figure s1
DESCRIPTION
Cotton plug. Filled with NaOH pellets. Syringe tube. 0.45 μ m syringe filter. BG-11 medium & cells. Figure S1. 12 C (1.1% 13 C). 49.6% 13 C. 98% 13 C. 10.8% 13 C. 250. 150. 100. 75. Band selected for in-gel digestion. 50. MW (kDa). 37. 25. 20. 15. 10. Figure S2. - PowerPoint PPT PresentationTRANSCRIPT
Figure S1.
BG-11 medium& cells
0.45 μm syringe filter
Filled with NaOH pellets
Cotton plug
Syringe tube
Ban
d s
elec
ted
for
in-g
el d
iges
tion
MW
(k
Da)
250150100
75
50
37
25
15
10
20
12C
(1.
1% 13
C)
10.8
% 13
C
49.6
% 13
C
98%
13C
Figure S2.
Figure S3.
Figure S4.
Inte
nsit
y
960 980 1 000 1 020Raman shift (cm-1)
100
4.3
3.0
151
6.0
201
1.8
100
1.6
115
2.8
150
9.8
998
.1 114
6.2
150
3.5
995
.3 113
9.7
149
2.6
188
1.8
199
1.3
992
.1 113
3.3
148
8.0
112
5.0
988
.6
148
1.6
171
1.6
182
3.4
197
6.5
207
6.0
ν 3
Inte
nsit
y
1 100 1 120 1 140 1 160 1 180Raman shift (cm-1)
115
5.6
874
.7
100
4.9
127
4.0
151
6.0
201
1.8
115
2.8
100
1.7
150
9.8
114
6.9
998
.3
126
4.3
150
3.5
114
0.4
995
.0
126
1.2
149
2.6
188
1.8
199
1.3
113
2.9
991
.8
125
6.4
148
8.0
112
5.3
687
.6
854
.5
988
.4
125
3.1
148
1.6
158
4.3
171
1.6
182
3.4
187
5.8
197
6.5
207
6.0
ν 2
Inte
nsit
y
1 460 1 480 1 500 1 520 1 540Raman shift (cm-1)
151
6.8
874
.7
100
4.9
115
5.6
127
4.0
201
1.8
150
9.3
100
1.7
115
2.8
150
3.0
998
.3
114
6.9
126
4.3
149
3.3
995
.0
114
0.4
126
1.2
188
1.8
199
1.3
148
7.8
991
.8
113
2.9
125
6.4
148
0.9
687
.6
854
.5
988
.4
112
5.3
125
3.1
158
4.3
171
1.6
182
3.4
187
5.8
197
6.5
207
6.0
ν 1
Synechocystis sp. PCC 6803
Inte
nsit
y
960 980 1 000 1 020Raman shift (cm-1)
115
7.7
100
6.0 1
522.
3
203
9.6
994
.8
114
1.4
149
5.8
989
.5
112
8.4
148
4.8
ν 3In
tens
ity
1 100 1 120 1 140 1 160 1 180 1 200Raman shift (cm-1)
115
7.7
100
6.0
152
2.3
203
9.6
149
5.8
994
.8
114
0.7
112
8.2
989
.5
152
2.3
ν 2
Inte
nsit
y
1 460 1 480 1 500 1 520 1 540Raman shift (cm-1)
152
2.0
100
5.0
115
7.7
203
9.6
149
5.9
995
.1
114
1.4
148
4.9
990
.1
112
8.4
ν 1
Synechococcus elongatus PCC 7942
Raman shift (cm-1)
Inte
nsit
y
10.8
% 1
3 C
25.3
% 1
3 C
49.6
% 1
3 C
73.8
% 1
3 C
98%
13 C
12 C (with
natu
ral
13 C abun
danc
e
1.1%
)
49.6
% 1
3 C
98%
13 C
12 C (with
natu
ral
13 C abun
danc
e
1.1%
)
PCA result of RR spectra of Synechocystis sp. PCC 6803 single cells
A
12C (with natural 13C abundance 1.1%)
10.8% 13C
25.3% 13C
49.6% 13C
73.8% 13C
98% 13C
PCA result of RR spectra of Synechococcus elongatus PCC 7942 single cells
Figure S5.
B
12C (with natural 13C abundance 1.1%)
49.6% 13C
98% 13C
1.1% 13C
Theoretical isotopic cluster distributions of the peptide
13% 13C 54% 13C
Isotopic cluster distributions of the peptide
96% 13C
765.
4
765.
9
766.
4
765.50 766.50 767.50
766.
9
767.
4
m/z, Da
IntensityIntensity
m/z, Da m/z, Da m/z, Da
m/z, Da m/z, Da m/z, Da m/z, Da
765.
4
765.
9
766.
4
766.
9
767.
4
766 769 772
769.
476
9.9
773.
4
770.
477
0.9
771.
477
1.9
772.
477
2.9
768.
976
8.4
767.
976
7.4
766.
976
6.4
769.
4
773.
4
770.
477
0.9
771.
477
1.9
772.
477
2.9
768.
476
7.9
767.
476
6.9
766.
4
769.
9
765.
4
768.
9
765.
976
5.9
782.
5
777.
4
783.
578
4.5
785.
578
6.5
778.
4
781.
578
0.5
779.
5778 782 786
782.
5
777.
4
783.
578
4.5
785.
578
6.5
778.
4
781.
578
0.5
779.
5
797.
5
792.
5
795.
579
5.0
794.
579
4.0
793.
579
3.0
796.
079
6.5
797.
0
792 795
797.
5
792.
5
795.
079
4.5
794.
079
3.5
793.
0
796.
079
6.5
797.
0
795.
5
792.
079
1.5
Figure S6