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11Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme

Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions

Biocatalytic Synthesis

enzymatic solutions for chemical problems

Enzyme Engineering & Technology

enzymes designed for industry

science is our success

2Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme

Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions

32Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme

Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions

Contents

Reductive Biotransformations 4-5

Oxidative Biotransformations 6-7

C-C Bond Formation 8-9

C-C Bond Breaking 9

Enzymatic Isomerisation 10

Hydrolysis / Esterification 11-12

Enzymatic Cascades 12-13

Glycozyme Technology 14-15

API Modification 16

In-silico Search for Novel Biocatalysts 17

About acib 18

Contact 19

4Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme

Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions

Reduction of Carboxylic Acids

(CAR = carboxylate reductase, ADH = alcohol dehydrogenase; ChemCatChem 2014, 6, 1089-1095; Biotechnol. J. 2014, 9, 822-843; Chemical Monthly, 2016, 147, 575-578; Adv. Synth. Catal., 2016, in press, DOI: 10.1002/adsc.201600914.)

Reductive Biotransformations

Reduction of Aldehydes and Ketones

(ADH = alcohol dehydrogenase; Angew. Chem., Int. Ed. 2002, 41, 1014; Angew. Chem., Int. Ed. 2008, 47, 714; ChemCatChem 2013, 5, 1744.)

Reduction of C=C-Bonds

(X = electron-withdrawing group; Angew. Chem., Int. Ed. 2007, 46, 3934; Chem. Eur. J. 2012, 18, 10367; Curr. Opin. Chem. Biol. 2007, 11, 203; J. Biotechnol. 2012, 162, 381; Nat. Commun. 2014, 5, 4150.)

Reduction of Imines

(IRED = imine reductase; Adv. Synth. Catal. 2015, 357, 1655.)

54Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme

Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions

Reductive BiotransformationsReductive Amination of Aldehydes and Ketones

(TA = -transaminase; Angew. Chem., Int. Ed. 2008, 47, 9337; Angew. Chem., Int. Ed. 2012, 27, 1; Org. Process Res. Dev. 2013, 17, 751; ACS Catal. 2014, 4, 129.)

Structure of an Ene-Reductase

6Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme

Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions

Oxidation of Alcohols / Aldehydes

(ADH = alcohol dehydrogenase; Angew. Chem., Int. Ed. 2002, 41, 1014; Angew. Chem., Int. Ed. 2008, 47, 714; J. Am. Chem. Soc. 2008, 130, 13969; ChemCatChem 2013, 5, 1744.)

C=C-Bond Cleavage

(Angew. Chem., Int. Ed. 2006, 45, 5201; J. Am. Chem. Soc. 2009, 131, 5368; Chem. Commun. 2012, 48, 3303; Adv. Synth. Catal. 2013, 355, 3321.)

Enzymatic De-Amination

(TA = -transaminase; AO = -amino oxidase; Angew. Chem., Int. Ed. 2008, 47, 9337; Angew. Chem., Int. Ed. 2012, 27, 6817; Org. Process Res. Dev. 2013, 17, 751; ACS Catal. 2014, 4, 129.)

Enzymatic De-Alkylation

Oxidative Biotransformations

Enzymatic Hydration

(Angew. Chem. Int. Ed. 2015, 54, 15051.)

(Angew. Chem., Int. Ed. 2013, 52, 2293.)

76Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme

Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions

Oxidative Biotransformations

Enzymatic Hydroxylation

(Nat. Biotechnol. 2002, 20, 1135.)

Baeyer-Villiger Oxidation

(hFMO = human flavin containing monooxygenase; ACS Chem. Biol., 2016, 11, 1039-1048.)

Oxyfunctionalisation of Amino Acids

Oxidative Decarboxylation

(Angew. Chem. Int. Ed. 2015, 54, 88198822; Chem. Commun., 2016, 51, 1918; Eur. J. Org. Chem., 2016, 24733477.)

(aKG = a-ketoglutarate; AAD = amino acid dioxygenase; Front. Microbiol., 2016, 7, 425.)

8Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme

Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions

Bio-Carboxylation

(Chem. Soc. Rev. 2010, 39, 313; Org. Lett. 2012, 14, 1974; J. Biotechnol. 2013, 168, 264; RSC Adv. 2014, 4, 9673.)

Bio-Friedel-Crafts Acylation

(unpublished results)

Biocatalytic Alkaloid Synthesis

(BBE = berberine bridge enzyme; STR = stricto-sidine synthase; NCS = norcoclaurine synthase; Angew. Chem., Int. Ed. 2011, 50, 1068; Org. Pro-cess Res. Dev. 2013, 17, 751; Angew. Chem., Int. Ed. 2014, 53, 3731.)

Trifluoromethylation

(TFMS = trifluoromethylsulfonic acid; TBHP = tert-butylhydroperoxide; Nat. Commun. 2016, in press, DOI: 10.1038/ncomms13323.)

C-C Bond Formation

98Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme

Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions

Cyanohydrin Synthesis/Henry Reaction

(HNL = hydroxynitrile lyase; Angew. Chem., Int. Ed. 2006, 45, 3454; Adv. Synth. Catal. 2007, 349, 1445; ChemCat-Chem 2015, 7, 325; Angew. Chem., Int. Ed. 2003, 42, 4815; Curr. Biotechnol., 2015, 4, 111-117.)

C-C Bond Formation

Asymmetric Synthesis of Optically Pure -Substituted Carboxylic Acids

(AMDase = arylmalonate decarboxylase; ChemCatChem, 2016, 8, 916; Appl. Microbiol. Biotechnol., 2016, 20, 8621.)

C-C Bond Breaking

10Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme

Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions

Racemization of Arylpropionates

(APR = arylpropionate racemase; ChemBioChem, 2016, 16, 1943; Cat. Sci. Technol., 2016, 6, 4397.)

Enzymatic Isomerisation

Isomerisations of C=C-Bonds

(ChemBioChem, 2012, 13, 23462351.)

Disproportionation: Biocatalytic Cannizzaro Reaction

(ADH = alcohol dehydrogenase; ChemCatChem, 2013, 5, 17441748.)

1110Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme

Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions for chemical problems - Enzyme Engineering & Technology - eynzmes designed for industry - Biocatalytic Synthesis - enzymatic solutions

Hydrolysis of Lactams

(ChemCatChem 2014, 6, 2517.)

Bio-Mitsunobu-Inversion

(Ange