engineering proteins that bind, move, make and break dna
Post on 26-Jun-2016
Embed Size (px)
ErratumEngineering proteins that bind, move, make and break DNA$
Cynthia H Collins, Yohei Yokobayashi, Daisuke Umeno andFrances H Arnold
Current Opinion in Biotechnology 2003, 14:371378
0958-1669/$ see front matter 2003 Elsevier Ltd. All rights reserved.
In this article, published in the August 2003 issue ofCurrent Opinion in Biotechnology, the authors discussionof engineering a-helical DNA-binding domains failed tonote earlier reports of engineering proteinDNA interac-tions . In particular, two studies reported altering theDNA-binding specificities of the Mnt repressor  andcatabolite activator protein , using genetic selection
methods, whereas a third described the substitution of anentire a-helix to alter the DNA-binding specificity of the434 repressor protein . The authors regret this signif-icant oversight.
References1. Youderian P, Vershon A, Bouvier S, Sauer RT, Susskind MM:
Changing the DNA-binding specificity of a repressor.Cell 1983, 35:777-783.
2. Ebright RH, Cossart P, Gicquelsanzey B, Beckwith J: Mutationsthat alter the DNA-sequence specificity of the catabolite geneactivator protein of E. coli. Nature 1984, 311:232-235.
3. Wharton RP, Brown EL, Ptashne M: Substituting an a-helixswitches the sequence-specific DNA interactions of arepressor. Cell 1984, 38:361-369.
$ PII of original article: S0958-1669(03)00091-0
www.current-opinion.com Current Opinion in Biotechnology 2003, 14:665