ex vitro rooting and ex vitro acclimation by using the 'float hydroponics' technique -...

8/14/2019 Ex Vitro Rooting and Ex Vitro Acclimation by Using the 'Float Hydroponics' Technique - Alexfiraontheweb

1/10

9/17/13 Ex vi tro rooting and ex vi tro accl imation by using the ' float hydroponics' technique - Alexfi raontheweb

file:///C:/Users/H P/Desktop/home group/BM SINGH/Monal/plant propagation technology/hydroculture/Ex vitro rooting and ex vitro acclimation by using the 'floa

Alexfiraontheweb

Home, sweet home

Ex vitro rooting and ex

vitro acclimation by using

the 'float hydroponics'

technique

Linux installation

Microsoft Office on Ubuntu

Linux in Wine

Quite effective

improvisations

Rooting and acclimation

in immersed cell trays in

open air

Snapshots of Dreamlinux

onmy PC

Snapshots of Linux with

Compiz on my PC

Snapshots of Linux with

lightweight window

managers on m y PC

Snapshots of Lorma Linux

onmy PC

Snapshots of Mepis Linux

onmy PC

Snapshots with installing

Ubuntu Lucid Lynx onmy

PC

Some interesting pictures

of acclimated plants

Some of my cuttings

Some of my cuttings

rooted in jars in water

Some of my cuttings

rooted in water

Some of my Linux

programs

Some snaps hots of Linux

with Compiz and

Virtualbox on my PC

Sitemap

Ex vitro rooting and ex vitro acclimation byusing the 'float hydroponics' technique

Introduction. Ex vitro acclimation is an essential phase in the process of

micropropagation, as it allows in vitro - cultured plants to survive innormal,

natural conditions. Ex vitro acclimation is the gradual adaptation of in

vitro-cultured plants to the normal, natural environment.

The usual, classical methods used for ex vitro acclimation involve the use

of various types of trays or pots filled with a solid substrate (peat, perlite,

vermiculite, sterilized soil or a mixture of some of these components).

Acclimation is done in a more or less confined environment (greenhouse,

tunnel etc.) in which humidity is maintained artificially either by artificial fog

generated using a special installation or by covering the cultures with a

transparent lid that retains humid air around the explants, such preventing

them from drying out. Rooting of in vitro-cultured plantlets can be done either in vitro on media

containing either auxins, e.g indolebutyric acid (IBA) or no growth regulators

at all, or it can be done, depending very much on the species and cultivar,

directly ex-vitro in the potting mix used for acclimation. Such, rooting and

acclimation are done in the same phase. For some species this can be

done, for others it is still impossible.

At the Fruit Research Station of Cluj (www.scdpcluj.ro) a novel ex-vitro

acclimation technique was set up by Alexandru Fira (myself) (email:

[email protected]) and eng. Doina Clapa, PhD (email:

[email protected]), based on hydroculture. We started research on

this topic in August 2008. This technique was mainly applied for theacclimation of plantlets already rooted in vitro.

Our method consists of culturing plantlets rooted in vitro and transferred ex-

vitro in vessels containing a thin layer of ordinary tap water without any

fertilizer and plant growth regulator. Information about this acclimation

technique is provided on this website:

https://sites.google.com/site/alexfirasplaceontheweb/new-ex-vitro-

acclimation-method

Here is another movie of ex vitro rooted and acclimated blackberry on a

floating cell tray:http://www.youtube.com/watch?v=CGBwI_ok1cA

The use of a modified method of 'float hydroponics' for ex-vitro rooting and

acclimation was inspired by the description of the 'float hydroponics' method

used for vegetables and tobacco. Some info about float hydroponics is

presented on these websites:

http://edis.ifas.ufl.edu/hs184

http://www.fshs.org/Proceedings/Password%20Protected/1999%20Vol.%20112/31

315%20(TYSON).pdf

Search this site
http://www.fshs.org/Proceedings/Password%20Protected/1999%20Vol.%20112/313-315%20%28TYSON%29.pdfhttp://sites.google.com/site/alexfiraontheweb/system/app/pages/sitemap/hierarchyhttp://sites.google.com/site/alexfiraontheweb/some-snapshots-of-linux-on-my-pc-1http://sites.google.com/site/alexfiraontheweb/some-snapshots-of-linux-on-my-pc-1http://sites.google.com/site/alexfiraontheweb/some-of-my-linux-programs-1http://sites.google.com/site/alexfiraontheweb/some-of-my-cuttings-rooted-in-waterhttp://sites.google.com/site/alexfiraontheweb/some-of-my-cuttings-rooted-in-waterhttp://sites.google.com/site/alexfiraontheweb/some-of-my-cuttings-rooted-in-jars-in-watermailto:[email protected]://sites.google.com/site/alexfiraontheweb/some-of-my-cuttingshttp://sites.google.com/site/alexfiraontheweb/some-interesting-pics-of-acclimated-plantshttp://sites.google.com/site/alexfiraontheweb/snapshots-with-installing-ubuntu-lucid-lynx-on-my-pchttp://sites.google.com/site/alexfiraontheweb/snapshots-of-mepis-linux-on-my-pchttp://sites.google.com/site/alexfiraontheweb/snapshots-of-lorma-linux-on-my-pchttp://sites.google.com/site/alexfiraontheweb/snapshots-of-linux-with-lightweight-window-managers-on-my-pchttp://sites.google.com/site/alexfiraontheweb/snapshots-of-linux-with-lightweight-window-managers-on-my-pchttp://sites.google.com/site/alexfiraontheweb/snapshots-of-linux-with-compiz-on-my-pchttp://sites.google.com/site/alexfiraontheweb/snapshots-of-dreamlinux-on-my-pchttp://sites.google.com/site/alexfiraontheweb/rooting-and-acclimation-in-immersed-cell-trays-in-open-airhttp://sites.google.com/site/alexfiraontheweb/rooting-and-acclimation-in-immersed-cell-trays-in-open-airhttp://sites.google.com/site/alexfiraontheweb/quite-effective-improvisationshttp://sites.google.com/site/alexfiraontheweb/quite-effective-improvisationshttp://sites.google.com/site/alexfiraontheweb/microsoft-office-on-ubuntu-linux-in-winehttp://sites.google.com/site/alexfiraontheweb/linux-installationhttp://sites.google.com/site/alexfiraontheweb/homehttp://sites.google.com/site/alexfiraontheweb/http://sites.google.com/site/alexfiraontheweb/http://www.youtube.com/watch?v=CGBwI_ok1cAhttp://www.youtube.com/watch?v=CGBwI_ok1cAhttp://www.youtube.com/watch?v=CGBwI_ok1cAhttp://sites.google.com/site/alexfirasplaceontheweb/new-ex-vitro-acclimation-methodmailto:[email protected]://www.scdpcluj.ro/http://www.fshs.org/Proceedings/Password%20Protected/1999%20Vol.%20112/313-315%20%28TYSON%29.pdfhttp://edis.ifas.ufl.edu/hs184http://www.youtube.com/watch?v=CGBwI_ok1cAhttp://sites.google.com/site/alexfirasplaceontheweb/new-ex-vitro-acclimation-methodmailto:[email protected]:[email protected]://www.scdpcluj.ro/http://sites.google.com/site/alexfiraontheweb/system/app/pages/sitemap/hierarchyhttp://sites.google.com/site/alexfiraontheweb/some-snapshots-of-linux-on-my-pc-1http://sites.google.com/site/alexfiraontheweb/some-of-my-linux-programs-1http://sites.google.com/site/alexfiraontheweb/some-of-my-cuttings-rooted-in-waterhttp://sites.google.com/site/alexfiraontheweb/some-of-my-cuttings-rooted-in-jars-in-waterhttp://sites.google.com/site/alexfiraontheweb/some-of-my-cuttingshttp://sites.google.com/site/alexfiraontheweb/some-interesting-pics-of-acclimated-plantshttp://sites.google.com/site/alexfiraontheweb/snapshots-with-installing-ubuntu-lucid-lynx-on-my-pchttp://sites.google.com/site/alexfiraontheweb/snapshots-of-mepis-linux-on-my-pchttp://sites.google.com/site/alexfiraontheweb/snapshots-of-lorma-linux-on-my-pchttp://sites.google.com/site/alexfiraontheweb/snapshots-of-linux-with-lightweight-window-managers-on-my-pchttp://sites.google.com/site/alexfiraontheweb/snapshots-of-linux-with-compiz-on-my-pchttp://sites.google.com/site/alexfiraontheweb/snapshots-of-dreamlinux-on-my-pchttp://sites.google.com/site/alexfiraontheweb/rooting-and-acclimation-in-immersed-cell-trays-in-open-airhttp://sites.google.com/site/alexfiraontheweb/quite-effective-improvisationshttp://sites.google.com/site/alexfiraontheweb/microsoft-office-on-ubuntu-linux-in-winehttp://sites.google.com/site/alexfiraontheweb/linux-installationhttp://sites.google.com/site/alexfiraontheweb/homehttp://sites.google.com/site/alexfiraontheweb/


2/10


file:///C:/Users/H P/Desktop/home group/BM SINGH/Monal/plant propagation technology/hydroculture/Ex vitro rooting and ex vitro acclimation by using the 'floa 2

http://www.simplyhydro.com/free2.htm

http://aesop.rutgers.edu/~horteng/floating_hydroponics.htm

http://www.uky.edu/Ag/NewCrops/brassicamq_tw.htm

There is also an impressive video on Youtube on this topic:

http://www.youtube.com/watch?v=FHBhyqowSEc

Using float hydroponics for ex-vitro acclimation and rooting was also

inspired by a Bulgarian colleague and by one disadvantage of thehydroculture acclimation method: the need to replace and resupply the water

in the acclimation vessels. By using float hydroponics one batch of water can

be used during the entire rooting and acclimation period (1-1.5 month).

A description of our method of using float hydroponics for the ex

vitro rooting and acclimation of in vitro-propagated plants

For ex-vitro rooting, take out the plantlets grown on the multiplication

media, in the multiplication phase. These are shoot clumps (miniature

bushes) proliferated on the multiplcation medium under the effect of

cytokinins. Wash the bases of the clumps in order to remove any remnants ofthe nutritive medium. Cut off the base of the shoot clumps with a pair of sharp

scissors in order to obtain a bunch of separate, individual shoots similar to a

fascia. If the bunch is too thick separate it into smaller bunches. Then, plant

each small bunch into cells in an ordinary cell tray and set the cell tray to float

on the surface of the ordinary tap water in a basin in such a way that the

bunches of shoots are partially immersed in the water. Most of the shoots

should be well rooted in about 1 month. There are no special requirements of

air humidity during the whole acclimation period, the cultures can even stand

in open air ! Some species like some rose cultivars and the blackberry can

withstand bad conditions.

Well-rooted shoots should then be planted into pots containing potting mixand grown further in usual conditions. Dead, moulded or rotten shoots (there

are very few of them) should be removed. Living but non-rooted shoots

should be placed back into the water for rooting.

Here there is a new video showing my colleagues transferring

blackberry ex vitro for rooting and acclimation:

http://www.youtube.com/watch?v=7KNc_VQyzgM

Here there is a video showing some blackberry rooted and acclimated ex

vitro in float hydroponics:http://www.youtube.com/watch?v=cXQDgWsTg7w

Here there is another video of a tray full of blackberry shoots, rooted and

acclimated:

http://www.youtube.com/watch?v=CGBwI_ok1cA

http://www.youtube.com/watch?v=zlvxVvAtRRo&feature=related

For the ex-vitro acclimation of in-vitro-rooted plants transfer ex-vitro
http://www.youtube.com/watch?v=zlvxVvAtRRo&feature=relatedhttp://www.youtube.com/watch?v=CGBwI_ok1cAhttp://www.youtube.com/watch?v=cXQDgWsTg7whttp://www.youtube.com/watch?v=7KNc_VQyzgMhttp://www.youtube.com/watch?v=FHBhyqowSEchttp://www.uky.edu/Ag/NewCrops/brassicamq_tw.htmhttp://aesop.rutgers.edu/~horteng/floating_hydroponics.htmhttp://www.simplyhydro.com/free2.htm


3/10



the plants from the rooting phase, wash the roots with warm tap water in

order to remove the remnants of medium and plant one or more plants into

each cell in the cell tray and set the tray to float in a basin containing ordinary

tap water. There is no need for special conditions of humidity. The plants

should be acclimated in about 1 month of culture and they should be planted

into pots containing potting mix.

Below there is a video showing some acclimated Drosera capillaris. The

tray also contains black currant greenwood cuttings:

http://www.youtube.com/watch?v=6XRx66sod0U

Acclimation in hydroculture was a great succcess for Nephrolepis, also:

http://www.youtube.com/watch?v=kx915TmpQFc

Below I am going to show some pictures of plantlets either rooted ex-vitro

in the floating tray or rooted in vitro and acclimated in the floating tray.

Blackberry in the floating cell tray:
http://sites.google.com/site/alexfiraontheweb/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-technique/dscf0294.jpg?attredirects=0http://www.youtube.com/watch?v=kx915TmpQFchttp://www.youtube.com/watch?v=6XRx66sod0U


4/10



Rose cv. The Fairy rooted in vitro and acclimated in the tray:
http://sites.google.com/site/alexfiraontheweb/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-technique/dscf0267.jpg?attredirects=0


5/10


http://sites.google.com/site/alexfiraontheweb/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-technique/dscf0276.jpg?attredirects=0http://sites.google.com/site/alexfiraontheweb/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-technique/dscf0266.jpg?attredirects=0


6/10



Kalanchoe blossfeldiana acclimated in the floating cell tray:
http://sites.google.com/site/alexfiraontheweb/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-technique/dscf0252.jpg?attredirects=0http://sites.google.com/site/alexfiraontheweb/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-technique/dscf0268.jpg?attredirects=0


7/10



A thornless blackberry variety rooted ex-vitro in the floating cell tray:

Blackberry cv. Thornless Evergreen rooted ex-vitro

in the floating cell tray:
http://sites.google.com/site/alexfiraontheweb/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-technique/dscf0312.jpg?attredirects=0http://sites.google.com/site/alexfiraontheweb/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-technique/dscf0314.jpg?attredirects=0http://sites.google.com/site/alexfiraontheweb/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-technique/dscf0247.jpg?attredirects=0


8/10



A thorny blackberry variety rooted ex-vitro in floatation

hydroculture:
http://sites.google.com/site/alexfiraontheweb/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-technique/DSCF9763.jpg?attredirects=0http://sites.google.com/site/alexfiraontheweb/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-technique/DSCF9761.jpg?attredirects=0http://sites.google.com/site/alexfiraontheweb/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-technique/00012.jpg?attredirects=0


9/10



Images of roots grown into the auxiliary polystyrene floats:
http://sites.google.com/site/alexfiraontheweb/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-technique/DSCF9765-1.jpg?attredirects=0http://sites.google.com/site/alexfiraontheweb/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-technique/DSCF9762.jpg?attredirects=0


10/10


fil ///C /U /H P/D kt /h /BM SINGH/M l/ l t ti t h l /h d lt /E it ti d it li ti b i th 'fl 10

i

I wish you success with applyingthis ex-vitro rooting technique ! Alexandru Fira

Sign in Recent Site Activity Terms Report Abuse Print page | Powe red by Google Sites
http://sites.google.com/http://sites.google.com/site/alexfiraontheweb/system/app/pages/reportAbuse?src=/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-techniquehttp://void%28window.open%28%27http//www.google.com/sites/help/intl/en/terms.html'))http://sites.google.com/site/alexfiraontheweb/system/app/pages/recentChangeshttps://www.google.com/a/UniversalLogin?service=jotspot&continue=http://sites.google.com/site/alexfiraontheweb/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-techniquehttp://sites.google.com/site/alexfiraontheweb/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-technique/DSCF9751.jpg?attredirects=0http://sites.google.com/site/alexfiraontheweb/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-technique/DSCF9753.jpg?attredirects=0http://sites.google.com/site/alexfiraontheweb/ex-vitro-rooting-and-ex-vitro-acclimation-by-using-the-float-hydroponics-technique/DSCF9752.jpg?attredirects=0

ex vitro rooting and ex vitro acclimation by using the 'float hydroponics' technique -...

Documents