figure 09: phage t4 life cycle - faculty.weber.edufaculty.weber.edu/coberg/4154...
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Figure 09: Phage T4 life cycle
Adapted from K. Thiel, Nat. Biotechnol. 22 (2004): 31-36.
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Figure 03B: Phage T4 plaques on a bacterial lawn
Part B © Ken Wagner/Visuals Unlimited
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λ can enter the ly+c or lysogenic cycle
Figure 8.28 Adapted from A. Campbell, Nat. Rev. Genet. 4 (2003): 471-‐477.
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Figure 9.12
RNA
ssDNA
dsDNA
MS2 ss ds φ6
φψ174 fd, M13
T3, T7
Mu Lambda T2, T4
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Figure 19: Map of Phage φX174
Adapted from an illustration by New England BioLabs, Inc.
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Figure 31: Electron micrograph of bacteriophage P1.
Photo courtesy of Michel Wurtz and the Biocenter at the University of Basel
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Figure 10.14
Transducing particle (contains donor cell DNA)
Phage
Donor cell
Phage DNA
Host DNA
Normal phage
Transduced recipient cell
Recipient cell
Lytic cycle
Transduction
Normal lytic events
Recipient infected by transducing particle
Homologous recombination
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Generalized transducing par+cles carry random
bacterial genes
Figure 8.32
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Figure 9.16 Temperate virus
Lytic pathway Lysogenic pathway
Induction
Viral DNA Host DNA
Cell (host)
Lysogenized cell
Attachment
Injection
Viral DNA replicates
Coat proteins synthesized; virus particles assembled
Viral DNA is integrated into host DNA
Lysis Cell division
Prophage
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Figure 9.18
Lambda genome
Host genes near attachment site
Host DNA
Cyclizes at cohesive ends
Site-specific nuclease creates staggered ends of phage and host DNA
Integration of lambda DNA and closing of gaps by DNA ligase
cos
att
cos
att
gal bio moa
gal bio moa
gal cos bio moa
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λ phage is a versa+le cloning vector
Figure 8.30
Nonessen+al gene region can be replaced by 12-‐22 kb of foreign DNA.