from images to answers portfolio de productos de media cybernetics olympus latin america q2 2006
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FROM IMAGES TO ANSWERS
Portfolio de Productos de Media Cybernetics Olympus Latin America Q2 2006
New Releases for 2006
•AutoQuant X
•InVivo/InVitro 3
•Image-Pro Plus 6 (Slightly Higher Price but Lots More Stuff)
•Scope-Pro 6 (Piezo Support and More…)
•Image-Pro Analyzer 6 (Single Seat, 3-Seat)
•Image-Pro Express 6 (Lower Price; More Features)•IPE 3DS
•IPE-MC
•Scope-Pro Express 6 (Z and Shutter Control)
•Vertical Applications:•IQ-Otolith & IPP-Otolith Macro
•EDF Analyzer
•IQbase 2
New Releases
InVivo 3
Top Features in InVivo 3
•1-Button Data Set Export to Image-Pro Plus 6.0
•Yes
•Finally
•Windows XP x64 Support
•Large memory address handling
•64-bit camera drivers from QImaging and Hamamatsu
•Variable Rate Time Lapse Acquisition
•Nyquist Calculation for Z-Stack Acquisition
•Multi-Camera Support
•Support for Up To Four Concurrent Cameras
•True Simultaneous Acquisition from Two Monochrome Photometrics or QImaging Cameras
•Updated Driver Support for Other Popular Cameras
Top Features in InVivo 3
•FRET Acquisition
•Live Calibrated Ratio Display
Top Features in InVivo 3, part deux
•Stage Navigation Upgrades
•Cell Chambers and Multi-Well Plate templates
Top Features in InVivo 3, part troix
•New Microscope Control Dialog Illustrations for Leica Stands
•Support for DM6000, 5000, 4000 & DMI6000, 5000, 4000
InVivo Family Software Series
InVivo Live Cell Imaging Solution for Calcium Ratios, FRET and Other Dynamic Processes
ExpIO Vital Systems Control and Measurement
Image Renamed “InVitro” at Lower Price
Capture Discontinued
• Photometrics Family of Monochrome Cameras (including MAC, but NOT AS FAST! [no circular buffer support])
• QImaging Family of Cameras (including MAC)• Some Hamamatsu Cameras (not including Mac)• Some Stanford Photonics Cameras
Digital Camera Support
InVivo Acquisition
Camera Control
Fully Automated Microscope Control
Multi Color Acquisition
Time Lapse, Burst Acquisition
3D, z-series, Burst 3D with piezo
Tiling, Mosaic, Slide and Well Scanning
FRET Acquisition
Optical Splitter Acquisition
Multi Dimensional Acquisition
InVivo Analysis
Live Ratio and Ca++ Imaging
Intensity Calibration (Including Grynkewicz)
Vital Systems Control and Measurement
Signals and Systems control
Continuous Wavelength Imaging Devices
Live Cell Imaging
Pre-Configured Systems
• Automated Microscopes
• Digital Cameras
• Stage Automation
• Vital Systems
Live Cell Imaging
Application Solutions with InVivo Bundles
Multi Color Time Lapse Acquisition
Live Intensity and Ratio Measurements with Fast Acquisition using InVivo
Advanced Ratiometric and Intensity-based Tracking in Image-Pro Plus
Time Lapse, Burst Acquisition and Advanced Tracking
High Speed Acquisition for Small Region(s)
Track Single or Multiple Moving Particles
3D Acquisition, Deconvolution and Measurement
Multicolor Z and Burst Z Acquisition
Multi Color Deconvolution 2D & 3D Blind
Advanced 3D Analysis and Measurements
Multidimensional Acquisition and Analysis
Count Size
Extended Depth of Field
Signals and Actions Acquisition and Analysis
www.mediacy.comSilver Spring, MD (Headquarters)
Offices in:
Boston, MA, Pittsburgh, PA & San Diego, CA
Leiden, The Netherlands
Göttingen, Germany
Wokingham, United Kingdom
Singapore, Republic of Singapore
Tokyo, Japan
Shanghai, China
New Releases
Image-Pro Plus 6
Top New Features in Image-Pro 6
•Live Image Scaling for Low-Light Applications
•Live Data Collection and Charting
•XP x64 Support to Open Larger Image Sets
•Open GL 3D Viewer
•Direct Import of QED Files (File, Open)
•User-Defined Equation Editor in Data Collector
•Dynamically Linked Graphic Display in Data Collector
•Embedded I/O Control for Serial and Parallel Devices
•ICC Color Profiles
New Open GL 3D Viewer
More File Support
New Data Collector Tools
New Data Input/Output Tool
New Color Management Tool
Image-Pro 6 Enhancements
•Image-Pro Plus 6 Feature Enhancements:
•Updated Capture Dialog
•Scalable Calibration Markers
•User-Specific Menus
•“Dark Mode” for Low Light Laboratory Environments
•Automatic Measurement of >100,000 Objects (Memory-Dependent)
•Intelligent Macro Editor (not Intelli-Sense, but something much like it ;) )
•3D Convolution Filters
•More Extensive File Support (FV1000, JPEG2000, etc.)
•Export to Excel or Origin
•Generate Movies from Surface Plot
•6-Month License for IQbase 2 (Optional Permanent License at Extra Cost)
Updated Capture Dialog
New Scalable Calibration
New Menu Editor
New Dark Mode
New 3D Convolution Filters
Top New Features in Scope-Pro 6
•3-Point Predictive Focus for Wavy Samples
•Support for Simultaneous Stepper and Piezo Drives (X, Y, and Z)
•Calibrated Continuous Zoom Support for Olympus SZ-X
•Objective Paracentricity (X,Y) Offsets
•Analog/TTL Support for Shutters, Filter Wheels, AOTF’s and Monochromators
•Nyquist Calculator for Z-Axis Acquisition
•Support for the Olympus ZDC & Nikon Perfect Focus
Image-Pro Family Modules
•Automate Motorized Microscopes and Other Acquisition Devices
• A/D & D/A Acquisition & Support for External Devices
•SharpStack Plus includes: 3D Blind, 2D Blind deconvolution algorithms plus No Neighbor, Nearest Neighbor and Inverse Filter algorithms
•Turbo Scan•High Speed Tiling
•Back as a Plug-In by Popular Request
• QImaging* (Excellent)• Photometrics* (Excellent)• Leica DFC* (Excellent)• Olympus DP50 / DP70 / DP30 (Excellent)• Nikon DXM1200/F DS5 (Good)• Hamamatsu (Good, 3rd Party Driver)• Cooke PCO (Good, 3rd Party Driver)• Diagnostic Instruments (Fair, 3rd Party Driver)• Lumenera (Fair, 3rd Party Driver)
Camera Support for Image-Pro Family
* Image-Pro 6.X Support
www.mediacy.comSilver Spring, MD (Headquarters)
Offices in:
Boston, MA, Pittsburgh, PA & San Diego, CA
Leiden, The Netherlands
Göttingen, Germany
Wokingham, United Kingdom
Singapore, Republic of Singapore
Tokyo, Japan
Shanghai, China
New Releases
IQbase 2
Top New Features of IQbase 2
•IQbase 2 Features:
•Capture Module (Optional extra Cost)
•Support for Active Directory – Use Windows Identities for log-in and Security Levels
•Export Images and Data to PowerPoint
•Apply Measurements to Archived Images
•Compare Images Side-by-Side using Lightbox Tool
•Use 3D Scatterplots to Mine Data in Three Dimensions
•Speed Optimized for Analysis, Searching, Opening and Displaying
•New Manual Measurement Tools
IQbase 2 Powering Image Informatics
•Manages Images and Data•Drives Discovery •Facilitates Collaboration•Easy to Use•Works the Way You Do•Grows with Your Organization (Scalable)
IQstudioAnalytical Imaging Development Kit
Rapid Development of Image Analysis Applications
•Develop Applications Using our Tools•Add Image Analysis to Existing Products•Standardize Image Analysis within the Organization•Distribute Custom Applications with Run-Time Licensing
Deconvolution, 3D Rendering & Measurement
Deblurring vs Deconvolution
Auto-Quant® Algorithms are Used in Sharpstack
The No Neighbor & Nearest Neighbor Algorithms are Deblurring Tools (Intensities are Removed)
The Inverse Filter Algorithm is a Haze Restoration Tool (Intensities are Re-Assigned)
The Optional 2D Blind and 3D Blind Tools are Deconvolution Algorithms (Intensities are Re-Assigned)
The Sharpstack Algorithms are not Appropriate for Confocal Images Because They Use Widefield PSFs
Deblurring vs Deconvolution
AutoQuant X
AQI X Advantage
• Sequence detection– Multi time point– Multi channel
• Multi-Processing, Multi-Threaded• Best in Deconvolution
– Only Blind Algorithm Commercially Available
– Spherical Aberration Detection and Correction
Sequence Detection
• Loads Complex File Sequences• Dynamically Changes to Accommodate Naming of Current File
Multi Time Point
• Load 3D Time Series Data• Visualize, Deconvolve and Analyze as a
Single Dataset
Multi Channel
• Load any Number of Channels• Change Tints & Thresholds in Real-Time
Multi-Processing, Multi-Threading
• Each Operation Runs in its Own Thread• Deconvolve, Visualize a Dataset and Queue Another
Dataset at the Same Time• Run Two Deconvolutions Simultaneously• Status Display is a Dashboard for Activity• Takes Advantage of Dual Processor Computers
Blind Deconvolution
• Blind Deconvolution Yields the Best Results with the Least Effort
• No Need to Acquire or Maintain a Library of Point Spread Functions
• Adaptive PSF Changes with the Microscope: – Aberrations, Thermal Expansion, Noise,
Alignment, and Specimen are Corrected• Maximum Likelihood Expectation Models
Noise Instead of Smoothing it
Hierarchy of Algorithms
• 3D Blind Deconvolution – Premier• 2D Blind Deconvolution – Live cell• 3D Non-Blind - Faster for Derived
PSFs• 3D Non-Blind (Gold’s) – Fast but
Noisy• 3D Inverse Filter, Nearest/No
Neighbor – Quick and Dirty
Spherical Aberration
• Common Problem with RI Mismatches or Thick Samples
• Detect Using Iterative MLE or Calibrate• Pre-Bias PSF Improves with Blind
Deconvolution• Improves Signal Due to Smearing
Deconvolution of Widefield and Confocal Images
Primer on The Growing Role of Deconvolution…
Why Use Deconvolution?
To Improve Signal to Noise Ratio
What is Deconvolution?
Widefield – Single Channel
Multiple Wavelengths - WidefieldMultiple Wavelengths - Widefield
Confocal MicroscopyConfocal Microscopy
2D Blind Deconvolution2D Blind Deconvolution
2D Blind Deconvolution – TIRF2D Blind Deconvolution – TIRF
When to Use Deconvolution?
In Widefield Fluorescent Microscopy– Improve contrast of 2D and 3D Images– Improve Resolution in Three Dimensions– Improve 3D Rendering and Visualization
When to Use Deconvolution?
In Confocal Microscopy– Improve Signal to Noise Ratios– Increase Dynamic Range– Improve Image Grayscale
Resolution– Increase Axial Resolution
When to Use Deconvolution?
Documentation– Images and Projections will Show
More Detail and Greater Contrast– Improvements can be Striking
When to Use Deconvolution?
Analysis– Deconvolution Retains Quantitative
Accuracy– Improves Contrast Facilitating Analysis
• Counting • Tracking• Area Measurements• Intensity Measurements
Why Does This Work?
The Microscope is a Well-Understood Optical System
The Behavior of Light is Well-Modeled
Persistence
Why this works (1/5)Why this works (1/5)
The Point Spread of a Microscope
• Every Microscope-Sample Combination has a Different Point Spread Function
• Every Element in the Optical Path Affects the Point Spread Function:
– Scope– Objective– Cover slip– Sample
The Point Spread of a Microscope
Compare a widefield PSF to a Confocal PSF
Looking Forward to Deconvolution
YFP Labeled Motor Neurons (Mouse)
Laura Baylor, Harvard UniversityLaboratory of Dr. Jeff Lichtman
Emissive WavelengthEmissive Wavelength
Numerical ApertureNumerical Aperture
Spherical AberrationSpherical Aberration
Non-Blind deconvolution estimates the original object from the measured PSF and the collected data (2D or
3D image).
Non-Blind Deconvolution
Blind deconvolution separates the original object and the point spread from the collected data (2D or 3D
image).
Blind deconvolution
3D User Interface
3D User Tools
•Pointer to Toggle between Measurements & Volume Display
•Pan Tool to Adjust 3D Volume Position
•Home Position
•Set New Home Position
•Change Perspective (View)
•Display Box Around Volume
•Change View Y,Z or Z,X or Y,X
•Use Control Click for LR or UD, Click for UD, Alt Click to Toggle Between Pan and Pointer
3D User Options
3D Rendering with 3D Filters
3D Volume Measurements
3D Volume Measurements Over Time
3D Branch / End Point Measurements
www.mediacy.comSilver Spring, MD (Headquarters)
Worldwide Offices in:
Boston, Pittsburgh & San Diego
Leiden, The Netherlands
Goettingen, Germany
Wokingham, United Kingdom
Singapore, Republic of Singapore
Tokyo, Japan
Shanghai, China