fluorescence micro-optics fluorescence detectors · fluorescence detectors: fle1100 15 - 19....

Feb. 8, 2012 1Ver. 1.0

＊Fluorescence micro-optics＊Fluorescence detectors

Nippon Sheet Glass Co., Ltd.Information and Telecommunication Device [email protected] Phone: +81-42-775-1546

mailto:[email protected]

Feb. 8, 2012 2Ver. 1.0

Contents

Global info. of Fluorescence micro-optics / detectors 3 - 4

Fluorescence micro-optics 5- Small optical probes for parallel detection 6- Small optical probes for placing in narrow space 7- Wide varieties of optical probes 8 - 10- Customization of optical probes 11- Wide varieties in wavelength 12- Pen-type optical heads 13 - 14

Fluorescence detectors: FLE1100 15 - 19Fluorescence detectors: FLE500 20 - 21

Applications of NSG’s Fluorescence micro-optics / detectors- for bio-chemistry and life-science 22- for environmental inspections 23- for quality inspections in production lines 24

The other application candidates 25 - 26

SELFOC used in this document is registered trademark of Nippon Sheet Glass Co., Ltd.R

2

Feb. 8, 2012 3Ver. 1.0

＊Fluorescence micro-optics＊Fluorescence detectorsNSG has developed various Fluorescence micro-optical heads applying muchexperience from SELFOC micro-optics. They are useful for mounting in manykinds of equipment such as real-time PCR machines, etc. and for realizing hand-held equipments. NSG also provides Fluorescence detectors as equipmentthat includes controllers and software. Both Fluorescence micro-optics andFluorescence detectors are commercially available. Please contact with us!

Feb. 8, 2012 4Ver. 1.0

Product line-up

Any component, combination, system can be chosen. Customization is available as well.4

Specimen

Fiber

Specimen

OpticalProbe

ConnectorFilter

LED PDSelfoc microlens

Filter

LED PDS microlens

Fluorescence Fluorescence microopticsmicrooptics

Fluorescence detectorsFluorescence detectors

Electronicscircuit

Software

SELFOC microlens

Feb. 8, 2012 5Ver. 1.0

Fluorescence micro-opticsNSG’s Fluorescence micro-optical heads are based on stacked planar structureof SELFOC lenses and wavelength filters that deriver small, stable, and cost-competitive features.

Fiber

Filter

LED PD

SpecimenProbe

SELFOC microlens

Feb. 8, 2012 6Ver. 1.0

Features of NSG’s Fluorescence micro-optics - 1

Small optical probes for parallel detectionSmall optical probes composed of SELFOC microlenses and fibers canbe placed close to specimen or can be applied to realize array heads.

No more scan …No more 2-D image sensors …

A 16ch probe array of 4.5mmA 16ch probe array of 4.5mm--pitch for 384ch micropitch for 384ch micro--well plates.well plates.

Feb. 8, 2012 7Ver. 1.0


Small optical probes for placing in narrow spaceSmall optical probes composed of SELFOC microlenses and fibers canbe placed close to specimen or can be applied to realize array heads.

No matter how narrow …No matter how complicated …

Feb. 8, 2012 8Ver. 1.0


Wide varieties of optical probes - 1Wide varieties of optical probes can be lined up by applyingSELFOC microlenses.

Many different eyestake the informationthat you want.Probes of working distances ofaround 1, 2, 4, and 10mm areprepared, corresponding totheir spot sizes of 0.1, 0.2, 0.4,and 1.0mm, respectively.

Workingdistance

Probe 4mm or 3.5mmin diameter

WD profile of the fluorescence signal(Sample thickness=1mm)

0.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

0 1 2 3 4 5WD (mm)

Fluo

resc

ence

sig

nal

［a.

u.］

4020

4010

4040

Feb. 8, 2012 9Ver. 1.0


Wide varieties of optical probes - 2Thinner probes of 1.25, 0.75, and 0.25mm in dia. have also been developed.

WD profile of the fluorescence signal(Sample thickness=1mm)

0.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

0.0 0.5 1.0 1.5 2.0 2.5WD (mm)

Fluo

resc

ence

sig

nal ［

a.u.

］

φ0.25

φ0.75

φ1.25

φ1.25

φ1.25

φ1.25

Not a needle, but a probe …

Feb. 8, 2012 10Ver. 1.0


Line-up of optical probes

We will also provide a custom product to meet your requirements.

No. Probe dia. Spot dia. W.D.

40100 4.0 (mm) 1.0 (mm) 10 (mm)4040 4.0 0.4 44020 4.0 0.2 24010 4.0 0.1 11210 1.25 0.5 11208 1.25 0.4 0.81206 1.25 0.3 0.60702 0.75 0.4 0.20200 0.25 0.2 0

No. Probe dia. Spot dia. W.D.

40100 4.0 (mm) 1.0 (mm) 10 (mm)4040 4.0 0.4 44020 4.0 0.2 24010 4.0 0.1 11210 1.25 0.5 11208 1.25 0.4 0.81206 1.25 0.3 0.60702 0.75 0.4 0.20200 0.25 0.2 0

Feb. 8, 2012 11Ver. 1.0


Customization of optical probesIn case you do not find a suitable product in our line-up, we will providea custom product to meet your requirements.

NSG’s vast experienceand knowledge of micro-optics provides the bestsolution.

Excitation beam profile

Excitation beam

Specimen

Z

[1]Y

X

Selfoc probe

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

0 1 2 3 4 5 6 7 8

WD (mm)

Fluo

resc

ence

sign

al ［

a.u.

］

Sample-t : 1mm(Simulation)Sample-t : 2mm(Simulation)Sample-t : 3mm(Simulation)Sample-t : 1mm(Measurement data)Sample-t : 2mm(Measurement data)Sample-t : 3mm(Measurement data)

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

WD (mm)

Fluo

resc

ence

sign

al［

a.u.

］

[1]

[2]

[3]

Three-typesof probesdesigned atWD = 3 – 4mm.Lines: estimatedDots: measured

Three-typesof probesdesigned atWD = 18 – 24mm.

Feb. 8, 2012 12Ver. 1.0

400 500 600 700 300

400

50

0

6

00

700

Exci

tatio

n (n

m)

Fluorescence (nm)


Wide varieties in wavelength Wide varieties in wavelength of both excitation and fluorescence are available by integrating SELFOCmicrolenses with small size filters.

Five standard lineups ( paintedrectangles ) and semi-customs( many other rectangles ) coverany position in the wavelengthcoordinates.Blue dots indicate examples offluorescence agent.

More than rainbow !Ask any colorthat you want.

RR

GG

BB

VV

UVUV

Feb. 8, 2012 13Ver. 1.0

Pen-type optical heads have been newly developed. It is the best choicefor inspection in production lines or for making various types of hand-heldequipments.


Pen-type optical heads

Probe LED Wavelength-division optics with PD～120

Feb. 8, 2012 14Ver. 1.0

Pen-type optical heads ( cont. )

Optimum design of micro-optics that consists of SELFOC microlenses, wave-length filters, and optical fibers, minimizes the optical noise. It results in theability to work in high ambient light, even in bright sunshine.


0.1

1

10

100

1000

1 10 100 1000

蛍光

強度

[mV

]

FITC濃度[nM]

FITC校正線(純水信号除去)

屋内消灯

直射日光下

FITC density (nM)

Fluo

resc

ence

sig

nal (

mV)

In the dark Under the sun

In the dark Bright room Under the sun

0 20 40 60 (sec)0

10

20

30

40

50

Fluo

resc

ence

sig

nal (

a.u.

)

Take it !Go outside.

Go to the frontline.

Feb. 8, 2012 15Ver. 1.0

Fluorescence detectors: FLE1100NSG’s Fluorescence detector FLE1100 is combining Fluorescence micro-opticswith a controller unit and software, which enables one to obtain fluorescencesignal easily just after buying it.

Wavelength line-up;Excitation Fluorescence

- UV 365nm 470nm- Violet 405nm 470nm- Blue 470nm 530nm- Green 530nm 630nm- Red 630nm 670nm

Please ask what you want

Interface RS232C (to PC), analog voltage (for your requirements)Software attached (easily installed to your PC)Size 130 x 160 x 40mmPower supply AC 100 – 240VDetection area 0.2mm in dia. approximately (customization available)Optical probe 4mm in dia. (Typically)

Feb. 8, 2012 16Ver. 1.0

An example of measurement

Fluorescence signal of the each well of a micro-well plate is measuredin this case.

Fluorescencedetectors FLE1100

Feb. 8, 2012 17Ver. 1.0

Item

Characteristics

FLE1100-02-470/530 FLE1100-02-530/630 FLE1100-02-630/670

Light source(Excitation wavelength)

Blue LED (470 nm)

Green LED (530 nm)

Red LED (630 nm)

Fluorescence wavelength (central value) 530 nm 630 nm 670 nm

Reagent (for example) FITC Resorufin Cy5

Sensitivity

Digital output 1 nmol/ℓ 1 nmol/ℓ 1 nmol/ℓ

Analog output 2 nmol/ℓ 2 nmol/ℓ 2 nmol/ℓ

Dynamic range 27 dB 27 dB 27 dB

Specifications of FLE1100

Sensitivity roughly corresponds to minimum density of the reagents, FITC, Resorufin, and Cy5 withPBS solution, while the fluorescence signal is obtainable with SNR = 10.

NSG’s Fluorescence detector FLE1100 has five types, R, G, B, Violet, and UV,as typical products. Please specify the wavelength what you want.Specifications of R, G, and B-types are shown here as examples;

Feb. 8, 2012 18Ver. 1.0

Features of NSG’s Fluorescence detectors FLE1100 - 1

High sensitivity

Combination of optimized optics andwell-designed controller enables you toobtain high sensitivity in the nM-range.

1

10

100

1000

1 10 100

Density of Cy5 [nM]

Flu

oresc

ence S

ignal

[a.

u.]

0.1

1

10

100

1000

1 10 100 1000

Density of FITC [nM]

Flu

ore

scence S

ignal

[a.

u.]

0.1

1

10

100

1000

1 10 100 1000

Density of Resolufin [nM]

Flu

ore

scence S

ignal

[a.

u.]

FLE1100 - 630/670 (Red LED)

FLE1100 - 530/630 (Green LED)FLE1100 - 470/530 (Blue LED)

FITC, Resorufin, and Cy5 are solved in PBS solution.The results may change corresponding to the mea-surement condition.

Feb. 8, 2012 19Ver. 1.0

Features of NSG’s Fluorescence detectors FLE1100 - 2

Easy to handle, easy to use

Connect the USB cable andcopy attached software toyour PC.Then, just click the icon,you can start the detection.

Feb. 8, 2012 20Ver. 1.0

Fluorescence detectors: FLE500Fluorescence detector FLE500, newly appears as attractive version in cost. The same characteristics as FLE1100 including optics, probe varieties, and color varieties can be obtained with lower cost. Electronics circuit is simpli- fied as analog signal only, and is slightly lower sensitivity.

Wavelength line-up;Excitation Fluorescence

- UV 365nm 470nm- Violet 405nm 470nm- Blue 470nm 530nm- Green 530nm 630nm- Red 630nm 670nm

Please ask what you want

Interface Analog voltageSensitivity 5nM (Approximately)Size 100 x 75 x 30mmPower supply AC 100 – 240VDetection area 0.2mm in dia. approximately (customize available)Optical probe 4mm in dia. (Typically)

Feb. 8, 2012 21Ver. 1.0

Controller- Controllers, circuits, and housings shown here are attached to FLE500.- They can be provided as options with any Fluorescence micro-optics as well.

（※）

DC12VGNDFL signalGNDShield

Fiber

Filter

LED PD

SpecimenProbe

Selfoc microlens

Fiber

Filter

LED PD

SpecimenProbe

Selfoc microlens

Driving circuit with optics including an LED and a PDis mounted inside the controller.

Fluorescencedetectors FLE500

SELFOC microlens

Feb. 8, 2012 22Ver. 1.0

Applications of NSG’s Fluorescence micro-optics / detectors - 1

for bio-chemistry and life-scienceBy putting the optical probes closer to micro-well plates and chemical chips,fluorescence signals can be obtained in various applications such as DNAanalysis, chromatographic measurements, etc.

マイクロタイタープレート

マイクロ化学チップ

プローブ

φ１００ドットスキャン測定時

0

50

100

150

200

1 101 201 301 401 501 601 701 801 901 1001 1101時間［ａ．ｕ．］

蛍光

信号

［ａ．

ｕ．

］

100-1

100-2

100-3

This graph shows a result of fluorescencesignal obtained from specimen dots of0.1mm in diameter.

Micro-well plate

Micro-chemical chip

ProbeProbe

Feb. 8, 2012 23Ver. 1.0

for environmental inspections

- Inspection of pollutant densityin rivers.

- Water flow analysis applyingmulti-point measurements.

Probe

- Inspection of pollutant densityinside hose or tube, caused bybacteria, algae, etc.


Feb. 8, 2012 24Ver. 1.0

for quality inspections in production lines

- Inspections of smearing or leakageof adhesive, grease, lubricant agent,oil, ink, etc.

- Inspections for any other fluorescencematerial such as printed material.

The optical probes enable to inspect small area of 0.2mm in case.They can be placed at the narrow space.

Adhesive

Probe

Lubricantagent onbearings

Probe


Feb. 8, 2012 25Ver. 1.0

The other application candidates - 1

For your next machines …- Real-time PCR - Flowcytometer- micro-plate reader - DNA analyzer - HPLC, High Performance Liquid Chromatography

For the hand-held equipments, multi-point inspections, POCT, etc. …Bio-chemistry and life-science;- infection - glucose - protein - enzyme - bacteria - colon bacillus - influenza - virus - fat - DNA/RNA - allergy - collagen - NADH - odor - fouling- drug - cosmetics - food - cellulose - cholesterol - chlorophyll - cell - amino acid - ELISA- yeast - immunity - skin - plant - vegetable - freshness - blood - urine - sweat - calcium - porphyrin - fungus - fermentation - polyphenol - pollen - microbe - acceptor - oxidation

For example …

Feb. 8, 2012 26Ver. 1.0

For the hand-held equipments, multi-point inspections, etc. …

Environmental inspections;- petroleum - aromatic hydrocarbon - agrichemicals - pesticide - bacteria - industrial effluent - mitochondria - dissolved oxygen - toxin - herbicide - water inspection - coarse-sediment - gas ingredient - soil pollution - algae - phosphoric acid ion

Quality inspections in production lines;- adhesive - lubricant agent - grease - ink - dye - plastic - rubber - paper money - liquid crystal - plating agent - soldering chemicals - filling agent - etching liquid - printing liquid - coating liquid - painting agent - application liquid - deterioration - impurities element - screen-printing ink

For example …

The other application candidates - 2

Feb. 8, 2012 27Ver. 1.0

Let us know what you want.Thank you.

Nippon Sheet Glass Co., Ltd.Information and Telecommunication Device [email protected] Phone: +81-42-775-1546

… not only fluorescence micro-optics, but also the other micro-optics …

This document includes developing prototypes. Please ask us in detail.

mailto:[email protected]

fluorescence micro-optics fluorescence detectors · fluorescence detectors: fle1100 15 - 19....

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