Extraction Of Polyphenol From Pomegranate

Introduction Polyphenols: Phenolic compounds are natural antioxidants present in plants which are important to human health.

  Benefits of Polyphenols: Prevent Cardiovascular Disease Reduce Inflammation Lower The Incidence of Cancers Diabetes Mellitus Reduce Rates of Mutagenesis Reduce Obesity Reduce Hypertension

MATERIALS AND METHODS

Pomegranate was collected and grinded.

Grinded powder was packed in air tight polythene.

It was stored in refrigerator for further analysis.

Extraction Of Polyphenol From Pomegranate

Sample Preparation

pomegranate peel powder was taken in conical flask sample to solvent ratio was 1:10. ethanol was used as a solvent. Conical flask was placed in TECATOR Shaking Water Bath for

maceration. After maceration it was filtered through whatman filter paper no. 41. Filtrate was taken at BUCHI ROTAVAPOR to evaporate the solvent. Then it was taken in dry Amber glass bottle and stored for determination

of Total Polyphenols.

The proximate analysis of pomegranate peels powder results were shown in below the table. The moisture (%) content was 04+ 0.22, the ash content (%) was 05 +0.14 fat (%) content was 9.4 ± 0.1, similarly the pH, was found 3.75 ± 0.2, . Crude fiber was calculated (21 ± 0.6).

S#

Proximate analysis

Results

1. Moisture 04 ± 0.22(%)

2. Ash 05 ± 0.14(%)

3. Fat 9.4 ± 0.1(%)

4. pH 3.75 ± 0.2

5. Crude Fiber 21 ± 0.6(%)

6. Protein 8.719± 0.10(%)

7. Nitrogen 1.395± 0.30(%)

RESULTS AND DISCUSSION

Total polyphenols Content of Pomegranate Peels

In replication I we obtain 54 and in rep II and III we have gotten 56, 57 respectively mean of these three rep calculated was 55.67 with standard deviation of + 2.12

Sample Total polyphenol Content(mg/g gallic acid equivalent)

Rep I 54

Rep II 56

Rep III 57

Mean 55.67+ 2.12

Total polyphenol in pomegranate

Instruments Used for Extraction Phenolic Compound

Rotavapor

Instruments Used for Extraction Phenolic Compound

Glassware

Instruments Used for Extraction Phenolic Compound

Weighing Balance:

Instruments Used for Extraction Phenolic Compound

Water Shaking Bath

Instruments Used for Extraction Phenolic Compound

Hot air Oven

UV- VIS Spectroscopy

To measure the amount of Polyphenol compounds in the sample.

Muffle Furnace

Muffle furnace is used for ashing of product or sample.