for research use only ikb alpha polyclonal antibody … · 2020-07-28 · ikb alpha polyclonal...
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IkB Alpha Polyclonal antibodyCatalog Number:10268-1-AP 151 Publications
For Research Use Only
www.ptglab.com
Basic Information Catalog Number:10268-1-AP
Size:150UL , Concentration: 160 μg/ml byBradford method using BSA as thestandard;
Source:Rabbit
Isotype:IgG
Immunogen Catalog Number:AG0345
GenBank Accession Number:BC002601
GeneID (NCBI):4792
Full Name:nuclear factor of kappa lightpolypeptide gene enhancer in B-cellsinhibitor, alpha
Calculated MW:36 kDa
Observed MW:36 kDa
Purification Method:Antigen affinity purification
Recommended Dilutions:WB 1:1000-1:4000 IP 0.5-4.0 ug for IP and 1:500-1:2000for WBIHC 1:50-1:500 IF 1:50-1:500
Applications Tested Applications:FC, IF, IHC, IP, WB, ELISA
Cited Applications:FC, IF, IHC, WB
Species Specificity:human, mouse, pig
Cited Species:A. sphaerocephala Krasch, broiler chick, CHICKEN,human, mouse, rat, swine
Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0
Positive Controls:
WB : HeLa cells, A431 cells, HEK-293 cells, HL-60 cells,Jurkat cells, pig liver tissue, HepG2 cells, NIH/3T3cells, mouse liver tissues
IP : HeLa cells,
IHC : human kidney tissue, mouse kidney tissue,human stomach tissue, human prostate cancer tissue
IF : HeLa cells,
Background Information Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3,NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited byI-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm.Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destructionvia the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complextranslocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
Notable Publications Author Pubmed ID Journal Application
Yuqian Wang 32942847 J Agric Food Chem WB
Longlong Li 32940033 J Agric Food Chem WB
Wei Bao 33052237 Theranostics WB
Storage Storage:Store at -20°C. Stable for one year after shipment.Storage Buffer:PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Aliquoting is unnecessary for -20ºC storage
T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)
E: [email protected] W: ptglab.com
For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.
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Selected Validation Data
Various lysates were subjected to SDS PAGEfollowed by western blot with 10268-1-AP (IkBalpha antibody) at dilution of 1:2000 incubated atroom temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under10x lens).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under40x lens).
Immunofluorescent analysis of (-20 Ethanol) fixedHeLa cells using 10268-1-AP (IkB alpha antibody)at dilution of 1:50 and Alexa Fluor 488-conjugatedAffiniPure Goat Anti-Rabbit IgG(H+L).
IP Result of anti-IkB alpha (IP:10268-1-AP, 4ug;Detection:10268-1-AP 1:1000) with HeLa cellslysate 4000ug.
1X10^6 HeLa cells were stained with 0.2ug IkBalpha antibody (10268-1-AP, red) and controlantibody (blue). Fixed with 90% MeOH blockedwith 3% BSA (30 min). Alexa Fluor 488-conjugatedAffiniPure Goat Anti-Rabbit IgG(H+L) with dilution1:1000.