fluorescence micro-optics fluorescence detectors · fluorescence detectors: fle1100 15 - 19....
TRANSCRIPT
Feb. 8, 2012 1Ver. 1.0
*Fluorescence micro-optics*Fluorescence detectors
Nippon Sheet Glass Co., Ltd.Information and Telecommunication Device [email protected] Phone: +81-42-775-1546
Feb. 8, 2012 2Ver. 1.0
Contents
Global info. of Fluorescence micro-optics / detectors 3 - 4
Fluorescence micro-optics 5- Small optical probes for parallel detection 6- Small optical probes for placing in narrow space 7- Wide varieties of optical probes 8 - 10- Customization of optical probes 11- Wide varieties in wavelength 12- Pen-type optical heads 13 - 14
Fluorescence detectors: FLE1100 15 - 19Fluorescence detectors: FLE500 20 - 21
Applications of NSG’s Fluorescence micro-optics / detectors- for bio-chemistry and life-science 22- for environmental inspections 23- for quality inspections in production lines 24
The other application candidates 25 - 26
SELFOC used in this document is registered trademark of Nippon Sheet Glass Co., Ltd.R
2
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*Fluorescence micro-optics*Fluorescence detectorsNSG has developed various Fluorescence micro-optical heads applying muchexperience from SELFOC micro-optics. They are useful for mounting in manykinds of equipment such as real-time PCR machines, etc. and for realizing hand-held equipments. NSG also provides Fluorescence detectors as equipmentthat includes controllers and software. Both Fluorescence micro-optics andFluorescence detectors are commercially available. Please contact with us!
Feb. 8, 2012 4Ver. 1.0
Product line-up
Any component, combination, system can be chosen. Customization is available as well.4
Specimen
Fiber
Specimen
OpticalProbe
ConnectorFilter
LED PDSelfoc microlens
Filter
LED PDS microlens
Fluorescence Fluorescence microopticsmicrooptics
Fluorescence detectorsFluorescence detectors
Electronicscircuit
Software
SELFOC microlens
Feb. 8, 2012 5Ver. 1.0
Fluorescence micro-opticsNSG’s Fluorescence micro-optical heads are based on stacked planar structureof SELFOC lenses and wavelength filters that deriver small, stable, and cost-competitive features.
Fiber
Filter
LED PD
SpecimenProbe
SELFOC microlens
Feb. 8, 2012 6Ver. 1.0
Features of NSG’s Fluorescence micro-optics - 1
Small optical probes for parallel detectionSmall optical probes composed of SELFOC microlenses and fibers canbe placed close to specimen or can be applied to realize array heads.
No more scan …No more 2-D image sensors …
A 16ch probe array of 4.5mmA 16ch probe array of 4.5mm--pitch for 384ch micropitch for 384ch micro--well plates.well plates.
Feb. 8, 2012 7Ver. 1.0
Features of NSG’s Fluorescence micro-optics - 2
Small optical probes for placing in narrow spaceSmall optical probes composed of SELFOC microlenses and fibers canbe placed close to specimen or can be applied to realize array heads.
No matter how narrow …No matter how complicated …
Feb. 8, 2012 8Ver. 1.0
Features of NSG’s Fluorescence micro-optics - 3
Wide varieties of optical probes - 1Wide varieties of optical probes can be lined up by applyingSELFOC microlenses.
Many different eyestake the informationthat you want.Probes of working distances ofaround 1, 2, 4, and 10mm areprepared, corresponding totheir spot sizes of 0.1, 0.2, 0.4,and 1.0mm, respectively.
Workingdistance
Probe 4mm or 3.5mmin diameter
WD profile of the fluorescence signal(Sample thickness=1mm)
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
0 1 2 3 4 5WD (mm)
Fluo
resc
ence
sig
nal
[a.
u.]
4020
4010
4040
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Features of NSG’s Fluorescence micro-optics - 3
Wide varieties of optical probes - 2Thinner probes of 1.25, 0.75, and 0.25mm in dia. have also been developed.
WD profile of the fluorescence signal(Sample thickness=1mm)
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
0.0 0.5 1.0 1.5 2.0 2.5WD (mm)
Fluo
resc
ence
sig
nal [
a.u.
]
φ0.25
φ0.75
φ1.25
φ1.25
φ1.25
φ1.25
Not a needle, but a probe …
Feb. 8, 2012 10Ver. 1.0
Features of NSG’s Fluorescence micro-optics - 3
Line-up of optical probes
We will also provide a custom product to meet your requirements.
No. Probe dia. Spot dia. W.D.
40100 4.0 (mm) 1.0 (mm) 10 (mm)4040 4.0 0.4 44020 4.0 0.2 24010 4.0 0.1 11210 1.25 0.5 11208 1.25 0.4 0.81206 1.25 0.3 0.60702 0.75 0.4 0.20200 0.25 0.2 0
No. Probe dia. Spot dia. W.D.
40100 4.0 (mm) 1.0 (mm) 10 (mm)4040 4.0 0.4 44020 4.0 0.2 24010 4.0 0.1 11210 1.25 0.5 11208 1.25 0.4 0.81206 1.25 0.3 0.60702 0.75 0.4 0.20200 0.25 0.2 0
Feb. 8, 2012 11Ver. 1.0
Features of NSG’s Fluorescence micro-optics - 4
Customization of optical probesIn case you do not find a suitable product in our line-up, we will providea custom product to meet your requirements.
NSG’s vast experienceand knowledge of micro-optics provides the bestsolution.
Excitation beam profile
Excitation beam
Specimen
Z
[1]Y
X
Selfoc probe
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
0 1 2 3 4 5 6 7 8
WD (mm)
Fluo
resc
ence
sign
al [
a.u.
]
Sample-t : 1mm(Simulation)Sample-t : 2mm(Simulation)Sample-t : 3mm(Simulation)Sample-t : 1mm(Measurement data)Sample-t : 2mm(Measurement data)Sample-t : 3mm(Measurement data)
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
WD (mm)
Fluo
resc
ence
sign
al[
a.u.
]
[1]
[2]
[3]
Three-typesof probesdesigned atWD = 3 – 4mm.Lines: estimatedDots: measured
Three-typesof probesdesigned atWD = 18 – 24mm.
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400 500 600 700 300
400
50
0
6
00
700
Exci
tatio
n (n
m)
Fluorescence (nm)
Features of NSG’s Fluorescence micro-optics - 5
Wide varieties in wavelength Wide varieties in wavelength of both excitation and fluorescence are available by integrating SELFOCmicrolenses with small size filters.
Five standard lineups ( paintedrectangles ) and semi-customs( many other rectangles ) coverany position in the wavelengthcoordinates.Blue dots indicate examples offluorescence agent.
More than rainbow !Ask any colorthat you want.
RR
GG
BB
VV
UVUV
Feb. 8, 2012 13Ver. 1.0
Pen-type optical heads have been newly developed. It is the best choicefor inspection in production lines or for making various types of hand-heldequipments.
Features of NSG’s Fluorescence micro-optics - 6
Pen-type optical heads
Probe LED Wavelength-division optics with PD~120
Feb. 8, 2012 14Ver. 1.0
Pen-type optical heads ( cont. )
Optimum design of micro-optics that consists of SELFOC microlenses, wave-length filters, and optical fibers, minimizes the optical noise. It results in theability to work in high ambient light, even in bright sunshine.
Features of NSG’s Fluorescence micro-optics - 6
0.1
1
10
100
1000
1 10 100 1000
蛍光
強度
[mV
]
FITC濃度[nM]
FITC校正線(純水信号除去)
屋内消灯
直射日光下
FITC density (nM)
Fluo
resc
ence
sig
nal (
mV)
In the dark Under the sun
In the dark Bright room Under the sun
0 20 40 60 (sec)0
10
20
30
40
50
Fluo
resc
ence
sig
nal (
a.u.
)
Take it !Go outside.
Go to the frontline.
Feb. 8, 2012 15Ver. 1.0
Fluorescence detectors: FLE1100NSG’s Fluorescence detector FLE1100 is combining Fluorescence micro-opticswith a controller unit and software, which enables one to obtain fluorescencesignal easily just after buying it.
Wavelength line-up;Excitation Fluorescence
- UV 365nm 470nm- Violet 405nm 470nm- Blue 470nm 530nm- Green 530nm 630nm- Red 630nm 670nm
Please ask what you want
Interface RS232C (to PC), analog voltage (for your requirements)Software attached (easily installed to your PC)Size 130 x 160 x 40mmPower supply AC 100 – 240VDetection area 0.2mm in dia. approximately (customization available)Optical probe 4mm in dia. (Typically)
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An example of measurement
Fluorescence signal of the each well of a micro-well plate is measuredin this case.
Fluorescencedetectors FLE1100
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Item
Characteristics
FLE1100-02-470/530 FLE1100-02-530/630 FLE1100-02-630/670
Light source(Excitation wavelength)
Blue LED (470 nm)
Green LED (530 nm)
Red LED (630 nm)
Fluorescence wavelength (central value) 530 nm 630 nm 670 nm
Reagent (for example) FITC Resorufin Cy5
Sensitivity
Digital output 1 nmol/ℓ 1 nmol/ℓ 1 nmol/ℓ
Analog output 2 nmol/ℓ 2 nmol/ℓ 2 nmol/ℓ
Dynamic range 27 dB 27 dB 27 dB
Specifications of FLE1100
Sensitivity roughly corresponds to minimum density of the reagents, FITC, Resorufin, and Cy5 withPBS solution, while the fluorescence signal is obtainable with SNR = 10.
NSG’s Fluorescence detector FLE1100 has five types, R, G, B, Violet, and UV,as typical products. Please specify the wavelength what you want.Specifications of R, G, and B-types are shown here as examples;
Feb. 8, 2012 18Ver. 1.0
Features of NSG’s Fluorescence detectors FLE1100 - 1
High sensitivity
Combination of optimized optics andwell-designed controller enables you toobtain high sensitivity in the nM-range.
1
10
100
1000
1 10 100
Density of Cy5 [nM]
Flu
oresc
ence S
ignal
[a.
u.]
0.1
1
10
100
1000
1 10 100 1000
Density of FITC [nM]
Flu
ore
scence S
ignal
[a.
u.]
0.1
1
10
100
1000
1 10 100 1000
Density of Resolufin [nM]
Flu
ore
scence S
ignal
[a.
u.]
FLE1100 - 630/670 (Red LED)
FLE1100 - 530/630 (Green LED)FLE1100 - 470/530 (Blue LED)
FITC, Resorufin, and Cy5 are solved in PBS solution.The results may change corresponding to the mea-surement condition.
Feb. 8, 2012 19Ver. 1.0
Features of NSG’s Fluorescence detectors FLE1100 - 2
Easy to handle, easy to use
Connect the USB cable andcopy attached software toyour PC.Then, just click the icon,you can start the detection.
Feb. 8, 2012 20Ver. 1.0
Fluorescence detectors: FLE500Fluorescence detector FLE500, newly appears as attractive version in cost. The same characteristics as FLE1100 including optics, probe varieties, and color varieties can be obtained with lower cost. Electronics circuit is simpli- fied as analog signal only, and is slightly lower sensitivity.
Wavelength line-up;Excitation Fluorescence
- UV 365nm 470nm- Violet 405nm 470nm- Blue 470nm 530nm- Green 530nm 630nm- Red 630nm 670nm
Please ask what you want
Interface Analog voltageSensitivity 5nM (Approximately)Size 100 x 75 x 30mmPower supply AC 100 – 240VDetection area 0.2mm in dia. approximately (customize available)Optical probe 4mm in dia. (Typically)
Feb. 8, 2012 21Ver. 1.0
Controller- Controllers, circuits, and housings shown here are attached to FLE500.- They can be provided as options with any Fluorescence micro-optics as well.
(※)
DC12VGNDFL signalGNDShield
Fiber
Filter
LED PD
SpecimenProbe
Selfoc microlens
Fiber
Filter
LED PD
SpecimenProbe
Selfoc microlens
Driving circuit with optics including an LED and a PDis mounted inside the controller.
Fluorescencedetectors FLE500
SELFOC microlens
Feb. 8, 2012 22Ver. 1.0
Applications of NSG’s Fluorescence micro-optics / detectors - 1
for bio-chemistry and life-scienceBy putting the optical probes closer to micro-well plates and chemical chips,fluorescence signals can be obtained in various applications such as DNAanalysis, chromatographic measurements, etc.
マイクロタイタープレート
マイクロ化学チップ
プローブ
φ100ドットスキャン測定時
0
50
100
150
200
1 101 201 301 401 501 601 701 801 901 1001 1101時間[a.u.]
蛍光
信号
[a.
u.
]
100-1
100-2
100-3
This graph shows a result of fluorescencesignal obtained from specimen dots of0.1mm in diameter.
Micro-well plate
Micro-chemical chip
ProbeProbe
Feb. 8, 2012 23Ver. 1.0
for environmental inspections
- Inspection of pollutant densityin rivers.
- Water flow analysis applyingmulti-point measurements.
Probe
- Inspection of pollutant densityinside hose or tube, caused bybacteria, algae, etc.
Applications of NSG’s Fluorescence micro-optics / detectors - 2
Feb. 8, 2012 24Ver. 1.0
for quality inspections in production lines
- Inspections of smearing or leakageof adhesive, grease, lubricant agent,oil, ink, etc.
- Inspections for any other fluorescencematerial such as printed material.
The optical probes enable to inspect small area of 0.2mm in case.They can be placed at the narrow space.
Adhesive
Probe
Lubricantagent onbearings
Probe
Applications of NSG’s Fluorescence micro-optics / detectors - 3
Feb. 8, 2012 25Ver. 1.0
The other application candidates - 1
For your next machines …- Real-time PCR - Flowcytometer- micro-plate reader - DNA analyzer - HPLC, High Performance Liquid Chromatography
For the hand-held equipments, multi-point inspections, POCT, etc. …Bio-chemistry and life-science;- infection - glucose - protein - enzyme - bacteria - colon bacillus - influenza - virus - fat - DNA/RNA - allergy - collagen - NADH - odor - fouling- drug - cosmetics - food - cellulose - cholesterol - chlorophyll - cell - amino acid - ELISA- yeast - immunity - skin - plant - vegetable - freshness - blood - urine - sweat - calcium - porphyrin - fungus - fermentation - polyphenol - pollen - microbe - acceptor - oxidation
For example …
Feb. 8, 2012 26Ver. 1.0
For the hand-held equipments, multi-point inspections, etc. …
Environmental inspections;- petroleum - aromatic hydrocarbon - agrichemicals - pesticide - bacteria - industrial effluent - mitochondria - dissolved oxygen - toxin - herbicide - water inspection - coarse-sediment - gas ingredient - soil pollution - algae - phosphoric acid ion
Quality inspections in production lines;- adhesive - lubricant agent - grease - ink - dye - plastic - rubber - paper money - liquid crystal - plating agent - soldering chemicals - filling agent - etching liquid - printing liquid - coating liquid - painting agent - application liquid - deterioration - impurities element - screen-printing ink
For example …
The other application candidates - 2
Feb. 8, 2012 27Ver. 1.0
Let us know what you want.Thank you.
Nippon Sheet Glass Co., Ltd.Information and Telecommunication Device [email protected] Phone: +81-42-775-1546
… not only fluorescence micro-optics, but also the other micro-optics …
This document includes developing prototypes. Please ask us in detail.