fraction collector f9-c and fraction collector f9-r

Fraction collectorF9-C and F9-ROperating Instructions

Original instructions

cytiva.com

http://cytiva.com

Table of Contents

1 Introduction ........................................................................................................ 41.1 About this manual ......................................................................................................................................... 51.2 Important user information ....................................................................................................................... 6

2 Safety instructions ............................................................................................. 82.1 Safety precautions ........................................................................................................................................ 92.2 Symbols and abbreviations ........................................................................................................................ 152.3 Emergency procedures ............................................................................................................................... 17

3 Fraction collector overview ............................................................................... 193.1 Fraction collector F9-C ............................................................................................................................... 20

3.1.1 Function ............................................................................................................................................................ 213.1.2 Fraction collector F9-C illustrations ..................................................................................................... 223.1.3 Cassettes, Cassette tray and racks ...................................................................................................... 263.1.4 Connect tubing to the ÄKTA pure instrument .................................................................................. 30

3.2 Fraction collector F9-R ............................................................................................................................... 313.2.1 Function ............................................................................................................................................................ 323.2.2 Fraction collector F9-R illustrations ..................................................................................................... 333.2.3 Tubes ................................................................................................................................................................. 363.2.4 Connect tubing to ÄKTA pure or ÄKTA avant ................................................................................... 38

4 Installation .......................................................................................................... 394.1 Room and site requirements ..................................................................................................................... 40

4.1.1 Delivery and storage ................................................................................................................................... 414.1.2 Placement and space requirements .................................................................................................... 424.1.3 Site requirements ......................................................................................................................................... 45

4.2 Connecting two fraction collectors to ÄKTA pure ........................................................................... 464.2.1 Power and communication ...................................................................................................................... 474.2.2 Connect tubing .............................................................................................................................................. 53

4.3 Prepare waste tubing for Fraction collector F9-C ............................................................................ 554.4 Set the delay volume ..................................................................................................................................... 574.5 Performance test ........................................................................................................................................... 59

5 Operation ............................................................................................................ 605.1 Prepare Fraction collector F9-C .............................................................................................................. 615.2 Fraction collector F9-R ............................................................................................................................... 68

6 Maintenance ....................................................................................................... 756.1 Clean Fraction collector F9-C ................................................................................................................... 766.2 Fraction collector F9-R ............................................................................................................................... 78

7 Reference information ....................................................................................... 797.1 Recycling information .................................................................................................................................. 817.2 Regulatory information ............................................................................................................................... 82

7.2.1 Contact information .................................................................................................................................... 837.2.2 European Union and European Economic Area .............................................................................. 847.2.3 Eurasian Economic Union

Евразийский экономический союз .................................................................................................. 85

Table of Contents

2 Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29025606 AD

7.2.4 Regulations for North America ............................................................................................................... 877.2.5 Regulatory statements .............................................................................................................................. 887.2.6 Declaration of Hazardous Substances (DoHS) ................................................................................ 89

Index ........................................................................................................................... 91

Table of Contents

Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29025606 AD 3

1 Introduction

About this chapterThis chapter contains important user information, description of safety notices and ageneral description of the intended use of Fraction collector F9-C and Fractioncollector F9-R.

In this chapter

Section See page

1.1 About this manual 5

1.2 Important user information 6

1 Introduction


1.1 About this manual

Purpose of this manualThe Operating Instruction manual provides information needed to install, operate andmaintain the product in a safe way.

Typographical conventionsSoftware items are identified in the text by bold italic text.

Hardware items are identified in the text by bold text.

In electronic format, references in italics are clickable hyperlinks.

1 Introduction1.1 About this manual


1.2 Important user information

Read this before operating theproduct

All users must read the entire Operating Instructions before installing, oper-ating or maintaining the product.

Always keep the Operating Instructions at hand when operating the product.

Do not install, operate, or perform maintenance on the product in any other way thandescribed in the user documentation. If you do, you may be exposed or expose othersto hazards that can lead to personal injury and you may cause damage to the equip-ment.

Intended useFraction collector F9-C and Fraction collector F9-R are automated fraction collectorsintended for the collection of fractions from purification runs. Fraction collector F9-Cand Fraction collector F9-R are intended for research use only, and shall not be used inclinical procedures, or for diagnostic purposes.

PrerequisitesIn order to operate Fraction collector F9-C and Fraction collector F9-R safely andaccording to the intended purpose the following prerequisites must be met:

• You should understand the concepts of liquid chromatography.

• You should be familiar with the purification instrument and system and have readthe Operating Instructions.

• You must read and understand the Safety chapter of these Operating Instructions.

• The system must be installed according to the instructions in Chapter 4 Installation,on page 39.

DefinitionsThis user documentation contains safety notices (WARNING, CAUTION, and NOTICE)concerning the safe use of the product. See definitions below.

1 Introduction1.2 Important user information


WARNING

WARNING indicates a hazardous situation which, if not avoided,could result in death or serious injury. It is important not to proceeduntil all stated conditions are met and clearly understood.

CAUTION

CAUTION indicates a hazardous situation which, if not avoided,could result in minor or moderate injury. It is important not toproceed until all stated conditions are met and clearly understood.

NOTICE

NOTICE indicates instructions that must be followed to avoiddamage to the product or other equipment.

Notes and tipsNote: A note is used to indicate information that is important for trouble-free and

optimal use of the product.

Tip: A tip contains useful information that can improve or optimize your proce-dures.

1 Introduction1.2 Important user information


2 Safety instructions

About this chapterThis chapter describes safety precautions and emergency shutdown procedures forFraction collector F9-C and Fraction collector F9-R. The labels on the fraction collec-tors are also described.

In this chapter

Section See page

2.1 Safety precautions 9

2.2 Symbols and abbreviations 15

2.3 Emergency procedures 17

2 Safety instructions


2.1 Safety precautions

About this sectionThe safety precautions in this section are grouped in the following categories:

• General precautions, on page 9

• Flammable liquids, on page 10

• Personal protection, on page 11

• Installing and moving the fraction collector, on page 11

• Operation, on page 12

• Maintenance, on page 13

Unless stated otherwise, the precautions apply to both Fraction collector F9-C andFraction collector F9-R.

General precautions

WARNING

Do not operate Fraction collector F9-C and Fraction collector F9-Rin any other way than described in the Operating Instructions.

WARNING

Operation and user maintenance of Fraction collector F9-C andFraction collector F9-R should be performed by properly trainedpersonnel only.

WARNING

Accessories. Use only accessories supplied or recommended byCytiva.

WARNING

Do not use Fraction collector F9-C and Fraction collector F9-R if itis not working properly, nor if it has suffered any damage, forexample:

• damage to the power cord or its plug

• damage caused by dropping the equipment

• damage caused by splashing liquid onto it

2 Safety instructions2.1 Safety precautions


Flammable liquids

CAUTIONExplosion hazard during fractionation of flammable liquids.Do not use a covered fraction collector (Fraction collector F9-C) tofractionate flammable liquids. When using an open fractioncollector (Fraction collector F9-R), do not place the fractioncollector in a closed compartment and make sure that the roomventilation meets the local requirements.

Fraction collector F9-C

CAUTIONFire Hazard. Do not fractionate flammable liquids usingFraction collector F9-C. When running RPC methods, or otherprocedures using organic solvents, collect fractions through theoutlet valve or Fraction collector F9-R.

CAUTIONExplosion hazard if flammable liquid leaks during cleaning ofthe flow path. When cleaning the flow path of Fraction collectorF9-C with a flammable liquid like ethanol, carefully inspect the flowpath, including the waste tubing, to make sure there will be noleakage.

Fraction collector F9-R

CAUTIONFractionation with flammable liquids using Fractioncollector F9-R. Before you start the run, make sure that the tubesin the fraction collector are of correct size according to fractionsize settings.

CAUTION

Moving tubes with flammable liquids. Move tubes filled withflammable liquids carefully, to prevent spillages that may come incontact with an ignition source.



Personal protection

CAUTION

Always use appropriate personal protective equipment duringoperation and maintenance of Fraction collector F9-C and Fractioncollector F9-R.

CAUTION

Hazardous substances. When using hazardous chemical andbiological agents, take all suitable protective measures, such aswearing protective glasses and gloves resistant to the substancesused. Follow local and/or national regulations for safe operation,maintenance and decommissioning of the equipment.

Installing and moving the fractioncollector

WARNING

UniNet cable. Only use UniNet cables delivered or approved byCytiva.

CAUTION

Heavy object. Use proper lifting equipment, or use two or morepersons when moving Fraction collector F9-C. All lifting andmoving must be performed in accordance with local regulations.

NOTICE

Vents on Fraction collector F9-C . To ensure adequate ventila-tion, keep papers and other objects away from the vents of thefraction collector.



Operation

CAUTION

Risk of breaking test vials. Do not use excessive force to pressvials with incorrect dimensions into the fraction collector. Glassvials may break and cause injuries.

CAUTION

Avoid spillage and overflow. Make sure that the equipment isprepared according to the settings in the method to be run.


WARNING

Moving parts. Do not open the door of Fraction collector F9-Cwhile the fraction collector is active. If you need to access the frac-tion collector, press Pause on the chromatography instrument,and make sure that no parts are moving before opening the door.

CAUTION

Maximum weight on the top of Fraction collector F9-C. Do notplace more than 30 kg on top of Fraction collector F9-C.


CAUTIONReplacement of the fraction collector bowl in Fractioncollector F9-R. Do not replace the bowl during a run.

CAUTION

Replacing tubes. Do not replace the tubes in the fractioncollector during a run.



Maintenance

CAUTION

Electrical shock hazard. All repairs should be done by servicepersonnel authorized by Cytiva. Do not open any covers or replaceparts unless specifically stated in the user documentation.

CAUTION

Disconnect power. Always switch off power to Fraction collectorF9-C and Fraction collector F9-R, before cleaning any of its compo-nents, unless stated otherwise in the user documentation. This isdone by switching off the chromatographic instrument thatprovides Fraction collector F9-C and Fraction collector F9-R withpower.

CAUTION

Hazardous chemicals and biological agents. Before mainte-nance, service and decommissioning, wash Fraction collector F9-Cand Fraction collector F9-R with a neutral solution to make surethat any hazardous solvents and biological agents have beenflushed out from the fraction collector.

CAUTION

Always use appropriate personal protective equipment whendecommissioning the equipment.

CAUTIONCleaning Fraction collector F9-C and Fraction collector F9-Rbefore decommissioning.

• Wipe Fraction collector F9-C and Fraction collector F9-R with adamp tissue using a cleaning agent so that no hazardoussolvents or biological agents remain on the surface. Do notforget to clean the interior of Fraction collector F9-C.

• Perform a system CIP using a neutral solution. Make sure thatany hazardous solvents or biological agents are flushed outfrom the fraction collector.



NOTICE

Cleaning. Keep Fraction collector F9-C and Fraction collector F9-R dry and clean. Wipe regularly with a soft damp tissue and, ifnecessary, a mild cleaning agent. Let Fraction collector F9-C andFraction collector F9-R dry completely before use.




2.2 Symbols and abbreviations

IntroductionThis section describes the information on the system label and other safety or regula-tory labels that are attached to the product.

System labelThe system label is located on the back of the equipment. The system label identifiesthe equipment and shows electrical data, regulatory compliance, and warningsymbols.

Labels on Fraction collector F9-C

Fraction collector F9-C safety labelIt is not allowed to fractionate flammable liquids using Fraction collector F9-C due tothe risk of build-up of flammable and explosive gases. When running RPC methods, orother procedures using organic solvents, collect fractions through the Outlet valve orwith Fraction collector F9-R.

The safety label below is located on the inside of the door of Fraction collector F9-C.

Do NOT fractionate�ammable liquids.

Labels on Fraction collector F9-RThe system label is located on the back of the equipment. The system label identifiesthe equipment and shows electrical data, regulatory compliance, and warningsymbols.

Label informationThe label contains information on the serial number, regulatory compliance symbols,recycling symbols, and specifications.

The label information is explained in the table below.

Label text Meaning

Code no Fraction collector code number

Serial no Fraction collector serial number

Mfg Year Manufacturing year and month

Voltage Supply voltage

2 Safety instructions2.2 Symbols and abbreviations


Label text Meaning

Max Power Max power consumption

Protection class Protection class, ingress protection according to IEC60529

Warning! Read the Operating Instruction beforeusing the module. Do not open any covers or replaceparts unless specifically stated in the OperatingInstructions.

This symbol indicates that electrical and electronicequipment must not be disposed of as unsortedmunicipal waste and must be collected separately.Please contact an authorized representative of themanufacturer for information concerning thedecommissioning of equipment.

This symbol indicates that the product might containhazardous materials in excess of the limits estab-lished by the Chinese standard SJ/T11363-2006Requirements for Concentration Limits for CertainHazardous Substances in Electronic InformationProducts.

The fraction collector complies with applicable Euro-pean directives

The equipment complies with the applicable require-ments for Australia and New Zealand

Eurasian Conformity mark: the single conformitymark indicates that the product is approved forcirculation on the markets of the member states ofthe Eurasian Customs Union.

CAN ICES-1/NMB-1 CAN ICES-1/NMB-1 indicates that this productcomplies with the Canadian standard ICES-001concerning technical requirements relative to radi-ated noise emissions from Industrial, Scientific andMedical radio frequency generators.

2 Safety instructions2.2 Symbols and abbreviations


2.3 Emergency procedures

About this sectionThe ÄKTA™ instrument supplies the Fraction collector F9-C and Fraction collector F9-R with power. This section describes how to perform an emergency shutdown of theFraction collector F9-C and Fraction collector F9-R by shutting down the ÄKTA instru-ment. This section also describes the results in the event of power failure or networkinterruption.

Emergency shutdownIn an emergency situation, stop the run by either pausing the run or switching off theinstrument as described below:

If you wantto...

then...

pause the run • press the Pause button on the Instrument control panel:

Note:

The buttons on the Instrument control panel may be locked.This is an option available in the System settings.

or

• click the Pause icon in UNICORN™:

Result: All pumps in the instrument are stopped.

2 Safety instructions2.3 Emergency procedures


If you wantto...

then...

switch off theinstrument

• press the Power switch to the 0 position, or

• disconnect the power cord from the wall socket.

Result: The run is interrupted immediately.

Note:

The sample and data may be lost as a result of switching off thepower.

Power failureIn case of a power failure or if communication is lost, the run is interrupted immediatelyand all moving parts in the fraction collector are stopped.

2 Safety instructions2.3 Emergency procedures


3 Fraction collector overview

About this chapterThis chapter gives overviews of Fraction collector F9-C and Fraction collector F9-R.

In this chapter

Section See page

3.1 Fraction collector F9-C 20

3.2 Fraction collector F9-R 31

3 Fraction collector overview


3.1 Fraction collector F9-C

About this sectionThis section shows an overview of Fraction collector F9-C.

Technical details are found in the ÄKTA pure User manual.

In this section

Section See page

3.1.1 Function 21

3.1.2 Fraction collector F9-C illustrations 22

3.1.3 Cassettes, Cassette tray and racks 26

3.1.4 Connect tubing to the ÄKTA pure instrument 30

3 Fraction collector overview3.1 Fraction collector F9-C


3.1.1 FunctionThe fraction collector collects fractions from ÄKTA pure purification runs.

The fraction collector can be used for:

• Fixed volume fractionation

• Peak fractionation

• Combined fixed volume fractionation and peak fractionation

Fraction collector F9-C has the following functions for reducing sample spill duringfractionation:

• Drop Sync

• Accumulator


3.1.1 Function


3.1.2 Fraction collector F9-C illustrations

IntroductionThis section provides illustrations of Fraction collector F9-C. The main features andcomponents are indicated.

Front viewThe illustration below shows the main parts of the exterior of Fraction collector F9-C.

1 2 3 5 64

Part Description

1 Fractionation indicator

Symbol indicating that fractionation is ongoing. Do not open the doorwhile the indicator is lit.

2 Door

3 Window

4 Door handle

5 Tubing connector for outlet valve tubing

6 Vents




Rear viewThe illustration below shows the rear view of Fraction collector F9-C.

1

1

23

Part Description

1 Vents

2 UniNet-9 D-type connector (for communication and power supply)

3 Waste tube




InteriorThe illustration below shows the main parts of the interior of Fraction collector F9-C.

1

2

3 4 5 6 7 8

9

10

11

12

1314

Part Description

1 Fractionation arm guide rail

2 Fractionation arm main rail

3 Lamp

4 Tubing guide

5 Tubing connection

6 Dispenser head

7 Fractionation arm

8 Tubing guide

9 Height exclusion bar

10 Waste funnel

11 Waste tube

12 Tray catch




Part Description

13 Waste groove, in case of overflow

14 Tray guides

Dispenser headThe illustration below shows the Dispenser head of Fraction collector F9-C.

1 2 3

4

56

Part Description

1 Dispenser head

2 Nozzle

3 Dispenser head cover

4 Accumulator (back part of Dispenser head)

5 Drop sync sensor

6 Type code reader




3.1.3 Cassettes, Cassette tray and racks

IntroductionFractions can be collected in deep well plates and in tubes of different sizes. A numberof cassettes, trays and racks for different tubes and deep well plates are available. Thecassettes are placed on a tray with six cassette positions.

The cassette type codes, tray type code and rack type codes are scanned by the typecode reader to determine the type of cassettes, trays and racks used. For more infor-mation on requirements that need to be fulfilled by the tubes and deep well plates to beused in Fraction collector F9-C, see the ÄKTA pure User manual.

Available cassettes, trays and racksThe following Cassettes and racks are available:

• Cassette 3 mL tubes (for 40 tubes)





• Cassette for deep well plate (24, 48, 96 wells)

• Cassette tray (for six cassettes)

• Rack for 50 mL tubes (for 55 tubes)

• Rack for 250 mL bottles (for 18 bottles)

For information on dimension requirements for tubes and deep well plates to be usedin the fraction collector, see Fraction collector tubes and bottles, on page 28 and Deepwell plates, on page 28 respectively.

Illustrations of Fraction collector F9-C tray and racks

The illustrations below show the Cassette tray, the Rack for 50 mL tubes and the Rackfor 250 mL bottles.

The fronts of the tray and the racks are marked with the Cytiva-logotype.

In the Cassette tray, the cassette positions are marked 1 to 6.




Cassette tray Rack for 50 mL tubes Rack for 250 mL bottles

Note: The tray and racks are inserted into the fraction collector with the Cytiva-logotype facing outwards.

Note: Do not use the Cassette tray when a rack for tubes or bottles is placed in thefraction collector.

Illustration of Cassettes on theCassette tray

The illustration below shows Cassettes placed on the Cassette tray.

Part Description

1 Cassette tray

2 Cassette position number

3 Cassette

4 Cassette type code

5 Tubes or deep well plates placed in a Cassette




Fraction collector tubes and bottlesThe tubes and bottles used in Fraction collector F9-C must fulfill the requirementslisted in the table below. Examples of manufacturers are also listed in the table.

Tube orbottle size(ml)

Diameter (mm) Height (mm) Examples ofmanufacturers

Min. Max. Min. Max.

3 10.5 11.5 50 56 Nunc™

5 10.5 12 70 76 VWR™

8 12 13.3 96 102 BD Biosciences,VWR

15 16 17 114 120 BD Biosciences

50 28 30 110 116 BD Biosciences

250 mL bottle L: 55W: 551

L: 64.5W: 641

- 121 Nalgene™ ,Kautex™

1 Length and width of the rectangular bottle base

Deep well plates

RequirementsThe deep well plates used in Fraction collector F9-C must fulfill the requirements listedin the table below.

Property Specification

No. of wells 24, 48, or 96

Shape of wells Square, not cylindrical

Well volume 10, 5, or 2 mL

Approved deep well platesThe plates listed in the table below are tested and approved by Cytiva to be used withFraction collector F9-C.

Plate type Manufacturer Part no.

96 deep well plate Cytiva 7701-5200 (Whatman™)

BD Biosciences 353966




Plate type Manufacturer Part no.

Greiner Bio-One 780270

Porvair Sciences 219009

Seahorse Bioscience S30009

Eppendorf™ 951033405/0030 501.306

48 deep well plate Cytiva 7701-5500 (Whatman)


24 deep well plate Cytiva 7701-5102 (Whatman)





3.1.4 Connect tubing to the ÄKTA pure instrument


Fraction collector F9-C is delivered with all internal tubing in place. The tubing betweenthe fraction collector and purification instrument need to be installed.

Follow the instructions in the table below to connect the tubing from the ÄKTA pureinstrument to the fraction collector.

Step Action

1 Connect the tubing Frac to the Frac port on the outlet valve on the ÄKTApure instrument.

2 Connect the other end of the Frac tubing to the inlet port on the fractioncollector.

3 Adjust the delay volume setting in UNICORN, see Section 4.4 Set the delayvolume, on page 57 for more details.


3.1.4 Connect tubing to the ÄKTA pure instrument


3.2 Fraction collector F9-R

About this sectionThis section shows an overview of Fraction collector F9-R.

Technical details are found in the ÄKTA pure User manual and ÄKTA avant Usermanual.

In this section

Section See page

3.2.1 Function 32

3.2.2 Fraction collector F9-R illustrations 33

3.2.3 Tubes 36

3.2.4 Connect tubing to ÄKTA pure or ÄKTA avant 38

3 Fraction collector overview3.2 Fraction collector F9-R


3.2.1 FunctionThe fraction collector collects fractions from purification runs on ÄKTA pure and ÄKTAavant instruments.

The fraction collector can be used for:

• Fixed volume fractionation

• Peak fractionation

• Combined fixed volume fractionation and peak fractionation

• Collecting fractions in reversed phase separations using organic solvents

Fraction collector F9-R has the following function for reducing sample spill during frac-tionation:

• Drop Sync


3.2.1 Function


3.2.2 Fraction collector F9-R illustrations

IntroductionThis subsection provides illustrations of Fraction collector F9-R. The main features andcomponents are indicated.




Front viewThe illustration below shows the main parts of the Fraction collector.

1

3 4

5

7

6

8

2

Part Function

1 Lock knob

2 Stationary part of delivery arm

3 Delivery arm

4 Tubing connector

5 Tube sensor

6 Collection tubes

7 Tube rack

8 Base unit




Connector panelThe illustration below shows the main parts of the connector panel on the fractioncollector.

1 22

Part Function

1 Node ID switch

2 UniNet-9 F-type connector (for communication and power supply)




3.2.3 Tubes

IntroductionFor Fraction collector F9-R the fractions are collected in tubes of different sizes. Formore information on requirements that need to be fulfilled by the tubes to be used inFraction collector F9-R, see the ÄKTA pure User manual or the ÄKTA avant Usermanual.

Available tubesTubes with the following diameter can be used with Fraction collector F9-R:

• 12 mm

• 18 mm

• 30 mm

The tubes can have a tube length between 50-180 mm.


3.2.3 Tubes


Illustration of the Fraction collectorF9-R Tube rack

Each tube rack is made up of a combination of a Bowl, Tube support, Tube guide andTube holder. For more information on the assembly of the tube rack, see Assemble theTube rack, on page 68. For information on which Tube rack to use, see the ÄKTA pureUser manual or the ÄKTA avant User manual.

1

2

3456

Part Function

1 Single cutout

2 L-shaped cutout

3 Bowl

4 Tube support

5 Tube guide

6 Tube holder

Note: Note that the tube guide has both single and L-shaped cutouts, while thetube holder only has single cutouts. See Prepare the fraction collector, onpage 68 for more information.


3.2.3 Tubes


3.2.4 Connect tubing to ÄKTA pure or ÄKTA avant

Follow the instructions in the table below to connect the tubing from the fractioncollector to ÄKTA pure. For connecting the tubing to ÄKTA avant, see the ÄKTA avantUser manual.

Step Action

1 Lift out the Tubing holder (4) from the Delivery arm (1).

3

4

5

6

2 Loosen the nut of the Tubing holder. Do not remove the Tubing holder nut (5)from the Tubing holder.

3 Insert the tubing through the Tubing holder.

4 Place the Tubing holder with the tubing over the Tube adjustment cavity (2)of the Delivery arm. Push the tubing down against the bottom of the Tubeadjustment cavity, and then fingertighten the Tubing holder nut. Thisensures the correct length of the exposed tubing end (6).

5 Re-install the tubing holder in the Delivery arm.

6 For Fraction collector F9-R: Connect the tubing from the fraction collectorto the port Frac on the outlet valve.

For Fraction collector F9-R, 2nd: Connect the tubing from the secondfraction collector to:

• port Out 10 on Outlet valve V9-O or V9H-O

• port Out 1 on Outlet valve V9-Os or V9H-Os

7 Adjust the delay volume setting in UNICORN to the volume of the tubing, see Section 4.4 Set the delay volume, on page 57 for more details.


3.2.4 Connect tubing to ÄKTA pure or ÄKTA avant


4 Installation

About this chapterThis chapter provides required information to enable users and service personnel toinstall, move and transport Fraction collector F9-C and Fraction collector F9-R.

In this chapter

Section See page

4.1 Room and site requirements 40

4.2 Connecting two fraction collectors to ÄKTA pure 46

4.3 Prepare waste tubing for Fraction collector F9-C 55

4.4 Set the delay volume 57

4.5 Performance test 59

4 Installation


4.1 Room and site requirements

About this sectionThis section describes the site planning and the preparations necessary for the instal-lation of Fraction collector F9-C and Fraction collector F9-R. The purpose is to provideplanners and technical staff with the data needed to prepare the laboratory for theinstallation.

In this section

Section See page

4.1.1 Delivery and storage 41

4.1.2 Placement and space requirements 42

4.1.3 Site requirements 45

4 Installation4.1 Room and site requirements


4.1.1 Delivery and storage

About this sectionThis section describes the requirements for receiving the delivery box and storingFraction collector F9-C and Fraction collector F9-R before installation.

When you receive the delivery

• Record on the receiving documents if there is any apparent damage on the deliverybox. Inform your Cytiva representative of such damage.

• Move the delivery box to a protected location indoors.

Storage requirementsThe delivery box should be stored in a protected place indoors. The following storagerequirements must be fulfilled for the unopened box:

Parameter Allowed range

Ambient temperature,storage

-25°C to 60°C

Relative humidity up to 90% atmospheric humidity at 40°C for 48 hrs


4.1.1 Delivery and storage


4.1.2 Placement and space requirements

About this sectionThis section describes different options of where to place Fraction collector F9-C andFraction collector F9-R and the space required.

LocationPlace the fraction collector on a clean, flat and stable area that is able to support theweight of the fraction collector.

The following locations are recommended for the Fraction collector F9-C and theFraction collector F9-R:

• on the bench to the left of the purification instrument

• on a shelf below the bench

Note: Longer tubing length increases back pressure and band broadening in thechromatographic process. Place the fraction collector so that the totaltubing length is minimized.

Space requirementsThe illustration below shows the space recommended for the system includingFraction collector F9-C when used with ÄKTA pure.




The illustration below shows the space recommended for the system includingFraction collector F9-R when used with ÄKTA pure.

Note: Fraction collector F9-R requires 10 cm free space above the fractionationarm for handling of tubes.

Fraction collector weight

Item Weight

Fraction collector F9-C 21 kg

Fraction collector F9-R 5 kg




Fraction collector dimensionsThe outer dimensions of Fraction collector F9-C are shown in the illustration below.

585 mm

390 mm

320 mm

The outer dimensions of Fraction collector F9-R are shown in the illustration below.




4.1.3 Site requirements

About this sectionThis section describes the site requirements for installation of Fraction collector F9-Cand Fraction collector F9-R.


4.1.3 Site requirements


4.2 Connecting two fraction collectors to ÄKTA pure

About this sectionThis section contains information on how to set up tubing and a connection for powerand communication between Fraction collector F9-C or Fraction collector F9-R andthe ÄKTA pure instrument.

Note: This information can apply to other ÄKTA purification instruments. Pleasecheck the purification instrument documentation for more information.

It is possible to install up to two fraction collectors in the following combinations whenusing ÄKTA pure:

• one Fraction collector F9-C and one Fraction collector F9-R, or

• two Fraction collector F9-R.

If two fraction collectors are to be used, the following applies to the second one:

• it must be a Fraction collector F9-R,

• it has to be configured as Fraction collector F9-R, 2nd,

• the node ID has to be changed, as described in Check/change Node ID, on page 51.

In this section

Section See page

4.2.1 Power and communication 47

4.2.2 Connect tubing 53

4 Installation4.2 Connecting two fraction collectors to ÄKTA pure


4.2.1 Power and communication

IntroductionThis subsection describes how to set up the power and communication connectionbetween Fraction collector F9-C or Fraction collector F9-R and the ÄKTA pure instru-ment.

Connect fraction collector

The following cables are used to connect the fraction collectors to the ÄKTA pureinstrument. Use the specified cable and follow the instructions below.

Fraction collector Cable

Fraction collector F9-C UniNet-9 cable, D-type

Fraction collector F9-R UniNet-9 cable, F-type

WARNING

UniNet cable. Only use UniNet cables delivered or approved byCytiva.

Step Action

1 Switch off the power to the ÄKTA pure instrument.




Step Action

2 Remove the jumper from the UniNet-9 port to be used on the rear of theÄKTA pure instrument (port 7 or 8 for Fraction collector F9-C, port 1 to 6 forFraction collector F9-R).

3 Connect the UniNet-9 cable between the UniNet-9 ports on the back of thefraction collector and on the back of the ÄKTA pure instrument.

NOTICE

Do not use the Test port on the ÄKTA pure instrument.


2





Step Action

4 Make sure that all unused UniNet-9 ports on the ÄKTA pure instrument areplugged with jumpers.

Software configuration

When the fraction collector has been installed, the System properties for the systemhas to be updated in UNICORN. The system will restart automatically when the config-uration has been changed and the system can be reconnected.

From hereon, UNICORN refers to UNICORN 6.3 or other compatible versions of thesoftware. The examples given in these Operating Instructions refer to UNICORN 6.3.

Follow the instructions below to update the system in UNICORN.




Step Action

1 • In the Administration module, choose Tools →System Properties orclick the System Properties icon to open the dialog.

Result:

The System Properties dialog is displayed.

• Select a system in the System Properties dialog.

Note:

Only active systems can be edited.

• Click the Edit button.

Result:

The Edit dialog is displayed.




Step Action

2 In the Edit dialog:

• Select Fraction collectors from the Component types list.

Result:

All available fraction collectors are shown in the Component selectionlist.

Note:

Instrument modules are referred to as Components in UNICORN.

Note:

An outlet valve has to be selected in order to select a fraction collector.

• Select the Fraction collector check box.

• Choose what kind of fraction collector to add from the drop down list box.

• Note:

Fraction collector 2 is only available in the Component selection list ifFraction collector is already selected.

3 Click the OK button to apply the changes.

Check/change Node IDThe Node ID for Fraction collector F9-C cannot be changed.

The Node ID for Fraction collector F9-R needs to be changed if

• the fraction collector will be used as secondary fraction collector, fractioncollector, 2nd.

• the fraction collector is to be used as primary fraction collector after it has beenused as secondary.

The Node ID for Fraction collector F9-R is set by positioning the arrow of the rotatingswitch at the back of the Fraction collector. Use a screwdriver to set the arrow of theswitch to the desired number.




Fraction collector Order Node ID

Fraction collector F9-C 1st 0

Fraction collector F9-R 1st 0

2nd 1




4.2.2 Connect tubing

IntroductionThis subsection contains information on how to connect tubing from ÄKTA pure toFraction collector F9-C and Fraction collector F9-R.


Fraction collector F9-C is delivered with all internal tubing in place. The tubing betweenthe fraction collector and purification instrument need to be installed.

Follow the instructions in the table below to connect the tubing from the ÄKTA pureinstrument to the fraction collector.

Step Action

1 Connect the tubing Frac to the Frac port on the outlet valve on the ÄKTApure instrument.

2 Connect the other end of the Frac tubing to the inlet port on the fractioncollector.

3 Adjust the delay volume setting in UNICORN, see Section 4.4 Set the delayvolume, on page 57 for more details.


Follow the instructions in the table below to connect the tubing from the fractioncollector to the ÄKTA pure instrument.




Step Action

1 Lift out the Tubing holder (4) from the Delivery arm (1).

3

4

5

6

2 Loosen the nut of the Tubing holder. Do not remove the Tubing holder nut (5)from the Tubing holder.

3 Insert the tubing through the Tubing holder.

4 Place the Tubing holder with the tubing over the Tube adjustment cavity (2)of the Delivery arm. Push the tubing down against the bottom of the Tubeadjustment cavity, and then fingertighten the Tubing holder nut. Thisensures the correct length of the exposed tubing end (6).

5 Re-install the tubing holder in the Delivery arm.

6 For Fraction collector F9-R: Connect the tubing from the fraction collectorto the port Frac on the outlet valve.

For Fraction collector F9-R, 2nd: Connect the tubing from the secondfraction collector to:

• port Out 10 on Outlet valve V9-O

• port Out 1 on Outlet valve V9-Os

7 Adjust the delay volume setting in UNICORN to the volume of the tubing, see Set the delay volume, on page 57 for more details.




4.3 Prepare waste tubing for Fraction collector F9-CThe waste outlet is located on the rear of the fraction collector. See instructions belowfor how to prepare the waste tubing.

Instructions

Follow the instructions below to prepare the waste tubing.

Step Action

1 Insert the waste tubing in a vessel.

NOTICE

The maximum level of the vessel for the waste tubingmust be lower than the fraction collector.

4 Installation4.3 Prepare waste tubing for Fraction collector F9-C


Step Action

2 Cut the waste tubing to appropriate length. It is important that the tubing isnot bent and will not be submerged in liquid during the run.

Note:

If the tubing is too short, replace it with new tubing. Do not lengthen thetubing as this might cause obstruction of the tubing.

3 Securely fasten the tubing to the waste vessel.

CAUTION

Make sure that the waste vessel will hold all the produced volumeof the run.

4 Installation4.3 Prepare waste tubing for Fraction collector F9-C


4.4 Set the delay volume

IntroductionThe delay volume settings are used to make sure that the fractions collected duringfractionation, using the outlet valve or the fraction collector, correspond to the frac-tions indicated in the chromatogram.

Set the delay volume

Follow the instructions below to set the delay volume between the UV monitor and theoutlet valve and between the UV monitor and the fraction collector. Refer to the purif-cation instrument User manual for more information about delay volumes.

Step Action

1 Select System →Settings in the System Control module.

Result:

The System Settings dialog opens.

2 • Select Tubing and Delay Volumes and select Delay volume →Monitorto outlet valve.

• Type in the volume in the Volume field and click OK.

4 Installation4.4 Set the delay volume


Step Action

3 In the System Settings dialog:

• Select Delay volume →Monitor to outlet valve or Monitor to fracaccording to the configuration you are using.

• Type in the volume in the Volume field and click OK.

Note:

The system will use the delay volume appropriate to the configuration usedand ignore other settings (e.g. the value for Monitor to outlet valve will beignored if you are using a fraction collector). It is however recommended toset all delay volumes so that the volumes remain correct if you change frac-tionation method.

4 Installation4.4 Set the delay volume


4.5 Performance testBefore taking the Fraction collector F9-C and Fraction collector F9-R instrument intouse, run a performance test to check the function of the equipment. See the purifcationinstrument User manual for further instructions.

4 Installation4.5 Performance test


5 Operation

About this chapterThis chapter describes how to prepare and assemble the fraction collector before arun.

In this chapter

Section See page

5.1 Prepare Fraction collector F9-C 61


5 Operation


5.1 Prepare Fraction collector F9-C

About this sectionThis section describes how to prepare and assemble Fraction collector F9-C before arun.

The fraction collector is connected to ÄKTA pure and controlled by UNICORN. Controlof the fraction collector can be achieved automatically in a method run, or manually.

CAUTIONFire Hazard. Do not fractionate flammable liquids usingFraction collector F9-C. When running RPC methods, or otherprocedures using organic solvents, collect fractions through theoutlet valve or Fraction collector F9-R.

Note: The tray and racks can tilt slightly when not fully inserted into the fractioncollector and may harm the fractionation arm. The tilt is due to the heightdifference between the door and the floor of the fraction collector. Thetendency to tilt is affected by the placement and weight of the cassettes,tubes or bottles

Prepare the fraction collectorBefore starting to prepare Fraction collector F9-C, check the fractionation settings inthe method to be run. Perform the steps described below according to the settings inthe method.

• Insert the Cassette tray or a rack for tubes or bottles.

• Change the System Settings in UNICORN to set the fractionation mode and othersettings for fraction collection.

How to insert a tray or a rack is shown below.

For information on how to change the System Settings before a run, see either theÄKTA pure User manual or the ÄKTA avant User manual. The available SystemSettings are described in the ÄKTA pure User manual.

Prepare and insert the Cassette tray

Follow the instructions below to add cassettes to the Cassette tray and insert the trayinto the fraction collector.

5 Operation5.1 Prepare Fraction collector F9-C


Step Action

1 If you are to use cassettes with the QuickRelease function, open thecassettes. For more information on the QuickRelease function see the ÄKTApure User manual.

2 Place the tubes and deep well plates in the cassettes. Make sure that thedeep well plates are rotated so that the well marked A1 is positioned abovethe A1 marking on the cassette.



Step Action

3 Close the cassettes that have the QuickRelease function.

4 Place the cassettes on the Cassette tray. Make sure that the cassette typecode (see illustration below) faces the front of the tray marked with theCytiva logo.

5 Open the door of the fraction collector using the handle.



Step Action

6 Insert the tray into the fraction collector:

• make sure that the front of the tray (marked with the Cytiva logo) facesoutwards

• position the tray and slide it into the fractionation collector until itreaches the end

• make sure that the Tray catch snaps into closed position, as shown below.

Note:

• The tray can tilt slightly when not fully inserted into the fraction collectorand may harm the fractionation arm. The tilt is due to the height differ-ence between the door and the floor of the fraction collector. Thetendency to tilt is affected by the placement and weight of the cassettes.

• A height exclusion bar ensures that the tubes or deep well plates arecorrectly positioned and cannot damage the Dispenser head.



Step Action

7 Close the door. Make sure that it closes properly.

Result:

After the door has been closed, the fractionation arm scans the cassettetype code of each cassette to identify the cassette types. If deep well platesare used, the instrument also identifies the types of deep well plates.

Note:

If the tray is inserted with the front of the tray facing the wrong way it will notbe possible to close the door.

Prepare and insert a rack for tubes orbottles

Follow the instructions below to insert a rack for tubes or bottles into the fractioncollector.

Step Action

1 Place the tubes or bottles in the rack.

2 Open the door of the fraction collector using the handle.



Step Action

3 Insert the rack into the fraction collector:

• make sure that the front of the rack (marked with the Cytiva logo) facesoutwards

• position the rack and slide it into the fractionation collector until itreaches the end

• make sure that the Tray catch snaps into closed position, as shown below.

Note:

• The rack can tilt slightly when not fully inserted into the fraction collectorand may harm the fractionation arm. The tilt is due to the height differ-ence between the door and the floor of the fraction collector. Thetendency to tilt is affected by the placement and weight of the tubes orbottles.

• A height exclusion bar ensures that the tubes or bottles are correctly posi-tioned and cannot damage the Dispenser head.

• Do not use the Cassette tray when the Rack for 50 ml tubes or the Rack for250 ml bottles is placed in the fraction collector.



Step Action

4 Close the door. Make sure that it closes properly.

Note:

If the rack is inserted with the front of the rack facing the wrong way it will notbe possible to close the door.




About this sectionThis section describes how to prepare and assemble Fraction collector F9-R before arun.

The Fraction collector F9-R is connected to either ÄKTA pure or ÄKTA avant andcontrolled by UNICORN. Control of the fraction collector can be achieved automati-cally in a method run, or manually.

Prepare the fraction collectorBefore starting to prepare Fraction collector F9-R, check the fractionation settings inthe method to be run. Perform the steps described below according to the settings inthe method.

• Assemble the tube rack

• Insert collection tubes

• Adjust the Delivery arm

• Change the System Settings in UNICORN to set the fractionation mode and othersettings for fraction collection.

It is shown how to assemble the tube rack, insert collection tubes and adjust thedelivery arm below. For more information on how to change the System Settingsbefore a run, see either the ÄKTA pure User manual or the ÄKTA avant User manual.

Assemble the Tube rack

Follow the instructions below to assemble the Tube rack. See either the ÄKTA pureUser manual or the ÄKTA avant User manual for information about which Tube rackparts to use.

5 Operation5.2 Fraction collector F9-R


Step Action

1 Insert the Tube support (4), if required, into the bowl (3). The circular markson the Tube support should face down.

1

2

3456

Note:

When assembling a Tube rack, Single cutouts (1) and L-shaped cutouts (2),are used for various inserts depending on the length of the collection tubes.See either the ÄKTA pure User manual or the ÄKTA avant User manual fordetailed information.

2 Insert the Tube guide (5) with the tube position numbers upwards. The Tubeguide should rest about 1 cm above the Tube support.



Step Action

3 Insert the Tube holder (6) with the tube position numbers upwards:

• Check that tube position 1 (7) is directly above tube position 1 (7) of theTube guide.

7

• Push the flexible bowl out at each rib and snap the Tube holder under thetop lip of the rib (8).

8

Note:

Do not force the tube holder into place as this may damage the lip.

• Check that the surface of the Tube holder is level.

4 Gently move the Delivery arm (10) out to the outer stop.



Step Action

5 Place the Tube rack (9) over the Central spindle (11) and pull the springloaded Drive sleeve (12) out so the Tube rack comes to rest.

9

1012

11

Insert collection tubesInsert sufficient collection tubes in to the Tube rack, starting at position 1, pushingeach one down as far as they will go. All the tubes must be of the same length and diam-eter and there should be no empty positions in the sequence.

Adjust the Delivery arm

Follow the instructions in the table below to adjust the Delivery arm.



Step Action

1 Gently lift and then lower the Delivery arm (1), and allow it to move in so theTube sensor (2) touches the collection tubes of the outer track.

1

2



Step Action

2 Adjust the height of the Delivery arm as follows:

• Loosen the lock knob (3)

3

• Adjust the height so that the horizontal mark (5) on the Tube sensor (6) isat the same level as the top of the flat collection tubes and approximately2 mm over the top of the flanged collection tubes (4).

4

5

6

• Lock the Delivery arm at this height with the lock knob.



Step Action

3 Check that the Tube sensor (9) is in the correct position for the used tubes(10). The eluent tubing (7) should be above the center of the collection tube.Use the Sensor control (8) to position the Tube holder.

7

8

9

10



6 Maintenance

About this chapterThis chapter describes maintenance and provides instructions for how to cleanFraction collector F9-C and Fraction collector F9-R. For further information, see thepurifcation instrument User manual.


In this chapter

Section See page

6.1 Clean Fraction collector F9-C 76


6 Maintenance


6.1 Clean Fraction collector F9-C

Maintenance intervalClean the Fraction collector when required, for example if liquid has been spilled in theFraction collector chamber. The internal tubing of the fraction collector may need to bereplaced for maintenance or for process purposes. Information on how and when toreplace the internal tubing can be found in the ÄKTA pure User manual.

Required materialThe following materials are required:

• Wash bottle

• Water or 20% ethanol

• Cloth

Instruction

Follow the instruction below to clean the interior of the Fraction collector. The loca-tions of the components of the fraction collector are shown in Section 3.1.2 Fractioncollector F9-C illustrations, on page 22.

Step Action

1 Perform a fraction collector wash:

In System Control, select Manual →Execute Manual Instructions→Fraction collection →Fraction collector wash. Click Execute.

NOTICE

• If no column valve is used, make sure to replace anycolumns in the flow path with tubing before a fractioncollector wash is performed.

• Lower the flow for the fraction collector wash in theinstruction Fraction collector wash settings if thesystem back pressure is elevated during the wash.

• Make sure not to exceed the pressure limits for any ofthe modules that are part of the flow path.

2 In System Control, select Manual →Execute Manual Instructions→Fraction collection →Frac cleaning position . Click Execute.

Result:

The Dispenser head moves to cleaning position, and the Instrument displaystates System pause.

6 Maintenance6.1 Clean Fraction collector F9-C


Step Action

3 Open the door of the fraction collector and remove the rack

4 Wash the Cassette tray or rack and the Cassettes (if applicable), with waterand a mild cleaning agent.

5 Lift off the Waste funnel and wash it with water and a mild cleaning agent.

Refit the Waste funnel.

6 Wipe off the interior of the fraction collector using a damp cloth. Wipe offstains using a mild cleaning agent or 20% ethanol.

7 Wipe off the Dispenser head and its diode windows (the Drop sync sensorand the Type code reader) using a wash bottle with water or 20% ethanoland a cloth.

8 Let the fraction collector dry completely before starting a run.

9 Close the door of the fraction collector.

Result:

Automatic scanning is performed.

10 In the System Control module, press the End icon in the toolbar.

Result:

The Dispenser head moves to home position.

6 Maintenance6.1 Clean Fraction collector F9-C



Maintenance intervalClean the Fraction collector when required, for example in case of liquid spill.

Required materialThe following material are required:

• Water or 20% ethanol

• Cloth

Instruction

Follow the instructions below to clean the instrument externally.

Step Action

1 Check that no run is in progress.

2 Switch off the instrument.

3 Wipe the surface with a damp cloth. Wipe off stains using a mild cleaningagent or 20% ethanol. Wipe off any excess.

4 Let the Fraction collector F9-R dry completely before restart.

6 Maintenance6.2 Fraction collector F9-R


7 Reference information

See the relevant purification instrument Product information and User manual forinformation on the following:

• Technical specifications

• Chemical resistance

For information on operating conditions, see Section 4.1.3 Site requirements, on page45.

In this chapter

Section See page

7.1 Recycling information 81

7.2 Regulatory information 82

Environmental ranges

Parameter Data

Storage and transport temperaturerange

-25°C to +60°C

Chemical environment See the relevant purification instrumentUser manual.

Equipment noise level

Equipment Acoustic noiselevel

Fraction collector F9-C < 60 dB A

Fraction collector F9-R < 60 dB A



Heat output

Equipment Heat output

Fraction collector F9-C Typically 6 W

Maximum 18 W

Fraction collector F9-R 2 W



7.1 Recycling information

IntroductionThis section contains information about the decommisioning of Fraction collector F9-C and Fraction collector F9-R.

DecontaminationThe product must be decontaminated before decommissioning. All local regulationsmust be followed with regard to scrapping of the equipment.

Disposal of the productWhen taking the product out of service, the different materials must be separated andrecycled according to national and local environmental regulations.

CAUTION

Always use appropriate personal protective equipment whendecommissioning the equipment.

Disposal of electrical componentsWaste comprising electrical and electronic equipment must not be disposed of asunsorted municipal waste and must be collected separately. Please contact an author-ized representative of the manufacturer for information concerning the decommis-sioning of equipment.

7 Reference information7.1 Recycling information


7.2 Regulatory information

IntroductionThis section lists the regulations and standards that apply to the product.

In this section

Section See page

7.2.1 Contact information 83

7.2.2 European Union and European Economic Area 84

7.2.3 Eurasian Economic UnionЕвразийский экономический союз

85

7.2.4 Regulations for North America 87

7.2.5 Regulatory statements 88

7.2.6 Declaration of Hazardous Substances (DoHS) 89

7 Reference information7.2 Regulatory information


7.2.1 Contact information

Contact information for supportTo find local contact information for support and sending troubleshooting reports, visit cytiva.com/contact.

Manufacturing informationThe table below summarizes the required manufacturing information.

Requirement Information

Name and address of manufacturer Cytiva Sweden AB

Björkgatan 30

SE 751 84 Uppsala

Sweden

Telephone number of manufacturer + 46 771 400 600


7.2.1 Contact information


http://www.cytiva.com/contact

7.2.2 European Union and European Economic Area

IntroductionThis section describes regulatory information for the European Union and EuropeanEconomic Area that applies to the equipment.

Conformity with EU DirectivesSee the EU Declaration of Conformity for the directives and regulations that apply forthe CE marking.

If not included with the product, a copy of the EU Declaration of Conformity is availableon request.

CE marking

The CE marking and the corresponding EU Declaration of Conformity is valid for theinstrument when it is:

• used according to the Operating Instructions or user manuals, and

• used in the same state as it was delivered, except for alterations described in theOperating Instructions or user manuals.


7.2.2 European Union and European Economic Area


7.2.3 Eurasian Economic UnionЕвразийский экономический союз

This section describes the information that applies to the product in the EurasianEconomic Union (the Russian Federation, the Republic of Armenia, the Republic ofBelarus, the Republic of Kazakhstan, and the Kyrgyz Republic).

IntroductionThis section provides information in accordance with the requirements of the Tech-nical Regulations of the Customs Union and (or) the Eurasian Economic Union.

ВведениеВ данном разделе приведена информация согласно требованиям Техническихрегламентов Таможенного союза и (или) Евразийского экономического союза.

Manufacturer and importerinformation

The following table provides summary information about the manufacturer andimporter, in accordance with the requirements of the Technical Regulations of theCustoms Union and (or) the Eurasian Economic Union.

Requirement Information

Name, address and telephonenumber of manufacturer

See Manufacturing information

Importer and/or company forobtaining information aboutimporter

LLC Global Life Sciences Solutions Rus

Russian Federation, 123112

Presnenskaya nab., 10, fl. 12, pr. III, room 6

Telephone: + 7 495 739 6931

Fax nr: + 7 495 739 6932

E-mail: [email protected]

Информация о производителе иимпортере

В следующей таблице приводится сводная информация о производителе иимпортере, согласно требованиям Технических регламентов Таможенного союзаи (или) Евразийского экономического союза.

Требование Информация

Наименование, адрес и номертелефона производителя

См. Информацию об изготовлении


7.2.3 Eurasian Economic Union

Евразийский экономический союз


mailto:[email protected]

Требование Информация

Импортер и/или лицо дляполучения информации обимпортере

ООО "Глобал Лайф Сайэнсиз СолюшнзРус"

Российская Федерация, 123112

Пресненская наб., д. 10, эт. 12, пом. III,ком. 6

Телефон: + 7 495 739 6931

Факс: + 7 495 739 6932

Адрес электронной почты: [email protected]

Description of symbol on the systemlabelОписание обозначения на этикеткесистемы

This Eurasian compliance mark indicates that the product isapproved for use on the markets of the Member States of theCustoms Union of the Eurasian Economic Union

Данный знак о Евразийском соответствии указывает, чтоизделие одобрено для использования на рынкахгосударств-членов Таможенного союза Евразийскогоэкономического союза


7.2.3 Eurasian Economic Union

Евразийский экономический союз


mailto:[email protected]

7.2.4 Regulations for North America

IntroductionThis section describes the information that applies to the product in the USA andCanada.

FCC complianceThis device complies with part 15 of the FCC Rules. Operation is subject to the followingtwo conditions: (1) This device may not cause harmful interference, and (2) this devicemust accept any interference received, including interference that may cause unde-sired operation.

Note: The user is cautioned that any changes or modifications not expresslyapproved by Cytiva could void the user’s authority to operate the equip-ment.

This equipment has been tested and found to comply with the limits for a Class A digitaldevice, pursuant to part 15 of the FCC Rules. These limits are designed to providereasonable protection against harmful interference when the equipment is operated ina commercial environment. This equipment generates, uses, and can radiate radiofrequency energy and, if not installed and used in accordance with the instructionmanual, may cause harmful interference to radio communications. Operation of thisequipment in a residential area is likely to cause harmful interference in which case theuser will be required to correct the interference at his own expense.


7.2.4 Regulations for North America


7.2.5 Regulatory statements

IntroductionThis section shows regulatory statements that apply to regional requirements.

EMC emission, CISPR 11: Group 1,Class A statement

NOTICE

This equipment is not intended for use in residential environmentsand may not provide adequate protection to radio reception insuch environments.

South KoreaRegulatory information to comply with the Korean technical regulations.

NOTICE

Class A equipment (equipment for business use).

This equipment has been evaluated for its suitability for use in abusiness environment.

When used in a residential environment, there is a concern of radiointerference.

주의사항A급 기기 (업무용 방송통신 기자재)

이 기기는 업무용환경에서 사용할 목적으로 적합성평가를 받은 기기로서 가정용 환경에서 사용하는 경우 전파간섭의 우려가 있습니다.


7.2.5 Regulatory statements


7.2.6 Declaration of Hazardous Substances (DoHS)根据 SJ/T11364-2014《电子电气产品有害物质限制使用标识要求》特提供如下有关污染控制方面的信息。The following product pollution control information is provided according to SJ/T11364-2014 Marking for Restriction of Hazardous Substances caused by electricaland electronic products.

电子信息产品污染控制标志说明Explanation of Pollution ControlLabel

该标志表明本产品含有超过中国标准 GB/T 26572 《电子电气产品中限用物质的限量要求》中限量的有害物质。标志中的数字为本产品的环保使用期，表明本产品在正常使用的条件下，有毒有害物质不会发生外泄或突变，用户使用本产品不会对环境造成严重污染或对其人身、财产造成严重损害的期限。单位为年。为保证所申明的环保使用期限，应按产品手册中所规定的环境条件和方法进行正常使用，并严格遵守产品维修手册中规定的定期维修和保养要求。产品中的消耗件和某些零部件可能有其单独的环保使用期限标志，并且其环保使用期限有可能比整个产品本身的环保使用期限短。应到期按产品维修程序更换那些消耗件和零部件，以保证所申明的整个产品的环保使用期限。本产品在使用寿命结束时不可作为普通生活垃圾处理，应被单独收集妥善处理。This symbol indicates the product contains hazardous materials in excess of the limits estab-lished by the Chinese standard GB/T 26572 Requirements of concentration limits for certainrestricted substances in electrical and electronic products. The number in the symbol is the Envi-ronment-friendly Use Period (EFUP), which indicates the period during which the hazardoussubstances contained in electrical and electronic products will not leak or mutate under normaloperating conditions so that the use of such electrical and electronic products will not result inany severe environmental pollution, any bodily injury or damage to any assets. The unit of theperiod is “Year”.

In order to maintain the declared EFUP, the product shall be operated normally according to theinstructions and environmental conditions as defined in the product manual, and periodic main-tenance schedules specified in Product Maintenance Procedures shall be followed strictly.

Consumables or certain parts may have their own label with an EFUP value less than the product.Periodic replacement of those consumables or parts to maintain the declared EFUP shall be donein accordance with the Product Maintenance Procedures.

This product must not be disposed of as unsorted municipal waste, and must be collected sepa-rately and handled properly after decommissioning.


7.2.6 Declaration of Hazardous Substances (DoHS)


有害物质的名称及含量Name and Concentration ofHazardous Substances

产品中有害物质的名称及含量Table of Hazardous Substances’ Name and Concentration

部件名称Compo-nent name

有害物质Hazardous substance

铅(Pb)

汞(Hg)

镉(Cd)

六价铬(Cr(VI))

多溴联苯(PBB)

多溴二苯醚(PBDE)

29027743 X 0 0 0 0 0

29011362 X 0 0 0 0 0

0: 表示该有害物质在该部件所有均质材料中的含量均在 GB/T 26572 规定的限量要求以下。

X: 表示该有害物质至少在该部件的某一均质材料中的含量超出 GB/T 26572规定的限量要求。

• 此表所列数据为发布时所能获得的最佳信息.

0: Indicates that this hazardous substance contained in all of the homogeneousmaterials for this part is below the limit requirement in GB/T 26572.

X: Indicates that this hazardous substance contained in at least one of thehomogeneous materials used for this part is above the limit requirement inGB/T 26572

• Data listed in the table represents best information available at the time ofpublication.


7.2.6 Declaration of Hazardous Substances (DoHS)


Index

C

Cassettes, 26CE, 84

conformity, 84marking, 84

D

Delay volume, 57set, 57

Dimensions, 44instrument, 44

E

Emergency procedures, 17emergency shutdown, 17

F

FCC compliance, 87Fraction collector, 76

cleaning, 76Fraction collector F9-C, 26, 28, 30,36, 53

Cassettes and racks, 26connect tubing, 30, 53requirements on deep wellplates, 28requirements on tubes, 28Tubes, 36

Fraction collector F9-R, 38, 53, 68,71, 78

cleaning, 78connect tubing, 38, 53Delivery arm, 71Insert collection tubes, 71Tube rack, 68

Fractionation, 37assemble tube rack, 37

I

Important user information, 6Installation, 59

performance test, 59

Instrument overview, 34Fraction collector F9-R, 34

L

Labels, 15Fraction collector F9-Cinstrument label, 15

M

Manufacturing information, 83

N

Notes and tips, 7

P

Prepare the system, 55waste tubing, 55

Prerequisites, 6Purpose of this manual, 5

R

Racks, 26Recycling information, 81

decontamination, 81Regulatory information, 82

S

Safety notices, 6Safety precautions, 9, 17

emergency procedures, 17Storage, 41

T

Tube rack, 37assembly, 37

Typographical conventions, 5

W

Waste tubing, 55prepare, 55

Index


Weight, 43instrument, 43

Index


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