exiprep magnetic bead type manual nucleic acid ......dna/rna extraction kit can automatically...

Automatic Nucleic Acid Extraction: ExiPrep™ Magnetic Bead Type Manual Nucleic Acid Extraction: MagListo™Spin Column Type Manual Nucleic Acid Extraction: AccuPrep®96-Well Vacuum Block Type Manual Nucleic Acid ExtractionSolution Type Manual Nucleic Acid Extraction: AccuZol™Accessory

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DNA/RNA Purification ····························································································································3

Genomic DNA Extraction Kit Blood Genomic DNA Kit ················································································································································4

Tissue Genomic DNA Kit ················································································································································6

Bacteria Genomic DNA Kit ············································································································································8

Plant Genomic DNA Kit ··············································································································································· 10

Total RNA Extraction Kit Tissue Total RNA Kit ······················································································································································ 12

Plant Total RNA Kit························································································································································· 14

Viral DNA/RNA Extraction Kit Viral DNA/ RNA Kit························································································································································· 16

DNA/RNA Preparation Instrument ExiPrep™ 16 Plus → Go to M. Instrument & Devices

Automatic Nucleic Acid Extraction: ExiPrep™

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DNA/RNA Purification

The extraction and purification of nucleic acid is very important step in molecular biology, biotechnology, biochemistry, laboratory medicine, etc, because the result of experiments, such as PCR/RT-PCR, Real-Time PCR/RT-qPCR, sequencing, subcloning, gene cloning, molecular diagnostics, etc, depends on the quality of an extracted and purified nucleic acid. In addition, it is important to select the extraction kit optimized for samples in order to extract DNA/RNA effectively from blood, animal tissue, culture cell, plant tissue as well as biological specimen. Bioneer has supplied optimized kits for nucleic acid extraction and purification to help various experiments of customers. Bioneer’s nucleic acid extraction and purification kit will be with you in your successful research.

Bioneer has 5 types of nucleic acid extraction product lines from manual kits to automated platforms.

ExiPrep™ DNA/RNA Extraction Kit can automatically extract high quality nucleic acids from different 16 samples, such as blood, animal tissue, cell cultures and plat tissues, with ExiPrep™ 16 series instruments (ExiPrep™ 16 Plus, ExiPrep™ 16 Dx). This product uses a ‘Pre-Loaded Buffer Cartridge System’ which provides user convenience and reproducibility by loading all necessary reagents within the kit. Pre-installed protocols are optimized for each specific sample and target nucleic acid type to provide robust results run after run. The silica magnetic particles used in the product have high DNA/RNA binding capacity and were developed using in-house nanoparticle R&D expertise.

MagListo™ Magnetic Nanobead-based Extraction Kit is a product leading change and innovation in nucleic acid extraction market. The product of single spin column type occupying most of market share has limited at initial volume of sample because of some problems such as a binding capacity of membrane filter, clogging, etc. In case of large scale, shortcoming is that an extraction step takes a lot of time due to centrifugation step. Because it is able to remove bindingmaterials (nanobead) by magnetism, nucleic acid extraction using magnetic nanobead can easily separate cell lysate and nucleic acid in short time. In addition you can use a kind of this kit in various scales by just adjusting of volume of magnetic nanobead and buffer according to sample amount.

AccuPrep® DNA/RNA Extraction Kit is Bioneer’s the most popular nucleic acid extraction product type which uses a single spin column with glass micro fiber filters. Bioneer provides various products for high-purity and high-yield Genomic DNA, Plasmid DNA, Viral RNA or fragment DNA. The AccuPrep® Nano-Plus Plasmid DNA Extraction Kit uses Bioneer’s proprietary cell fragment and protein removal technology to reduce plasmid DNA prep time from the usual 30 minutes to a revolutionary 10 minutes. The nano-particles caught only target DNA/RNA from a complex among cellular debris, denatured proteins and other unnecessary materials, in a clear lysate within just 1 minute.

AccuPrep® DNA Extraction Kit for 96 well vacuum manifold is designed for high-throughput DNA extraction. The 96-well plate format kit is attached to a vacuum manifold (BioVac™ 96 vacuum manifold) to extract and purify DNA from up to 96 samples simultaneously.

The final product group is the AccuZol™ products which use the traditional organic solvent RNA extraction methods. These products contain phenol to stop the activity of various nucleases and extract high-quality nucleic acids. These products are especially suitable for large input volumes or a manual workflow.

Overview

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■ DescriptionBlood Genomic DNA Kit enables extraction of genomic DNA from whole blood samples with high yield and purity using automated nucleic acid extraction instrument, ExiPrep™ 16 series (ExiPrep™ 16 Plus, ExiPrep™ 16 Dx) and ExiProgen™.

■ Features and Benefits● All reagents are pre-dispensed into a pre-filled buffer cartridge

system

● The kit contains all required consumables such as disposable filter tips, elution tubes, and reaction tubes

● The kit is able to process up to 16 samples in a single run

● Bioneer’s patented magnetic silica beads ensure high yield and purity

● The automated process ensures reproducible results

■ Specifications

Starting volume 200 μl

Elution volume 50 - 200 μl

Expected yield 3 - 5 μg

Expected purity (A260/280) > 1.8

■ ApplicationPCR, Real-Time PCR, Cloning, SNP analysis, Pharmacogenomics, and Genetics

■ Experimental Data

Figure 1. Agarose gel electrophoresis results of genomic DNA extracted from whole blood.Agarose gel electrophoresis results of 100 ng genomic DNA extracted from 200 μl whole blood sample.

M: Size marker (1 kb DNA Ladder, D-1040, Bioneer) Odd number lane: Extraction with blood sample Even number lane: Extraction with D.W. only

Figure 2. Real-Time PCR results of genomic DNA extracted from whole blood.Real-Time PCR results using five primer/probe sets of targets related to Alzheimer’s Disease and run on Exicycler™ 96 Real-Time PCR System (Cat. no. A-2060) from genomic DNA extracted from 200 μl whole blood samples.

Blood Genomic DNA Kit

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■ Procedure

(200 µl)

■ Ordering Information

Cat. no. Product Description Instrument Type

K-3215 ExiPrepTM Blood Genomic DNA Kit, 96 rxn ExiPrepTM 16

K-4211 ExiPrepTM Plus Blood Genomic DNA Kit, 96 rxn ExiPrepTM 16 Plus, ExiProgen™

K-4411 ExiPrepTM Dx Blood Genomic DNA Kit, 96 rxn ExiPrepTM 16 Dx

Blood Genomic DNA Kit

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Tissue Genomic DNA Kit

■ DescriptionTissue Genomic DNA Kit enables extraction of genomic DNA from animal tissue samples with high yield and purity using automated nucleic acid extraction instrument, ExiPrep™ 16 series (ExiPrep™ 16, ExiPrep™ 16 Plus) and ExiProgen™. The Tissue Lysis Buffer with Proteinase K included in the kit is optimized for various animal tissues, which increase extraction yield of genomic DNA.

■ Features and Benefits● All reagents are pre-dispensed into a pre-filled buffer cartridge

system



● Bioneer’s patented magnetic silica beads ensure high yield and purity.


■ Specifications

Starting amount Up to 25 mg




■ ApplicationPCR, Real-Time PCR, Gene cloning, SNP analysis, and Genetics


Figure 1. Electrophoresis of genomic DNA extracted from animal tissue.Genomic DNA was extracted from 25 mg of animal tissue (lung), then followed by 1% agarose gel electrophoresis. M: Size marker (1 kb DNA Ladder, D-1040, Bioneer) S lane: Extraction with tissue sample B lane: Extraction with D.W. only

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Tissue Genomic DNA Kit

■ Procedure


Cat. no. Product Description Instrument type

K-3225 ExiPrepTM Tissue Genomic DNA Kit, 96 rxn ExiPrepTM 16

K-4212 ExiPrepTM Plus Tissue Genomic DNA Kit, 96 rxn ExiPrepTM 16 Plus, ExiProgen™

K-7030 Tissue Homogenization Set, 100 rxn

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Bacteria Genomic DNA Kit

■ DescriptionBacteria Genomic DNA Kit enables extraction of genomic DNA from Gram (-) and Gram (+) bacteria with high yield and purity using automated nucleic acid extraction instrument, ExiPrep™ 16 series (ExiPrep™ 16, ExiPrep™ 16S, ExiPrep™ 16 Plus, and ExiPrep™ 16 Dx) and ExiProgen™.

■ Features and Benefits

● All reagents in the kit are pre-dispensed into a pre-filled buffer cartridge system

● The kit provides a highly optimized buffer for lysis and resuspension of bacterial cells

● The kit contains all required consumables such as a disposable filter tip, an elution tube, etc




● This Bacteria Genomic DNA Kit is also applied to some type of animal cell lines

■ Specifications

Starting amount ~ 1x109 cells


Expected yield 10 mg


■ ApplicationPCR, Real-Time PCR, Gene cloning, Genetics, and Classification of bacteria


Figure 1. Genomic DNA purification from E. coli cell (1x109 cells).Genomic DNA was extracted from 1x109 of E. coli cells followed by agarose gel electrophoresis.by 1% agarose gel electrophoresis. M: Size marker (1 kb DNA Ladder, D-1040, Bioneer) S lane: Extraction with tissue sample B lane: Extraction with D.W. only

Figure 2. Real-Time PCR results (MTB & NTM) of genomic DNA extracted from sputum samples.

Real-Time PCR results using an AccuPower® MTB & NTM EX Real-Time PCR Kit (MTN-1111, Bioneer) and run on Exicycler™ 96 Real-Time Quantitative Thermal Block (A-2060, Bioneer) from genomic DNA extracted from 10 ml of sputum samples.

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Bacteria Genomic DNA Kit

■ Procedure



K-3245 ExiPrepTM Bacteria Genomic DNA Kit, 96 rxn ExiPrepTM 16, ExiPrepTM 16S

K-4214 ExiPrepTM Plus Bacteria Genomic DNA Kit, 96 rxn ExiPrepTM 16 Plus, ExiProgenTM

K-4414 ExiPrepTM Dx Bacteria Genomic DNA Kit, 96 rxn ExiPrepTM 16 Dx

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Plant Genomic DNA Kit

■ DescriptionPlant Genomic DNA Kit enables extraction of genomic DNA from plant samples, such as leaf tissues, seeds and roots, etc, with high yield and purity using automated nucleic acid extraction instrument, ExiPrep™ 16 series (ExiPrep™ 16, ExiPrep™ 16 Plus) and ExiProgen™. Plant tissues can be efficiently digested and disrupted using the included Plant Lysis Buffer and Protease.




● The kit provides a highly optimized Plant Lysis Buffer. (for leaf tissue sample: Plant Lysis Buffer ①, for seed sample: Plant Lysis Buffer ②)




■ Specifications

Starting amount 100 mg




■ ApplicationPCR, Real-Time PCR, Gene cloning, Genetics, and GMO detection


Figure 1. Electrophoresis of genomic DNA extracted from chinese cabbage leaf tissue (100 mg)

Genomic DNA was extracted followed by agarose gel electrophoresis.M: Size marker (1 kb DNA Ladder, D-1040, Bioneer),Lane 1~8: Extracted genomic DNA

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Plant Genomic DNA Kit

■ Procedure



K-3255 ExiPrepTM Plant Genomic DNA Kit, 96 rxn ExiPrepTM 16

K-4215 ExiPrepTM Plus Plant Genomic DNA Kit, 96 rxn ExiPrep™ 16 Plus, ExiProgen™

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Tissue Total RNA Kit

■ DescriptionTissue Total RNA Kit enables extraction of total RNA from animal tissue with high yield and purity using automated nucleic acid extraction instrument, ExiPrep™ 16.




● The kit provides a highly optimized lysis buffer for animal tissue samples



● Automated process ensures reproducible results

■ Specifications

Liver Eye-ball Brain Tail-tip

Sample amount ~ 15 mg ~ 1 ea ~ 20 mg ~ 15 mg

Elution volume 100 μl 100 μl 100 μl 100 μl

Expected yield (µg) ~ 25 μg ~ 10 μg ~ 8.5 μg ~ 4 μg

Purity (A260/280) > 1.9 > 1.9 > 1.9 > 1.9

■ ApplicationRT-PCR, RT-qPCR, cDNA synthesis, and Chip-array analysis


Figure 1. Total RNA analysis purified from rat tissue.

Total RNA was extracted from 15 mg of rat liver tissue followed by RNA quality analysis using LabChip GX (Caliper Life science).

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Tissue Total RNA Kit

■ Procedure



K-3325 ExiPrepTM Tissue Total RNA Kit, 96 rxn ExiPrepTM 16

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Plant Total RNA Kit

■ DescriptionPlant Total RNA Kit enables extraction of total RNA from plant samples with high yield and purity using automated nucleic acid extraction instrument, ExiPrep™ 16 Plus and ExiProgen™. Plant tissues can be efficiently digested and disrupted using the included Plant Lysis Buffer.



● The kit contains all required consumables such as disposable filter tips, elution tubes, and reaction tubes, etc

● The kit provides a highly optimized lysis buffer for plant tissue samples




■ Specifications

Leaf tissue (wet) Seed

Chinesecabbage Brocoli Chinese

cabbageRed

bean

Sample amount 100 mg 100 mg 100 mg 100 mg

Elution volume 50 μl 50 μl 50 μl 50 μl

Expected yield (µg) ~ 15 μg ~ 8 μg ~ 5 μg ~ 9 μg

Purity (A260/280) >2.0 >2.1 >1.8 >1.5

■ ApplicationRT-PCR, RT-qPCR, cDNA synthesis, and Chip-array analysis


Figure 1. Electrophoresis result with extracted plant total RNA.Results of gel electrophoresis of total RNA extracted from various plant samples. Expected yields were on average 5-15 μg depending on sample

types. Also expected purities were at least 2.0 (A260/A280) and 1.5 (A260/A230).

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Plant Total RNA Kit

■ Procedure



K-4244 ExiPrepTM Plus Plant Total RNA Kit, 96 rxn ExiPrepTM 16 Plus, ExiProgen™

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Viral DNA/RNA Kit

■ DescriptionViral DNA/RNA Kit enables extraction of viral DNA/RNA from various cell-free body fluids and swabs, such as serum, plasma, CSF, saliva, etc, with high yield and purity using automated nucleic acid extraction instrument, ExiPrep™ 16 series (ExiPrep™ 16, ExiPrep™ 16 Plus, and ExiPrep™ 16 Dx) and ExiProgen™. User’s manual included in the kit explains the optimized pre-treatment methods for each type of samples in detail. Buffer cartridge contains lyophilized carrier RNA to prevent RNA degradation and to promote efficient binding of RNA to magnetic silica beads, and lyophilized Protease K

to lysis sample efficiently.



● The kit contains all required consumables such as disposable filter tips, elution tubes, and reaction tubes etc




■ ApplicationPCR, RT-PCR, Real-Time PCR, and RT-qPCR


Figure 1. Real-Time PCR result (HBV) of viral DNA extracted from serum.

Real-Time PCR results using an AccuPower® HBV Quantitative PCR Kit (HBV-1111, Bioneer) and run on Exicycler™ 96 Real-Time Quantitative Thermal Block (A-2060,

Bioneer) with extracted viral DNA.

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Viral DNA/RNA Kit

■ Procedure



K-3535 ExiPrepTM Viral DNA/RNA Kit, 96 rxn ExiPrepTM 16

K-4271 ExiPrepTM Plus Viral DNA/RNA Kit, 96 rxn ExiPrepTM 16 Plus, ExiProgen™

K-4272 ExiPrepTM Plus Viral DNA Kit, 96 rxn ExiPrepTM 16 Plus, ExiProgen™

K-4273 ExiPrepTM Plus Viral RNA Kit, 96 rxn ExiPrepTM 16 Plus, ExiProgen™

K-4471 ExiPrepTM Dx Viral DNA/RNA Kit, 96 rxn ExiPrepTM 16 Dx

K-4472 ExiPrepTM Dx Viral DNA Kit, 96 rxn ExiPrepTM 16 Dx

K-4473 ExiPrepTM Dx Viral RNA Kit, 96 rxn ExiPrepTM 16 Dx

Total RNA Extraction Kit MagListo™ 5M Cell Total RNA Extraction Kit ······································································································· 19

MagListo™ 5M Tissue Total RNA Extraction Kit ·································································································· 21

Genomic DNA Extraction Kit MagListo™ 5M Genomic DNA Extraction Kit ······································································································ 23

MagListo™ 5M Plant Genomic DNA Extraction Kit ··························································································· 25

MagListo™ 5M Forensic Sample DNA Extraction Kit ························································································ 27

Plasmid DNA Extraction Kit MagListo™ 5M Plasmid Extraction Kit ················································································································· 28

Fragment DNA Purification Kit MagListo™ 5M Gel Extraction Kit ·························································································································· 30

MagListo™ 5M PCR Purification Kit ······················································································································· 31

Viral DNA/RNA Extraction Kit MagListo™ 5M Viral DNA/RNA Extraction KitNEW ····························································································· 32

Circulating Cell free DNA Extraction Kit MagListo™ 5M Circulating Cell free DNA Extraction KitNEW ········································································· 34

MagListo™ Magnetic Separation Rack ························································································ 36

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MagListo™ 5M Cell Total RNA Extraction Kit

■ DescriptionMagListo™ 5M Cell Total RNA Extraction Kit is designed for quick extraction of total RNA from various cell types using Magnetic Nanobeads. With MagListo™ Magnetic Separation Rack and 5M Cell Total RNA Extraction Kit, it takes only eight minutes to extract total RNAs from cell lysate in mini scale even without the use of centrifugation! The purity and yield of extracted RNA are comparable to those of any type of products in the market. This kit also provides greater convenience to customers by serving different scales of extraction - mini, midi if necessary - with a single reagent kit.

■ Features and Benefits● Extract total RNA from various cell types and even from low copy

cells

● Take only 8 minutes in mini scale and 13 minutes in midi scale

● A single kit serve mini and midi experiment

● No need of expensive instrument except MagListo™ Magnetic Separation Rack

■ Specifications

Mini scale Midi scale

Starting cell number 10 - 5x106 cells 5x106 - 2x107 cells

Preparation time ~ 8 min ~ 13 min

Elution volume 50 -100 μl 250 - 500 μl

Expected RNA yield Up to 100 μg Up to 400 μg

Expected purity A260/280 > 2.0

*RNA content can vary according to cell type.

■ Typical Yields of Total RNA

Cell type (1x106) Yield

HeLa 10 -15 μg

293T 20 -30 μg

Balbe3T3 20 -30 μg

Huh7 10 -15 μg

■ ApplicationRT-PCR and Real-Time RT-PCR, cDNA synthesis, Northern, dot and slot blot analysis, Primer extension, Microarrays, and RNAseq


Figure 1. Comparison of total RNA purified with MagListo™ 5M Cell Total RNA Extraction Kit and competitor’s kit (spin column type).

1x106 and 5x106 HeLa cell were used for the comparison. Total RNA purification was performed with the MagListo™ 5M Cell Total RNA Extraction Kit and the competitor’s product respectively. The bands of purified total RNA were illustrated by 1% denaturing agarose gel electrophoresis.

Figure 2. RT-qPCR comparison of RNA isolated from 10-106 HeLa cells purified with MagListo™ and competitor’s kit (spin column type).

The amplification plot for reactions containing cDNA prepared from an average of 10, 102, 103, 104, 105, and 106 Hela cells (triplicate samples) with the MagListo™ Cell Total RNA Extraction Kit (left). Comparison of Standard curves from qPCR results using cell total RNA purified with Bioneer MagListo™ Cell Total RNA Extraction Kit and competitor Q kit. The standard curve produced by plotting the log of the average initial HeLa cell number against the cycle threshold (CT) is linear.

Fast & easy RNA extraction from various cell types. A single kit serves mini and midi scale!

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■ Procedure


Cat. no. Product Description

K-3611 MagListoTM 5M Cell Total RNA Extraction Kit, 100 rxn in mini

MagListo™ 5M Cell Total RNA Extraction Kit

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MagListo™ 5M Tissue Total RNA Extraction Kit

■ DescriptionMagListo™ 5M Tissue Total RNA Extraction Kit is designed for quick extraction of total RNA from various tissue and cells using Magnetic Nanobeads. With MagListo™ Magnetic Separation Rack and 5M Tissue Total RNA Extraction Kit, it takes only 10 minutes to extract total RNAs from cell lysate in mini scale even without the use of centrifugation! The purity and yield of extracted RNA are comparable to those of any type of products in the market. This kit also provides greater convenience to customers by serving different scales of extraction - mini, midi if necessary - with a single reagent kit.

■ Features and Benefits● Extract total RNA from various cell types and even from low

copy cells

● Take only 10 minutes in mini scale and 15 minutes in midi scale

● A single kit serve mini and midi experiment

● No need of expensive instrument except MagListoTM Magnetic Separation Rack

■ Specifications

Mini scale Midi scale

Starting sample ≤30 mg tissue 3x106 cells

≤200 mg tissue 1x107 cells

Preparation time ~ 10 min ~ 15 min

Elution volume 50 μl 500 μl

Expected RNA yield Up to 100 μg Up to 500 μg


*RNA content can vary according to tissue type.

■ Typical Yields of Total RNA

Tissue type (10 mg) Yield

Kidney 20-30 μg

Liver 40-60 μg

Spleen 20-40 μg

Thymus 30-50 μg

Lung 10-20 μg

■ ApplicationRT-PCR and Real-Time RT-PCR, cDNA synthesis, Northern, dot and slot blot analysis, Primer extension, Microarrays, and RNAseq


Prep kit No. Yield (μg) A260/280 A260/230

MagListoTM

1 58.6 2.09 2.03

2 60.3 2.09 1.95

3 55.7 2.08 2.02

4 58.7 2.09 1.97

Competitor

5 36.8 2.04 1.76

6 38.3 2.05 1.78

7 34.5 2.03 1.91

8 37.9 2.05 1.48

Figure 1. Comparison of total RNA purified from liver tissue (10 mg) with MagListoTM 5M Tissue Total RNA Extraction Kit and competitor’s kit (spin column type)

Prep kit RNA Quality Score

rRNA Area Ratio[28S/18S]

rRNA Height Ratio

[28S/18S]

MagListoTM

10.0 3.81 1.90

10.0 3.79 1.90

10.0 3.36 1.73

10.0 3.51 1.80

Competitor

10.0 3.75 1.90

10.0 4.41 2.06

10.0 4.15 2.06

10.0 3.94 1.93

Table1. Result of capillary electrophoresis of total RNA extraction from Hela cell using LabChip.

Fast & easy RNA extraction from various tissue and cell type. A single kit serves mini and midi scale!

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■ Procedure



K-3613 MagListoTM 5M Tissue Total RNA Extraction Kit, 100 rxn in mini

MagListo™ 5M Tissue Total RNA Extraction Kit

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MagListo™ 5M Genomic DNA Extraction Kit

■ DescriptionMagListo™ 5M Genomic DNA Extraction Kit is designed for quick extraction of genomic DNAs from various sample types such as whole blood, cultured cell, animal tissues and bacterial cells using Magnetic Nanobeads. With MagListo™ Magnetic Separation Rack and the 5M Genomic DNA Extraction Kit, it takes only five minutes to extract genomic DNA from cell lysate in mini scale even without centrifugation! The purity and yield of extracted genomic DNA are comparable to those of any type of products in the market. This kit also provides greater convenience to customers by serving different scales of extraction- mini, midi and maxi - with a single reagent kit.

■ Features and Benefits● Extract genomic DNA from various samples such as blood,

cultured cell, animal tissue and bacteria (Gram (-), (+))

● Extract genomic DNA even from 104 cell samples

● Takes only 5 minutes in mini, 10 minutes in midi and 15 minutes in maxi scale (Hands-on time)

● A single kit serves all scales of experiments: mini, midi and maxi

● No need of expensive extra instrument (vacuum system or air pressure system etc) except MagListo™ Magnetic Separation Rack

■ ApplicationGene Cloning, PCR, Real-Time PCR, Southern Blotting, and SNP genotyping


Figure 1. Comparison of genomic DNA from human whole blood (200 μl) purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (spin column type).

M: Bioneer 1 kb ladder 1-4: Blood genomic DNA purified with Bioneer MagListoTM Yield; 6.45 μg, Purity(A260/280); 1.855-7: Blood genomic DNA purified with competitor Q kitYield; 5.53 μg, Purity(A260/280); 1.85

Figure 2. Comparison of Real-Time qPCR data of genomic DNA from HeLa cell (1x102 ~ 1x105) purified with MagListoTM 5M Genomic DNA Extraction Kit and competitor’s kit (spin column type).

Comparison of standard curves from qPCR results using genomics DNA purified with Bioneer MagListoTM 5M Genomic DNA Extraction Kit and competitor Q kit.

Fast & easy genomic DNA extraction from blood, animal tissue and cell culture! A single kit serves mini, midi and maxi scale!

■ Specifications

Sample Type Minis scale (Typical yield) Midi scale (Typical yield) Maxi scale (Typical yield)

Whole blood 200 μl (< 10 μg) 2 ml (< 80 μg) 4 ml (< 150 μg)

Cultured cell ~ 1x106 (< 12 μg) ~ 5 x 106 (< 60 μg) ~ 1x107 (< 120 μg)

Animal tissue ~ 25 mg (< 10 μg) ~ 100 mg (< 40 μg) ~ 250 mg (< 120 μg)

Bacteria (Gram(-), (+)) ~ 1x109 (< 15 μg) ~ 5x109 (< 80 μg) ~ 1x1010 (< 150 μg)

Preparation time ~ 5 min ~ 10 min ~ 15 min


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■ Procedure



K-3603 MagListoTM 5M Genomic DNA Extraction Kit, 100 rxn in mini

MagListo™ 5M Genomic DNA Extraction Kit

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MagListo™ 5M Plant Genomic DNA Extraction Kit

■ DescriptionMagListoTM 5M Plant Genomic DNA Extraction Kit is an innovative product able to rapidly extract from plant sample such as leaf, tissue, root, seed, etc using Magnetic Nanobead. With MagListoTM Magnetic Separation Rack and the 5M Plant Genomic DNA Extraction Kit, it takes only five minutes to extract genomic DNA from plant samples (leaf, tissue, root, seed, etc) in mini scale even without centrifugation! This kit also provides greater convenience to customers by serving different scales of extraction- mini, midi and maxi - with a single reagent kit.

■ Features and Benefits● Minimum hands-on time (mini-5 minutes, midi-10 minutes, & maxi-15 minutes)● The kit provides a highly optimized Plant Lysis Buffer for various

plant tissues and seeds ● No need of expensive extra instrument (vacuum system or air

pressure system etc) except MagListo™ Magnetic Separation Rack.

■ ApplicationGene Cloning, PCR, Real-Time PCR, Southern Blotting, and SNP genotyping


Figure 1. Comparison of genomic DNA from broccoli tissue (100 mg) purified with MagListoTM 5M Plant Genomic DNA Extraction Kit and competitor’s kit (spin column type).

M: Bioneer 1 kb ladder 1-2: Plant genomic DNA purified with Bioneer MagListoTM

Yield; 14.6 μg, Purity(A260/280); 1.813-4: Plant genomic DNA purified with competitor Q kit

Yield; 6.1 μg, Purity(A260/280); 1.80

Fast & easy genomic DNA extraction from plant tissue without centrifuge! A single kit serves mini, midi and maxi scale!

■ Specifications

Mini scale Midi scale Maxi scale

Plant tissue ~ 100 mg ~ 500 mg ~ 1 g

Seed ~50 mg ~ 250 mg ~ 500 mg

Elution volume 100 μl 500 μl 1 ml

Typical DNA yield 5~15 μg 15~80 μg 30~150 μg

Expected purity A260/280 > 1.7, A260/230 > 1.2

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■ Procedure



K-3605 MagListoTM 5M Plant Genomic DNA Extraction Kit, 100 rxn in mini

MagListo™ 5M Plant Genomic DNA Extraction Kit

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MagListo™ 5M Forensic Sample DNA Extraction Kit

■ DescriptionMagListo™ 5M Forensic Sample DNA Extraction Kit is designed for quick extraction of total DNA from various forensic samples, such as whole blood, saliva, urine, fingerprint, hair, finger nail, bone, and et cetra, using Magnetic Nanobeads. With MagListo™ Magnetic Separation Rack and 5M Forensic Sample DNA Extraction Kit, it takes only 10 minutes to extract total DNA from cell lysate in mini scale without the need of centrifugation. The extracted DNA using this kit is ready-to-use for further applications in its purity and yield.

■ Features and Benefits● Takes only 10 minutes in mini scale


■ Specifications

Feature Specification

Sample type Various kinds of forensic samples

Preparation time ~ 10 min


■ ApplicationPCR, Real-Time PCR, SNP genotyping, and STR analysis

■ Experimental Data(A) Saliva (10 μl) & Blood (10 μl) stains from clothes (7 mm diameter)

(B) Hair 1 strand (25 cm)

Figure 1. Comparison of Real-Time qPCR Ct values of DNA extracted using MagListo™ and competitor’s products (spin column type)

Figure 2. Comparison of Genomic DNA from Porcine Skeletal Muscle Tissue purified with MagListoTM Forensic Sample DNA Extraction Kit and competitor’s kit (spin column type)

M: 1 kb Ladder

1 mgMagListoTM- yield: 1.35 μg purity(A260/280): 1.95Competitor- yield: 0.57 μg purity(A260/280): 1.86

5 mgMagListoTM- yield: 5.22 μg purity(A260/280): 1.90Competitor- yield: 2.81 μg purity(A260/280): 1.88

Fast & easy genomic DNA extraction from forensic samples (Dried blood spot, hair, nail clipping, and saliva etc.)



K-3615 MagListoTM 5M Forensic Sample DNA Extraction Kit, 100 rxn in mini

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MagListo™ 5M Plasmid Extraction Kit

■ DescriptionMagListo™ 5M Plasmid Extraction Kit is an innovative product to extract plasmid DNA from bacterial cultures using Magnetic Nanobeads. With MagListo™ Magnetic Separation Rack and 5M Plasmid Extraction Kit, it takes only five minutes in mini scale even without centrifugation! This kit also provides greater convenience to customers by serving different scales of extraction- mini, midi and maxi - with a single reagent kit.

■ Features and Benefits● Magnetic bead technology enables rapid extraction: mini-5

min., midi-10 min., maxi-15 min● One kit serves mini, midi and maxi scale prep● No need of expensive extra instrument (vacuum system or air

pressure system etc) except MagListo™ Magnetic Separation

Rack

■ ApplicationGene cloning, PCR, Real-Time PCR, sequencing, transformation, transfection, and in vitro transcription/translation


Figure 1. Electrophoresis data of 500 ng of several size plasmids purified with MagListoTM 5M Plasmid Extraction Kit.

M1: Bioneer 100 bp ladder1: 3.5 kb plasmid DNA2: 5.4 kb plasmid DNA3: 8 kb plasmid DNAM2: Bioneer 1 kb ladder

Figure 2. Comparison of plasmid DNAs purified using MagListoTM and competitors’ products (magnetic bead type).

M1: Bioneer 100 bp ladder1-2: Plasmid DNA purified with Bioneer MagListoTM (3.5 kb / 5 kb)3-4: Plasmid DNA purified with competitor P kit (3.5 kb / 5 kb)5-6: Plasmid DNA purified with competitor I kit (3.5 kb / 5 kb)M2: Bioneer 1 kb ladder

Concentration(ng/ul)

63.9 2.01

2.09

1.93

1.97

2.14

2.1

86.1

45.7

37.2

38.6

40.1

1(B)

2(B)

3(B)

4(B)

5(B)

6(B)

A260/280

Just 5 minutes to extract nucleic acids without centrifuge! A single kit serves mini, midi and maxi scale!

■ Specifications

Mini scale Midi scale Maxi scale

Starting culture volume 1-5 ml 20-50 ml 100-200 ml

Elution volume 100 μl 500 μl 1 ml

Expected DNA yield ~ 20 μg ~ 400 μg ~ 1 mg


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Figure 3. Comparison of plasmid transfection efficiency in 293T cells between MagListo™ and a competitor’s product (spin column type).

As a result of transfection efficiency from 293T cell 1x106, all plasmids extracted from Bioneer MagListoTM and other product showed the efficiency of more than 95%.

Figure 4. Comparison cell viability after transfected with prep samples by MagListo™ and a competitor’s product (spin column type).

The WST assay demonstrates that the cell viability in 293T cell was not affected by plasmid DNA of up to 400 ng/well (6 well plate) extracted respectively by MagListo™ and a competitor’s kit

■ Procedure



K-3600 MagListoTM 5M Plasmid Extraction Kit, 500 rxn in mini

K-3601 MagListoTM 5M Plasmid Extraction Kit, 100 rxn in mini

MagListo™ 5M Plasmid Extraction Kit

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■ DescriptionMagListoTM 5M Gel Extraction Kit is designed to extract fragment DNA from a TAE or TBE agarose gel using Magnetic Nanobeads. With MagListoTM Magnetic Separation Rack and 5M Gel Extraction Kit, it takes only 15 minutes to extract fragment DNA in mini scale even without centrifugation! This kit also provides greater convenience to customers by serving different scales of extraction- mini, midi and maxi - with a single reagent kit. The pH indicator included in the Gel Solubilization Buffer of the kit enables easy optimization for binding condition.

■ Features and Benefits● Only 15 min to purify fragment DNA from an agarose gel using

Magnetic Nanobeads

● No need of expensive extra instrument (vacuum system or air pressure system etc) except MagListoTM Magnetic Separation Rack

■ Specifications

Size range 100 bp -10 kb

Typical recovery 90 -100%


Purification time ~ 15 min

■ ApplicationSubcloning, Sequencing, Labeling, DNA concentration and other molecular biological applications


Figure 1. Comparison of fragment DNA from TBE agarose gel purified with MagListoTM 5M Gel Extraction Kit and competitor’s kit (spin column type).

M: Bioneer 1 kb ladder 1-2: 500 bp DNA purified with Bioneer MagListoTM 5M Gel Extraction Kit3-4: 500 bp DNA purified with competitor Q kit5: Control 500 bp DNA6-7: 3 kb DNA purified with Bioneer MagListoTM 5M Gel Extraction Kit8-9: 3 kb DNA purified with competitor Q kit10: Control 3 kb DNA11-12: 5 kb DNA purified with Bioneer MagListoTM 5M Gel Extraction Kit13-14: 5 kb DNA purified with competitor Q kit15: Control 5 kb DNA

■ Procedure



K-3607 MagListoTM 5M Gel Extraction Kit, 100 rxn in mini

Fast, easy purification of fragment DNA from an agarose gel!

MagListo™ 5M Gel Extraction Kit

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■ DescriptionMagListoTM 5M PCR Purification Kit is designed to purify fragment DNAs of PCR products or other DNA products from various enzymatic reactions including restriction enzyme reaction, A-tailing reaction and labeling reaction. With MagListoTM Magnetic Separation Rack, MagListoTM 5M PCR Purification Kit enables easier and faster purification of fragment DNA in high purity rather than that of a centrifugation, by effective removal of various contaminants in the solution such as dimer, salts, dNTPs, enzymes, mineral oil, dye, detergent, etc.

■ Features and Benefits● Only 5 min for the purification of fragment DNA with the use

of Magnetic Nanobead

● No need of expensive, complicated instrument (vacuum system or air pressure system, etc) except MagListoTM Magnetic Separation Rack

■ Specifications

Size range 100 bp -10 kb

Typical recovery 90 -100%

Expected purity A260/280 > 1.8, A260/230 > 1.6

Purification time ~ 4 min

■ ApplicationSubcloning, Sequencing, Labeling, DNA concentration and other molecular biological applications


Figure 1. Comparison of fragment DNA purified with MagListoTM PCR Purification Kit and control.

A. C: Control 100 bp DNA Ladder (D-1030) 1-2: 100 bp DNA Ladder purified with MagListoTM 5M PCR Purification KitB. C: Control 1 kb DNA Ladder (D-1040) 1-2: 1 kb DNA Ladder purified with MagListoTM 5M PCR Purification Kit

Fast & easy purification of fragment DNAs from PCR product or enzymatic reactant!

MagListo™ 5M PCR Purification Kit

■ Procedure



K-3609 MagListoTM 5M PCR Purification Kit, 100 rxn in mini

A B

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■ DescriptionMagListo™ 5M Viral DNA/RNA Extraction Kit is designed to extract viral DNA/RNA from cell free body fluid (serum, plasma, CSF, saliva, and etc.) and swab, using Magnetic Nanobeads. With MagListo™ Magnetic Separation Rack, MagListo™ 5M Viral DNA/RNA Extraction Kit enables easier and faster extraction of viral DNA/RNA in high purity rather than that of a centrifugation.

■ Features and Benefits● Provided poly (A) can protect RNA degradation during the

extraction steps and enhance the binding efficiency.

● No need of expensive, complicated instrument (vacuum system or air pressure system, etc) except MagListo™ Magnetic Separation Rack

■ Specifications

Sample type Serum, plasma, CSF, saliva, and swab

Starting sample volume Up to 400 μl

Minimum elution volume 50 μl


■ ApplicationPCR, Real–Time PCR


MagListoTM Competitor G

HIV-1 IPC HIV-1 IPC

200IU/ml36.27 26.18 38.51 26.24

36.26 26.88 37.94 27.01

500IU/ml35.23 26.16 37.17 27.22

35.39 26.25 37.21 27.26

1000IU/ml34.66 26.17 36.68 27.01

34.61 26.54 36.03 26.94

Figure 1. Comparison of real-time qPCR data of viral RNA from EDTA human plasma (WHO International Standard 3rd HIV-1,NIBSC code:10/152 200 IU/ml, 500 IU/ml, and 1000 IU/ml) purified with MagListo™ 5M Viral DNA/RNA Extraction Kit and competitor’s kit (spin column type).

Fast & easy extraction of viral DNA/RNA from cell free body fluid (serum, plasma, CSF, saliva, and etc.) and swab!

MagListo™ 5M Viral DNA/RNA Extraction Kit NEW

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■ Procedure



K-3617 MagListoTM 5M Viral DNA/RNA Extraction Kit, 100 rxn in mini

MagListo™ 5M Viral DNA/RNA Extraction Kit NEW

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■ DescriptionMagListo™ 5M Circulating Cell Free DNA Extraction Kit is designed to extract circulating cell free DNA from whole blood (plasma, serum), urine, etc, using Magnetic Nanobeads. With MagListo™ Magnetic Separation Rack and MagListo™ 5M Circulating Cell Free DNA Extraction Kit, it takes only five minutes to extract circulating cell free DNA in mini scale even without centrifugation! This kit enables easier and faster extraction of viral DNA/RNA in high purity rather than that of a centrifugation.

■ Features and Benefits● Takes only 5 minutes in mini, 10 minutes in midi and 15 minutes

in maxi scale (Hands-on time)

● A single kit serves all scales of experiments: mini, midi and maxi


■ ApplicationPCR, Real-Time PCR, Southern Blot, Next-generation, and sequencing


Figure 1. Comparison of circulating cell free DNA from normal human serum (Milipore, 2 ml) purified with MagListoTM 5M Circulating Cell Free DNA Extraction Kit and competitor’s kit (magnetic bead type).

M: Bioneer 25/100bp mixed ladder1-3: Circulating Cell Free DNA of serum purified with Bioneer MagListoTM Yield; 32.4 ng4-6: Circulating Cell Free DNA of serum purified with competitor T kit Yield; 31.6 ng

Figure 2. Comparison of circulating cell free DNA from EDTA human plasma (Seracare Life Science, 2 ml) with Bioneer ladder purified with MagListoTM

5M Circulating Cell Free DNA Extraction Kit and competitor’s kit (magnetic bead type).

M: Bioneer 25/100 bp mixed ladder1-3: Circulating Cell Free DNA of plasma purified with Bioneer MagListoTM 4-6: Circulating Cell Free DNA of plasma purified with competitor T kit

Fast & easy extraction of circulating cell free DNA from whole blood (Plasma, Serum), Urine, and etc.!

MagListo™ 5M Circulating Cell Free DNA Extraction KitNEW

■ Specifications

Sample type Mini scale (Typical yield) Midi scale (Typical yield) Maxi scale (Typical yield)

Plasma, Serum, Urine 1 ml (< 20 μg) 3 ml (< 40 μg) 5 ml (< 80 μg)


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■ Procedure



K-3619 MagListoTM 5M Circulating Cell Free DNA Extraction Kit, 50 rxn in mini

MagListo™ 5M Circulating Cell Free DNA Extraction KitNEW

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■ DescriptionMagListo™ Magnetic Separation Rack is a novel, innovative extraction tool that is designed for nucleic acid extraction or protein purification using Magnetic Nanobeads. Since the rack utilizes magnetic method, extraction and purification are much faster and easier compared to that of column method. A silicone rubber inside the rack tightly holds a tube so that a user can just invert it to discard waste solution from the tube. Select an

appropriate MagListo™- rack among MagListo™-8Ch (8-channel multi-pipette), MagListo™-2 (2 ml, mini), MagListo™-15 (15 ml, midi), MagListo™-50 (50 ml, maxi), and MagListo™-96 (96-well plate) according to the needed amount of sample.

■ Features and Benefits● Save your time via a fast extraction and purification using

Magnetic Nanobeads! Ex) Plasmid DNA prep takes only 5 min for mini and 15 min for midi and 15 min for maxi-scale

● Obtain final products of high purity comparable to any other in the market

● No need of centrifugation● A silicone rubber inside the rack tightly holds a tube. Just

invert it to discard waste solution from the tube

Innovative separation device for nucleic acid extraction and protein purification without centrifuge

MagListo™ Magnetic Separation Rack

■ Specifications

MagListoTM-8Ch Magnetic Separation Rack

Composition Stand 1 ea / Magnet Plate 1 ea

Size (mm)Stand: 104(W) x 23(D) x 31(H)

Magnet Plate: 104(W) x 8(D) x 23(H)

Weight (g) 105

Volume 1 ml tube x 8 positions

MagListoTM-2 Magnetic Separation Rack



Magnet Plate: 136(W) x 9.5(D) x 25(H)

Weight (g) 150





Magnet Plate: 164(W) x 11(D) x 58.5(H)

Weight (g) 525




Size (mm)Stand: 154(W) x 47.5(D) x 76(H)

Magnet Plate: 154(W) x 11(D) x 58.5(H)

Weight (g) 530


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TM-1000 MagListoTM-8Ch Magnetic Separation Rack

TM-1010 MagListoTM-2 Magnetic Separation Rack




TM-1100 MagListoTM Magnetic Separation Rack Bundle Set, Each of MagListoTM-2, -15, -50, and -96 (4 racks)

MagListo™ Magnetic Separation Rack

Plasmid DNA Extraction Kit AccuPrep® Nano-Plus Plasmid Mini Extraction Kit ···························································································· 39

AccuPrep® Nano-Plus Plasmid Midi Extraction Kit ···························································································· 41

AccuPrep® Nano-Plus Plasmid Maxi Extraction Kit ··························································································· 43

AccuPrep® Plasmid Mini Extraction Kit ·················································································································· 45

Genomic DNA Extraction Kit AccuPrep® Genomic DNA Extraction Kit ··············································································································· 47

AccuPrep® GMO DNA Extraction Kit ······················································································································· 49

AccuPrep® Stool DNA Extraction Kit ······················································································································· 51

Viral RNA Extraction Kit AccuPrep® Viral RNA Extraction Kit ·························································································································· 53

Fragment DNA Purification Kit AccuPrep® PCR Purification Kit ·································································································································· 55

AccuPrep® Gel Purification Kit ··································································································································· 57

03

Spin Column Type Manual Nucleic Acid Extraction: AccuPrep®

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■ DescriptionAccuPrep® Nano-Plus Plasmid Mini Extraction Kit extracts highly-purified plasmid DNA from cultured bacterial cells in 10 minutes. The overall principle utilizes a modified alkaline lysis protocol that is enhanced by patented Bioneer’s novel Nano-Technology.

■ Features and Benefits● Principle is based on modified alkaline lysis protocol combined

with a novel nanoparticle technology

● Suitable for high-copy and low copy number plasmid DNA.

● Highly purified and high purity plasmid DNA can be extracted from cultured E. coli cells within 10 min

● Endonuclease A denaturation buffer for the endA+ strains

● Silica based DNA binding column with high DNA binding efficiency

■ Specifications

Starting culture volume 1 ml -10 ml

Column binding capacity > 20 μg


Expected DNA yield ~ 20 μg


■ Application

Subcloning, Sequencing, Transformation, Transfection, and In vitro transcription/translation


Figure 1. Reduced total preparation time.B: AccuPrep® Nano-Plus Plasmid Mini Extraction KitQ, P: Competitors’ plasmid extraction kit

Figure 2. Restriction enzyme digestion test.Electrophoresis results with extracted plasmid DNA after restriction enzyme digestion with various restriction enzymes.

Left: pBlueScript SK(+) vector, Right: pBI121 vectorLane M: Molecular Marker (Cat. no. D-1040, Bioneer).

AccuPrep® Nano-Plus Plasmid Mini Extraction Kit

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■ Procedure



K-3111 AccuPrep® Nano-Plus Plasmid Mini Extraction Kit, 200 rxn

K-3112 AccuPrep® Nano-Plus Plasmid Mini Extraction Kit, 50 rxn

KB-0101 RNase A powder, lyophilized (6 mg/tube)

KA-0033-1 DNA Binding Column Tubes (50 ea X 4 box)

AccuPrep® Nano-Plus Plasmid Mini Extraction Kit

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■ DescriptionAccuPrep® Nano-Plus Plasmid Midi Extraction Kit extractshighly-purified plasmid DNA from cultured bacterial cells in40 minutes. The overall principle utilizes a modified alkalinelysis protocol that is enhanced by patented Bioneer’s novelNano-Technology.



● Suitable for high-copy and low copy number plasmid DNA

● Highly purified and high purity plasmid DNA can be extracted from cultured E.coli cells within 40 min

■ Specifications

Starting culture volume 25 ml - 50 ml


Elution volume 1 ml



■ ApplicationSubcloning, Sequencing, Transformation, Transfection, and In vitro transcription/ translation


Figure 1. Reduced total Preparation time.

B: AccuPrep® Nano-Plus Plasmid Midi Extraction Kit

Q, P: Competitors’ plasmid extraction kit

Figure 2. Restriction enzyme digestion test.

Electrophoresis results with extracted plasmid DNA after restriction.

enzyme digestion with various restriction enzymes.Left: pBluescript SK(+) vector Right: pBI121 vectorLane M: Molecular Marker (Cat. no. D-1040, Bioneer)

AccuPrep® Nano-Plus Plasmid Midi Extraction Kit

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■ Procedure



K-3122 AccuPrep® Nano-Plus Plasmid Midi Extraction Kit, 25 rxn

AccuPrep® Nano-Plus Plasmid Midi Extraction Kit

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■ DescriptionAccuPrep® Nano-Plus Plasmid Midi Extraction Kit extractshighly-purified plasmid DNA from cultured bacterial cells in60 minutes. The overall principle utilizes a modified alkalinelysis protocol that is enhanced by patented Bioneer’s novelNano-Technology.



● Suitable for high-copy and low copy number plasmid DNA

● Highly purified and high purity plasmid DNA can be extracted from cultured E.coli cells within 60 min

■ Specifications

Starting culture volume 100 ml - 250 ml


Elution volume 1 ml



■ ApplicationSubcloning, Sequencing, Transformation, Transfection, and In vitro transcription/translation


Figure 1. Reduced total preparation time.

B: AccuPrep® Nano-Plus Plasmid Maxi Extraction Kit

Q, P: Competitors’ plasmid extraction kit


Electrophoresis results with extracted plasmid DNA after restriction enzyme digestion with various restriction enzymes.Left: pBluescript SK(+) vector, Right: pBI121 vectorLane M: Molecular Marker (Cat. no. D-1040, Bioneer)

AccuPrep® Nano-Plus Plasmid Maxi Extraction Kit

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■ Procedure



K-3131 AccuPrep® Nano-Plus Plasmid Maxi Extraction Kit, 25 rxn

K-3132 AccuPrep® Nano-Plus Plasmid Maxi Extraction Kit, 10 rxn

AccuPrep® Nano-Plus Plasmid Maxi Extraction Kit

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■ DescriptionAccuPrep® Plasmid Extraction Kit is designed for the rapid extraction of high-purity plasmid DNA from bacterial cultures such as E. coli. As a column-type tube is utilized in the purification process, extraction is carried out in three simple steps of binding/washing/elution, and use of dangerous organic solvents is avoided, for the safe and convenient extraction of high-purity plasmid DNA.

■ Features and Benefits● Silica-based DNA binding column with high DNA binding capacity


● Based on the modified alkaline lysis method

● 50 well tube rack for the storage of the extracted plasmid DNA

■ Specifications



Elution volume 50 -100 μl





Figure 1. Restriction enzyme (Xba I) digestion test.

200 ng of extracted plasmid DNA (pBluescript SK(+)) was digested with Xba I.Lane M: Molecular weight marker (λDNA/Hind III+EcoR I, Cat. no. D-1070, Bioneer)Lane 1: AccuPrep® Plasmid Mini Extraction KitLane 2, 3: competitor’s plasmid extraction kit


200 ng of extracted plasmid DNA (pBluescript SK(+)) was digested with various restriction enzymes. Lane M: Molecular weight marker (λDNA/Hind III+EcoR I, Cat. no. D-1070, Bioneer)K: Kpn I, X: Xho I, SI: Sal I, H: Hind III, E: EcoR I, P: Pst I, Sm: Sma I, B: BamH I, S: Spe I, Xb: Xba I, N: Not I, Sc: Sac I

AccuPrep® Plasmid Mini Extraction Kit

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■ Procedure



K-3030 AccuPrep® Plasmid Mini Extraction Kit, 200 rxn

K-3030-1 AccuPrep® Plasmid Mini Extraction Kit, 50 rxn

KB-0101 RNase A powder, lyophilized (6 mg/tube)

KA-0033-1 DNA Binding Column Tubes (50 ea x 4 boxes)

AccuPrep® Plasmid Mini Extraction Kit

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■ DescriptionAccuPrep® Genomic DNA Extraction Kit can rapidly and conveniently extract genomic DNA from blood, lymphocyte, buffy coat, tissue and cultured cell. This process does not require phenol/chloroform extraction, alcohol precipitation, nor other burdensome steps. Our Kit employs spin column type. Proteins and other contaminants which can inhibit enzyme reaction or PCR are eliminated through series of short wash-and-spin steps. The isolated DNA is ready to use in various applications.

■ Features and Benefits● Highly purified and high yield genomic DNA can be extracted

from whole blood, animal tissue, cultured cells

● Silica-based DNA binding column with high binding efficiency

● Optimized Tissue lysis buffer for efficient lysis of the various tissue samples

■ Specifications

Sample Amount Yield

Whole blood 200 μl 3 - 6 μg

Buffy coat 200 μl 15 - 20 μg

Cultured cells 104 - 108 cells 15 - 20 μg

Mammalian tissue 25 - 50 mg 10 - 15 μg

■ ApplicationGene cloning, PCR, Real-Time PCR, Southern blotting, and SNP genotyping


Figure 1. Gel electrophoresis of whole blood genomic DNA.Lane M1: Molecular weight marker, λ DNA/EcoR l + Hind lll Markers (Cat. no. D-1070, Bioneer)Lane M2: Molecular weight marker, 1 kb ladder (Cat. no. D-1040, Bioneer)Lane 1: whole blood genomic DNA of two individual miceLane 2: whole blood genomic DNA of two individual ratsLane 3: whole blood genomic DNA of two persons100 ng of intact DNA (left) and Hind III (E-1721, Bioneer) enzyme digested

DNA (right)

AccuPrep® Genomic DNA Extraction Kit

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■ Procedure



K-3032 AccuPrep® Genomic DNA Extraction Kit, 100 rxn

KB-0111 Proteinase K powder, lyophilized (25 mg/tube X 2 tubes)

KA-0112 DNA Binding Column Tubes package (100 ea)

AccuPrep® Genomic DNA Extraction Kit

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■ DescriptionAccuPrep® GMO DNA Extraction Kit allows the extraction of DNA from agricultural products such as beans, corn, and rice, or from processed foods such as bean sprouts, tofu and condensed milk, for the detection of GMOs. The kit includes all reagents required for extraction, and in addition uses a column type extraction system to allow a more rapid, more convenient extraction of higher-quality DNA than compared to conventional methods. A high amount of high-purity DNA can be extracted: for example, 20 - 40 µg of DNA for 1 g of powdered bean, with an A260/A280 ratio of over 1.8.

■ Features and Benefits● Highly purified and high yield genomic DNA can be extracted

from various plant samples

● Silica-based DNA binding column with high binding capacity

● Optimized plant lysis buffer for the efficient lysis

■ Specifications

Sample Amount Yield

Soybean 100 mg 2 - 5 μg

Maize 100 mg 1 - 4 μg

Potato 100 mg 1 - 4 μg

■ ApplicationGene cloning, PCR, Quantitative Real-Time PCR, Southern blotting, and SNP genotyping

■ Experimental Data M1 M2 1 2 3 4 5

M 1 2 3 4 5

Figure 1. Genomic DNAs from 0.1 g of soybean, tofu and maize.

Lane M1: Molecular weight marker, λ DNA/EcoR l + Hind lll Markers (D-1070, Bioneer)Lane M2: Molecular weight marker, 1 kb ladder (D-1040, Bioneer)Lane 1: Soybean Lane 2: Tofu Lane 3: Maize100 ng of Intact DNA (left) and Hind III (E-1721, Bioneer) enzyme digested DNA (right)

M1 M2 1 2 3 4 5

M 1 2 3 4 5

Figure 2. PCR results for GMO detection. Lane M: Molecular marker, 1 kb ladder (D-1040, Bioneer) Lane 1-5: PCR results for GMO detection

AccuPrep® GMO DNA Extraction Kit

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■ Procedure



K-3031 AccuPrep® GMO DNA Extraction Kit, 100 rxn



AccuPrep® GMO DNA Extraction Kit

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■ DescriptionAccuPrep® Stool DNA Extraction Kit is designed for the rapid, convenient extraction of DNA from fresh or frozen stool or other samples containing large amounts of material that can inhibit PCR. Using the spin-column method, contaminants and enzyme inhibitors (such as heparin, bilirubin bile salts, porphyrin) are eliminated and high-purity DNA is obtained, ready for use in a variety of applications.

■ Features and Benefits● Highly purified and high purity genomic DNA can be extracted

from various type of stool samples

● Silica based DNA binding column with high DNA binding efficiency

● Optimized stool lysis buffer for the efficient lysis and elimination of impurity

■ Specifications

Sample Amount Yield

Stool 100 mg 2 - 5 μg

■ ApplicationPCR, Real-Time PCR, Southern blotting, SNP genotyping, pathogen characterization, etc.


M1 M2 1 2 3

M 1 2 3 4

Figure 1. Gel electrophoresis of stool genomic DNA extracted by AccuPrep® Stool DNA Extraction Kit.

Lane M1: Molecular weight marker, λ DNA/EcoR l + Hind lll Markers (D-1070, Bioneer) Lane M2: Molecular weight marker, 1 kb ladder (D-1040, Bioneer) Lane 1-3: DNA purified with AccuPrep® Stool DNA Extraction Kit100 ng of Intact DNA (left) and Hind III (E-1721, Bioneer) enzyme digested DNA (right)

M1 M2 1 2 3

M 1 2 3 4

Figure 2. PCR results of H. pylori DNA extracted with AccuPrep® Stool DNA Extraction KitLane M: Molecular weight marker, 100 bp ladder (D-1030, Bioneer)Lane 1-4: PCR results for H. pylori detection

AccuPrep® Stool DNA Extraction Kit

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■ Procedure



K-3036 AccuPrep® Stool DNA Extraction Kit, 100 rxn

KB-1141 Stool lysis buffer (50 ml)



AccuPrep® Stool DNA Extraction Kit

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■ DescriptionAccuPrep® Viral RNA Extraction Kit is designed for the rapid and convenient extraction of viral RNA from cell-free samples as serum, plasma, CSF, urine, etc. This kit can be used for extracting RNA from a variety of RNA virus, such as HIV, HAV, HCV, enteroviruses, but not all viruses can be utilized as a source. Also, as it is difficult to extract only RNA from samples containing both DNA and RNA, a cell-free sample must be used in order to prevent DNA contamination.

■ Features and Benefits● Highly purified and high purity viral RNA can be extracted from

various clinical samples (whole blood, CSF, urine, swab…)

● Silica based RNA binding column with high RNA binding efficiency

● Provided poly (A) can protect RNA degradation during the extraction steps and enhance the binding efficiency

■ ApplicationcDNA synthesis, RT-PCR, RT-qPCR, poly A+ RNA selection, Northern blot analysis, and Pathogen detection

AccuPrep® Viral RNA Extraction Kit

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■ Procedure



K-3033 AccuPrep® Viral RNA Extraction Kit, 100 rxn

KB-0121 Poly (A) powder, lyophilized (2 mg/tube)

KA-1111 RNA Binding Column Tubes package (100 ea)

AccuPrep® Viral RNA Extraction Kit

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■ DescriptionAccuPrep® PCR Purification Kit is designed for the purification of up to 10 µg of DNA fragment from PCR and other enzymatic products within 5 minutes. The size range for effective purification is 100 bp - 10 kb, thus common 20 - 40 mer oligonucleotides are removed. The recovery yield exceeds 70 - 90%. Elution volume can be as little as 30 µl when concentrated product is needed.

■ Features and Benefits● Completely purify the fragment DNA from various enzymatic

reaction products within 5 min

● Highly purified and high yield fragment DNA can be purified from various enzymatic reaction products (restriction enzyme digestion, A-tailing, labeling…)

● Double strand and single strand DNA can be purified with high recovery

● Usable range is 100 bp to 10 kb with 70%-90% recovery

● Silica-based DNA binding column with high binding efficiency

■ Application

Subcloning, Sequencing, Labeling, DNA concentration, etc.


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Size of DNA fragment

200 bp 400 bp 1 kb 3 kb 6 kb

BQ

1.0 kb

M 100 90 80 70 60 50 B B B

Figure 1. Comparison of typical recovery % in PCR purification kits. This figure shows recovery percentage after purification of DNA amplified by PCR. The 70% - 90% of DNA were recovered regardless of DNA size (0.2 - 6.0 kb).B: AccuPrep® PCR Purification Kit (K-3034, K-3034-1), Q: Competitor

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Size of DNA fragment

200 bp 400 bp 1 kb 3 kb 6 kb

BQ

1.0 kb

M 100 90 80 70 60 50 B B B

Figure 2. Recovery analysis of the purified PCR product.Lane M: Molecular weight marker, 100 bp Plus ladder (D-1035, Bioneer)Lane 100: 100% Lane 90: 90%, Lane 80: 80% Lane 70: 70% Lane 60: 60%, Lane 50: 50% Lane B: purified PCR product

AccuPrep® PCR Purification Kit

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■ Procedure



K-3034 AccuPrep® PCR Purification Kit, 200 rxn

K-3034-1 AccuPrep® PCR Purification Kit, 50 rxn


AccuPrep® PCR Purification Kit

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■ DescriptionAccuPrep® Gel Purification Kit is designed for the purification of up to 10 µg of DNA fragment from low-melting, TAE, TBE agarose gel fraction within 15 min. Provided Gel binding buffer can extracts the target DNA fragment from the agarose gel into the solution. The recovery yield exceeds 70%.

■ Features and Benefits● Extraction of fragment DNA from low melting agarose gel as well

as TAE, TBE agarose gel

● DNA purification from 70 bp to 10 kb in various size

● Using silica-based DNA binding column with High binding capacity

● Purification of both double and single strand DNA

● Less than 15 min until the purification process is completed

● Very safety and convenience due to nonuse organic solvent and no process of ethanol precipitation

■ SpecificationsSubcloning, Sequencing, Labeling, DNA concentration, etc.


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Size of DNA fragment70 bp 200bp 400bp 1 kb 3 kb 6 kb

BQ

1.0 kb

M 100 90 80 70 60 50 B B B

Figure 1. Comparison of typical recovery yield between AccuPrep® Gel Purification Kit and competitor. This data shows the superior yield of AccuPrep® Gel PurificationKit as competing products.B: AccuPrep® Gel Purification Kit (K-3035)Q: competitor

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Size of DNA fragment70 bp 200bp 400bp 1 kb 3 kb 6 kb

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Figure 2. Recovery analysis of the purified fragment DNA.10.0 kb of plasmid DNA was purified from agarose gel after restriction enzyme digestion. Lane M: Molecular weight marker, 100 bp Plus ladder (D-1035, Bioneer), Lane 100: 100%, Lane 90: 90%, Lane 80: 80%, Lane 70: 70%, Lane 60: 60%, Lane 50: 50%, Lane B: AccuPrep® Gel Purification Kit

AccuPrep® Gel Purification Kit

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■ Procedure



K-3035 AccuPrep® Gel Purification Kit, 200 rxn

K-3035-1 AccuPrep® Gel Purification Kit, 50 rxn


AccuPrep® Gel Purification Kit

Plasmid DNA Extraction Kit AccuPrep® Plasmid Mini Extraction Kit for 96 Well Vacuum Block ···························································· 60

Genomic DNA Extraction Kit AccuPrep® Genomic DNA Extraction Kit for 96 Well Vacuum Block ························································· 61

Fragment DNA Purification Kit AccuPrep® PCR Purification Kit for 96 Well Vacuum Block ··········································································· 62

BioVac™ Vacuum Manifold Vacuum Manifold ··························································································································································· 63

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96-Well Vacuum Block Type Manual Nucleic Acid Extraction

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■ DescriptionAccuPrep® Plasmid Extraction Kit for Biovac 96 Vacuum Manifold can simultaneously extract plasmid DNA from 96 different samples using a Biovac 96 vacuum manifold (A-9030, Bioneer). The extracted plasmid DNA can be directly used for restriction enzyme digestion, PCR, sequencing, transformation, and cloning, without having to undergo any additional purification steps. The 96 samples can be handled without additional machinery such as a centrifuge. This product is also available to other companies’ vacuum manifold system (Qiagen, Promega or Axygen).

■ Features and Benefits● 96 well binding plates with high binding efficiency


● 96 samples can be handled without additional instruments

● Available to other companies’s vacuum manifold system

■ Specifications


Elution volume 50 -100 μl






K-3030-2 AccuPrep® Plasmid Mini Extraction Kit for 96 Well Vacuum Block, 192 rxn

AccuPrep® Plasmid Mini Extraction Kit for 96 Well Vacuum Block

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■ DescriptionAccuPrep® Genomic DNA Extraction Kit can rapidly and conveniently extract genomic DNA from blood, lymphocyte, buffy coat, tissue and cultured cell. This process does not require phenol /chloroform extraction, alcohol precipitation, nor other burdensome steps. Our kit employs spin column type. Proteins and other contaminants which can inhibit enzyme reaction or PCR are eliminated through series of short wash-and-spin steps. The isolated DNA is ready to use in various applications.

■ Features and Benefits● 96 well binding plates with high binding efficiency ● Endonuclease A denaturation buffer for the endA+ strains● 96 samples can be handled without additional instruments● Available to other companies’s vacuum manifold system

■ Specifications

Sample Amount Yield

Whole blood 200 μl ~ 3 μg

Buffy coat 200 μl ~ 10 μg

Cultured cells 104 - 108 cells ~ 10 μg




K-3032-2 AccuPrep® Genomic DNA Extraction Kit for 96 Well Vacuum Block, 192 rxn

AccuPrep® Genomic DNA Extraction Kit for 96 Well Vacuum Block

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■ DescriptionAccuPrep® PCR Purification Kit for Biovac 96 Vacuum Manifold is a high-throughput PCR purification kit that can purify PCR products and eliminate primers, enzymes, dNTPs, salts and buffer. By using the special Biovac 96 Vacuum Manifold developed by Bioneer, 96 separate PCR products can be simultaneously purified in 25 minutes. This product is also available to other companies’s vacuum manifold system (Qiagen, Promega and Axygen).

■ Features and Benefits● Highly purified and high yield fragment DNA can be purified

from various enzymatic reactants

● Double strand and single strand DNA can be purified with high recovery

● Usable range is 100 bp to 10 kb with 70%-90% recovery

● 96 well binding plates with high binding efficiency

● The 96 samples can be handled without additional instruments

■ ApplicationSubcloning, Sequencing, Labeling, DNA concentration, etc.



K-3034-2 AccuPrep® PCR Purification Kit for 96 Well Vacuum Block, 192 rxn

AccuPrep® PCR Purification Kit for 96 Well Vacuum Block

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■ DescriptionBioneer’s BioVac™ 96 Vacuum Manifold is an experiment tool to extract DNA/RNA/Genomic DNA from 96 samples in a short time. By using BioVac™96 Vacuum Manifold with AccuPrep® 96-well prep Kits, DNA/RNA can be extracted from up to 96 samples at once. Bioneer’s 96 well-type Plasmid DNA Extraction Kit, PCR Purification Kit and Genomic DNA Extraction Kit can be used along with other companies’s 96-well extraction Kit.

■ Features and Benefits● 96 samples can be experimented at same time to obtain results

quickly

● Reproducible and consistent experiment results

● Cost-effective product

■ ApplicationSubcloning, Sequencing, Labeling, DNA concentration, etc.



A-9030 BioVacTM 96 Vacuum Manifold

Cat. no. Kits for BioVacTM Vacuum Manifold

K-3030-2 AccuPrep® Plasmid Mini Extraction Kit for 96 Well Vacuum Block, 192 rxn

K-3032-2 AccuPrep® Genomic DNA Extraction Kit for 96 Well Vacuum Block, 192 rxn

K-3034-2 AccuPrep® PCR Purification Kit for 96 Well Vacuum Block, 192 rxn

BioVacTM 96 Vacuum Manifold

AccuZol™ Total RNA Extraction Solution ·····················································································65

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Solution Type Manual Nucleic Acid Extraction:AccuZol ™

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■ Specifications

Sample type Starting amount Elution volume Typical yield (μg)

Animal tissue

LiverKidneySpleenLungBrain

10 mg 50 μl

50 - 7025 - 4040 - 6015 - 258 -12

Cultured cellHeLaA549PC3

1 x 106 cells 50 μl15 - 3015 - 2515 - 25

Bacterial cell E.coli 1 x 107 cells 50 μl 10 -15

Plant tissue Bean leaf 100 mg 50 μl 30 - 50

Blood Human whole blood 250 μl 50 μl 2 - 3

■ DescriptionAccuZol™ is a ready-to-use reagent for the isolation of total RNA from various sample materials.A monophasic solution of phenol and guanidine-salt inhibits RNase activity, and so the reagent maintains integrity of the RNA during sample homogenization or lysis.AccuZol™ allows you to extract high-yield total RNA with small quantities of starting materials.

■ Features and Benefits● Simplicity: AccuZol™ minimize the steps handle hazardous reagents

so that RNase/DNase contamination keeps to a minimum

● Experiment Time: The procedure for total RNA isolation can be completed in 1 hour

● Sample Source: Tissues and cultured cells originated from human, animals, plant, and bacteria, or blood samples

● Easy Phase Separation: Green colored reagent is useful to distinguish between organic phase and aqueous phase

■ ApplicationcDNA synthesis, Northern blotting, RT-PCR, and RT-qPCR


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Figure 1. Total RNA extracted from animal tissue.

Total RNA was isolated from 10-20 mg of rat tissues and 2 µg of total RNA was loaded per lane on a 1% denaturing agarose gel.Lane 1-2: liver, 3-4: kidney, 5-6: spleen,Lane 7-8: lung, 9-10: brain

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Figure 2. Total RNA extracted from human cell lines.

Total RNA was isolated from 1x106 cultured cells and 1 µg of total RNA was loaded per lane on a 1% denaturing agarose gel.Lane 1-2: HeLa (cervical cancer cells)lane 3-4: A549 (lung cancer cells)lane 5-6: PC3 (prostate cancer cells

AccuZol ™ Total RNA Extraction Solution

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■ Procedure



K-3090 AccuZolTM Total RNA Extraction Reagent, 100 ml

AccuZol ™ Total RNA Extraction Solution

Tissue Homogenization Set ··············································································································68

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■ DescriptionTissue homogenization set is developed to convenient tissue homogenization to extract genomic DNA or total RNA from the animal tissue using specialized Tissue filter tube and Tissue stick by grinding. The Tissue filter tube do not allowed re-use and after the use of the Tissue filter tube, the Tissue filter tube box can used like as sample storage rack.

■ Features and Benefits● Consists of Tissue Mixer, Tissue Stick and Tissue Filter Tube

● Single use, no cross-contamination

● Fibrous-rich sample (heart, skeletal muscle, etc) can also be homogenized

■ ContentsComponents Quantity Note

Tissue Mixer 1 ea

Tissue Stick 1 pack 100 ea/pack

Tissue Filter Tube 2 box 50 ea/box

■ Procedure



K-7030 Tissue Homogenization Set, 100 rxn

K-7031 Tissue Stick, 100 ea

K-7032 Tissue Filter Tube, 100 ea

K-7033 Tissue Mixer

Tissue Homogenization Set

exiprep magnetic bead type manual nucleic acid ......dna/rna extraction kit can automatically...

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