José Bento Pereira Lima

Innovative Vector Control approaches:

Wolbachia

Transgenic mosquitoes

Pesticides, new molecules, Bacilos

First Regional Dengue Symposium

With 1.4 million cases throughout Brazil, dengue hits record

According to the latest epidemiological bulletin of dengue Ministry

of Health, were 1,463,776 probable cases of the disease recorded

from January 4 to 26 September in Brazil.

The previous record of 2013 was 1,452,489 people infected.

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vacine specific drugs Mosquito control

Products

7-10 days

eggs larvae and pupae adultos

Genilton J. Vieira / IOC Genilton J. Vieira / IOC

Life cycle

With visiting house to house in two months cyclesand in some areas the pending reaches 40%It is very difficult the vector control

Dengue mosquito - breeding

• Intracellular

bacteria

• Present in 20-

65% of all species

of insects,

including

mosquitoes that

bite humans.

WOLBACHIA

cytoplasmic incompatibility

X

X

X

X

0 20 40 60 80 100

Hatch rate %Hatching rate%

2011: Release of wMel in Yorkeys Knob and Gordonvale

Rio de JaneiroBrasil

GuangdongChina

Tri Nguyen Vietnam

CairnsAustralia

YogyakartaIndonésia

Gerenciamento do Projeto

National projects in progress

URCA TUBIACANGA

VALQUEIRE JURUJUBA

•Goal – evaluate RIDL technology (OX513A) in open field release

•Collaboration between Universidade de São Paulo and Moscamed Brasil

•Oxitec agreement – No bias in the evaluation

A field release validation for transgenic

Aedes aegypti population suppression

Program OverviewProduction -> Release -> Monitoring

Adult traps

Eggs produced

(4-6 million/week)

Rear release

generation

Separate Male

Pupae

Placed in release

device

Eggs -> Pupae

1 million/week

♂

Release males

Adults x 3 week

(0.5 to 1.5 million/week)

Monitoring

Hatch eggsEggs traps

Larvae screened

for presence of

Fluorescent

genetic marker

Mating

Itaberaba1400 houses

550 km²

Mandacaru600 houses

360 km²

~ 15 Km distance

Site Selection

PLOS Neglected Tropical Diseases | DOI:10.1371/journal.pntd.0003864 July 2, 2015

New Project – Jacobina - Bahia

80% 40%

Jacobina Suppression

Diretor da Vigilância Epidemiológica - Jacobina – José Alves Silva Junior“em 2012 Catuaba o IIP = 4,4%, Pedra Branca = 16,7%.......em 2014 Catuaba = 2,2% e Pedra Branca 2,2%, concluindo que nesse momento o importante é a prevenção”.

Aedes aegypti Production (UPAT)

Males for releases 1,5 million/week

● Actellic 300 CS was evaluated in Ivory Coast (Swiss TPH), Benin (LSHTM / CREC) and Tanzania(LSHTM / KCMC) study in cabin areas;

● Studies show a higher residual control than 6 months on surfaces such as cement and clay;

● organophosphate, pirimiphos-methyl, ideal for resistance management;

● New long-term formulation (microencapsulated suspension / CS).

New Products - adulticide

Mosquitoes come in contact withthe capsule on the treatedsurfaces: Capsule in mosquitolegs.

Capsules Actellic 300 CSAre intact after application: Capsules in cement

New Products - adulticide

New Products - adulticide

Bti PRESENTATIONS

mini-tabletes DT(tablete de uso direto)

granulado WG(grânulo dispersível em água)

0 cm 1 2 3 4 5 6

C1 C2 T A10

01 mini-tablete 6 g

50 L 100 L 300 L 500 L 10.000 L

New Products - larvicide

eficacy + than 60 days; choque effect in a few hours

sustentability no history of vector resistance!

safety potable water; no sensitizing/irritating; min. exposition

practicality doses and formules read to use; need no equipments

aplicability concentrated product; tolerates temperature variations

TECHNICAL REQUIREMENTS

16 C1 C2 T A

New Products - larvicide

New Products - larvicide

New Products - larvicide

No odorUsed for drinking waterProduct is under WHOPES

SumiLarv® 2MR is a mosquito larvicidaldevice and its active ingredient, pyriproxyfen is incorporated into polymer resin as anewest formulation “MR”

Spinosad

Dissemination of Pyriproxyfen

DENGUE - Amazônia

Contamination byinsecticide impregnated

Contamination of theaquatic environment

DENGUE - Amazônia

Results

larva muerta pupa muerta eclosion inhibicion

8%0%

92%

8%

18%

61%

21%

89%

1% 3%

96%

4%

% Mortality of larvae and pupae hatching inhibition of mosquitoes Before, During and After the Intervención with pyriproxifen

ANTES DURANTE

DESPUES

DENGUE - Amazônia

• Mosquitoes spread the pyriproxifen to ≈ 0.5 km (500 meters).

• 100% of the houses were infested with pyriproxifen and 94% of sentinel breeding.

• The pyriproxifen causes mortality of larvae and pupae.

• The mortality of larvae and pupae in sentinel breeding is higher as is closer to the pyriproxifen broadcasting stations.

DENGUE - Amazônia

MANACAPURU

DENGUE - Amazônia

DENGUE - Amazônia

Meses Aedes

aegyptiAedes

albopictus Limatus Culex Spp

Nov. 2013 1128 3155 185 37

Dez.2013 1556 5881 87 30

Jan.2014 1533 3154 119 7

Feb. 2014 82 741 130 9

Mar.2014 288 930 89 6

Before Abr.2014 224 695 183 4

Mai.2014 286 965 371 0

Jun.2014 341 677 76 0

Jul.2014 155 772 31 0

Ago.2014 87 587 2 0

Set.2014 109 1018 0 6

Out.2014 530 779 10 2

Dez.2014 543 1062 91 22

Jan.2015 363 1272 149 2

Feb.2015 238 542 131 0

Total 7463 22230 1654 125

Meses Aedes

aegyptiAedes

albopictus Limatus Culex Spp

Mar.2015 130 371 0 0

DURANTE Abr.2015 121 306 0 7

Mai.2015 65 130 0 0

Jun.2015 11 56 0 0

Jul.2015

Total 327 863 0 7

DENGUE - Amazônia

Monitoring and control in Itacoatiara - AM

0

10

20

30

40

50

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Positivity and average weekly eggs

Positivity Média+

New RNAi methods for vector control

dsRNA targeting

β-tubulin

Temephos

Increased toxicity to Temephos

Insect Molecular Biology (2013) 22(6), 648-658

dsRNA targeting

P-glycoprotein

Increased larvae mortality

Journal of Insect Science: 2013;13:69

dsRNA-treated larvae Phenotype in larvae

Parasites & Vectors 2015, 8:96

dsRNA targeting both testis genes and female sex

determinationReduced fertility

in males

Inhibition of female development

dsRNA-treated larvae Phenotype in adult mosquito

Rafael Freitas IOC - Fiocruz

Margareth Capurro – USP

Sergio Luz – Fiocruz Amazonas

Emerson Bernardes – Forest

Washington Ferreira – Syngenta

Haroldo Gomes -

Acknowledgment

OBRIGADO