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Fapas ® Food Microbiology (FEPAS ® ) Proficiency Testing Programme 2016

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Page 1: (FEPAS Proficiency Testing Programme 2016 - … · Lactic Acid Bacteria 12 ... Proficiency Testing in Food Microbiology. ... environmental swabs for detection and enumeration and

Fapas® Food Microbiology (FEPAS®) Proficiency Testing Programme

2016

Page 2: (FEPAS Proficiency Testing Programme 2016 - … · Lactic Acid Bacteria 12 ... Proficiency Testing in Food Microbiology. ... environmental swabs for detection and enumeration and

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Contents Who We Are 3

Fapas® Proficiency Tests 4

Enumeration Tests 5

Aerobic Plate Count 5

Bacillus cereus 6

Clostridium perfringens 7

Coagulase Positive Staphylococci 8

Coliforms 9

Enterobacteriaceae 10

Enterococci 11

Escherichia coli 11

Lactic Acid Bacteria 12

Listeria monocytogenes 12

Yeasts & Moulds 13

Detection Tests 14

Campylobacter spp. 14

Cronobacter (Enterobacter) sakazakii 14

Escherichia coli O157:H7(1) 15

Listeria monocytogenes/Listeria spp. 16

Salmonella spp. 17

Vibrio parahaemolyticus 18

Unknown Pathogen Swab 19

Appendix 1: Distribution Details 20

Ordering Information 25

Technical Information 26

Protocols 26

Other technical documents 26

ISO Accreditation 26

Agent Information 27

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Fapas® Food Microbiology

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Who We Are Fapas® is the leading global provider of proficiency testing schemes, quality control samples and reference materials in the food sector, offering products throughout the year.

Proficiency testing is an independent check of your laboratory procedures providing you with a completely confidential assessment of your capability. Not only does participation in the relevant testing schemes allow you to demonstrate your systems and the technical ability of your staff, it will also help you gain and maintain ISO/17025 accreditation.

Established in 1990, we are an experienced, accredited provider of proficiency tests for the food and water, environmental chemistry and microbiology sectors. Taking part in our proficiency tests provides you with the confidence in your laboratory equipment, methods and staff, and assurance that you are delivering the quality results required by your customers.

We provide our participants with a confidential service that allows you to participate at a level that suits you; there is no minimum number of proficiency tests that you must take part in each year. Our system provides an easy reporting facility via our website and our comprehensive reports, which receive rigorous statistical analysis, contain method comparisons.

We also offer quality control samples and reference materials from selected food chemistry proficiency tests which can be purchased easily through our website throughout the year.

Our four Fapas® proficiency testing schemes cover food chemistry, food microbiology, GM and water and environmental analysis.

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Fapas® Proficiency Tests Proficiency Testing in Food Microbiology

Proficiency testing is an essential part of laboratory quality procedures. Taking part gives you confidence in your laboratory equipment, methods and staff, and assurance that you are delivering the quality results demanded by your customers.

Fapas® launched its food microbiology proficiency tests in 1997 as a proficiency testing scheme to assess the performance of laboratories carrying out microbiological examinations in the food area. Fapas® is an international proficiency test provider; the scheme has more than 500 participants in over 100 countries. Participants can monitor performance on a global scale. Our proficiency test samples are real food samples with target organisms for detection or enumeration. Our test materials are designed to simulate real laboratory samples with varying levels of target organisms and background flora, they provide challenging, high quality and innovative proficiency tests. Food matrices include beef, chicken, fish, soft cheese, milk powder, salad and rice. In addition we have environmental swabs for detection and enumeration and a proficiency test for an Unknown organism in a food matrix. Target organisms include pathogens, indicator micro-organisms and spoilage organisms.

There is a flexible programme of 10 distributions a year to meet your laboratories quality systems. Samples are sent by courier worldwide in a controlled environment with detailed instructions.

Results from each proficiency test receive rigorous statistical analysis, ensuring you have clear feedback on your performance. Comprehensive reports give information on microbiological methods used by other participants.

Participation in Fapas® is easy. Arrangements can be made through our network of agents, or via our website, www.fapas.com.

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Enumeration Tests For each proficiency test in enumeration, participants will receive 1 sample equivalent to 10 g after reconstitution (or 1 swab).

Aerobic Plate Count dispatch

date test details

dd/mm/yyyy code matrix analyte approx. quantity

29/02/2016 M210e13 beef Bacillus cereus & Aerobic Plate Count

equivalent to 10 g

11/04/2016 M211e05 beef Enterobacteriaceae & Aerobic Plate Count

equivalent to 10 g

11/04/2016 M211e09 milk powder Aerobic Plate Count equivalent to 10 g

09/05/2016 M212e13 milk powder Bacillus cereus & Aerobic Plate Count

equivalent to 10 g

06/06/2016 M213e09 swab Aerobic Plate Count 1 x swab

05/09/2016 M215e09 milk powder Aerobic Plate Count equivalent to 10 g

31/10/2016 M217e09 chicken Aerobic Plate Count equivalent to 10 g

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Bacillus cereus dispatch

date test details

dd/mm/yyyy code matrix analyte approx. quantity

01/02/2016 M209e12 cooked rice Bacillus cereus equivalent to 10 g

29/02/2016 M210e13 beef Bacillus cereus & Aerobic Plate Count

equivalent to 10 g

09/05/2016 M212e13 milk powder Bacillus cereus & Aerobic Plate Count

equivalent to 10 g

05/09/2016 M215e12 cooked rice Bacillus cereus equivalent to 10 g

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Clostridium perfringens dispatch

date test details

dd/mm/yyyy code matrix analyte approx. quantity

09/05/2016 M212e06 milk powder Clostridium perfringens equivalent to 10 g

04/07/2016 M214e06 beef Clostridium perfringens equivalent to 10 g

03/10/2016 M216e06 milk powder Clostridium perfringens equivalent to 10 g

28/11/2016 M218e06 beef Clostridium perfringens equivalent to 10 g

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Coagulase Positive Staphylococci dispatch

date test details

dd/mm/yyyy code matrix analyte approx. quantity

01/02/2016 M209e14 beef Coagulase Positive Staphylococci

equivalent to 10 g

11/04/2016 M211e14 beef Coagulase Positive Staphylococci

equivalent to 10 g

06/06/2016 M213e14 milk powder Coagulase Positive Staphylococci

equivalent to 10 g

31/10/2016 M217e14 milk powder Coagulase Positive Staphylococci

equivalent to 10 g

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Coliforms dispatch

date test details

dd/mm/yyyy code matrix analyte approx. quantity

29/02/2016 M210e18 milk powder Coliforms equivalent to 10 g

09/05/2016 M212e18 beef Coliforms equivalent to 10 g

05/09/2016 M215e18 milk powder Coliforms equivalent to 10 g

28/11/2016 M218e04 beef Enterobacteriaceae, Coliforms & Escherichia coli

equivalent to 10 g

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Enterobacteriaceae dispatch

date test details

dd/mm/yyyy code matrix analyte approx. quantity

01/02/2016 M209e03 beef Enterobacteriaceae equivalent to 10 g

11/04/2016 M211e05 beef Enterobacteriaceae & Aerobic Plate Count

equivalent to 10 g

05/09/2015 M215e03 salad Enterobacteriaceae equivalent to 10 g

28/11/2016 M218e03 milk powder Enterobacteriaceae equivalent to 10 g

28/11/2016 M218e04 beef Enterobacteriaceae, Coliforms & Escherichia coli

equivalent to 10 g

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Enterococci dispatch

date test details

dd/mm/yyyy code matrix analyte approx. quantity

03/10/2016 M216e19 beef Enterococci equivalent to 10 g

Escherichia coli dispatch

date test details

dd/mm/yyyy code matrix analyte approx. quantity

09/05/2016 M212e10 beef Escherichia coli equivalent to 10 g

31/10/2016 M217e10 milk powder Escherichia coli equivalent to 10 g

28/11/2016 M218e04 beef Enterobacteriaceae, Coliforms & Escherichia coli

equivalent to 10 g

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Lactic Acid Bacteria dispatch

date test details

dd/mm/yyyy code matrix analyte approx. quantity

03/10/2016 M216e01 beef Lactic acid bacteria equivalent to 10 g

Listeria monocytogenes dispatch

date test details

dd/mm/yyyy code matrix analyte approx. quantity

11/04/2016 M211e02 chicken Listeria monocytogenes equivalent to 10 g

03/10/2016 M216e02 smoked fish Listeria monocytogenes equivalent to 10 g

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Yeasts & Moulds dispatch

date test details

dd/mm/yyyy code matrix analyte approx. quantity

11/04/2016 M211e15 flour Yeasts & Moulds equivalent to 10 g

06/06/2016 M213e15 beef Yeasts & Moulds equivalent to 10 g

04/07/2016 M214e15 flour Yeasts & Moulds equivalent to 10 g

28/11/2016 M218e15 flour Yeasts & Moulds equivalent to 10 g

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Detection Tests For each proficiency test in detection participants will receive 2 samples, each equivalent to 25g after reconstitution.

Campylobacter spp.

* Please note that both of the marked chicken tests will also include a trial enumeration sample, free of charge.

Cronobacter (Enterobacter) sakazakii

For this proficiency test participants will receive 2 samples each equivalent to 10 g after reconstitution.

dispatch date

test details

dd/mm/yyyy code matrix analyte approx. quantity

09/05/2016 M212d17* chicken Campylobacter spp. equivalent to 2 x 25 g + 1 x enumeration sample

05/09/2016 M215d17 milk powder Campylobacter spp. equivalent to 2 x 25 g

31/10/2016 M217d17* chicken Campylobacter spp. equivalent to 2 x 25 g + 1 x enumeration sample

dispatch date

test details

dd/mm/yyyy code matrix analyte approx. quantity

29/02/2016 M210d20 infant formula Cronobacter (Enterobacter) sakazakii

equivalent to 2 x 10 g

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Escherichia coli O157:H7(1)

(1) The strain of Escherichia coli O157:H7 used in our samples is atoxigenic i.e. it does not produce a toxin. It has eae genes but the genes for VT1/VT2 and STX1/STX2 are absent.

dispatch date

test details

dd/mm/yyyy code matrix analyte approx. quantity

11/04/2016 M211d11 salad Escherichia coli O157:H7(1) equivalent to 2 x 25 g

06/06/2016 M213d11 beef Escherichia coli O157:H7(1) equivalent to 2 x 25 g

31/10/2016 M217d11 beef Escherichia coli O157:H7(1) equivalent to 2 x 25 g

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Listeria monocytogenes/Listeria spp.

dispatch date

test details

dd/mm/yyyy code matrix analyte approx. quantity

01/02/2016 M209d02 beef Listeria monocytogenes/ Listeria spp.

equivalent to 2 x 25 g

29/02/2016 M210d02 sponge swab Listeria monocytogenes/ Listeria spp.

2 x swab

09/05/2016 M212d02 soft cheese Listeria monocytogenes/ Listeria spp.

equivalent to 2 x 25 g

06/06/2016 M213d02 chicken Listeria monocytogenes/ Listeria spp.

equivalent to 2 x 25 g

04/07/2016 M214d02 milk powder Listeria monocytogenes/ Listeria spp.

equivalent to 2 x 25 g

28/11/2016 M218d02 chicken Listeria monocytogenes/ Listeria spp.

equivalent to 2 x 25 g

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Salmonella spp.

dispatch date

test details

dd/mm/yyyy code matrix analyte approx. quantity

01/02/2016 M209d07 cocoa powder Salmonella spp. equivalent to 2 x 25 g

29/02/2016 M210d071 chicken Salmonella spp. equivalent to 2 x 25 g

29/02/2016 M210d072 sponge swabs Salmonella spp. 2 x swab

11/04/2016 M211d07 egg Salmonella spp. equivalent to 2 x 25 g

09/05/2016 M212d07 chicken Salmonella spp. equivalent to 2 x 25 g

06/06/2016 M213d07 salad Salmonella spp. equivalent to 2 x 25 g

04/07/2016 M214d07 herbs Salmonella spp. equivalent to 2 x 25 g

05/09/2016 M215d071 milk powder Salmonella spp. equivalent to 2 x 25 g

05/09/2016 M215d072 ground pepper Salmonella spp. equivalent to 2 x 25 g

03/10/2016 M216d071 animal feed Salmonella spp. equivalent to 2 x 25 g

03/10/2016 M216d072 chocolate Salmonella spp. equivalent to 2 x 25 g

31/10/2016 M217d07 milk powder Salmonella spp. equivalent to 2 x 25 g

28/11/2016 M218d071 beef Salmonella spp. equivalent to 2 x 25 g

28/11/2016 M218d072 salad with sprouting seeds

Salmonella spp. equivalent to 2 x 25 g

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Vibrio parahaemolyticus

dispatch date

test details

dd/mm/yyyy code matrix analyte approx. quantity

29/02/2016 M210d21 fish Vibrio parahaemolyticus equivalent to 2 x 25 g

04/07/2016 M214d21 fish Vibrio parahaemolyticus equivalent to 2 x 25 g

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Unknown Pathogen Swab This proficiency test is designed to challenge participants’ ability to examine a swab for an unknown pathogen. Participants will receive two sponge swabs; one of which will contain a pathogen associated with food. Participants will be asked to detect and identify the pathogen present. You may use whichever microbiological examination(s) you consider to be the most appropriate for the analysis.

dispatch date

test details

dd/mm/yyyy code matrix analyte approx. quantity

01/02/2016 M209d30 swab Unknown Pathogen 2 x swab

04/07/2016 M214d30 swab Unknown Pathogen 2 x swab

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Appendix 1: Distribution Details Beef Matrices

Distribution Number and Date of Dispatch

209 210 211 212 213 214 215 216 217 218

Enumeration Tests 01/02/16 29/02/16 11/04/16 09/05/16 06/06/16 04/07/16 05/09/16 03/10/16 31/10/16 28/11/16

Bacillus cereus/Aerobic Plate Count in beef

M210e13

Clostridium perfringens in beef M214e06 M218e06

Coagulase Positive Staphylococci in beef

M209e14 M211e14

Coliforms in beef M212e18

Enterobacteriaceae / Aerobic Plate Count in beef

M211e05

Enterobacteriaceae in beef M209e03

Enterobacteriaceae / Coliforms / Escherichia coli in beef

M218e04

Enterococci in beef M216e19

Escherichia coli in beef M212e10

Lactic Acid Bacteria in beef M216e01

Yeasts and Moulds in beef M213e15

Detection Tests

Escherichia coli O157:H7(1) in beef M213d11 M217d11

Listeria monocytogenes /Listeria spp. in beef

M209d02

Salmonella spp. in beef M218d071

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Chicken and Fish Matrices

Distribution Number and Date of Dispatch

209 210 211 212 213 214 215 216 217 218

Enumeration Tests 01/02/16 29/02/16 11/04/16 09/05/16 06/06/16 04/07/16 05/09/16 03/10/16 31/10/16 28/11/16

Aerobic Plate Count in chicken M217e09

Listeria monocytogenes in chicken M211e02 M217e02

Listeria monocytogenes in smoked fish M216e02

Detection Tests

Campylobacter spp. in chicken (includes trial enumeration sample, free of charge)

M212d17 M217d17

Listeria monocytogenes /Listeria spp. in chicken

M213d02 M218d02

Salmonella spp. in chicken M210d071 M212d07

Vibrio parahaemolyticus in fish M210d21 M214d21

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Milk Cheese and Egg Matrices

Distribution Number and Date of Dispatch

209 210 211 212 213 214 215 216 217 218

Enumeration Tests 01/02/16 29/02/16 11/04/16 09/05/16 06/06/16 04/07/16 05/09/16 03/10/16 31/10/16 28/11/16

Aerobic Plate Count in milk powder M211e09 M215e09

Bacillus cereus / Aerobic Plate Count in milk powder

M212e13

Clostridium perfringens in milk powder M212e06 M216e06

Coagulase Positive Staphylococci in milk powder

M213e14 M217e14

Coliforms in milk powder M210e18 M215e18

Enterobacteriaceae in milk powder M218e03 Escherichia coli in milk powder M217e10

Detection Tests

Campylobacter spp. in milk powder M215d17

Listeria monocytogenes /Listeria spp. in milk powder

M214d02

Listeria monocytogenes /Listeria spp. in soft cheese

M212d02

Salmonella spp. in egg (dried) M211d07 Salmonella spp. in milk powder M215d071 M217d07

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Miscellaneous Matrices Distribution Number and Date of Dispatch

209 210 211 212 213 214 215 216 217 218

Enumeration Tests 01/02/16 29/02/16 11/04/16 09/05/16 06/06/16 04/07/16 05/09/16 03/10/16 31/10/16 28/11/16

Aerobic Plate Count on cotton swab M213e09

Bacillus cereus in cooked rice M209e12 M215e12

Enterobacteriaceae in salad M215e03

Yeasts and Moulds in flour M211e15 M214e15 M218e15

Detection Tests

Cronobacter (Enterobacter) sakazakii in infant formula (2)

M210d20

Escherichia coli O157:H7(1) in salad M211d11

Listeria monocytogenes /Listeria spp.on sponge swabs(3)

M210d02

Salmonella spp. in animal feed M216d071

Salmonella spp. in chocolate M216d072

Salmonella spp. in cocoa powder M209d07

Salmonella spp. in ground pepper M215d072

Salmonella spp. in herbs M214d07

Salmonella spp. in salad M213d07

Salmonella spp. in salad with sprouting seeds

M218d072

Salmonella spp. on sponge swabs(3) M210d072

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Miscellaneous Matrices (continued) Distribution Number and Date of Dispatch

209 210 211 212 213 214 215 216 217 218

Detection Tests 01/02/16 29/02/16 11/04/16 09/05/16 06/06/16 04/07/16 05/09/16 03/10/16 31/10/16 28/11/16

Unknown pathogen on swab M209d30 M214d30

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Ordering Information Notes:

a) The dispatch date shown is our planned dispatch date. You will be notified by email if any of the tests you order are delayed or cancelled for any reason. The majority of test close dates are 17 days after dispatch.

b) Registrations for proficiency tests in the FEPAS Programme will close 28 days before the start date of the test.

c) Indicate a contact name and email address against each test you order, otherwise the name given will be used.

d) The approximate quantity of test material we will supply is given for each test. If your method needs more material please indicate the number of extra test materials you require. There is a charge for additional test materials.

e) The purchase of extra test materials does not entitle you to receive an extra performance assessment. You must place multiple orders for the test if you require this service.

f) Test materials are dispatched by normal post unless courier service is requested. Please contact FAPAS for prices of sending test materials by courier.

Additional notes: i. Dispatch

a) All samples are dispatched by courier to reduce the time taken for the sample to reach your laboratory. These are packed with ice blocks to prevent the sample undergoing large temperature fluctuations in transit but are not transported in refrigerated vehicles. Even if your sample does not arrive refrigerated it is still acceptable for use as it will have been chilled for the majority of its journey.

b) Please contact us if your courier dispatch has not arrived within 4 days. c) The tracking number of your courier consignment will be sent to the email address given for the sample contact

and the delivery contact for that test. The message will indicate how to track the consignment. d) Airway Bill Numbers are also available from the participant’s secure pages on the FAPAS® website. e) It is the responsibility of a participant to monitor the progress of their courier dispatch. f) Our packages are shipped as category B (UN3373).

ii. Import / Customs a) We are not responsible for damage or loss of test materials due to problems in customs or for import

fees. b) If you require special import permits for importing certain types of test material into your country please

inform us at least 3 weeks prior to dispatch date. There is a charge for this service. c) Phytosanitary certificates can be provided for plant based products where appropriate. There is a charge

for this service. iii. Results

a) Participants generally have 17 days to return results after a test material is dispatched. b) Result submission will only be allowed via the FAPAS website.

iv. Reports a) The cost of the test includes access to an electronic copy of the report for the person placing the order

and designated sample contact. b) Reports are normally available on our website within 25 days of the closing date of the test. c) The reports are in PDF format and are secured by a digital signature. Adobe Reader version 7 or greater

is required to open these files.

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Technical Information

Protocols

• Protocol part 1: Generic [English] [Espanol]

• Protocol part 3: Fapas® Food Microbiology [English] [Espanol]

Other technical documents

• Example Report

• On line results submission instructions [English] [Espanol]

• Terms & Conditions

ISO Accreditation

The Fapas® proficiency testing schemes are accredited by UKAS, Proficiency Testing Provider No. 0009.

• UKAS Accreditation Certificate

This accreditation confirms that we comply with the requirements of International Standard ISO/IEC 17043:2010.

In addition, Fera is accredited by other external bodies to other internationally recognised standards including ISO 9001:2008.

• Fera's Quality Documentation

Fapas (and other proficiency testing schemes) does not award accreditation. That is the responsibility of national accreditation bodies. A list of national and international accreditation bodies can be found at www.fasor.com/iso25. Results of proficiency testing are used by laboratory accreditation bodies as part of the process to assess the ability of laboratories to perform analytical tests for which accreditation is required.

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Agent Information FAPAS has a network of Agents to help you locally. Please contact your nearest office:

Argentina Phone: +5411-4701-6262 E-mail: [email protected] Armenia Phone: +7 495 707 28 68 E-mail: [email protected] Austria Phone: +49 40 49294 2930 E-mail: [email protected] Australia Phone: +61 (0)3 9416 0093 E-mail: [email protected] Azerbaijan Phone: +7 495 707 28 68 E-mail: [email protected] Belgium Phone: +32 2 736 62 18 E-mail: [email protected] Belarus Phone: +7 495 707 28 68 E-mail: [email protected] China (Shanghai): Phone: + 86 13311603693 E-mail: [email protected] China (Beijing) Phone: +86-10-88026887 E-mail: [email protected] Egypt Phone: +202 0114 15 215 26 E-mail: [email protected] Estonia Phone: +7 495 707 28 68 E-mail: [email protected] France Phone: +33 478643200 E-mail: [email protected] Georgia Phone: +7 495 707 28 68 E-mail: [email protected] Germany Phone: +49 40 49294 2930 E-mail: [email protected] Greece Phone: +30-210-2934745 E-mail: [email protected] India Phone: +91 40 29805251 E-mail: [email protected]

Indonesia Phone: +62 811824811 E-mail: [email protected] Israel Phone: +972572400503 E-mail: [email protected] Italy Phone: + 39 02 89540225 E-mail: giovanna.lampis@ starecotronics.it Japan Phone: +81 35211 1807 E-mail: [email protected] Kazakhstan Phone: +7 495 707 28 68 E-mail: [email protected] Korea: Phone: +81 2529 2282 E-mail: [email protected] Kyrgyzstan Phone: +7 495 707 28 68 E-mail: [email protected] Latvia Phone: +7 495 707 28 68 E-mail: [email protected] Lithuania Phone: +7 495 707 28 68 E-mail: [email protected] Malaysia Phone: +603-5122 5108 E-mail: [email protected] Moldova Phone: +7 495 707 28 68 E-mail: [email protected] Philippines Phone: +6324410430 E-mail: [email protected] Poland Phone: +48 61 868 6272 Email: [email protected] Portugal Phone: +351 22 996 20 69 E-mail: [email protected] Russia Phone: +7 495 707 28 68 E-mail: [email protected] South Africa Phone: +27114444330 E-mail: [email protected]

Spain Phone: +34 91.504.66.02 E-mail: [email protected] Switzerland Phone: +49 40 49294 2930 E-mail: [email protected] Taiwan Phone: +886228484509 E-mail: [email protected] Tajikistan Phone: +7 495 707 28 68 E-mail: [email protected] Thailand Phone: +6628817404 E-mail: [email protected] Turkey Phone: + 90 232 464 8006 E-mail: [email protected] Turkmenistan Phone: +7 495 707 28 68 E-mail: [email protected] Ukraine Phone: +7 495 707 28 68 E-mail: [email protected] Uzbekistan Phone: +7 495 707 28 68 E-mail: [email protected] United Arab Emirates Phone: +971-4-2852211 E-mail: [email protected] Vietnam Phone: +84 8 3726 0440 E-mail: [email protected] United Kingdom FAPAS Fera Science Ltd. (Fera) National Agri-Food Innovation Campus Sand Hutton York YO41 1LZ UK Tel: +44 (0)1904 462100 Fax: +44 (0)1904 500440 [email protected] www.fapas.com © Copyright Fera Science Ltd. (Fera) 2015. All rights reserved.