Amir Mohammed Mohammed Ali Abdo

Contacts

Mobile number: 00447470132989 & 00201004914998‎ E-mail: Alamier@Ymail.com & ammabdo1@sheffield.ac.uk LinkedIn profile: https://uk.linkedin.com/in/amir-alsharabasy-71980630

Executive Summary

An experienced chemist and clinical biochemist with extensive experience in the protein purification and characterization under the GMP conditions, polymer modification and characterization as well as different biomacromolecules characterization. Key-strengths include: Dynamic management of the different activities in the lab to guarantee a smooth and safe working environment& engaging with the team to design the suitable dynamic plans for the recovery and purifying the resulting stream towards efficient downstream process for the desired molecules& strategic collaborative and integrated complete analysis of the 1ry and 2ry structures of samples with characterizing their properties and activity to optimize the manufacturing conditions and control the products’ characteristics & the ability to analyze the different blood components using different analytical techniques& controlled tailoring of biopolymer structures with different methods towards certain biomedical applications& and harnessing my academic research experience to create new solutions to problems during the Bioprocessing.

Expertise

*Chromatographic purification. *Chromatography-based analyses. *Protein structure analysis. *Measurement of macromolecules bioactivity. *Academic research experience. *Academic writing experience

*Polymers modification/ analysis *Working according to GMP. *Biochemical analyses. *Haematological assays. *Assembling new teams.

Career highlights

*Since Sep 2015 till Sep 2016: Studied biochemical engineering as a cornerstone point of change from the science-based previous experience and work to engineering. Performed my master’s project in bioprocess engineering concentrating on the designing of new purification systems for proteins within the downstream processing based on the aqueous two phase extraction with; analysed the properties of the extracted molecules and how they can be affected by the extraction conditions. Finished my master’s study. *Shared with Fujifilm Diosynth Company in the designing of a plan to analyse the source of bacterial contamination in a biomanufacturing process. Was responsible for analyzing the different accompanied causes and risks, how to analyze them and studying their impact on the whole business. The plans of the different competing teams were discussed and analyzed by technical experts from the company and business experts; our team received the greatest honour and highest grades among the others with an appreciation of my assessment strategy. From May 2012 till now: Worked as a research assistant of chemistry in the national center for radiation research and technology. Was working in the field of extraction and modification of biopolymers using chemical and radiation-based methods for further biomedical applications such as the designing of wound dressings; tested the efficiency of the wound dressings in vitro and vivo; worked as a member of the molecular biology lab with cooperation with the other colleagues in their work and dissertations. Finished my master’s study in biochemistry; published two papers and more manuscripts are waiting for publishing; started some work on bioactive glass synthesis for bone treatment applications.

*From Oct. 2009 to Sep.2015: Worked as a clinical chemistry analyst in a number of clinical labs in Egypt. Was the manager of a branch in three of the labs; performed an analysis of different blood components, including proteins, enzymes, hormones, viruses, elements and blood cells using different analytical techniques such as ELISA and PCR; worked as a Phlebotomist. Was able to work in harmony with the other members of lab to deliver the accurate results in time with ability to handle the different problems we used to face during our work.

Career History

Employer: The National center for radiation research and technology (NCRRT)/Egyptian atomic energy authority, Cairo, Egypt.

Position Research Assistant (Chemistry). Dates: May 2012 – now. I worked in the molecular biology lab with concentrating on the synthesis and modification of polymers and bioactive glasses for the biomedical applications *Carried out my master research experiments focusing on the application of radiation in preparing of biopolymers-based new wound dressings with the biomedical engineering principles. *Characterized the physicochemical properties of polymers and the different prepared hydrogels using SEM, TEM, GC, XRD, FTIR and UV spectroscopy and fluorescence spectroscopic techniques. *Demonstrated the efficacy of the designed dressings in vitro and in vivo with molecular biology as well as histological techniques. *Extracted, purified, and modified number of biopolymers with chemical and radiation methods in addition to the bioactive glass synthesis, modification and characterization. *Synthesized number of biomaterial-based composites in the form of hydrogels and rigid structures using Chemical and physical methods. *Shared in the organisation of scientific training courses in molecular biology between the NCRRT and the Middle Eastern Regional Radioisotope Centre for the Arab Countries, Giza, Egypt.

Employer: Nile Scan and labs, Giza, Egypt& Royal lab-Clinical Labs, Giza, Egypt& Cairo Scan-clinical Labs, Cairo, Egypt& Sanaa Abdel-shafy -Clinical Labs, Giza, Egypt.

Position Clinical Chemistry Analyst. Dates: Oct 2009 – Sept 2015. *Performed the analysis of different blood components including proteins, enzymes, hormones, viruses, elements and blood cells using different analytical techniques such as ELISA and PCR. *Worked as a Phlebotomist. *Was the manager of number of branches for facilitating the connection with the other branches in each lab, deliver the results in time and resolve any problems in the branch. Employer: Matrix for Scientific Services, Cairo, Egypt. Position Chemist for Water Analysis. Dates: June 2010 - Jan 2011. *Performed different tests for the analysis and detection of different elements in all water and soil types, and their concentrations. *Was responsible for the sampling, visited a number of international companies factories and understood the nature of their work, organization, and how the quality requirements and safety precautions are applied.

Education

Nov. 2016 Masters’ Degree in Biological and Bioprocess Engineering, Faculty of Engineering, Sheffield University, U.K. Project title: Alternatives to the PEG/phosphate aqueous biphasic protein extraction

systems

June 2014 Masters’ Degree in Science (Biochemistry), Faculty of Science, Helwan University, Egypt Project Title: Molecular biological studies to evaluate the treatment role of irradiated scaffolds in ulcers and wounds in rat skin.

June 2009 Bachelor in Science (Chemistry and Biochemistry), Faculty of Science, Mansoura University, Egypt (Grade: 1st Class Honors Degree (84.98%) – ranked 3rd in the class.

Training Course Attendance 1. Fujifilm Diosynth Biotechnologies, Billingham, U.K (April 2016): -The upstream and downstream processing -The designing and operating of bioreactors for 3D cell cultures. -Training on the designing of projects for manufacturing of monoclonal antibodies and the removal of contamination from the cell cultures. This was part of a collaboration between the Department of Chemical and Biological Engineering, Sheffield University and FUJIFILM Diosynth. 2. Training workshops held by GE healthcare in the Department of Chemical and Biological Engineering (April 2016): The downstream processing and protein purification using HPLC&GC 3. Royal Navy in Sheffield and the Portsmouth Harbour (March 2016): Engineering Leadership training program 4. The Egyptian atomic energy authority (EAEA), Nasr City, Cairo (March 2015): The chromatographic analytical techniques workshop 5. The Egyptian atomic energy authority (EAEA), Nasr City, Cairo (September 2014) The International Atomic Energy Agency radioprotection courses and usage of ionizing radiation. 6. Faculty of agriculture, Alazhar University, Cairo, Egypt (August 2011) The biostatistics principles, NCBI and gene banking.

Awards 1. NAM S&T Centre Research Training Fellowship for Developing Country Scientists (RTF-DCS) (2016-2017): from the Centre for Science and Technology of the NonAligned and Other Developing Countries to carry out a research study in the field of nanodrug delivery for 6 month in the Central Glass and Ceramics Research Institute (CGCRI), India. 2. Overseas Excellence Award for Master in Engineering (2015): From the Department of Chemical and Biological Engineering, Faculty of Engineering, Sheffield University. 3. Scientists for Next Generation (SNG) Grant for Masters in Science (2010): From the Egyptian Academy of Scientific Research and Technology, Egypt.

Publications: (My common name in publications: Amir Mohammed Alsharabasy) 1. Alsharabasy AM, Moghannem SA, El-Mazny WN. Physical preparation of alginate/chitosan

polyelectrolyte complexes for biomedical applications. Journal of Biomaterials Applications. 2016: 30(7): 1071–1079.

2. Eldegheidy E, Mahdy EM, El-Mezayen HA, Elmaghraby TK, Alsharabasy AM. Alterations in the functional properties of alginate/ chitosan PEC hydrogel film dressings following sterilization with gamma irradiation. Isotope and Radiation Research. 2014: 46(1): 25-37.

Two manuscripts have been accepted for publishing: -The first one concentrates on the biological application of the prepared wound dressing (from Ref. 1) and the in vivo assessment of its effects, and will be published in the Biomedical Physics and Engineering Express journal, IOPScience publishing. -The second one concentrates on the semi-synthesis of chitosan from shrimp shells through a new economical method, and will be published in the Journal of Polymer Science, Insight Medical Publishing.

Patent Alsharabasy AMMA. Alginate/Chitosan-based wound dressing and process for producing the same

and use thereof- patent filed March 2014. The Egyptian Patent Office – Patent ID (2014/402).

Special accomplishments:

Certified Journal Reviewer: Materials Science and Engineering: C, Journal of biomaterials Applications, American Journal of Bioscience and Bioengineering, International Journal of Biomedical Materials Research, Advances in Biochemistry journal, and Letters in Health & Biological Sciences. I have reviewed 91 full manuscripts and reviews since November 2014.

Editorial Board Member: American Journal of Bioscience and Bioengineering, International Journal of Biomedical Materials Research, Advances in Biochemistry journal, and Letters in Health & Biological Sciences.

Languages Arabic (native), English (Very good)/IELTS (6.5)), French (Good) Technology Skills SPSS program Matlab program Endnote program ChemDraw program Photoshop Autodesk Maya 3D animation software Address

Address Kafr Abu Zekry, Meniet Alnasr, Dakahliya, Egypt (Postcode: 35747)

Languages Arabic (mother tongue), English (Very good), French (Basic).

Nationality Egyptian