Nanoscopy at the interfaceFrom Materials to Ice

Roderick Tas

4TU-workshopUtrecht, October 28th 2019

Voets LabSelf-Organizing Soft Matter

Nanoscale assemblies in chemistry and soft matter

A) Q. Chen et al., Nature, 2011.B) M.E. Leunissen et al., Nature, 2005.

E) C.M.A. Leenders et al., Chem. Comm., 2013.F) M. Beuwer et al., Pol. Chem., 2016.

Assembly of colloids and colloidal crystals Assembly of supramolecular molecules

A B

C D

E

F

C) W.T.M. Irvine et al., Nat. Mater., 2012.D) A. Yethiraj et al., Nature, 2003.

Soft Matter microscopy

Zeiss

Soft Materials

In Situ imagingLabeling specificityDynamic information

Super resolution microscopy

20 nm

Electron Microscopy

Flavours of super-resolution microscopy

Ni et al., 2017 JBioPhotonics

~100nm ~50nm ~10nmLateral resolution

~s ~s ~minTemporal resolution

Single Molecule Localization Microscopy

(SMLM)

Single molecule localization microscopy

widefield image acquisition reconstructionlocalizations

midpoints200nm

Lessons from biology

Periodic rings in neuronal neuritesXu et al., 2015 Science

Microtubule orientations dictate selective transport in neuronsTas et al., 2018 Neuron

Spatially resolved single-cell RNA profilingMoffit et al., 2018 Science

Nanoscopy in material sciences

Albertazzi et al., 2014 Science Conley et al., 2016 CSA

2D micelles characterizationsupramolecular polymers Particle composition and density

Qiu et al., 2016 Science

• iPAINT: a versatile SMLM tool to study soft matter• Colloidal dispersion in fluid-fluid interfaces• Complex supramolecular polymers

• Resolving antifreeze proteins at the ice-water interface to study their mechanisms

Outline: Applications of SMLM to understand soft matter

iPAINT

Aloi et al., 2016, Nanoscale

Transient non-covalent adsorption of fluorophores at interfaces

iPAINT: Colloidal dispersions and interfaces

Aloi, IKV, RSC Nanoscale 2016

iPAINT: Colloidal dispersions and interfaces

oil

water

iPAINT of a liquid-liquid interface

Aloi, IKV, RSC Nanoscale 2016

iPAINT: Colloidal dispersions in a fluid interface

Lowers interfacial tension

Reversibly adsorbed

Lowers interfacial tension Forms steric barrier

Irreversibly adsorbed

Surfactants Particles

iPAINT: Colloidal dispersions in a fluid interface

oil

water

water

oil

Oil phase

Water phase

γso

γow

γsw

θow

Θ > 90°

Θ < 90°

E. C. Giakoumatos et al., In preparation.

iPAINT: Colloidal dispersions in a fluid interface

Scale bar 500 nmE. C. Giakoumatos et al., In preparation.

iPAINT: In situ polymer assembly (in organic solvent)

B. Adelizzi,* A. Aloi* et al., ACS Nano, 2018.

WaterProbe exchange

Organic SolventNo exchange

iPAINT: In situ polymer assembly (in organic solvent)

B. Adelizzi,* A. Aloi* et al., ACS Nano, 2018.

iPAINTing soft matter

Interfaces Supramolecular systemsColloidal dispersions

1 mm

Aloi, IKV, RSC Nanoscale 2016

Summary: SMLM to understand soft matter

Complex supramolecular polymersColloidal dispersions in fluid-fluid interfaces

Ice growth inhibition by Antifreeze proteins

Acknowledgements

Prof. Dr. Ilja VoetsDr. Antonio Aloi iPAINTEmma Giakoumatos Colloidal dispersionsRomà Suris VallsChristian Sproncken

VoetslabLaboratory of Self-Organizing

Soft Matter