人工組織・センシング・細菌制御の新技術　～多様なバイオ産業応用を目指して～　New technologies for artificial organs, bio-sensing and bacterial control: toward various bioindustry applications

味覚の仕組みを模倣することで、バイオ試料の“味”を認識Recognition of the "taste" of biological samples by mimicking the mechanism of the gustatory system

（研究拠点　つくば／ AIST-Tsukuba）

冨田　峻介 健康医工学研究部門TOMITA Shunsuke Health and Medical Research Institute

バイオ試料の特性を蛍光パターンとして出力可能な分子アレイを開発

血清や細胞分泌物、腸内細菌叢のような複雑性バイオ試料の差異を判別

複雑で不明瞭なバイオ試料を分類・判別するための新規指標を提供

Development of molecular arrays for converting the sample characteristics into fluorescence patterns

Differentiation of complex biological samples such as serum, cell secretions, and gut microbiota

Providing new index for classification of complex and obscure biological samples

Fusion of biosensing and pattern recognition

バイオセンシングとパターン認識の融合

これまでに得られたバイオ試料評価の結果の例

開発した chemical tongue の模式図と材料および結果の例

Examples of biosample evaluation results obtained so far

Schematic illustration of the developed chemical tongue and examples of materials and a result

タンパク質

Score (1), 60.6%

Scor

e (2

), 26

.7%

TraPepMyoLysLipLacGIgGHRPHemGOX

GalFibConCatCarBSAlacAAntAmyHSA

-10

-50

5

Scor

e (3

), 5.

8%

-10-50

510

Score (2), 9.5%

-10

0

10

20

Scor

e (1

), 80

.3%

HL60P388

Raji

HuH7

HeLa

哺乳類細胞

A431

MCF7

TIGMG63

MDA

Score (1), 76.7%

Scor

e (2

), 18

.7%

Native

Denatured

Aggregated

Antibody AAntibody B

Young Senescent

Score (1)

Scor

e (2

)Sc

ore

(3)

細胞老化 抗体劣化

細胞分化 哺乳類血清

Score (1), 93.6%

Scor

e (2

), 6.

4% Bovine

Human

HorseRabbit

Fetal bovineUndiff.

Osteo. Adipo.

Score (1), 80.5%

Scor

e (2

), 19

.5%

R =

R =

NH3+ OH

NH

O

R =

NHO

NH

R

O

547113NH

OO

N H

NH

O

NH3+

NH3+

認識ユニット

出力ユニットR

S OO

N

NO

N

S NOO

NH

O OHO

COOH

S

ACS AMI, 2017ACS Sens., 2019

ACS AMI, 2019

R =

分析試料の応答パターン

模倣

味覚

パターン認識

Score (1), 60.6%

Scor

e (2

), 26

.7%

TraPepMyoLysLip

LacGIgGHRPHemGOX

GalFibConCatCar

BSAlacAAntAmyHSA

例︓20 種類のタンパク質の識別

腸内細菌

腸内細菌叢

Firmicutes

Actinobacteria

Bacteroidetes

Score (1), 31.2%

Scor

e (2

), 26

.8%

Proteo-bacteria

Score (1)

HealthyInsomnia

Figure 1

Figure 2

R =

R =

NH3+ OH

NH

O

R =

NHO

NH

R

O

547113NH

OO

N H

NH

O

NH3+

NH3+

Recognition unit

Transduction unitR

S OO

N

NO

N

S NOO

NH

O OHO

COOH

S

ACS AMI, 2017ACS Sens., 2019

ACS AMI, 2019

R =

Response patternsof samples

Mimic

The taste system

Pattern-recognition

Score (1), 60.6%

Scor

e (2

), 26

.7%

TraPepMyoLysLip

LacGIgGHRPHemGOX

GalFibConCatCar

BSAlacAAntAmyHSA

Ex. Identification of 20 different proteins

Figure 1 (English)

Proteins

Score (1), 60.6%

Scor

e (2

), 26

.7%

TraPepMyoLysLipLacGIgGHRPHemGOX

GalFibConCatCarBSAlacAAntAmyHSA

-10

-50

5

Scor

e (3

), 5.

8%

-10-50

510

Score (2), 9.5%

-10

0

10

20

Scor

e (1

), 80

.3%

HL60P388

Raji

HuH7

HeLa

Mammalian cells

A431

MCF7

TIGMG63

MDA

Score (1), 76.7%

Scor

e (2

), 18

.7%

Native

Denatured

Aggregated

Antibody AAntibody B

Young Senescent

Score (1)

Scor

e (2

)Sc

ore

(3)

Cell senescence Antibody degradation

Cell differentiation Mammalian sera

Score (1), 93.6%

Scor

e (2

), 6.

4% Bovine

Human

HorseRabbit

Fetal bovineUndiff.

Osteo. Adipo.

Score (1), 80.5%

Scor

e (2

), 19

.5%

Gut-derived bacteria

Gut microbiome

Firmicutes

Actinobacteria

Bacteroidetes

Score (1), 31.2%

Scor

e (2

), 26

.8%

Proteo-bacteria

Score (1)

HealthyInsomnia

Figure 2 (English)タンパク質

Score (1), 60.6%

Scor

e (2

), 26

.7%

TraPepMyoLysLipLacGIgGHRPHemGOX

GalFibConCatCarBSAlacAAntAmyHSA

-10

-50

5

Scor

e (3

), 5.

8%

-10-50

510

Score (2), 9.5%

-10

0

10

20

Scor

e (1

), 80

.3%

HL60P388

Raji

HuH7

HeLa

哺乳類細胞

A431

MCF7

TIGMG63

MDA

Score (1), 76.7%

Scor

e (2

), 18

.7%

Native

Denatured

Aggregated

Antibody AAntibody B

Young Senescent

Score (1)

Scor

e (2

)Sc

ore

(3)

細胞老化 抗体劣化

細胞分化 哺乳類血清

Score (1), 93.6%

Scor

e (2

), 6.

4% Bovine

Human

HorseRabbit

Fetal bovineUndiff.

Osteo. Adipo.

Score (1), 80.5%

Scor

e (2

), 19

.5%

R =

R =

NH3+ OH

NH

O

R =

NHO

NH

R

O

547113NH

OO

N H

NH

O

NH3+

NH3+

認識ユニット

出力ユニットR

S OO

N

NO

N

S NOO

NH

O OHO

COOH

S

ACS AMI, 2017ACS Sens., 2019

ACS AMI, 2019

R =

分析試料の応答パターン

模倣

味覚

パターン認識

Score (1), 60.6%

Scor

e (2

), 26

.7%

TraPepMyoLysLip

LacGIgGHRPHemGOX

GalFibConCatCar

BSAlacAAntAmyHSA

例︓20 種類のタンパク質の識別

腸内細菌

腸内細菌叢

Firmicutes

Actinobacteria

Bacteroidetes

Score (1), 31.2%

Scor

e (2

), 26

.8%

Proteo-bacteria

Score (1)

HealthyInsomnia

Figure 1

Figure 2

R =

R =

NH3+ OH

NH

O

R =

NHO

NH

R

O

547113NH

OO

N H

NH

O

NH3+

NH3+

Recognition unit

Transduction unitR

S OO

N

NO

N

S NOO

NH

O OHO

COOH

S

ACS AMI, 2017ACS Sens., 2019

ACS AMI, 2019

R =

Response patternsof samples

Mimic

The taste system

Pattern-recognition

Score (1), 60.6%

Scor

e (2

), 26

.7%

TraPepMyoLysLip

LacGIgGHRPHemGOX

GalFibConCatCar

BSAlacAAntAmyHSA

Ex. Identification of 20 different proteins

Figure 1 (English)

Proteins

Score (1), 60.6%

Scor

e (2

), 26

.7%

TraPepMyoLysLipLacGIgGHRPHemGOX

GalFibConCatCarBSAlacAAntAmyHSA

-10

-50

5

Scor

e (3

), 5.

8%

-10-50

510

Score (2), 9.5%

-10

0

10

20

Scor

e (1

), 80

.3%

HL60P388

Raji

HuH7

HeLa

Mammalian cells

A431

MCF7

TIGMG63

MDA

Score (1), 76.7%

Scor

e (2

), 18

.7%

Native

Denatured

Aggregated

Antibody AAntibody B

Young Senescent

Score (1)

Scor

e (2

)Sc

ore

(3)

Cell senescence Antibody degradation

Cell differentiation Mammalian sera

Score (1), 93.6%

Scor

e (2

), 6.

4% Bovine

Human

HorseRabbit

Fetal bovineUndiff.

Osteo. Adipo.

Score (1), 80.5%

Scor

e (2

), 19

.5%

Gut-derived bacteria

Gut microbiome

Firmicutes

Actinobacteria

Bacteroidetes

Score (1), 31.2%

Scor

e (2

), 26

.8%

Proteo-bacteria

Score (1)

HealthyInsomnia

Figure 2 (English)

本研究の一部は、JSPS 科研費 20H02774 および 17H04884 の助成を受けたものです。