Engineering proteins that bind, move, make and break DNA
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ErratumEngineering proteins that bind, move, make and break DNA$Cynthia H Collins, Yohei Yokobayashi, Daisuke Umeno andFrances H ArnoldCurrent Opinion in Biotechnology 2003, 14:3713780958-1669/$ see front matter 2003 Elsevier Ltd. All rights reserved.DOI 10.1016/j.copbio.2003.10.002In this article, published in the August 2003 issue ofCurrent Opinion in Biotechnology, the authors discussionof engineering a-helical DNA-binding domains failed tonote earlier reports of engineering proteinDNA interac-tions . In particular, two studies reported altering theDNA-binding specificities of the Mnt repressor  andcatabolite activator protein , using genetic selectionmethods, whereas a third described the substitution of anentire a-helix to alter the DNA-binding specificity of the434 repressor protein . The authors regret this signif-icant oversight.References1. Youderian P, Vershon A, Bouvier S, Sauer RT, Susskind MM:Changing the DNA-binding specificity of a repressor.Cell 1983, 35:777-783.2. Ebright RH, Cossart P, Gicquelsanzey B, Beckwith J: Mutationsthat alter the DNA-sequence specificity of the catabolite geneactivator protein of E. coli. Nature 1984, 311:232-235.3. Wharton RP, Brown EL, Ptashne M: Substituting an a-helixswitches the sequence-specific DNA interactions of arepressor. Cell 1984, 38:361-369.665$ PII of original article: S0958-1669(03)00091-0www.current-opinion.com Current Opinion in Biotechnology 2003, 14:665
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By making proteins! DNA contains the instructions for which proteins to make *and complicated instructions on when to make them that we don’t fully.
Cyclic AMP and Phorbol Ester-Stimulated Transcription Mediated by Similar DNA Elements that Bind Distinct Proteins
DNA = DeoxyriboNucleic Acid – stores and transmits the genetic information that tells cells which proteins to make and when to make them. DNA is made.
Chromosomes and chromatin. Chromosomes organize and package genes inside cells Bind packaging proteins to DNA to make it more compact. –Histones +DNA.