Fluorescent principle. Fluorescent microscope DAPI

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  • Slide 1
  • Fluorescent principle
  • Slide 2
  • Fluorescent microscope
  • Slide 3
  • DAPI
  • Slide 4
  • FITC
  • Slide 5
  • Alexa 488
  • Slide 6
  • Tetramethylrhodamine/TRITC
  • Slide 7
  • Rhodamin
  • Slide 8
  • Rhodamin Red-X
  • Slide 9
  • Alexa 568
  • Slide 10
  • Alexa 647
  • Slide 11
  • Texas-Red and Texas-Red-X
  • Slide 12
  • Alexa 594
  • Slide 13
  • Cy-Series
  • Slide 14
  • GFP and GFPuv
  • Slide 15
  • E-GFP's and DsRed
  • Slide 16
  • Table of Fluorescent Proteins
  • Slide 17
  • What is green fluorescent protein (GFP) ? GFP is a 238-amino acid protein originally isolated from the bioluminescent jellyfish Aequoria victoria Can be expressed in any organism without additional cofactors
  • Slide 18
  • Structurally, GFP represents a new motif which has been named the -can The can consists of 11 -strands and a central -helix Structure of GFP
  • Slide 19
  • GFP-Fluorophore Fluorescence is mediated by a fluorophore located at the center of the protein
  • Slide 20
  • The fluorophore is derived from the tripeptide -Ser 65 -Tyr 66 - Gly 67 - Formation of the fluorophore occurs in an autocatalytic manner Structure of the GFP-fluorophore
  • Slide 21
  • Light sources for Fluorescence
  • Slide 22
  • Filtersets and GFP's
  • Slide 23
  • Quantum efficiency of Chip
  • Slide 24
  • New Filterset
  • Slide 25
  • New Filterset-other flourochromes
  • Slide 26
  • Old Filtersets
  • Slide 27
  • Old filtersets- other flurochromes
  • Slide 28
  • Antibodies used for fluorescence
  • Slide 29
  • Laser and Fluorochromes