enzyme of diagnostic importance presentation by rajesh( vph)
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ENZYMES OF DIAGNOSTIC IMPORTANCE
Submitted by RAJESH KUMAR SAHU MVSc (VPH)
Rajesh kumar sahu
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Enzymes in Major Diagnostic Use
Enzyme Disease
Acid phosphatase Prostate cancerAlkaline phosphatase Liver and bone
diseaseAmylase Acute pancreatitisAspartateaminotransferase Liver and heart
diseaseAlanineaminotransferase Viral hepatitis
Creatininekinase Muscle disorders and myocardial infarction
Lactate dehydrogenase Myocardial infarctionLipase Acute pancreatitis
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ACID PHOSPHATASE(ACP)It is basically phospho-
monoestaraseStored in lysosomeDifferent forms found in different
organsTheir serum level used in
prostate cancerAn assay on the rates of activity
of these enzymes may be used to acertain biological demand for phosphate in soil.
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ALKALINE PHOSPHATASE (AP)It is a glycoprotein with subunit
masses ranging from 40-83 kDaAP found in intestine,kidney,liver
and boneAP found in bile cannalicular
membrane of hepatocytes of healthy dogs and rats
Also sinusoidal surface of human being
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CONTINUE….In man there are two genes coding for
APINTESTINAL AP( IAP)TISSUE UNSPECIFIC AP ( UAP)Increase of serum UAP denotes
cholestasisIn dogs under the influnce of excess
gluco corticoids it produces hyper glycosylated form of IAP called as CAP
CAP present in the serum of hyperadrenal corticism and prolonged ill ness.
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CREATININE KINASE (CK)It has four iso enzymes which are
dimers of two protomers with individual masses of 40 kDa
CK activated by Mg+2Serum CK serves as indicator of
cardiac infarctionBruising, prolonged recumbency,
convulsion, shievering results in incresed CK
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ALANINE AMINO TRANSFERASE
Incresed ALT activity is seen in hepatocyte damage
Human and porcine ALT are relatively stable at room temp but when frozen 60% activity loss
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ASPARTATE AMINO TRANSFERASEAST has two iso-enzymes
cytosolic and mitochondrial which has masses about 92kDa
Presence of AST denotes soft tissue damage,
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SORBITOL DEHYDROGENASEAlso called iditol dehydrogenaseHepatocytes have high SDH
activity and incresed plasma SDH is consistent of hepatocytes damage in many species.
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Lactate dehydrogenaseIt is tetramers of two protomers
with molecular weight 35kDaHamolysis appreciable increses
the LDHAnticoagulant such as EDTA and
oxaltes directly inhibit LDH activity
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Cholinterase (ChE)Has acytocholine as substrateOrgano phosphate
insectisides,nerve gas are the inhibitors
Decresed ChE in acute infection,pulmonary infection,muscular dystrophy,renal disease,insecticide intoxication
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Alpha amylaseCa +2 dependedPancreatic dis-order indiction
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γ-glutamyl transferasePhysiological function is
speculated with glutathione metabolism.
Cholestatic disorder results in incresed serum the enzyme activity.
Also indicator of nephro-toxicityBile duct disease also increses
the enzyme conc
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THANK U
HAVE A NICE DAY