starch has two forms: amylose, which is a
straight-chain polymer (α 1,4 linkage), and ‑amylopectin, which is a branched structure with both α 1,4 and α 1,6 linkages. ‑ ‑
• Cellulose has a β 1,4 linkage; this can be ‑hydrolysed by the enzyme cellulase, which is absent in most animals, including mammals.
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Dietary Fibre
• Dietary fibre may be helpful in the prevention of conditions such as diverticulosis, irritable bowel syndrome, constipation, obesity, Crohn’s disease, hemorrhoids and diabetes mellitus.
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Fehlings’ Test
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Lipids
• triglycerides (fats and oils),– http://tinyurl.com/yda73qr
• phospholipids (lecithin)
• steroids (cholesterol )
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HDLand LDL cholesterol
• http://www.americanheart.org/presenter.jhtml?identifier=180
• LDL =
• HDL =
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Saturated and unsaturatedfatty acids.
• Naturally occurring fatty acids have a mixture of unsaturated, saturated and polyunsaturated fatty acids.
• http://en.wikipedia.org/wiki/Fatty_acid
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Omega-6 and omega-3 fatty acids
• Omega 6
• Omega 3
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Iodine number
• The number of moles of I2 reacting with one mole of fat/oil indicates the number of double bonds present in the fat/oil molecule.
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Enzyme-catalysedhydrolysis of triglycerides
• Ester hydrolysis to carboxylic acid + alcohol
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Energy content
• Fats v Carbohydrates
• Carbs – quick rapid absorption
• Fats – slow release of energy over time, roughly 2x the amount of energy contained in a carb
• http://www.merck.com/mmhe/sec12/ch152/ch152b.html
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Roles of lipids
• energy storage• insulation and protection of organs• steroid hormones• structural component of cell membrane• omega-3 poly-unsaturated fatty acids reduce• the risk of heart disease• poly-unsaturated fats may lower levels of LDL
cholesterol
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Negative Effects
• increased risk of heart disease from elevated levels of LDL cholesterol and trans fatty acids;
• the major source of LDL cholesterol is saturated fats, in particular lauric (C12), myristic (C14) and palmitic (C16) acids
• obesity.
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Micronutrients
• Micronutrients are substances required in very small amounts (mg or μg) and that mainly function as a co-factor of enzymes (<0.005% body weight).
• Examples include vitamins and trace minerals (Fe, Cu, F, Zn, I, Se, Mn, Mo, Cr, Co and B).
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Macronutrients
• These are chemical substances that are required in relatively large amounts (>0.005% body weight).
• Examples include proteins, fats, carbohydrates and minerals (Na, Mg, K, Ca, P, S and Cl).
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(vitamin A), calciferol (vitamin D) andascorbic acid (vitamin C).
• Structural comparison
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Deduce whether a vitamin is water- orfat-soluble from its structure.
• water-soluble—vitamins B and C
• fat-soluble—vitamins A, D, E and K.
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Homework
• Discuss the causes and effects of nutrient deficiencies in different countries and suggest solutions
• (word doc)
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Hormones
• Hormones are miccheal messengers. They are crtdeese directly into the blood by endocrine glands. Examples lucenid ADH, aldosterone, estrogen, progesterone and testosterone, insulin, epinephrine (adrenaline) and thyroxine.
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The structures of cholesteroland the sex hormones
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The mode of action of oralcontraceptives.
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The use and abuse of steroids.
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Higher Level
• Enzymes• Nucleic Acids• Respiration
• In GREAT chemical detail
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Enzymes
• enzymes are proteins; activity depends on tertiary and quaternary structure; and the specificity of enzyme action.
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Compare inorganic catalysts andbiological catalysts (enzymes).
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The relationship betweensubstrate concentration and enzyme
activity.
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Vmax and the value of theMichaelis constant (Km) by graphical
means
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Mechanism of Enzyme Action
• enzyme substrate complex, active site and induced fit model.
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Competitive inhibition andnon-competitive inhibition
• http://en.wikipedia.org/wiki/File:Comp_inhib.svg
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Enzyme Activity
• Effect of :-– Heavy metal ions
– Temperature
– pH
– http://www.elmhurst.edu/~chm/vchembook/573inhibit.html
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Nucleic Acids
• Nucleic acids are polymers made up of nucleotides.• A nucleotide contains a phosphate group, a pentose sugar
and an organic nitrogenous base.• Students should recognize, but do not need to recall, the
structures of the five bases: adenine (A), cytosine (C), guanine (G), thymine (T) and uracil (U).
• Nucleic acids are joined by covalent bonds between the phosphate of one nucleotide and the sugar of the next, resulting in a backbone with a repeating pattern of sugar–phosphate–sugar–phosphate. Nitrogenous bases are attached to the sugar of the backbone.
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RNA and DNA
• RNA has ribose as its pentose sugar; DNA has deoxyribose. Deoxyribose lacks an oxygen atom on C2. RNA has uracil instead of thymine as its base.
• RNA is a single strand nucleic acid; DNA is a ‑doublestrand nucleic acid.
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DNA double helix
• The structure has two nucleic acid strands that spiral around an axis.
• Describe the hydrogen bonding between specific pairs of nucleotide bases
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The role of DNA
• repository of genetic information.• explain its role in protein synthesis……….
• DNA is the genetic material that an individual inherits from its parents. It directs mRNA synthesis (transcription) and, through mRNA, directs protein synthesis (translation) using a triplet code.
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DNA Profiling
• Uses in forensic and paternity cases.
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Respiration
• Aerobic and anaerobic respiration • In aerobic respiration, glucose is converted into
pyruvate, which, in the presence of oxygen, changes to carbon dioxide and water. Overall, glucose undergoes oxidation and oxygen undergoes reduction. In anaerobic respiration, pyruvate is converted to lactate in human beings whereas yeast converts pyruvate to ethanol and carbon dioxide.
• Redox equations should be used.
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The role of Cu
• Cytochromes
• Hemoglobin