fred martin micrograph portfolio
DESCRIPTION
Sample of images taken for MMP program using Zeiss Axioimager, Nikon, Olympus AX41, LeicaTRANSCRIPT
PROJECT PORTFOLIO
FRED MARTIN12/08/2009 Dr. GiorgiBIOSC 2
Invt4_10x_Com
positeRG
B_002
.jpg
20091207 /Nikon2 /10x ; DIC: 73ms/ 1,503/ 0.95/ 4,095 R: 97ms/ 79/ 0.63/ 4,095 G: 200ms/ 239/ 0.95/4,095 B:500ms/223/0.77/4,095
SP
EC
IME
N (A
1)
NIKON, FLUORESENCE, MOLECULAR PROBES SLIDE: TISSUE
invt4_a1_Com
posite_RG
_DapiLU
T_75.jpg
20091123 /Nikon2 /20x ; DIC: 20ms/ 79/ 0.94/ 3,310 R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830
SP
EC
IME
N (A
2)
NIKON, FLUORESENCE, MOLECULAR PROBES SLIDE: TISSUE
FluoCells prepared slide #2: BPAE bovine pulmonary artery endothelial cells
Image A
- 20091105: Zeiss #1 20x
ZEISS, FLUORESENCE, MOLECULAR PROBES SLIDE: CELLS
FluoCells® prepared slide #1: BPAEC, Zeiss 63x
20091016_INV
G1_A
RE
A3_C
ompo
siteAdj_jpg.jpg
ZEISS, FLUORESENCE, MOLECULAR PROBES SLIDE: CELLS
NIKON, FLUORESENCE, MOLECULAR PROBES SLIDE: CELLS
FM
_INV
T1_A
1_C
om
positeAdjR
GB
_xDIC
BP
AE
CE
LLS IN
VIT
RO
GE
N #1
20x_LilyAnther_F
luro_RG
B.jpg
20091123 /Nikon2 /20x ; R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830
SP
EC
IME
N (B
)
NIKON, FLUORESENCE
FM
_lycopodiumC
aptured_F
luoroRG
BnD
IC_20x_2.jpg
20091123 /Nikon2 /20x ; DIC: 20ms/ 79/ 0.94/ 3,310 R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830
SP
EC
IME
N (C
)
NIKON, FLUORESENCE + DIC
SP
EC
IME
N (F
)T
ypha_10x_Com
posite_001_RG
B_A
dj
20091207 /Nikon2 /10x ; DIC: 73ms/ 1,503/ 0.95/ 4,095 R: 97ms/ 79/ 0.63/ 4,095 G: 200ms/ 239/ 0.95/4,095 B:500ms/223/0.77/4,095
COMPOSITE CHANNEL: RED
CHANNEL: GREEN CHANNEL: BLUE
NIKON, FLUORESCENSE (3 CHANNELS + OVERLAY)
Pollen Grain: Abutilon x hybridium @ 20x
EM Channel: Green Exp: 96.7ms Gain:7.1 Offset: -876
EM Channel: Red Exp: 96.7ms Gain:7.1 Offset: -876EM Channel: (BF) Exp: 16.7ms Gain: 4.5 Offset: -161
Composite
OLYMPUS, OWN SPECIMEN, FLUORO OVERLAY + 3 CHANNELS
fm_
_pseudocolor_lycopod_20x_Com
positeRG
Bn
DIC
.jpgS
PE
CIM
EN
(D)
20091207 /Nikon2 /20x ; “R”: 13ms/ 159/ 0.51/ 2,830 “G”: 6.6ms/ 159/ 0.51/ 2,830 “B”: 12.0ms/ 159/ 0.51/ 2,830
NIKON, BRIGHTFIELD (3 CHANNEL PSEUDO-COLORIZATION)
Multichannel _S
hrim
p_Pseudocolor2_R
GB
.jpgS
PE
CIM
EN
(E)
20091118 /Nikon4 /20x ; R: --ms/ --/ --/ -- G: --ms/ --/ --/ -- --B: ms/ --/ --/ -- Note: metadata not logged
NIKON, BRIGHTFIELD (3 CHANNEL PSEUDO-COLORIZATION)
Cilia
PSCCE Cells
Basal Lamina
Pseudo Stratified Ciliated Columnar Epithelial Cross section: area 3 image
Cilia
Basal Lamina
Nucleus
Cilia
(Apparent Stratification)Exp. 500.7ms
Hist: 79/252
Gamma: 0.99
Auto_exp: OFF
Gain: 3.1x
2009.10.01Leica #2 60x (dry)
Nucleolus {
}
LEICA, BRIGHTFIELD
48BitDepth:20 / 255Histogram:OnAuto_Exp?0.97Gamma:
2009-10-07DateImage:53 %Brightness:2.2 xGain:1.0 sExposure:
PSCCE CellsCell Membrane
Cilia(Apparent Stratification)
Nuclei
Basal Lamina
LEICA, BRIGHTFIELD
ZEISS, DIC
FM
_INV
T4_A
1_D
IC-0005.JP
G
20091209 /ZEISS 1 /20x ; DIC : EXP 69ms/ CT -0.50 / GAMMA 1.0 / BRT 1.0
- Interphase
- Metaphase
- Anaphase- Telophase
- Prophase
RESULTS: DIGITAL COUNT DETAIL – FIELD OF VIEW A: Onion Root Tip
Meta Data Scope:
Olympus 3 (A)
10x, rheo: full
Iris ~ 30% 1.1 ms2.8 gain-1,860 offset
Note: Manual countdone at 20x on Olympus3
OLYMPUS, MORPHOMETRY