39

138 139

(goblet cell type) and non ciliated cell type showed

Leucocyte elastase in lung adenocarcinoma tissue.

Y. Ohshika, Y. Ozeki’ , K.Kase, K. Takagi, K. Kikuchi, S. Tanaka, G. Yamiya’ , T. Ogata

high oositive rate.

Department of Thoracic Surgery, ‘Biochemistry, National Defense Medical College, Tokorozawa, Saitama 359, Japan.

Human lung adenocarcinoma tissues were stained immunohistochemically with a polyclonal antibody against human leucocyte elastase(LE).

Twenty one of 97 cases(21.6%) showed an intense and granular staining in the basal part of the cancer cells.

The well differentiated papillary type showed a positive staining of LE more frequently than the othertypes of adenocarcinoma.

In the subclassification according to Shimosato’s criteria, the bronchial surface cell type with mucus

Immunohistochemical study of ADF/hTx expression in associated lymphoid tissue of lung cancer

Toshio Go, Hiromi Wada, Hiroshi Masutani’, Junji Yodoi*, Shigeki Hitomi; The Department of Thracic Surgery, Chest Disease Research Institute, Kyoto University; The Department of Biological Responses, Institute for Virus Research, Kyoto University*

A multifunctional peptide called Adult T cell leukemia Derived Factor (ADF) was originally isolated from human T Lymphotropic Virus-l transformed cells. Nowadays, the ADF cDNA has been cloned and the rADF has been shown to have co-cytokine activity and to have the synergism with IL1 or IL2. On the other hand, homology analysis revealed an structural relationship between ADF and thioredoxin, which had been reported as a reducing agent, coenzyme or radical scavenger.

kDFHP& &ere analogous to dendritic cells, and ihe po@lation

Sixty-three lung cancers and associated lymphoid tissues were investigated to demonstrate the expression of ADF. It was

density of ADFHPCs in early stage lung cancer was more than

found that most of the peripheral lymphoid tissues and draining lymphnodes of lung cancer contain ADF high productive cells (ADFHPCsj. The distribution oattern and uhenotvoe of

Theie was no significant difference in prognosis between LE positive and negative cases.

These results suggest that LE relates to differentiation or original cell type of cancer cell. But it is unclear that LE is concerned with proteolysis related to invasion or metastasis.

that in progressive stage lung cancer. s-100 protein, lysozme and CD68 were used to differentiate the accessory cells and to clarify that the most of ADFHPCs were dendritic cells. Expression of ADF may be a dendritic cell property that facilitates their accessory cell role within the associated lymphoid tissues of lung cancer.

140 141

ExpreSSion of HLA-DR antigen in early Expression of HLA-DR mtiaen h lung squamous cell luna cancers. cancers and Its correlation to

histologic types @ Jymphocutic T. Yazawa. H. Kamma and T. Ogata. Snflltration. Dept. of Pathology. Unl versi ty of Tsukuba, T. Ogata. T. Yazawa and H. Kamma. Dept. of Tsukuba. Japan.

HLA-DR antigen (HLA-DR) plays an

carcinoma lesions, while no or llttle

important role In presenting the various antigens to immune competent ccl 1s. To

expresslon was observed in the lesions of

clarify when the immunologlcal reacti on occurs In early squamous ccl 1 cancers, we studied the HLA-DR expression and the destructlon of basement membrane In 164 lesions of squamous metaplasia. non-invasive and early 1 nvaslve squamous ccl 1 carcinoma. using the Immunohlstochemical stalnlng with anti-HLA-DR and anti-laminin antlbodies. HLA-DR was expressed in 34% of early invasive

Path01 ogy . University of Tsukuba. Tsukuba, Japan.

expressed in cancer cells of 69% of 52

HLA-DR antigen CHLA-DR) plays an

adenocarcinomas.

important role in presenting the various antigens to immune competent ccl 1s. An

24% of 21 large ccl 1

immunohistochemical study was performed on 125 lung cancers of various histologic types to clarify the correlations between expression of HLA-DR and histologic types of cancers or stromal lymphocytlc reaction. These specimens examined here were selected from the tumors smaller than 3 cm In diameter to avoid the influence of the reaction against the tumor necrosis. HLA-DR was

squamous metaplasia and non-Invasive squamous ccl 1 carcinoma. Its expression was closely related to the degree of destruction of basement membrane and was associated with lymphocytlc 1 nf i 1 trates surrounding the lesions. These flndlngs might suggest that the immunological react1 on agal nst bronchogenic carcl nomas . 1 f the tumor-related antlgens are present, occurs after the basement membranes are destroyed.

carcinomas, and 21% of 32 squamous ccl 1 carci nomas. No expression was found in al 1 of 20 small ccl 1 carcinomas. Furthermore, HLA-DR was expressed in the cases with lymphocytlc infiltratlon in the surrounding stroma of cancers and its expresslon was closely correlated to the degree of lymphocytic infiltration. This study reveals that HLA-DR expression varies among the hlstologlc types of lung cancers. It might suggest that Immunological reaction agalnst lung cancers is related to HLA-DR expression.