FLORIDA CORE NETWORK EVENT 3 PROGRAM

CHEMICAL SCIENCES LABORATORY

FLORIDA STATE UNIVERSITY, TALLAHASSEE, FL 32306

8/11/2016 Third Annual Meeting

Organizers: FSU-Wide Core Scientific Facilities Contact: Thayumana Somasundaram

www.biophysics.fsu.edu/fcne3

The program of the Florida Core Network Event 3 held at Florida State

University’s Chemical Sciences Laboratory on Thursday, August 11, 2016

between 9:30 AM and 5 PM.

Florida Core Network Event 3 Program

Florida State University, Tallahassee, FL 32306 August 11, 2016 Third Annual Event

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EVENT SPONSORS AND SUPPORT

EXCLUSIVE SPONSOR

ADDITIONAL SPONSORS

FSU-WIDE CORE FACILITIES PERSONNEL

ADDITIONAL SUPPORT

Florida Core Network Event 3 Program

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CONTENTS

THIRD ANNUAL MEETING ....................................................................................................................... 3

Event schedule, information, and location ................................................................................................................. 3

FSU CORE FACILITIES AND ITS PERSONNEL ............................................................................................ 5

GROUP PHOTO ....................................................................................................................................... 5

TRANSPORTATION FOR OFF CAMPUS TOUR SERVICE ........................................................................... 5

ON CAMPUS TOUR ................................................................................................................................. 6

OFF CAMPUS TOUR ................................................................................................................................ 6

POSTER RECEPTION ................................................................................................................................ 7

MAPS AND PARKING .............................................................................................................................. 7

MAG LAB AND FSU CAMPUS MAPS........................................................................................................ 8

FSU CORE FACILITY DETAILS ................................................................................................................... 9

ACKNOWLEDGEMENTS ......................................................................................................................... 12

MEETING ATTENDEES ............................................................................................................................ 14

NOTES ................................................................................................................................................... 17

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FLORIDA CORE NETWORK EVENT 3 PROGRAM

THIRD ANNUAL MEETING

EVENT SCHEDULE, INFORMATION, AND LOCATION

All events will be held in CSL 1003 unless specified otherwise Presentations in black color; Concurrent sessions in blue color; Common items in garnet color

Item Start Time

End Time

Duration Information and location

Thursday, August 11, 2016

Coffee 8:30 AM 9:30 AM 1:00 CSL Lobby

Registration 8:30 AM 9:30 AM 1:00 CSL Lobby

Opening Remarks 9:30 AM 9:40 AM 0:10 Gary Ostrander, VP Research

Welcome and announcements 9:40 AM 9:50 AM 0:10 Thayumanasamy Somasundaram

Speed Introductions (Host: )

Quick Introductions 9:50 AM 10:30 AM 0:40 Introduction of Core Facility Personnel

Lightning Round -1 (Host: Thayumanasamy Somasundaram )

Becky Augustyniak 10:30 AM 10:40 AM 0:10 Florida ExpertNet: Connecting with Expertise and Resources Across Florida’s Universities

Kari Basso 10:40 AM 10:45 AM 0:05 University of Florida – Mass Spectrometry

Services

Duncan Sousa 10:45 AM 10:50 AM 0:05 The Biological Science Imaging Resource and the South Eastern Consortium for Microscopy

of Macro Molecular Machines

10:50 AM 10:55 AM 0:05

Thayumanasamy Somasundaram

10:55 AM 11:00 AM 0:05 FSU Scientific Asset Management Database

and Webportal

Coffee Break 11:00 AM 11:15 AM 0:15 CSL Lobby

Topics in Industry Trends

Industry Trends 11:15 AM 11:45 AM 0:30 Catalyst Group VWR (Host: Lynn Moore)

CSL 1003

Round Table Discussions (Joan Hare and Huan He)

Core Facility Round Tables (0:40 min discussions;

0:05 min summary; 0:02 min/group overall update)

11:45 AM 12:45 PM 1:00

CSL 1005

Managing Instrument Repair Costs/Are Service Contracts Worth the Cost?

Roger Mercer

Billing and Collecting for Core Services: A Continuing Drain on Our Time?

Cheryl Pye

Coordination Between Cores; How Important Is It?

Claudius Mundoma

Developing a Good Client/Core Director Relationship/How can you do it?

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Huan He

Do you want to be a Green Lab? Practicing Sustainability in the Lab

Renee Murray

Difficulties and Opportunities in Introducing New Technologies into The Core Thayumanasamy Somasundaram

How Can We Build New Client Base? Joan Hare

Finding Funding for Core Facilities Duncan Sousa

Group Photo 12:45 PM 12:50PM 0:05 CSL Entrance or CSL Lobby (if raining)

Lunch (Steven Miller and Margaret Seavy)

Lunch and Vendor Show 12:45 PM 2:00 PM 1:15 Lunch and Vendor Showcase (CSL Lobby)

Lightning Round-2 (Host: Claudius Mundoma)

Panel Discussion 2:00 PM 2:45 PM 0:45

Panel Discussion (CSL 1003)

“Maintaining a good relationship between the administration and core facility”

Susan Constable, Management Claudius Mundoma, Core Director

Brian Washburn 2:45 PM 2:50 PM 0:05 Biological Science Cores

Claudius Mundoma 2:50 PM 2:55 PM 0:05 Physical Biochemistry Facility

Joan Hare 2:55 PM 3:00 PM 0:05 Protein Expression Facility

Roger Mercer 3:00 PM 3:05 PM 0:05 CoM Translational Lab

3:05 PM 3:10 PM 0:05

Coffee Break 3:10 PM 3:25 PM 0:15 CSL Lobby

Off Campus Tour 1 (Host: )

Off-Campus Tour 3:30 PM 5:00 PM 1:30 Mag Lab, HPMI, and LSI

On Campus Tour 1 (Hosts: Roger Mercer, Cheryl Pye, Duncan Sousa)

On-Campus Tour 1 3:30 PM 5:00 PM 1:30 CoM, Biology, and BSIR

On Campus Tour 2 (Hosts: Bert van de Burgt, Claudius Mundoma, Eric Lochner)

On-Campus Tour 2 3:30 PM 5:00 PM 1:30 Chemistry, IMB, and Physics

Poster (Brian Washburn)

Poster 5:00 PM 8:00 PM 3:00 CSL Lobby and CSL 1000

Dinner (Margaret Seavy and Steven Miller) Reception (Somasundaram)

Reception 5:00 PM 8:00 PM 3:00 CSL Lobby and CSL 1000

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FSU CORE FACILITIES AND ITS PERSONNEL

First Name Last Name Facility E-mail Phone

Beth Alexander Cell Culture Facility beth.alexander@med.fsu.edu 850-645-2931

John Beidler College of Medicine john.beidler@med.fsu.edu 850-644-9947

Banghao Chen NMR Lab chen@chem.fsu.edu 850-644-3334

Ruth Didier Flow Cytometry &

Confocal ruth.didier@med.fsu.edu 850-645-1465

Dano Fiore Biology fiore@bio.fsu.edu 850-644-9697

Steven E Freitag NMR Lab sfreitag@chem.fsu.edu 850-644-5586

Umesh Goli Mass Spec Lab ugoli@chem.fsu.edu 850-644-1697

Joan Hare Protein Expression jhare@fsu.edu 850-644-1421

Huan He Translational Lab hhe@fsu.edu 850-645-9842

Xixi Jia Psychology jia@psy.fsu.edu 850-644-6807

Eric Lochner Physics lochner@physics.fsu.edu 850-644-3456

Alecia Lapointe fMRI alecia.lapointe@med.fsu.edu 508-353-0148

Roger Mercer Translational Lab roger.mercer@med.fsu.edu 850-645-9598

Steve Miller DNA Sequencing smiller@bio.fsu.edu 850-644-8956

Claudius Mundoma Physical Biochem cmundoma@fsu.edu 850-644-5147

Kristina Poduch Hybridoma poduch@bio.fsu.edu 850-644-0575

Cheryl Pye Molecular Cloning fap13c@my.fsu.edu 850-644-0575

Margaret Seavy Bioanalytical seavy@bio.fsu.edu 850-644-5444

Rakesh Singh Translational Lab rakesh.singh@med.fsu.edu 850-645-9813

Thayumanasamy Somasundaram X-Ray

Crystallography tsomasundaram@fsu.edu 850-644-6448

Duncan Sousa BSIR dsousa@fsu.edu 850-644-6519

John Spear BSIR jms09z@fsu.edu 850-644-6519

Bert van de Burgt Mat Characterization vandeburgt@chem.fsu.edu 850-644-3468

Daniel Vera Center for Genomics vera@genomics.fsu.edu 850-644-9711

Cynthia Vied Translational Lab cynthia.vied@med.fsu.edu 850-645-8491

Brian Washburn Hybridoma washburn@bio.fsu.edu 850-644-0575

Bonnie Wright Psychology wright@psy.fsu.edu 850-645-8192

Yanming Yang Translational Lab yanming.yang@med.fsu.edu 850-645-0019

Michael Zawrotny Computer Resources mzawrotny@fsu.edu 850-644-0069

GROUP PHOTO A group photo of the meeting attendees will be taken during the Lunch Break around 12:45 PM. Group Photo in front of CSL or

CSL Lobby (if raining).

TRANSPORTATION FOR OFF CAMPUS TOUR SERVICE There will be shuttle service between the Chemical Sciences Laboratory and the National Magnet Lab. The shuttle will leave

around 3:30 PM from CSL Parking Lot towards the National Magnet Lab. The return trip will start at the Magnet lab around

4:45 PM towards the CSL.

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ON CAMPUS TOUR

OFF CAMPUS TOUR

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POSTER RECEPTION The poster session with a reception will be starting at 5:00 PM in CSL 1004 and 1000. People are encouraged to display their

posters at their earliest convenience at CSL 1000.

MAPS AND PARKING

Map of Chemical Sciences Laboratory and surrounding buildings (GPS: 30.443884, -84.300966)

Event Parking Map (Park only in “W” lots and not “R” lots)

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Map of first floor of CSL (Event Location)

MAG LAB AND FSU CAMPUS MAPS

Mag Lab floor plan. http://magnet.fsu.edu/about/visit.html

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FSU Campus Map

FSU CORE FACILITY DETAILS

Biology Analytical Laboratory | http://www.bio.fsu.edu/analytical.php

Margaret Seavy, Biological Science, Florida State University, FL 32306-4295, USA

1-850-644-5444 | seavy@bio.fsu.edu

The Analytical Lab incorporates state-of-the-art laboratory equipment for performing biological analyses, developing methods to

aid in research, and training faculty and graduate students in equipment operation and research techniques. The facility offers

HPLC and Protein sequencing services as well as centrifuges (high-speed and ultra), a Bio-Rad Chemi-Doc, an Agilent

Bioanalyzer, a Bio-Rad Experion, a GE Typhoon Imager, a Nanodrop, a multimodal Spectra-max M5 plate reader, and a Bio-

Mek 4000 liquid handling station.

Biology DNA Sequencing Facility | http://www.bio.fsu.edu/dna_sequencing.php

Steven H. Miller, Biological Science, Florida State University, FL 32306-4295, USA

1-850-644-3601 | smiller@bio.fsu.edu

The DNA Sequencing Facility offers sequencing and fragment analysis services using an Applied Biosystems 3730 Genetic Analyzer with Capillary Electrophoresis. DNA sequencing chemistries are performed using Big-Dye terminator v3.1 on double

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or single stranded DNA and PCR products. We also offer Next Generation Sequencing utilizing an Illumina MiSeq. The facility is home to an ABI 7500 Fast Real-Time PCR system and several common use Veriti PCR machines.

Biological Science Imaging Resource | http://bsir.bio.fsu.edu/

Duncan Sousa and John Spear, Biological Science, Florida State University, FL 32306-4295, USA

1-850-644-6519 | dsousa@fsu.edu

The Biological Science Imaging Resource (BSIR) is dedicated to making state of the art electron microscopy (EM) and confocal

microscopy available to everyone without multimillion dollar investments in instruments and facilities. The center piece of the

BSIR is the FEI’s Titan Krios equipped with the DE64 detector through which we offer affordable, state of the art, remote robotic

TEM imaging for local users as well as the South Eastern Consortium for Microscopy of Macro Molecular Machines (SECM4).

SECM4 is an NIH funded facility that supports 18 cryoEM investigators from institutions outside of Florida State University. With

the Appion automated data collection system, users can view data collected in real time via a web browser. We also offer

scanning electron microscopy and e- beam lithography through the FEI Nova NanoSEM, laser scanning dual photon confocal

imaging using Zeiss’s LSM 510 confocal microscope, and bright field and fluorescent light microscopy.

Biology Hybridoma Facility | http://www.bio.fsu.edu/hybridoma.php

Brian Washburn and Kristina Poduch, Biological Science, Florida State University, FL 32306-4295, USA

1-850-644-0575 | mcf@bio.fsu.edu

The Hybridoma Facility provides the expertise and assistance necessary for the production of monoclonal antibodies. This includes the immunization of mice, collection and testing of immune sera, hybridoma fusions, screening of hybridomas by ELISA, subcloning of selected cell lines, scale up of cells for supernatants, mycoplasma testing, isotyping, and long term storage of hybridomas and revival as needed.

Biology Molecular Cloning Facility | http://www.bio.fsu.edu/mol_cloning.php

Brian Washburn, Cheryl Pye, Kristina Poduch, Biological Science, Florida State University, FL 32306-4295, USA

1-850-644-0575 | mcf@bio.fsu.edu

The Molecular Cloning Facility provides expertise and assistance in designing and implementing molecular biology techniques. This includes training to support individuals with their research projects, full-service production of plasmid clones, mutants, and fusions constructs, libraries, qPCR analysis of DNA and RNA samples from a variety of organisms.

Chem & Biochem Mass Spectrometry Facility | http://www.chem.fsu.edu/facilities.php?facility_id=12&info=details Umesh Goli, Chemistry and Biochemistry, Florida State University, FL 32306-4390, USA

1-850-644-1697 | ugoli@chem.fsu.edu

The Mass Spectrometry Laboratory has Accu TOF and MALDI TOF Time of Flight Mass Spectrometers, LC-Tandem Mass spectrometer, GC-MS, Capillary Gas Chromatograph, and Liquid Chromatographs and with Light Scattering Detector. Chem & Biochem Material Characterization Lab | www.chem.fsu.edu/facilities.php?facility_id=13&info=details Bert van de Burgt, Chemistry and Biochemistry, Florida State University, FL 32306-4390, USA

1-850-644-3468 | vandeburgt@chem.fsu.edu

The Materials Characterization Laboratory is a multiuser research facility allowing access to a variety of sophisticated instruments to allow the characterization of materials. The facility is well equipped to carry out magnetic property research using a SQUID/Evercool MPMS magnetometer and two liquid helium cooled magnetometers (MPMS & PPMS), x-ray diffraction of powders and single crystals, a variety of optical spectrometers (UV/VIS/NIR, FT-IR, fluorescence) and thermal analysis (SDT & TGA), atomic force and optical microscopy, Raman vibrational spectroscopy using continuous wave (cw) lasers and transient absorption and emission spectroscopy using pulsed lasers.

Chem & Biochem NMR Lab | http://www.chem.fsu.edu/facilities.php?facility_id=15&info=details Banghao Chen and Steven Freitag, Chemistry and Biochemistry, Florida State University, FL 32306-4390, USA

1-850-644-3334 | chen@chem.fsu.edu

NMR is one of the most powerful non-destructive spectroscopic analytical techniques, which can be used to determine the structures of chemical compounds. Modern NMR spectroscopy has been widely applied in many disciplines of scientific

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research, medicine, and various industries. Such as: chemistry, biology, material science, medicine, petroleum industry, and environmental studies, etc.

CoM Cell Culture Facility | http://med.fsu.edu/index.cfm?page=cellCultureFacility.home Beth Alexander, College of Medicine, Florida State University, FL 32306, USA

1-850-645-2931 | beth.alexander@med.fsu.edu

The Facility is designed to accommodate the culture of mammalian cell lines and other animal cell lines, such as insect cells. Work involving the culture of bacterial cells, spore-forming yeast, Neurospora or fungi or single-celled microorganisms may not be performed due to the potential of these cultures contaminating other cell lines. All work involving human cell lines must first be approved by the University Biosafety Committee. All work involving recombinant DNA must first be approved by the University Recombinant DNA Committee. All work is reviewed for appropriateness of the work for the Facility.

CoM Flow Cytometry Laboratory | http://med.fsu.edu/index.cfm?page=flowCytometryLab.home

Ruth Didier, College of Medicine, Florida State University, FL 32306, USA

1-850-645-1465 | ruth.didier@med.fsu.edu

Flow Cytometry consists of two instruments. BD FACSCanto SOS is a 4 laser 9 PMT analyzer. BD FACSAria SORP is a 4 laser

13 PMT sorter. Both systems can analyze cell cycle, percentages of fluorescent proteins or antibodies etc. Anything that can

be analyzed is capable of bulk sorting into tubes, single cell sorting into 96 well plates or sorting onto a slide using the FACSAria

SORP.

CoM Confocal Microscopy Laboratory | http://med.fsu.edu/index.cfm?page=confocalMicroscopyLab.home

Ruth Didier, College of Medicine, Florida State University, FL 32306, USA 1-850-645-2920 | ruth.didier@med.fsu.edu

There are 3 instruments in the Confocal Microscopy Facility. Andor spinning disk confocal, Zeiss LSM 880, and GEHealthcare

OMX Blaze super resolution. All systems have inverted microscopes Each microscope has different capabilities. See website

for more information.

CoM Translational Science Laboratory | http://med.fsu.edu/index.cfm?page=translationalLab.home

Roger Mercer, Yanming Yang, Rakesh Singh, and Huan He, College of Medicine, FSU, FL 32306, USA

1-850-645-9598 | roger.mercer@med.fsu.edu

Medical scientists are focused now on translating two decades of biomedical research into cures for human disease. The

Translational Science Laboratory provides the physical space, the applied technologies and the staff to serve medical scientists

at Florida State and elsewhere in their search for targets for treatment of disease.

IMB Protein Expression Facility | http://biophysics.fsu.edu/facilities/protein-expression-facility/

Joan Hare, Institute of Molecular Biophysics, Florida State University, FL 32306-4380, USA

1-850-644-1421 | jhare@fsu.edu

The Protein Expression Facility (PEF) is a multi-user facility which serves primarily faculty and students from the laboratories in

the Kasha Laboratory Building. The primary purpose of the PEF is to facilitate the large scale expression of recombinant proteins

in eucaryotic cells. We have methods and equipment for that purpose, including the WAVE bioreactor and the BelloCell

bioreactor. Other projects include expression of native proteins in response to chemical or biochemical inducers or metabolic

pressures, uptake of nanoparticles and dyes, production of siRNA knockout gene clones and analysis of effects, and cytological

studies of the cellular location of novel proteins.

IMB Physical Biochemistry Facility | http://biophysics.fsu.edu/facilities/physical-biochemistry-facility/

Claudius Mundoma, Institute of Molecular Biophysics, Florida State University, FL 32306-4380, USA

1-850-644-5147 | cmundoma@fsu.edu

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The Physical Biochemistry Facility (PBF) provides state-of-the-art resources for biophysical studies of macromolecules. Special emphasis is placed on providing resources, training, and guidance to graduate and undergraduate students. The PBF offers access to a broad range of instrumentation that is usually not available in an individual laboratory. Consequently, students have the opportunity to familiarize themselves with different biophysical techniques and evaluate their suitability to a particular study. The PBF also provides a forum for communication and exchange of ideas among students in their research efforts. IMB X-Ray Crystallography Facility | http://biophysics.fsu.edu/facilities/x-ray-facility/ Thayumanasamy Somasundaram, Institute of Molecular Biophysics, Florida State University, FL 32306-4380, USA

1-850-644-6448 | tsomasundaram@fsu.edu

As one of the FSU-wide Core Facilities, the multi-user macromolecular x-ray crystallography facility (XRF) at the Kasha

Laboratory Building (KLB) in the Institute of Molecular Biophysics (IMB) provides training, assistance, and expertise for

crystallizing and determining the three-dimensional structures of biological macromolecules and their complexes. It is available

to all the members of Florida State University and other members of Florida State University System. XRF is located on the East

side of the fourth floor of KLB. More facility information is available at X-Ray Facility page. All the users of the Facility are charged

a Usage Fee and fee structure depends on the nature and length of usage. To encourage new users and expand the user base

exploratory use will remain free of charge.

ACKNOWLEDGEMENTS The organizers of the event would like to acknowledge the following people for bringing the event to Florida State University: All

the members of FSU-wide Core Facilities; Dr. Piotr Fajer, Director, IMB; Dr. Timothy Logan, Chairman, Chemistry and

Biochemistry; Office of Research; Gustavo Galdo and Lynn Moore, VWR; Susan Constable, Moffitt Cancer Center; Connie

Nicklin, ICBR, UF; Wade Baggett, Rick Wang, and Regina Ware, IMB; Makisha Howell, Mike Tharp, Keith Collins, Gary Poplin,

and Nodin Waddington, Chemistry and Biochemistry; Paulina Lewis and Cindi Majszak, Seminole Dining; Patricia Allaire

Catalina Café; Devin, Real Paella; Brian Fairhurst, Renee Murray, Kevin Thompson, Colleen Thomas, Lisa McGrady and Carla

Ross, FSU,

POSTERS

Rebecca Augustyniak, Director, Florida ExpertNet, raugustyniak@fsu.edu; (850) Florida ExpertNet is a free statewide portal of research expertise across Florida’s universities. ExpertNet is operated in

partnership with the SUS Board of Governors and includes the following: Faculty experts and their funded research projects,

Specialized centers and institutes, Licensing and commercialization opportunities, Speakers on a variety of topics, Postsecondary

instructional programs in the user’s area, and Innovation Exchange matchmaking portal for businesses.

Alberto Riva, Associate Scientist, ariva@ufl.edu, ICBR Bioinformatics Core Reliability, reproducibility, and reusability are essential requirements for analysis pipelines, especially in the context of a

bioinformatics core facility tasked with analyzing a large number of diverse datasets. We are developing a software platform

for the rapid development of analysis pipelines in a high-performance computing setting. The platform is based on Actor, an

object-oriented Python library that allows developers to quickly create complex analysis pipelines, by combining standard

Python code with predefined objects and methods implementing specific analysis tasks (e.g., different sequence alignment

programs). These building blocks only need to be developed and debugged once, leading to high reliability and reusability.

Actor is designed to work in a cluster environment, and provides methods to submit jobs to a queue and wait for their termination

under either PBS or Slurm. It therefore facilitates the creation of complex pipelines consisting of multiple processes running

sequentially or in parallel. Finally, Actor pipelines automatically generate a publication-quality report describing all steps of

the analysis, including figures and links to all relevant input and output files.

Leigh West, Scientific Researcher, westl@usf.edu. (813) 974-6270 The Center for Drug Discovery and Innovation (CDDI) is a Florida Center of Excellence that serves and collaborates with researchers from USF and the biotechnology community. From concept to clinical trials, significant challenges arise for discovery of new drugs and treatments for human disease. Our mission is to assist research groups during the critical bottleneck of early phase drug discovery. While achieving this mission, students and collaborators are trained in different aspects of experimental design, sample preparation and data collection/interpretation. Thus, the CDDI operates on the principle that success is best achieved through active partnership in a setting that trains the individual student/researcher to work independently and to

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develop new skills and knowledge. To overcome the critical bottleneck a wide choice of analytical methods may be applied to the general analysis of all components from cells or tissues. Equipment and expertise is found in our four core facilities: Mass Spectrometry, Protein Production and Cell Biology, NMR, Chemodiversity.

Thayumanasamy Somasundaram, X-Ray Facility Director, tsomasundaram@fsu.edu; (850) 644-6448 The x-ray crystallography facility provides the instrument and expertise for screening macromolecular crystallization conditions

and optimization. We can collect x-ray diffraction data from macromolecular single crystals both using home and synchrotron

sources. We can solve the 3-D structure of your interest that will provide mechanistic and binding details of your macromolecule.

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MEETING ATTENDEES

Attendees

Amer Abouhamze Assistant Director CTSI Biorepository Amer@ufl.edu (352) 273-9498 University of Florida Beth Alexander Research Coordinator beth.alexander@med.fsu.edu Biomedical Sciences Cell Culture Facility Florida State University Rebecca Augustyniak Director Florida ExpertNet raugustyniak@fsu.edu (850) 644-5602 CIMES Florida State University Ramesh Badisa Research Associate badisa66@gmail.com Biotechnology Core Florida A&M University Ken Baldauf Director, Program in Interdisciplinary Computing kbaldauf@fsu.edu (850) 645-8649 Florida State University Brad Barbazuk Associate Professor & Faculty Scientific Director ICBR Bioinformatics Core group bbarbazuk@ufl.edu (352) 273-8624 University of Florida Kari Basso Director Mass Spectrometry Services kari.basso@chem.ufl.edu University of Florida

John Beidler Director, Facilities and Operations College of Medicine john.beidler@med.fsu.edu (850) 644-9947 Florida State University Neal Benson Scientific Director nbenson@ufl.edu (352) 273-8186 ICBR-Cytometry University of Florida Matthew Booth Assistant Scientist/Technical Director UF Analytical Toxicology Core mmbooth@ufl.edu (352) 294-4585 University of Florida Maria Boulina mboulina@med.miami.edu (305) 243 8436 AICF University of Miami Jhunu Chatterjee Associate Director jchatterjee@fsu.edu (850) 644-1637 Ctr Undergraduate Research and Academic Engagement Florida State University Banghao Chen Director of NMR Facility chen@chem.fsu.edu FSU On-campus NMR Facility FSU Paul Chipman Scientific Director EM Core pchipman@ufl.edu ICBR University of Florida

Susan Constable Manager, Basic Science Shared Resources susan.constable@moffitt.org (813) 745-8892 H Lee Moffitt Cancer Center & Research Institute Christopher Cubitt Core staff scientist christopher.cubitt@moffitt.org (813) 745-3687 Transnational Research Core Moffitt Cancer Center Teresa d'Angelo Director of CTSI Research Services teresadangelo@ufl.edu CTSI Service Center University of Florida Luisa Dempere Program Director ademp@eng.ufl.edu (352) 392-6985 College of Engineering Research Service Centers University of Florida Ruth Didier Director Flow Cytometry/Confocal Microscopy ruth.didier@med.fsu.edu College of Medicine Biomedical Sciences Florida State University Dongtao (Ann) Fu Lab Manager fudo@pathology.ufl.edu (352) 273-7752 Molecular Pathology Core University of Florida Umesh Goli ugoli@chem.fsu.edu (850) 644-1697 Mass Spectrometry Lab Florida state University

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Joan Hare Research Scientist/Director Protein Expression Facility jhare@fsu.edu (850) 644-1421 Protein Expression Facility Florida State University Huan He hh06@fsu.edu IMB-TSL MS Florida State University Agnieszka Kasprzak Research Specialist agnieszka.kasprzak@moffitt.org Analytic Microscopy Core Moffitt Cancer Center Alecia Lapointe Program Coordinator fMRI Research at CoM Alecia.Lapointe@med.fsu.edu (508) 353-0148 College of Medicine FSU Steven Madore Associate Director for Science smadore@ufl.edu ICBR University of Florida Roger Mercer Director, Translational Science Laboratory roger.mercer@med.fsu.edu (850) 645-9598 Florida State University Steven Miller smiller@bio.fsu.edu FSU DNA Sequencing Facility FSU Claudius Mundoma Director, Physical Biochemistry Facility cmundoma@fsu.edu (850) 644-5147 Physical Biochemistry Facility Florida State University

Carlos Murphy IT Business Analyst murphy.carlos@gmail.com (850) 228-2213 Facilities FSU Antoinette Noel Core Research Facilities Tech I jeanie@ufl.edu (352) 272-8042 Monoclonal Antibody Core Interdisciplinary Center for Biotechnology Research University of Florida Nedka Panayotova nedpan@ufl.edu NextGenSeq ICBR UF Kristina Poduch poduch@bio.fsu.edu MCF FSU Cheryl Pye Researcher cfitch@neuro.fsu.edu Molecular Cloning Facility FSU Alberto Riva Associate Scientist ariva@ufl.edu ICBR Bioinformatics Core University of Florida Katherine Santostefano hankows@pathology.ufl.edu Center for Cellular Reprogramming University of Florida Margaret Seavy Manager, Analytical Laboratory, Biological Science seavy@bio.fsu.edu (850) 644-5444 Department of Biology Florida State University

Savita Shanker Associate Scientist/Scientific Director sshanker@ufl.edu (352) 273-8056 Sanger Sequencing Core UF Rakesh Singh Research Faculty I rakesh.singh@med.fsu.edu (850) 980-4551 Translational Science Laboratory FSU Vivek Somasundaram Undergraduate Student vts13@my.fsu.edu Florida State University Thayumanasamy Somasundaram X-Ray Facility Director tsomasundaram@fsu.edu (850) 644-6448 Institute of Molecular Biophysics Florida State University Duncan Sousa dsousa@fsu.edu Biological Science Imaging Resource FSU John Spear Scientific Research Specialist jms09z@fsu.edu (845) 656-1665 Biological Science Imaging Resource Florida State University Larry Tucker Vice President Business Development ltucker@metrohmusa.com (813) 316-4752 Metrohm USA Bert van de Burgt Director, Material Characterization Laboratory vandeburgt@chem.fsu.edu (850) 644-3468 Department of Chemistry & Biochemistry Florida State University

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Daniel Vera Director FSU Center for Genomics and Personalized Medicine vera@genomics.fsu.edu (850) 296-8372 FSU Florida State University Cynthia Vied Research Faculty cynthia.vied@med.fsu.edu (850) 645-8491 Translational Science Laboratory Florida State University Brian Washburn Lab Manager Hybridoma/Cloning washburn@bio.fsu.edu (850) 644-0575 Biological Science Florida State University

Leigh West Scientific Researcher westl@usf.edu (813) 974-6270 Protein Production/Cell Biology University of South Florida Center for Drug Discovery and Innovation Bonnie Wright Grants Analyst and Team Leader wright@psy.fsu.edu (850) 645-8192 Neuroscience & Psychology Florida State University Yanming Yang yyang4@fsu.edu Translational Science Lab Florida State University Michael Zawrotny mzawrotny@fsu.edu Computer Resources Facility IMB

FSU Shumin Zhang Research Specialist shumin.zhang@moffitt.org (813) 745-8501 Translational Research Core Moffitt Cancer Center Mei Zhang Biological Scientist mzhang14@ufl.edu Gene Expression & Genotyping University of Florida NIng Zhu Biological scientist nzhu@ufl.edu (352) 273-8060 Proteomics core ICBR" University of Florida

Florida Core Network Event 3 Program

Florida State University, Tallahassee, FL 32306 August 11, 2016 Third Annual Event

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NOTES

Florida Core Network Event 3 Program

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