Environmental Protection &Proper Handling of Hazardous and

Biological Waste

FORSCIENCE DEPARTMENTS

2014

You will have three tries to pass the test at the end of

this course. Click on last slide – The Quiz at the left to

go directly to the test. If you do not pass the course in

three tries, contact EHS at x5-4139.

Instructions

CSUEB is required by federal, state, and local regulations

to handle hazardous materials and waste in an

environmentally protective manner. Monetary fines can

be large when these materials are improperly handled. In

some cases criminal charges can be made against

individuals if these laws and regulations are not followed.

This training is designed to provide you the procedures

as they relate environmental regulations to properly

handle hazardous materials and waste in the laboratory.

If you have any questions about handling these

materials, contact the CSUEB EHS Department, x5-4139.

Introduction

Lesson Plan

You’ll learn how to handle hazardous materials and waste on campus so that you protect yourself and our natural resources – air and water. The following topics will be covered:

A. AirB. Sanitary Sewer WaterC. Storm Water ProtectionD. Hazardous WasteE. Biohazardous WasteF. Universal WasteG. Personal Protection in the LabH. Emergency Preparedness and ResponseI. Axiom Eyewash

GoalThese regulations are in place to protect air quality by:

Minimizing volatile organic compounds (VOC) released into the air. VOCs include alcohols and formaldehydes

Controlling ozone depleting chemicals

A. Air Emissions

How?

Keep chemical and hazardous waste containers closed when not in use.

Use closed systems whenever possible.

Evaporating residual solvent from otherwise empty containers is prohibited. Pour off the residue into the appropriate waste container.

Triple rinse and put rinsate in appropriate waste container.

A. Air Emissions

City Sanitary Sewer Ordinance:

No corrosives (pH < 6.0 and >12.0)

No solvents down the drains

No flammables down the drains

No oil

No metals (arsenic, copper, cadmium, lead, mercury, nickel, silver, chromium, zinc)

B. Sanitary Sewer Waste Water

DO NOT DISPOSE OF CHEMICALS IN THIS SINK

What materials are allowed down the drain?

Water

Hand soap

Buffer solution with pH > 6.0 and <12.0

Aspirated cell media with pH > 6.0 and <12.0

Diluted bleach

B. Sanitary Sewer Waste Water

Why the restrictions?

Secondary Treatment Microbes are used In the sewer secondary treatment plant to consume the waste. After secondary treatment, 80-90% of human waste and other solids have been removed or digested. A significant proportion of toxic chemicals are also removed by this process. The treatment plant needs to keep the microbes healthy. Small quantities of hazardous materials can kill the microbes and shutdown the process.

B. Sanitary Sewer Waste Water

NOTHING can go down the storm drain except rainwater.

“Only Rain Goes Down the Drain”

Contact EHS :• See anything other then storm water is going into a storm drain

(immediately)• See anything going on the soil

C. Storm Water

The storm drains must be protected from:

Chemical spills

Oil

Sediment (dirt, concrete, etc.)

C. Storm Water

Goal:

Generators must manage hazardous waste from it’s generation to it’s disposal. This is called cradle to grave. This includes CSUEB.

All waste must be properly disposed and tracked

Some companies and materials are managed cradle to cradle

Perpetual reuse

Recovering valuable material like car batteries

D. Hazardous Waste

What are the work practices when handling hazardous waste?

It can not go down the sanitary sewer drain (sink drain)

Do not evaporate residual solvent into the air, triple rinse

Keep all waste containers closed when not in use

Keep waste streams segregated (we’ll talk more about this)

Do not overfill containers

Anyone generating hazardous waste must have annual training. This includes student assistants, interns, and professors.

Students will receive instructions in their class if they generate waste.

Call EHS if you are planning to generate a new waste stream or need training

D. Hazardous Waste

How can you minimize waste:

Use less material if possible

Substitute a less hazardous materials for a more hazardous materials

Throw non-hazardous waste in non-hazardous waste containers

Order only the material you’ll need, don’t stockpile chemicals

Things the Science Departments are already doing:

Share chemicals when possible

Use non-mercury thermometers

Refuse samples from vendors

Use micro-scale chemistry labs

D. Hazardous Waste

Non-hazardous waste is clean trash not contaminated with chemical, biological, or radiological materials. It includes clean

• Paper

• Cardboard

• Bubble pack, wrappers, etc.

• Glass

It’s important to segregate this clean waste out of the hazardous waste stream. Hazardous waste is more costly to dispose. Much of our non-hazardous waste is recycled.

NON-HAZARDOUSWASTE

Let’s first discuss what non-hazardous waste is.

Place uncontaminated trash with sharp or rough edges in broken

glass bins. These should be clearly labeled for Clean glass

only.

Never place anything with sharp edges in the regular

trash. Custodians could injure themselves when

handling it!

NON-HAZARDOUSWASTE

D. Printer Toner Recycling

• Petri dishes with microbial or cell cultures

• Pipettes used to transfer cells or microbes

• Contaminated gloves

• Chemical containers with residual

• Contaminated glassware and bottles

• Electronics

• Batteries

• Fluorescent bulbs

Hazardous materials must not be placed in non-hazardous waste bins. Hazardous waste includes:

D. Hazardous Waste

Now let’s talk about hazardous waste.

The following categories of hazardous waste are generated at CSUEB. Each will be described with the storage and disposal methods explained.

RCRA (Resource and Conservation Recovery Act)

Biological

Universal

Electronic

Computers

D. Hazardous Waste

If you have laboratory equipment that may be contaminated with hazardous, biological, or radioactive materials, contact EHS to inspect the equipment.

D. Hazardous Waste - Equipment

There are four RCRA* Hazardous Waste Characteristics. RCRA Hazardous Waste has one or more of these characteristics:

*Resource Conservation and Recovery Act (1976)

Corrosives – chemicals with pH <6 and >12

Reactive – unstable chemicals, oxidizers are included

Toxic – Oral LD50 of 2500 mg/kg or less

Flammable –flashpoint of less than or equal to 140ºF

D. RCRA Hazardous Waste

There are two types of locations where RCRA hazardous waste can be accumulated and stored:

1. Satellite Storage Area or Workplace Accumulation Area is where the waste is generated. An example would be your lab.

• Waste can be stored here for up to 9 months starting with the Accumulation Date.

• The Accumulation Date is the day the first drop of waste is put in the container

2. Hazardous Waste Storage for Science is in Annex 55

• Once it’s in this waste area – CSUEB has 90 days to dispose of it

• Generators of the waste are responsible to move the waste from their lab to Annex 55

3.The waste cannot be on the site for more than 365 days

D. RCRA Hazardous Waste

Pick-up

• Hazardous waste contractors pick-up waste from the University about every 2-3 months.

• EHS sends out an e-mail 1 week prior to pick-up.

• Contact EHS anytime if you have a special need.

Annex 55

• Annex 55 should always be locked.

• The key is available at NS131 the College of Science Office.

• You will need to have completed this training to borrow the key.

D. RCRA Hazardous Waste

Satellite and Accumulation Storage Areas Requirements

Containers must have the correct labeling

Waste must be placed in compatible containers

Container must be closed and in good condition, no leaking

containers

No overfilling of containers

Liquids are stored within secondary containers

Secondary containers must be kept dry

Spill materials are available

These location must have documented weekly inspections

If no to any of the above, correct the condition. If you need help, call EHS.

D. RCRA Hazardous Waste

Labeling is most common violation when the EPA or DTSC* inspect businesses. The label must have the following:

1. Generator Information

Name

Address and phone number

EPA ID Number – Concord and Hayward each have it’s own

unique number

2. Chemicals in the waste, without that information we will not know

how to process the waste

3. Accumulation Start Date

4. The day it is moved to Annex 55

* Environmental Protection Agency or Department of Toxic Substance and Control

D. RCRA Hazardous Waste

1. All hazardous waste containers must have this information on every hazardous waste container in your labs.

2. Before placing a container or bag of hazardous waste in the Annex, please complete the date it is moved to the Annex.

3. Chemistry is an exception if they use their department labels.

4. Contact Donna Placzek, EHS if you have questions.

Satellite Hazardous Waste Labeling

HAZARDOUS WASTESATELLITE STORAGE

Generator name:

Contents:

CSU East Bay ◦ 25800 Carlos Bee Blvd ◦ Hayward, Ca 94542

□ Solidx Liquid□ Gas

Hazardous Properties (check all that apply):□ Toxic□ CorrosiveX Ignitable□ Corrosive□ OtherAccumulation Start Date:

_____ /_____ /_____Date Moved to Waste

Storage:

__4__ /__2_ /_2015

Building

Phone number Room Number

4321

Dr. Who NScience

X 1234

MethanolToluenewater

10 31 2014

Example of Satellite Storage or Work Place Accumulation Label

Ethanol, methanol, methyl mercury

Science Building – Room 999

2/14/14 10/14/14

510-885-5555

Address

EPA ID

First drop of chemical

When moved to Annex

D. RCRA Hazardous Waste

WARNING!ETHIDIUM BROMIDE WASTE

H302 Harmful if swallowed.H330 Fatal if inhaled.H341 Suspected of causing genetic defects

Accumulation Start Date ________/________/___________

Ethidium Bromide Waste1.Complete both the green labels2.Place them on the container of ethidium

bromide waste3.All waste should be properly labeled

before putting it in the Annex

Current Lab Chemical Waste Streams (solid and liquid)

Segregate hazardous waste. Mixing hazardous waste streams can greatly increase the cost of mixed waste disposal.

Flammable solvents

Organic solvents (separate chlorinated and non-chlorinated)

Oxidizing liquids and solids

Corrosive (separate acid and base)

Toxic liquids and solids

Solid waste contaminated with chemicals

Acrylamide and ethidium bromide

Unused chemicals

Vacuum oil

D. RCRA Hazardous Waste

D. RCRA Hazardous Waste – Dry waste

Use Compatible Bags (yellow or clear) 6 mil is puncture proof

Use covered, water tight, leak proof secondary containers that are maintained in dry state

D. RCRA Hazardous Waste-Liquid waste

Use Compatible carboys, bottles, jars etc .

Secondary containers must be chemical compatible, kept dry and hold the contents of the largest containers plus 20%

HDPE

Glass

Other information

Segregate hazardous waste.

Mixing hazardous waste streams can greatly increase the cost

of mixed waste disposal.

Ask your supervisor or EHS is you have a disposal question

Empty solid containers – place in appropriate solid waste

stream

Example – ethidium bromide containers should go into the solid

toxic waste stream

D. RCRA Hazardous Waste

D. RCRA Hazardous Waste

DO NOT use biohazard bags for RCRA Waste

Mercury

Contact EHS to pick-up and dispose of any mercury

containing equipment including thermometers.

A mercury spill can be costly and time consuming to clean-

up, if you are not trained, contact EHS to clean-up a mercury

spill.

Our goal is to have a mercury free teaching environment.

D. RCRA Hazardous Waste

Empty broken or unbroken solvent (DMSO, Acetone, Ethanol), and chemical contaminated containers and glassware can be put in these containers.

1. Empty liquid waste into the appropriate liquid waste container.

2. Cross out chemical name and write “EMPTY” on the container.

3. Place in labeled, rigid container.

4. Pails are in certain labs, the drum is in Annex 55.

Broken Glass

D. RCRA Hazardous Waste

Other Hazardous Waste• Oils (vacuum and other)• Paint• Ethylyene glycol• Propylene glycol• UPS batteries

Properly label, segregate, move to Annex 55, and place in a secondary container with compatible materials. Contact EHS if you have any questions.

D. RCRA Hazardous Waste

Other Hazardous Waste

• Gas cylinders-large cylinders are recycled

• Small disposable bottles: Remove regulator;

properly label as empty, segregate, move to

Annex 55, and place in a secondary container

with compatible materials. Contact EHS if

you have any questions.

D. RCRA Hazardous Waste

Biological (Medical) Waste Streams:

• Cultures and stocks

• Sharps, needles, razors

• Used petri dishes

• Contaminated pipettes, gloves, etc

E. Biohazardous Waste

Solid Biological (Medical) Waste Streams:

• Rigid leak proof container

• Label with biohazard warning

• Line with red plastic bag*

• Fill only 2/3s – 3/4s full

• Keep secondary container dry

• Keep covered when not in use

*Red bags are a state requirement. Orange is not allowed.

E. Biohazardous Waste

Rigid Container and Red bags must have the Biohazard Symbol

E. Biohazardous Waste

Sharps or needles:

Use a labeled rigid closeable container for:

• Biological contaminated sharps

• Biological contaminated broken glass

• Nursing class – uncontaminated sharps

• Nursing class - contaminated sharps

E. Biohazardous Waste

Work Practices

• Do not overfill containers

• Do not put nonhazardous or RCRA waste in the biohazardous waste

• Do not place bags of waste on the floor

• Do not place biohazardous waste in Annex 55

Contact Sharon Horgan in microbiology to dispose of biological waste. It must be properly bagged before she will except it

E. Biohazardous Waste

Hayward Science Building

• Contact Sharon Horgan in microbiology to dispose of biological waste.

• It must be properly bagged before she will except it.

Concord Campus

• There are biohazardous waste containers in Microbiology and the Student Health Office. Pick-up depends on the quarter and the activity.

• Contact EHS before disposing.

E. Biohazardous Waste

Batteries Fluorescent bulbs Electronic devices Aerosol cans Electronic devices Pressure and vacuum

gauges Cathode ray tubes

Mercury counterweights and dampers

Dilators and weighted tubing Mercury gas flow regulators Mercury containing switches

Universal waste comes primarily from consumer products containing mercury, lead, cadmium and other substances that are hazardous to human health and the environment. These items cannot be discarded as non-hazardous waste nor disposed of in landfills. The underlined items are the most common on campus:

F. Universal Waste

This is the label that must be used for Universal Waste. It must be completely filled out. Like RCRA hazardous waste, the Accumulation Start Date is the first day waste is placed in the container. Each waste must be segregated into separate containers.

We must dispose of the waste within one year of the Accumulation Start Date.

Next, lets discard some alkaline batteries.

F. Universal Waste

02-27-12

Alkaline Batteries

CSUEB

25800 Carlos Bee

Hayward, CA 94542

Small Batteries – alkaline, NiCad, Li, etc.

1. Tape the cathodes and anodes on the batteries >1.5 volts to prevent fires.

2. Separate alkaline batteries from NiCad and Lithium batteries.

3. These must be stored in rigid containers with a cover.

4. Complete a Universal Waste Label

• Contents

• Accumulation Start Date

• Shipper (CSUEB)

• Shipper’s Address

5. There are two labeled containers for each type of battery in the Annex 55.

6. Contact EHS at x5-4139 if you need a container.

F. Universal Waste

Electronic Waste - any item that has electronics except computer equipment

• Cell phone• Microwave• DVDs• Televisions• Projectors• Cameras • Etc, etc, etc ….

Computers Equipment• Computers and accessories• Computer screens – CRT or LCD

For Disposal Contact Information Technology

For Disposal Contact Matt O’Keefe, Property Coordinator x5-4256

F. Universal Waste

When handling hazardous materials in the lab, the minimum PPE is: Safety Glasses Lab coat Closed toe shoes Long pants Chemical compatible glove

Call EHS if you have questions.

G. Personal Protective Equipment (PPE)

Depending on the hazardous material’s properties and handling procedures, you may want to upgrade to more protective PPE:

Call EHS at x5-4139 if you have questions.

Additional splash protection for eye and face protection wear :

Goggles or Face Shield

G. Personal Protective Equipment (PPE)

Different gloves are needed for different chemicals. Here, at CSUEB, most of the time, nitrile gloves are adequate. When you handle a new chemical, verify what glove you should wear by looking at the manufacturer’s glove chart. Other factors to consider when choosing a Chemical Compatible Glove:

Chemical

Splash protection

Emersion

Glove type

Thickness

G. Personal Protective Equipment (PPE)

Call EHS at x5-4139 if you have questions.

Emergency Response Procedures

Fire Power Outage in lab Chemical spill in lab

H. Emergency Response

Emergency Preparedness:

Shower and eyewash (inspect monthly) Fire extinguisher (inspect monthly) Keep spill kits available

H. Emergency Response

Fire in lab or hood

1. Immediately close the hood sash if safe to do so

2. Evacuate the lab, shutting the lab door

3. Evacuate the building by pulling the Fire Alarm Pull Station as you leave the building

4. Go to your Assembly Area and provide First Responders (UPD, Fire Department, EHS, VTLs) with information about the emergency such as location, cause, injuries, etc.

H. Emergency Response

Power Outage:

1. May result in reduced ventilation in the room and chemical laboratory hoods.

2. If safe to do so, close all chemical containers, especially flammable solvents.

3. Closing of flammable solvent containers will help prevent release of flammable vapor.

H. Emergency Response

Chemical SpillCordon off the area and call UPD. Have UPD call EH&S for clean-up of hazardous materials if:

Spill is greater then 1 liter flammable solvent Injury or chemical exposure Fire or potential for a fire Environmental release Not comfortable cleaning it up

Otherwise use Spill Kit for clean-up and contact EH&S for disposal.

H. Emergency Response

Chemical Spill Kit Contents

Goggles Apron Dust Pan Caution Tape 1 Large Pillow 2 Small Pillows 2 Socks 4 Pairs Nitrile Gloves pH Paper 10 Absorbent Pads 1 Bag Vermiculite 2 6-mil Clear Plastic Bags 1 Hazardous Waste Label 1 Tie Wrap

H. Emergency Response

Biohazard Materials Spill

Put on the required PPE (safety glasses, gloves, etc).

Cordon off the area.

Use disposal/paper towels to soak up most of the blood or bodily fluid. Place towels in leak-proof container (biohazard bag) for disposal.

Cover contaminated surface with a towel and pour on a disinfectant (e.g. 1:10 Bleach) Allow it to sit for 5-10 minutes before wiping down area.

Placed contaminated towels in biohazard bag for disposal.

Bleach corrodes stainless steel, so wipe down stainless with a non-bleach material compatible solution.

Disinfect all equipment that comes in contact with contaminant during cleaning. If it’s a large spill and you use a mop, dispose or disinfect the mop head.

Remove PPE (gloves, etc) and place in biohazard bag for disposal.

Wash hands immediately with soap and water for at least 20 seconds after removing gloves.

After cleanup is complete, take waste to designated disposal locations (South Science 3rd floor or Student Health Center). Wear another gloves to carry the waste.

Wash hands again with soap and water after disposing waste.

H. Emergency Response

Hazardous Chemicals on Skin

1. Rinse skin for a full 15 minutes and remove any contaminated clothing. Seek medical attention if more then minor contact or there is a chemical injury.

2. Inform your supervisor.

3. Contact Jill Millican at x5-4227 for follow-up instructions.

H. Emergency Response

Hazardous Chemicals in Eyes

1. First flush eye(s) with water for at least fifteen minutes. Forcibly hold eye open to rinse, while rotating eyeball.

2. Remove contact lenses while rinsing. Do not reinsert contact lenses.

3. Then call UPD for assistance (if needed) and seek medical attention immediately by going to the St. Rose Occupational Health Clinic. Have someone drive you to the clinic.

4. Contact Jill Millican x5-4227 for follow-up information.

H. Emergency Response

HOW TO USE

The Axion Faucet-mounted Eyewash

Locations

The Axion faucet-mounted eyewashes are located in the following areas:

Science North Room # 312Science North Room # 313

Science South Room # 347Science South Room # 321

Step #1

Turn on Faucet…

Step #2

If eyewash is in faucet mode, rotate head in either direction for eyewash mode.

(Faucet Mode) (Turn Head) (Eyewash Mode)

Step #3

Flush eyes for at least 15 minutes. Move your eyes around by looking up and down and from side to side to help rinse as such of the eyes as possible.

Use your hands to keep the eyelids open.

Step #4

When done, turn eyewash to faucet mode to allow the water to drain.

(Faucet Mode)

If you have any questions, contact EHS at

x 5-2395 or x 5-4139

How well do you know Environmental Protection?

You must get an 80% to pass this course.

EP HazWaste Science Quiz1.quiz

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