food safety / analytic kits · fis-e01 fish (parvalbumin) 270 glu-e02 gluten / gliadin 270 haz-e01...

Food Safety / Analytic Kits (Price List 2014 • Distributors)

Immunolab

of 2

Immunolab GmbH fon +49 (0)561 491 742-0 mail [email protected] Otto-Hahn-Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742-20 web www.immunolab.de

PREL-FA_dis_eng_v3

Food ELISA (96 Tests)

Cat. No. Product Name Price (EUR) 1

Mycotoxins

AB1-E01 Aflatoxin B1 195

AM1-E01 Aflatoxin M1 250

DON-E01 Deoxynivalenol (DON) 230

DON-E03 Deoxynivalenol (DON) RAPID 230

FUM-E01 Fumonisin 230

FUM-E03 Fumonisin RAPID 230

OTA-E01 * Ochratoxin A 230

ZEA-E01 Zearalenone 230

ZEA-E03 Zearalenone RAPID 230

T2T-E01 T2-Toxin 230

Vitamins

FOL-E01 Folic Acid 270

B12-E01 Vitamin B12 270

BIO-E01 Vitamin H (Biotin) 270

Antibiotics

CAP-E02 Chloramphenicol 230

PEN-E01 Penicillin 250

STR-E02 Streptomycin 250

TCY-E01 * Tetracycline upon request

Hormones

EST-E01 Estradiol 195

PRO-E01 Progesterone 195

TES-E01 Testosterone 195

Miscellaneous

HIS-E02 Histamine 270

HIS-E03 Histamine RAPID 270

* in development

Food Safety / Analytic Kits (Price List 2014 • Distributors)

Immunolab

of 2


PREL-FA_dis_eng_v3

Food ELISA (96 Tests) continuation


Allergens

ALM-E01 Almond 270

BLG-E01 Beta-Lactoglobulin 270

CAS-E01 Casein 270

CAW-E01 Cashew 270

CEL-E01 * Celery 270

CRU-E01 Crustaceans (Tropomyosin) 270

EGG-E01 Egg White 270

FIS-E01 Fish (Parvalbumin) 270

GLU-E02 Gluten / Gliadin 270

HAZ-E01 Hazelnut 270

LUP-E01 Lupine 270

LYS-E01 Lysozyme 270

MIL-E01 Milk 270

MUS-E01 Mustard 270

OVA-E01 Ovalbumin 270

PEA-E01 Peanut 270

PEC-E01* Pecan Nut 270

PIS-E01 Pistachio 270

SES-E01 Sesame 270

SOJ-E01 Soja (Soy) 270

WAL-E01 Walnut 270

* in development

Food Immunoaffinity Columns (10 columns / kit)


AFB-A01 Aflatoxin (total) 65

AB1-A01 Aflatoxin B1 65

B12-A01 Vitamin B12 95

BIO-A01 Vitamin H (Biotin) 95

ZEA-A01 Zearalenone 80

1 All prices are given in EURO, net ex works, exclusive of packaging.

Food Intolerance ELISA Tests (Price List 2014 • Distributors)

Immunolab


PREL-FI_dis_eng_v2


Food IgG ELISA 2

ILE-SCG25 Specific IgG Screening, Nutritional, 3x 24 food antigens 80

ILE-SCG26 Specific IgG MultiScreen, Nutritional, 1x 88 food antigens 80

Food IgG4 ELISA 2

ILE-SCG06 Specific IgG4 Screening, Nutritional, 4x 20 food antigens 80

ILE-SCG80 Specific IgG4 MultiScreen, Nutritional, 1x 88 food antigens 80

ILE-SCG210 Specific IgG4 Screening, Nutritional, 3x 24 food antigens 80

Coeliacal Antibodies

ILE-GLI01 Gliadin / Gluten IgG, 96 tests 90

ILE-GLI02 Gliadin / Gluten IgA, 96 tests 100

ILE-GLI03 Gliadin / Gluten IgM, 96 tests 100

1 All prices are given in EURO, net ex works, exclusive of packaging.

2 For Food IgG and IgG4 various formats (number of food antigens per microtiter plate) and

invidual, regional specific food antigen compositions can be ordered.

Infectious Disease ELISA Kits (96 Tests) (Price List 2014 • Distributors)

Immunolab

Page 1 of 3


PREL-IN_dis_eng_v3

Cat. No. Product Name Price (EUR) *

ILE-ADV01 Adenovirus IgG 90

ILE-ADV02 Adenovirus IgA 100

ILE-ADV03 Adenovirus IgM 100

ILE-ASP01 Aspergillus fumigatus IgG 80

ILE-ASP02 Aspergillus fumigatus IgA 90

ILE-ASP03 Aspergillus fumigatus IgM 90

ILE-BPT01 Bordetella pertussis IgG 80

ILE-BPT02 Bordetella pertussis IgA 90

ILE-BPT03 Bordetella pertussis IgM 90

ILE-BPT04 Bordetella pertussis Toxin IgG 110

ILE-BPT07 Bordetella pertussis FHA IgG 110

ILE-BOR01 Borrelia burgdorferi IgG 90

ILE-BOR03 Borrelia burgdorferi IgM 100

ILE-BRU01 Brucella abortus IgG 90

ILE-BRU02 Brucella abortus IgA 100

ILE-BRU03 Brucella abortus IgM 100

ILE-CAN01 Candida albicans IgG 80

ILE-CAN02 Candida albicans IgA 90

ILE-CAN03 Candida albicans IgM 90

ILE-CMV01 Cytomegaly IgG 60

ILE-CMV03 Cytomegaly IgM 70

ILE-DIP01 Diphtheria IgG (Corynebacterium diphtheriae) 100

ILE-EBA01 Epstein-Barr Early Antigen (EA) IgG 90

ILE-EBA02 Epstein-Barr Early Antigen (EA) IgA 100

ILE-EBA03 Epstein-Barr Early Antigen (EA) IgM 100

ILE-EBV01 Epstein-Barr Nuclear Antigen (EBNA) IgG 90

ILE-EBV02 Epstein-Barr Nuclear Antigen (EBNA) IgA 100

ILE-EBV03 Epstein-Barr Nuclear Antigen (EBNA) IgM 100

ILE-EBC01 Epstein-Barr Virus Capsid Antigen (VCA) IgG 90

ILE-EBC02 Epstein-Barr Virus Capsid Antigen (VCA) IgA 100

ILE-EBC03 Epstein-Barr Virus Capsid Antigen (VCA) IgM 100


Immunolab

Page 2 of 3


PREL-IN_dis_eng_v3

Cat. No. Product Name Price (EUR)

ILE-ECH01 Echinococcus granulosus IgG 90

ILE-FSM01 TBEV (FSME) IgG 100

ILE-FSM03 TBEV (FSME) IgM 110

ILE-HEL01 Helicobacter pylori IgG 80

ILE-HEL02 Helicobacter pylori IgA 90

ILE-HEL03 Helicobacter pylori IgM 90

ILE-HS101 Herpes 1 IgG 80

ILE-HS102 Herpes 1 IgA 90

ILE-HS103 Herpes 1 IgM 90

ILE-HS201 Herpes 2 IgG 80

ILE-HS202 Herpes 2 IgA 90

ILE-HS203 Herpes 2 IgM 90

ILE-HSC01 Herpes 1&2 IgG 80

ILE-HSC02 Herpes 1&2 IgA 90

ILE-HSC03 Herpes 1&2 IgM 90

ILE-IFA01 Influenza A IgG 80

ILE-IFA02 Influenza A IgA 90

ILE-IFA03 Influenza A IgM 90

ILE-IFB01 Influenza B IgG 80

ILE-IFB02 Influenza B IgA 90

ILE-IFB03 Influenza B IgM 90

ILE-MAS01 Measles IgG 80

ILE-MAS02 Measles IgA 90

ILE-MAS03 Measles IgM 90

ILE-MJS01 MeljaSorb (RF Absorbent) 40

ILE-MUM01 Mumps IgG 80

ILE-MUM02 Mumps IgA 90

ILE-MUM03 Mumps IgM 90


Immunolab

Page 3 of 3


PREL-IN_dis_eng_v3

Cat. No. Product Name Price (EUR)

ILE-MYK01 Mycoplasma pneumoniae IgG 90

ILE-MYK02 Mycoplasma pneumoniae IgA 100

ILE-MYK03 Mycoplasma pneumoniae IgM 100

ILE-PAX01 Parainfluenza 1, 2 & 3 IgG 80

ILE-PAX02 Parainfluenza 1, 2 & 3 IgA 90

ILE-PAX03 Parainfluenza 1, 2 & 3 IgM 90

ILE-POL01 Poliomyelitis IgG 110

ILE-RSV01 Respiratory syncytial virus (RSV) IgG 90

ILE-RSV02 Respiratory syncytial virus (RSV) IgA 100

ILE-RSV03 Respiratory syncytial virus (RSV) IgM 100

ILE-RUB01 Rubella IgG 60

ILE-RUB03 Rubella IgM 70

ILE-TET01 Tetanus IgG (Clostridium tetani) 100

ILE-TOX01 Toxoplasma IgG 60

ILE-TOX03 Toxoplasma IgM 70

ILE-TUB01 Tuberculosis IgG (Mycobacterium tuberculosis) 120

ILE-TUB02 Tuberculosis IgA (Mycobacterium tuberculosis) 140

ILE-TUB03 Tuberculosis IgM (Mycobacterium tuberculosis) 140

ILE-TUS01 Tuberculosis IgG sensitive (Mycobacterium tub.) 120

ILE-TUS02 Tuberculosis IgA sensitive (Mycobacterium tub.) 140

ILE-TUS03 Tuberculosis IgM sensitive (Mycobacterium tub.) 140

ILE-VAR01 Varicella zoster IgG 80

ILE-VAR02 Varicella zoster IgA 90

ILE-VAR03 Varicella zoster IgM 90

ILE-YER01 Yersinia enterocolitica IgG 80

ILE-YER02 Yersinia enterocolitica IgA 90

ILE-YER03 Yersinia enterocolitica IgM 90

* All prices are given in EURO, net ex works, exclusive of packaging.

Food Safety / Analytic Kits

Newsletter Allergen ELISA Kits Immunolab

Immunolab GmbH fon +49 (0)561 491 742‐0 mail [email protected] Otto‐Hahn‐Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742‐20 web www.immunolab.de

PRIN-FA_allergens_eng_v5

Food Allergy is a rapidly increasing area of consumer concern. Food allergens are proteins in food that, even in low concentrations, can trigger allergic, sometimes life‐threatening reactions in sensitive individuals.

As protection of the consumer, the EU directive 2003/89/EC was issued in November 2005. It obliges the food manufacturers to label their products with all ingredients capable of causing an allergic reaction. Annex IIIa includes a list of food ingredients classified as being possible allergens or intolerants, e.g. peanuts, hazelnuts, eggs, gluten, almond, milk, soy, crustaceans and celery.

Testing for the presence of food residues provides security for food manufacturers that a potentially dangerous ingredient is not included in their food products.

Immunolab GmbH as an acknowledged developer and manufacturer of high‐quality Food Safety/Analytic and Clinical Diagnostic products, hereby introduces its new food allergen product line to detect almond, beta‐lactoglobulin, casein, crustaceans, egg white, fish, gluten, hazelnut, lupine, mustard, peanut, soy and walnut residues in raw materials and processed foods. Further parameters (Celery, Fish) are in development. The Immunolab food allergen kits are 96 well ELISA tests and can be processed according to a usual standard sandwich ELISA protocol. Each kit includes all components required to perform the assay. Features and Benefits • Easy Handling by:

o Standardized Test Protocol o Short Assay Time, 60 minutes (20/20/20 min) o Reagents (excl. wash buffer) ready‐to‐use; breakable strips o Simple extraction procedure and no further clean‐up steps o Incubation at ambient temperature

• High Accuracy and Reproducibility • High Stability, minimum shelf life 12 months

Technical Performance Data

Analyte LOD LOQ Ref. Range Recovery Precision (intra‐assay)

Almond 0.2 ppm 0.4 ppm 0 – 10 ppm 67 – 109 % 3 – 9 %

Beta‐Lactoglobulin 1.5 ppb 10 ppb 0 – 400 ppb 70 – 107 % 7 %

Casein 0.04 ppm 0.2 ppm 0 – 6 ppm 80 – 102 % 5 ‐ 11 %

Celery * available soon available soon available soon available soon available soon

Crustaceans (Tropomyosin) 0.9 ppb 20 ppb 0 – 400 ppb 84 – 97 % 6 – 8 %

Egg White 0.05 ppm 0.4 ppm 0 – 10 ppm 82 – 98 % 4 – 9 %

Fish (Parvalbumin) * available soon available soon available soon available soon available soon

Gluten / Gliadin 0.3 ppm 2 ppm 0 – 60 ppm 85 – 98 % 4 – 5 %

Hazelnut 0.3 ppm 1 ppm 0 – 40 ppm 83 – 101 % 7 – 12 %

Lupine 0.2 ppm 2 ppm 0 – 30 ppm 98 – 113 % 6 – 9 %

Mustard 1 ppm 2 ppm 0 – 60 ppm 76 – 98 % 8 %

Peanut 0.1 ppm 1 ppm 0 – 40 ppm 90 – 110 % 7 – 10 %

Sesame 0.2 ppm 2 ppm 0 – 30 ppm 85 – 110 % 5 – 12 %

Soja (Soy) 16 ppb STI 40 ppb STI 0 –1000 ppb 77 – 122 % 6 – 8 %

Walnut 0.35 ppm 2 ppm 0 – 60 ppm 75 – 106 % 4 – 9 %

Food Safety / Analytic ELISA Mycotoxins Cat. No. Aflatoxin B1 AB1‐E01 Aflatoxin M1 AM1‐E01 Deoxynivalenol (DON) * DON‐E01 Fumonisin FUM‐E01 Ochratoxin A * OTA‐E01 Zearalenone ZEA‐E01

Antibiotics Chloramphenicol CAP‐E02 Penicillin PEN‐E01 Streptomycin STR‐E02 Tetracycline * TCY‐E01

Vitamins Folic Acid (Vit. B9) FOL‐E01 Vitamin B12 B12‐E01 Biotin (Vitamin H) BIO‐E01

Hormones Estradiol EST‐E01 Progesterone PRO‐E01 Testosterone TES‐E01

Miscellaneous Histamine HIS‐E02 Gluten / Gliadin GLU‐E01

Allergens Almond ALM‐E01 Beta‐Lactoglobulin BLG‐E01 Casein CAS‐E01 Celery * CEL‐E01 Crustaceans CRU‐E01 Egg white EGG‐E01 Fish (Parvalbumin) * FIS‐E01 Gluten / Gliadin GLU‐E02 Hazelnut HAZ‐E01 Lupine LUP‐E01 Mustard MUS‐E01 Peanut PEA‐E01 Sesame SES‐E01 Soja (Soy) SOJ‐E01 Walnut WAL‐E01

* in development

Food Intolerance Tests » For a Better Quality of Life « Immunolab Food Antigens

page 1 of 2 PRIN-FI antigens_eng_v3 [Revision: June 2011]

Immunolab GmbH fon +49 (0)561 491 742‐0 mail [email protected]

Otto‐Hahn‐Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742‐20 web www.immunolab.de

agar

agave

alfalfa

allspice

almond

aloe vera

amaranth

anchovy

angler

anise

appenzeller

apple

apricot

artichoke

asparagus

avocado

baker's yeast

baking powder

bamboo

banana

barley

basil

batata

bay leaf

bear's garlic

beef

beetroot

bell pepper

black currant

black eyed pea

black radish

black salsify

blackberry

blue mold cheese

blue mussel

blueberry

borage

brazil nut

brewer's yeast

broad bean

broccoflower

broccoli

brook trout

brussels sprout

buck's horn plantain

buckwheat

butter

buttermilk

button mushroom

calf's liver

camel milk

camembert

cantaloupe, north amer.

canderel

cape gooseberry

caper

carambola

caraway

cardamom

carob

carp

carrageenan

carrot

casein

cashew nut

cauliflower

caviar black

caviar red

cayenne pepper

celeriac

celery

cep

chamomile

chanterelle

chard

cheddar

cherimoya

cherry

chervil

chestnut

chick pea

chicken

chicory

chili

chinese cabbage

chive

chlorella

cinnamon

clementine

clove

coalfish

cocoa

coconut

coconut milk

cod

coffee

coke

common shrimp

coriander

corn salad

cottage cheese

courgette

cowberry

cow's milk

cranberry

crayfish

cream

cream cheese

cucumber

cumin

curd

curry

cuttlefish

damson

dandelion

dark chocolate

date

desert indianwheat

dill

duck

duck egg

east african land snail

edam

eddo

edible snail

eel

egg replacement powder

egg white

egg yolk

eggplant

elderberry

emmental

emmer

endive

European cantaloupe

european perch

fallow deer

fennel

fig

flax seed

flounder

garden cress

garlic

gelatin

gilthead seabream

gin

ginger

gliadin

glutamate

goat

goat cheese

goat's milk

golden syrup

goose

gooseberry

goose egg

gouda

granadilla

grape red

grape white

grapefruit

graviera

gruyere cheese

guar gum

guava

gum arabic

gum karaya

haddock

hake

halibut

halloumi

hare

haw

hawthorn

hazelnut

hemp flour

herring

honey acacia

honey clover

honey rape

honeydew melon

hop

horse

horseradish

horta

hot pepper green

hot pepper red

iceberg lettuce

indian fig

iridescent shark‐catfish

jenever

jerusalem artichoke

juniper berry

kaki

kale

kamut

kapuisto tiri

kaseri

kefalotyrh

kefir

kiwano

kiwi

kolanut

kumquat

α‐lactalbumin

lactoferrin

β‐lactoglobulin

lamb

lavender

lecithin

leek

leerdam cheese

lemon

lemon balm

lemon soda

lemon verbena

lemongrass

lentil

lettuce

lime

lime blossom

liquorice

lobster

locust bean gum

lollo rosso

Food Intolerance Tests » For a Better Quality of Life « Immunolab Food Antigens

page 2 of 2 PRIN-FI antigens_eng_v3 [Revision: June 2011]


Otto‐Hahn‐Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742‐20 web www.immunolab.de

lovage

lukumia

lupin

lychee

lysozyme

macadamianut

mackerel

maize

maize meal

mallow

malt

mandarin

mangelwurzel

mango

mangosteen

manioc

maple syrup

mare's milk

marigold

marjoram

marzipan

mate

milk chocolate

millet

mirabelle plum

mistletoe leave

mistletoeberry

morel

mozzarella

mugwort

mung bean

mustard seed

mustard, brown

mustard, green

mustard, white

nashi

nectarine

nile perch

nile tilapia

noni

nori

nutmeg

oat

octopus

okra

olive black

olive green

olive oil

onion

orange

orange soda

oreagno

ostrich

ovalbumin

ovomucoid

ovotransferrin

oyster

oyster mushroom

palm heart

palm oil

papaya

paprika

parmesan

parsley

parsley root

parsnip

passion fruit

pastirma

pea

peach

peanut

pear

pecan nut

pectin

pepino

pepper black

pepper green

pepper white

peppermint

pheasant

pigeon

pike

pine nut

pineapple

pinto bean

pistachio

pitaya yellow

plaice

plantain

plum

pointed cabbage

pomegranate

pomelo

poppy seed

pork

potato

prawn

processed cheese

pumpkin

pumpkin seed

quail

quail egg

quince

quinoa

rabbit

radicchio

raisin

rape seed

raspberry

red bean

red cabbage

red currant

red deer

red mullet

red radish

red snapper

red tea

reindeer

retsina

rhubarb

rice

ricotta

rocket

romaine lettuce

roquefort

rosefish

roselle

rosemary

rum

rye

safflower oil

saffron

sage

Saint John's wort

salmon

sardine

sauerkraut

savory

savoy cabbage

scallop

sea bass

sea lamprey

seabuckthornberry

semolina

sesame

shallot

sheathed woodtuft

sheep's milk

sheep's milk cheese

shiitake

shrimps

sloe

snap pea

sole

sorrel

sour cherry

soy grain

soybean

soymilk

spelt

spinach

spined loach

spiny lobster

spirulina

squid

starch

sticky bun

stinging nettle

strawberry

string bean

sugar beet molasses

sultana

sunflower seed

swedish turnip

swordfish

tapioca

tarragon

tea black

tea green

tea white

teff

thyme

tiger nut

tilsit cheese

tobacco

tofu

tomato

tragacanth

triticale

trout

tuna

turbot

turkey

turmeric

turnip cabbage

turshi

unripe spelt grains

valerian

vanilla

veal

venison

venus clam

wakame

walnut

watermelon

wels catfish

wheat

whey

white bean

white cabbage

white chocolate

whole egg

wild boar

wild rice

wine leave

wine red

wine white

woodruff

wormwood

yam

yellow bean

yogurt

xanthan gum

zander

Food Intolerance Tests » For a Better Quality of Life «

Relevance of the detection of specific IgG / IgG4 Immunolab antibodies in the diagnosis of food intolerances

Page 1


PRIN-FI_background_eng_v1

There is increased clear epidemiological evidence during recent years that more and more people suffer from food intolerance symptoms. An analogous tendency is also observed for classical allergic diseases (IgE mediated, type 1, immediate reaction). The use of IgG and/or IgG4 tests to detect the triggering food antigens is still a controversially discussed subject among medical experts. Despite this situation the detection of IgG/IgG4 antibodies should always be considered if typical causes can be excluded like lactose or fructose malabsorption, celiac disease or an IgE mediated food allergy (type 1).

The allergic immediate reaction (type 1) is associated with a Th2 cell activation. Resulting from both the allergen portal of entry and exposure time the Th2 activation can switch to a more Th1 directed reaction leading to the formation of IgG4 and IgA antibodies. If this effect increases further towards Th1, IgG1 antibodies can be produced.

Specific IgG antibodies can be found in the serum of food allergy patients as well. In fact, numerous studies have described higher IgG4 levels in patients with IgE mediated symptoms (type 1) compared to non allergic individuals. The extent of the allergen exposure and exposure time have a strong impact on the concentration of IgG4 antibodies. This leads to the conclusion that an IgG4 reaction cannot be regarded as a physiological reaction only but has a pathological significance from a certain reactivity level.

There are four IgG subclasses of which IgG1 and IgG4 play a particular role in food intolerances. IgG1 is produced as initial response after contact with a new food antigen. After further exposure to the antigen a switch occurs towards formation of IgG4 antibodies

(1).

The IgG4 portion of total IgG can increase from 3‐5% to 50%. The elevated IgG4 concentration points to an intense conflict between the immune system and the triggering food antigen. Functionally, clear evidence could be obtained that IgG4 antibodies are able to sensitize cells temporarily but can block the allergic response as well. In fact 50% of untreated allergic patients show elevated IgG4 levels. Often disorders appear after hours only, causing difficulties to identify the triggering food. In many cases a disturbed intestinal permeability is postulated to initiate the food intolerance. This leads to a permanent exposure of the immune system by antigenic structures. The overload of the immune system can result in an enhanced sensitivity to infections.

Regarding the diagnostic relevance of IgE‐ and IgG/IgG4 reactions it should be considered that immunological mechanisms and related symptoms can be different in inhalation allergies and food intolerances. In particular a non‐IgE mediated reaction is known for food intolerances for a long time.



Page 2



The critics of IgG/IgG4 tests, mainly allergologists and their professional associations, persistently complain about the lack of scientific studies, without presenting concrete own data though. Indeed numerous articles have been published during recent years which support the relevance of the IgG/IgG4 antibodies’ detection for the diagnosis of food intolerances. The conclusion of some of these publications are presented as follows:

A Norwegian group postulated an important immunopathological role of specific IgG4 antibodies in patients with a delayed non‐IgE mediated cow’s milk allergy. This patient group showed significantly higher concentrations of ß‐lactoglobulin (ß‐LG)‐ specific IgG4 antibodies compared to cow’s milk tolerized patients. (2)

A food intolerance reflected by IgG antibodies can be associated with an increased risk of arteriosclerosis and low grade inflammation (elevated CRP levels) in obese juveniles. (3)

Other publications support the high significance of IgG4 tests to determine and monitor an elimination diet in patients with an irritable bowel syndrome. Moreover, the investigations of Zar point to a positive impact on psychic symptoms, based on IgG4 tests.

(4‐7)

Bernardi has found highly significant differences of IgG4 titers in patients with food intolerance to 19 food antigens compared to asymptomatic healthy subjects. The obtained sensitivity and specificity were 81% and 87%, respectively. Based on the described test results exclusion diets were performed leading to a significant improvement of the symptoms in 78.5% of all cases after 2 months only. An IgG4 control testing after this time period proved a manifest reduction of the previously elevated IgG4 titers.

(8)

Even in migraine patients symptoms could be improved by exclusion diets that were based on IgG results. (9)

A less recent publication describes the detection of high IgG4 concentrations in patients with confirmed sensitization to soy beans. In contrast low reactivity was found for IgE and IgG. (10)

In another investigation of patients showing asthma symptoms after contact with soy dust authors found that both specific IgE and IgG4 antibody levels to soy were increased. They postulated that the IgG4 increase is part of the pathological asthma process. (11)



Page 3



References

1. Snapper et al. (1999) in Fundamental Immunology, ed. Paul, W. E. (Lippincott, Philadelphia), pp. 831–861.

2. Sletten et al. (2006). Changes in humoral responses to beta‐lactoglobulin in tolerant patients suggest a particular role for IgG4 in delayed, non‐IgE‐mediated cow's milk allergy. Pediatr. Allergy Immunol. 17, 435‐443.

3. Wilders‐Truschnig, M. et al. (2008). IgG Antibodies against Food Antigens are Correlated with Inflammation and Intima Media Thickness in Obese Juveniles. Exp Clin Endocrinol Diabetes 116(4):241‐5.

4. Atkinson et al. (2004). Food elimination based on IgG antibodies in irritable bowel syndrome, a randomised controlled trial. Gut 53:1459–1464.

5. Zar et al. (2005). Food‐specific IgG4 antibody‐guided exclusion diet improves symptoms and rectal compliance in irritable bowel syndrome. Scand J. Gastroenetrol. 40, 800‐807.

6. Zar et al. (2005). Food‐Specific Serum IgG4 and IgE Titers to Common Food Antigens in Irritable Bowel Syndrome. Amer. J. Gastroenetrol. 100, 1550‐1557.

7. Drisko et al. (2006). Treating irritable bowel syndrome with a food elimination diet followed by food challenge and probiotics. J. Amer. College Nutrition 25(6), 514‐522.

8. Bernardi et al. (2008). Time to reconsider the clinical value of immunoglobulin G4 to foods. Clin. Chem. Lab. Med. 46, 687‐690.

9. Hernandez et. al. (2007). Food allergy mediated by IgG antibodies associated with migraine in adults. Rev Alerg Mex 54(5), 162‐168.

10. Awazuhara et al. (1997). Major allergens in sojabean and clinical significance of IgG4 antibodies investigated by IgG and IgG4 immunoblotting with sera from sojabean sensitive patients.Clin. Exp. Allergy 27, 325‐332.

11. Codina et al. (1997). Specific immunoglobulins to soybean hull allergens in soybean asthma, Chest 111, 75‐80.

Infectious Disease ELISA » We Protect Your Health «

Immunolab

Immunolab GmbH, an acknowledged developer and manufacturer of high‐quality immunodiagnostic and analytical reagents, provides you with a wide range of enzyme immunoassays for the detection of infectious disease parameters. Our product line is suitable for routine applications as well as research purposes.

The Immunolab Infectious Disease Tests are microtiter plate based enzyme immunoassays (ELISA) for the qualitatitive and quantitative determination of IgA, IgG and IgM antibodies to multiple infectious agents in human sera and plasma. Further applications in other body fluids are possible.

Each kit includes all ingredients required to perform the assay. One kit provides 96 tests. All reagents are ready‐to‐use. Assay Performance Scheme:

Otto‐Hahn‐Straße 16, 34123 Kassel, Germany fax +49 (0)561 491 742‐20 web www.immunolab.de PRIN-IN_general info_eng_v2

Features and Benefits

Broad Panel including TORCH

IgG, IgA and IgM detection for most parameters

Short Assay Time

Easy Handling by:

o Uniform Test Format

o Ready‐to‐use and interchangeable Reagents

o Breakable Strips

o Standardized Performance (identical volumes, dilution)

o Identical Incubation Times for all parameters

o Incubation at ambient temperature

Wide Reference Range by 4 point calibration

Qualitatitive (using cut‐off) and/or Quantitative (using calibrators) Test Results

Quantitative Result with just one sample dilution

High Stability, minimum shelf life 12 months

Registered in the European Union according to IVDD 98/79/EC

Immunolab Infectious Disease ELISA

Adenovirus (IgA, IgG, IgM) Aspergillus fumigatus (IgA, IgG, IgM) Bordetella pertussis (IgA, IgG, IgM) Bordetella pertussis Toxin (IgG) Bordetella FHA (IgG) Borrelia burgdorferi (IgG, IgM) Brucella abortus (IgA, IgG, IgM) Candida albicans (IgA, IgG, IgM) Cytomegaly Virus (IgG, IgM) Diphtheria (IgG) Echinococcus granulosus (IgG) Epstein‐Barr‐Virus EA (IgA, IgG, IgM) Epstein‐Barr‐Virus EBNA (IgA, IgG, IgM) Epstein‐Barr‐Virus VCA (IgA, IgG, IgM) Helicobacter pylori (IgA, IgG, IgM) Herpes simplex (HSV) 1 (IgA, IgG, IgM) Herpes simplex (HSV) 2 (IgA, IgG, IgM) Herpes simplex (HSV) 1/2 (IgA, IgG, IgM) Influenza A (IgA, IgG, IgM) Influenza B (IgA, IgG, IgM) Measles (IgA, IgG, IgM) Mumps (IgA, IgG, IgM) Mycobact. tuberculosis (IgA, IgG, IgM) Mycoplasma pneumoniae (IgA, IgG, IgM) Parainfluenza 1/2/3 (IgA, IgG, IgM) Resp. Syn. Virus (RSV) (IgA, IgG, IgM) Poliomyelitis (IgG) Rubella (IgG, IgM) TBE Virus (FSME) (IgG, IgM) Tetanus (IgG) Toxoplasma (IgG, IgM) Varicella zoster (IgA, IgG, IgM) Yersinia enterocolitica (IgA, IgG, IgM)

Probe (1:100)

Antigen beschichtete Mikrotiter-Kavität

E

Spezifische Antikörper binden an das Antigen

Sekundärantikörper (Konjugat) bindet an die Fc-Region des spezifischen Antikörpers

60 Min, RT

HRP (E) Enzymaktivität wandelt das farblose- in blaues TMB um.

Messung Konjugat (HRP)

30 Min, RT

Substrat (TMB)

20 min, RT

StopplösungE E E E E

450 nm

OD

Testresultate

Konz.

Stopplösung beendet die Enzymreaktion und führt zu einem gelben Farbumschlag


Infectious Disease ELISA – TORCH » We Protect Your Health « Immunolab


PRIN-IN_torch_eng_v1

TORCH is an acronym for a group of infectious diseases showing an increased prevalence to cause illness in pregnant women and birth defects in their newborns. The TORCH panel consists of the following tests:

Toxoplasma gondii, Rubella virus, Cytomegalovirus (CMV) and Herpes simplex virus type 1 + 2.

Immunolab GmbH, an acknowledged developer and manufacturer of high‐quality immunodiagnostic and analytical reagents provides you with the complete TORCH panel. Our highly sensitive and specific Toxoplasma, Rubella, Cytomegaly and Herpes simplex kits are in compliance with the European in‐vitro diagnostic directive (IVDD) 98/79/EC and as such are CE marked. The Immunolab TORCH tests are microtiter plate based enzyme immunoassays (ELISA) for the qualitatitive and quantitative determination of IgG, IgM and IgA (only HSV) antibodies to Toxoplasma gondii, Rubella, Cytomegalovirus (CMV) and Herpes simplex virus in human sera and plasma.

Each kit includes all components required to perform the assay. One kit provides 96 tests. All reagents are ready‐to‐use. Assay Performance Scheme:

Features and Benefits

• Short Assay Time

• Easy Handling by:

o Uniform Test Format

o Ready‐to‐use and interchangeable Reagents

o Breakable Strips

o Standardized Performance (identical volumes, incubation times, dilutions)

o Incubation at ambient temperature

• High Stability, minimum shelf life 12 months

• Test results are qualitatitive (using cut‐off) and/or quantitative (using calibrators)

• Quantitative Result with just one sample dilution

• Reference curve calibrated against International WHO Reference Preparations: o Rubella IgG, 1st Intl. Standard RUBI‐1‐94. o Toxoplasma IgG, standard TOXM (3rd Intl. Standard)

• Product Registration acc. to the Europ. in vitro diagnostic directive IVDD 98/79/EC

Immunolab Infectious Disease ELISA Adenovirus (IgA, IgG, IgM) Aspergillus fumigatus (IgA, IgG, IgM) Bordetella pertussis (IgA, IgG, IgM) Borrelia burgdorferi (IgG, IgM) Brucella abortus (IgA, IgG, IgM) Candida albicans (IgA, IgG, IgM) *Cytomegaly Virus (IgG, IgM) Diphtheria (IgG) Echinococcus granulosus (IgG) Epstein‐Barr Virus EA (IgA, IgG, IgM) Epstein‐Barr Virus EBNA (IgA, IgG, IgM) Epstein‐Barr Virus VCA (IgA, IgG, IgM) Helicobacter pylori (IgA, IgG, IgM) *Herpes simplex 1, 2 and 1/2 (IgA, IgG, IgM) Influenza A and B (IgA, IgG, IgM) Measles (IgA, IgG, IgM) Mumps (IgA, IgG, IgM) Mycobact. tuberculosis (IgA, IgG, IgM) Mycoplasma pneumoniae (IgA, IgG, IgM) Parainfluenza 1/2/3 (IgA, IgG, IgM) Resp. Syn. Virus (RSV) (IgA, IgG, IgM) Poliomyelitis (IgG) *Rubella (IgG, IgM) TBE Virus (FSME) (IgG, IgM) Tetanus (IgG) *Toxoplasma gondii (IgG, IgM) Varicella zoster (IgA, IgG, IgM) Yersinia enterocolitica (IgA, IgG, IgM)

*TORCH Kits marked in red and bold letters

Sample (1:100)

antigen coated microtiter well

E

specific antibody binds to immobilized antigen

secondary antibody (conjugate) binds to fc of specific antibody

60 min, RT

HRP enzyme activity converts colorless TMB into blue TMB

Measurement Conjugate (HRP)

30 min, RT

Substrate (TMB)

20 min, RT

Stop Sol.E E E E E

450 nm

Test Results stop sol. terminates color development and changes color to yellow

OD

Conc.

food safety / analytic kits · fis-e01 fish (parvalbumin) 270 glu-e02 gluten / gliadin 270 haz-e01...

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