International Workshop on diatoms, 16-20th of June 2008

Ecology & taxonomy of benthic diatoms in oligotrophic freshwater habitats

Limnological Station of the Trentino Nature & Science Museum at Lake Tovel

Exsiccata Carpato-pannonica

Collectio Diatomologica

MIRES

Dedicated to Akihiro Tuji and Marco Cantonati

Citation: Buczkó, K., Wojtal, A.Z. & Pócs, T. (2008): Exsiccata Carpato-pannonica, Collectio Diatomologica. Mires. Fasc. I. nos 1-9. pp. Hungarian Natural History Museum, Budapest pp. 21. http://www.buczko.eu/MiresExsiccata.pdf Published in 30 issue, primarily for the participants of the Workshop on “Ecology & taxonomy of benthic diatoms in oligotrophic freshwater habitats”

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Diatom collections date back to the earliest works in the filed of diatomology. To prepare slides and deposit them in collections are the first steps to explore the richness of taxa and increase our knowledge on diatoms in general. In the early age of diatomology, the most prominent experts had contacts and sent their own materials to each other. Thanks to this practice, many rare, interesting taxa have become available for later study in the large collections. (http://www.isdr.org/index.php/links/, http://home.planet.nl/~wolf0334/). Recently such kind of exchanging seems less intensive; the diatom collection of the Hungarian Natural History Museum received only a few slides after the death of our famous diatomist, József Pantocsek. We hit upon the idea of a Japanese scientist, Akihiri Tuji, who presented his data using an exiccata (Tuji 2007). He submitted the first part of the Japanise exsiccata on 18th May 2007 with 20 slides, and posted it as a present to our collection. When his material arrived at the Botanical Department, we were in the process of preparing a presentation for the Workshop “Ecology & taxonomy of benthic diatoms in oligotrophic freshwater habitats”, following on the invitation of Marco Cantonati. To present the most characteristic diatom taxa of mires in an Exsiccata seemed a perfect way of demonstration. This exsiccata set shows a selection of mire diatom species from the Apusenii Mountains, Romania. All of the raw materials are available in the Collectio of Algarum of BP. Methods The material was treated with hot hydrogen peroxide, nitric acid and the cleaned material was mounted in Zrax (refractive index is 1.7). For light microscope analysis, a LEICA DM LB2 was used (100 X HCX PLAN APO, and Fujifilm Digital Camera FinePix S2 Pro). Scanning electron microscopy was performed with a Hitachi S-2600N. AlgaTerra Information System was used for names of taxa and checks their validity (Jahn & Kusber 2008). We would like expressing our thanks to Horst Lange-Bertalot for some suggestions in the identification. References: Jahn, R. & Kusber, W.-H. (eds): AlgaTerra Information System [online]. Botanic Garden and Botanical Museum, Berlin-Dahlem, Freie Universität Berlin. 05 June 2008 Available from <http://www.algaterra.org>. Tuji, A. (2007) Algae Aquae Dulcis Japonicae Exsiccatae. Fasc. I. nos. 1-20. 29 pp. National Museum of Nature and Science Tsukuba

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Mires Exsiccata 1. Transition bog Hollow with filamentous algae

GPS: N46 31 39.5 E22 50 46.9 1300 m alt. Romanian Western Carpathians, Bihor County, Bihor Mountains, 1.2 km W of Sfoartea, NNW of Scarisoara, 1300 m. Transition bog, Carici stellulatae-Sphagnetum, Drosera rotundifolia BP: 2005/70 date: 27.07.2005. leg: Pócs, T. Sass-Gyarmati A. Brachysira spp. Figs 1-12. mainly Brachysira brebissonii R.Ross (2-5, 9) Eunotia compacta Hust. Figs 13-15. in A.W.F.Schmidt, Atlas Diatom.-Kunde, pl. 289: fig. 19 - 20. 1913. Frustulia erifuga Lange-Bert. & Krammer Fig. 16. in Iconogr. Diatomol. 2: p. 58. 1996. (note the convergent striae) Frustulia saxonica Rabenh. Figs 17-21 Süssw.-Diat. 61, p. 50, pl. 7: fig. 1. 1853. Pinnularia rupestris Hantzsch Figs 22-24. in Rabenh., Alg. Eur, no. 1203, fig. 118a: 1-12. 1861.

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Mires Exsiccata 2. Raised bog In an open black water window

GPS: N46 41 30.1 E23 01 06.9 1104 m alt. Romanian Western Carpathians, Cluj County, Gilăului Mountains. Raised bog at the NW corner of Beliş village along the tributary Dămbul Negru of Someşul Cald river. In an open black water window (Caricetum limosae). BP: 2008/153 pH = 3,78 Conductivity =74 µS Temperature = 16,5 C Date: 2007.08.19. Coll: Buczkó, K., Pócs, T.

Pinnularia rupestris Hantzsch Figs 1-4. in Rabenh., Alg. Eur, no. 1203, fig. 118: 1-12. 1861. Eunotia paludosa Grunow Figs 5-11. Eunotia (exigua) var. paludosa Grunow in Van Heurck 1881

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Mires Exsiccata 3. Floating bog

GPS: N46 35 46.8 E22 43 33.2 1278 m alt. Romanian Western Carpathians, Bihor County, Bihor Mountains, Padiş Plateau, 500 m W from the refuge hut Cabana Padiş. Floating bog with scattered Eriophorum vaginatum, BP: 2008/163 pH = 4,18 Conductivity = 94 µS Temperature = 21,8 Date: 2007.08.20 Coll: Buczkó, K., Pócs, T.

Pinnularia rupestris Hantzsch Figs 1-7. in Rabenh., Alg. Eur, no. 1203, fig. 118: 1-12. 1861. Eunotia paludosa Grunow Figs 8-12. Eunotia (exigua) var. paludosa Grunow in Van Heurck 1881 Kobayasiella micropunctata (H.Germ.) Lange-Bert. Figs 13-14. in Iconogr. Diatomol. 6: p. 267. 1999. (Navicula subtilissima var. micropunctata H.Germ.)

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Mires Exsiccata 4. Transition bog Peat mud in a small transition bog

N46 35 47.5 E22 42 29.7 1241 m alt

Romanian Western Carpathians, Bihor County, Bihor Mountains, Padiş Plateau, 2 km W from the refuge hut Cabana Padiş. (Caricie stellulatae-Sphagnetum) surrounded by spruce forest. BP: 2008/160b pH = 4.92 Conductivity =0 µS Temperature = 20 C Date: 2007.08.20. Coll: Buczkó, K., Pócs, T.

Eunotia compacta Hust. Figs 1-7. 19. in A.W.F.Schmidt, Atlas Diatom.-Kunde, pl. 289: fig. 19 - 20. 1913. Kobayasiella micropunctata (H.Germ.) Lange-Bert. Figs 8-12. in Iconogr. Diatomol. 6: p. 267. 1999. (Navicula subtilissima var. micropunctata H.Germ.) Pinnularia microstauron var. nonfasciata Krammer Figs 13-15. in Diatoms of Europe 1: p. 217, pl. 52: fig. 1-13, pl. 55: fig. 1-2. 2000. Pinnularia subcapitata W.Greg. Figs 16-18. in Quart. J. Microscop. Sci. 4: p. 9, pl 1: fig. 30. 1856.

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Mires Exsiccata 5. Raised bog

GPS N46 35 46.7 E22 43 34.0 1280 m alt

Romanian Western Carpathians, Bihor County, Bihor Mountains, Padiş Plateau, 500 m W from the refuge hut Cabana Padiş. At the centre of a raised bog (Eriophoro vaginato-Sphagnetum) with Vaccinium oxycoccos var. minor and with dwarfened spruce trees on clumps, at 1280 m alt. BP 2008/162 pH = 3.98 Conductivity =23 µS Temperature = 18.3 C Date: 2007.08.20. Coll: Buczkó, K., Pócs, T.

Pinnularia subcapitata W.Greg. Figs 1-3. in Quart. J. Microscop. Sci. 4: p. 9, pl 1: fig. 30. 1856. Pinnularia rupestris Hantzsch Figs 4-8. in Rabenh., Alg. Eur, no. 1203, fig. 118: 1-12. 1861. Eunotia compacta Hust. Figs 9-10. in A.W.F.Schmidt, Atlas Diatom.-Kunde, pl. 289: fig. 19 - 20. 1913. Eunotia paludosa Grunow Figs 11-17. Eunotia (exigua) var. paludosa Grunow in Van Heurck 1881

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Mires Exsiccata 6. Spring bog on slope

GPS: N46 35 49.5 E22 43 29.6 1286 m alt. Romanian Western Carpathians, Bihor County, Bihor Mountains, Padiş Plateau, 500 m W from the refuge hut Cabana Padiş. Spring bog on slope (Carici flavae-Eriophoretum) with Parnassia palustris and Pedicularis limnogenat. BP 2008/164 pH = 6.09 Conductivity =35 µS Temperature = 20.6 C Date: 2007.08.21. Coll: Buczkó, K., Pócs, T. Eunotia boreoalpina Lange-Bert. & Nörpel-Schempp Figs 1-6. in Iconogr. Diatomol. 5: p. 52. 1998. Eunotia bilunaris (Ehrenb.) Schaarschm. Figs 7-8. in Kanitz, Pl. Roman, p. 159. 1880. Tabellaria flocculosa (Roth) Kütz. Fig. 9. Kieselschal. Bacill., p. 127. 1844. Caloneis pulchra Messik. Figs 10-11. Krammer, K & H. Lange-Bertalot: Süsswasserflora 1985 Caloneis tenuis (W.Greg.) Krammer Fig. 12. in Biblioth. Diatomol. 9: p. 17. 1985. Placoneis ignorata (Schimanski) Lange-Bertalot Figs 13-15. in Iconogr. Diatomol. 9: p. 207. 2000. Syn: Navicula ignorata Schim. Syn: Navicula elginensis var. cuneata (M. Moller ex Foged) Lange-Bertalot Nitzschia sp. (N. cf. perminuta) Figs 16-17. Gomphonema acidoclinatum Lange-Bert. & E.Reichardt Figs 18-20 in Iconogr. Diatomol. 13: p. 160, pl. 92: fig. 1-19, pl. 93: fig. 1-3. 2004. Encyonema minutum (Hilse) D.G.Mann Fig. 21. in Round & al., Diatoms, p. 667. 1990. Cymbella gracilis (Ehrenb.) Kütz. Fig. 22. Encyonema silesiacum (Bleisch) D.G.Mann Fig. 23-24. in Round & al., Diatoms, p. 667. 1990.

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Mires Exsiccata 7. Small floating mire at the bottom of a carstic dolina

GPS: N46 36 37.7 E22 42 18.2 1255 m alt. Romanian Western Carpathians, Bihor County, Bihor Mountains, Padis Plateau, 400 m NNW from the refuge hut Cabana Varasoaia. BP: 2008/169 pH = 4.9 Conductivity =0 µS Temperature = 18.3 C Date: 2007.08.21. Coll: Buczkó, K., Pócs, T.

Pinnularia microstauron (Ehrenb.) Cleve Figs 1-4. in Acta Soc. Fauna Fl. Fenn. 8(2): p. 28. 1891. Eunotia rhomboidea Hust. Figs 5-11. in Arch. Hydrobiol. 43: p. 435, pl. 34: fig. 28; pl. 36: fig. 34-41. 1950.

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Mires Exsiccata 8. Small transition bog

GPS: N46 35 47.9 E22 42 29.2 1241 m alt. Romanian Western Carpathians, Bihor County, Bihor Mountains, Padiş Plateau, 2 km W from the refuge hut Cabana Padiş. Small transition bog (Caricie stellulatae-Sphagnetum) surrounded by spruce forest. On brown moss Caliergon sp. BP 2008/160 pH = 4.10 Conductivity =9 µS Temperature = 21.1 C Date: 2007.08.20. Coll: Buczkó, K., Pócs, T.

Eunotia cf. kruegeri Lange-Bert. Figs 1-3. in Iconogr. Diatomol. 13: p. 152, pl. 5: fig. 6-12, pl. 6: fig. 15-20. 2004. (this population is wider than the description) Eunotia subarcuatoides Alles, Nörpel & Lange-Bert. Figs 4-7. in Nova Hedwigia 53: p. 188, pl. 4: fig. 1-36. 1991. Pinnularia subcapitata W.Greg. Figs 8-9. in Quart. J. Microscop. Sci. 4: p. 9, pl 1: fig. 30. 1856. Pinnularia microstauron (Ehrenb.) Cleve Fig. 10. in Acta Soc. Fauna Fl. Fenn. 8(2): p. 28. 1891. Kobayasiella micropunctata (H.Germ.) Lange-Bert. Figs 11-17. in Iconogr. Diatomol. 6: p. 267. 1999. (Navicula subtilissima var. micropunctata H.Germ.)

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Mires Exsiccata 9. Open water in lagg zone

GPS: N46 35 54.5 E22 42 57.9 1249 m alt. Romanian Western Carpathians, Bihor County, Bihor Mountains, Padiş Plateau, 1.5 km W from the refuge hut Cabana Padiş, 650 m from the Vărăşoaia road junction. Open water zone (lagg) between transition and floating bog. On Drepanocladus sp. BP: 2008/171 pH = 4.6 Conductivity =8 µS Temperature = 23.9 C Date: 2007.08.21. Coll: Buczkó, K., Pócs, T.

Chrysophyceae statospores Figs 1-4. Pinnularia subcapitata W.Greg. Figs 5-6. in Quart. J. Microscop. Sci. 4: p. 9, pl 1: fig. 30. 1856. Eunotia valida Hust. Figs 7-9. in Pascher, Süsswasserflora 10, p. 178, fig. 229. 1930. Eunotia cf. exigua (Bréb. ex Kütz.) Rabenh. Fig. 10.

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