experiment 4- gene expression study in arabidopsis thaliana
DESCRIPTION
Experiment 4- Gene Expression Study in Arabidopsis Thaliana. RNA Blot. Quantitative RT-PCR. Tools For Studying Differential Gene Activity. Microarrays. What Are The Major Differences Between DNA and RNA?. RNA Has a Uracil Instead of Thymine. RNA Has Ribose Sugar in Nucleotide. - PowerPoint PPT PresentationTRANSCRIPT
Experiment 4- Gene Expression Study in Arabidopsis Thaliana
Control
SRYTarget
RNA
RNA Blot
Seed mRNA Flower mRNA
Primers SpecificFor bobg mRNA
Quantitative RT-PCR
Microarrays
Tools For Studying Differential Gene Activity
What Are The Major Differences Between DNA and RNA?
RNA Has a Uracil Instead of Thymine
RNA Has Ribose Sugar in Nucleotide
Eukaryotic RNAs Are Single-Stranded Polynucleotides
NucleotidesJoined By
Phosphodiester Bonds Like All Nucleic Acids
Order 5’ 3’ Leads to Function:
Co-Linear With Gene Sequence
RNA Has Intra-Strand Double Helices or Secondary Structure
Total RNA Isolation Workflow
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Collecting plant
materials (1-6)
Lysis (7-11)
(12-16)
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Washing (21-32)
Precipitation & Binding
(17-20)
Elute RNA (33-
40)
Determining Concentratio
n
1. RNA Isolation
2. Remove Genomic DNA
RNase-Free DNase I
3. ElectrophoresisDetermining Quality
of Isolated RNA
Determining Quality of Isolated Total RNA Using A Capillary Gel Electrophoresis System
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Determining Quality of Isolated Total RNA Using A Capillary Gel Electrophoresis System
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Determining Quality of Isolated Total RNA Using A Capillary Gel Electrophoresis System-
Part II
Leaf
Leaf
Silique
Silique