Anil Mukund LimayeContact AddressDepartment of Biosciences and BioengineeringIndian Institute of Technology GuwahatiNorth Guwahati, Guwahati 781039Assam, IndiaPhone: +91-0361-258 2218 (O), +91-0361- 258 4218 (R)Mobile: +91-9957569787Email: amul@iitg.ernet.in

PERSONAL Date of Birth: 30th Aug, 1974Place of Birth: Pune, Maharashtra, IndiaSex: MaleCitizenship: IndianHome town: PunePermanent Address: 106/6 Hazra Road, Kolkata 700026, West Bengal, IndiaFather’s Name: Mr Mukund Dattatraya LimayeMother’s Name: Mrs Jayashree Mukund LimayeSpouse’s Name: Mrs Prajakta Anil Limaye

EMPLOYMENT

RESEARCH EXPERIENCE

Assistant ProfessorDepartment of Biotechnology, Indian Institute of Technology GuwahatiNov, 2008 – Mar, 2015

Associate ProfessorDepartment of Biosciences and Bioengineering, Indian Institute of Technology GuwahatiApril, 2015 till present

Research Asociate Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India,Feb, 2007 – Oct, 2008

Visiting FellowTata Institute of Fundamental Research, Mumbai, India Feb, 2006 – Jan, 2007

EDUCATION Ph.D (2006)Department of Molecular Reproduction Development and GeneticsIndian Institute of Science, Bangalore, IndiaThesis: Androgen regulated gene expression in the rat ventral prostate: Differential effects of orchidectomy and flutamide treatment

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M.Tech in Biotechnology (1999) Department of Life Science and Biotechnology Jadavpur University, Kolkata, India

B.Pharm (1997) Department of Pharmaceutical TechnologyJadavpur University, Kolkata, India 12th Standard Board Examination (1993)St Lawrence High SchoolWest Bengal Board of Higher Secondary EducationSubjects: Physics, Chemistry, Maths, Biology, Hindi, English

10th Standard Board Examination (1991)St Lawrence High SchoolWest Bengal Board of Secondary Education

RESEARCH INTEREST

CURRENT RESEARCH

Molecular EndocrinologyReproductive BiologyEndocrine Related CancersGene Expression and Regulation

Molecular investigations into the effect of green tea polyphenol, epigallocatechin gallate, on invasion and metastasis of breast cancer cells

According to global cancer statistics, breast cancer is the leading cause of cancer related death in women. Even in South-East Asian countries, the incidence and mortality associated to breast cancer has surpassed that of cervical cancer and is thus poses a threat to the general well being and health of women. Epidemiological studies have indicated an inverse correlation between green tea and cancer risk, which is supported by a number of studies on in-vivo models of cancers. Our current research is focused on the effect of epigallocatechin gallate, a major catechin found in green tea on molecular pathways involved in tumor invasion and metastasis.

Mechanisms of invasion and metastasis of breast cancers: role of estrogen

Metastasis presents itself as a major hurdle in successful treatment of Breast Cancer. The role of estrogen in the etiology of breast cancers has been established beyond doubt. However, the role of estrogen, if any, in the invasion and metastasis of breast tumors is not completely understood. Based on cues obtained from the literature we are trying to understand the role of estrogen in breast tumor invastion and metastasis.

Estrogen regulation and role of candidate genes in breast cancer development and progression

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PUBLICATIONS

CONFERENCE ABSTRACTS

Our research also aims at understanding the role of Cystatin A, GPR30 and Hox genes in breast cancer development and progression.

1. Mohan C. Manjegowda, Gauri Deb, Neeraj Kumar, Anil M Limaye. Expression profiling of genes modulated by estrogen, EGCG or both in MCF-7 breast cancer cells. Genomics Data. 2015 5: 210-212

2. Prasad S, Srikanth K, Limaye AM, Sivaprakasam S. Homo-fermentative production of D-lactic acid by Lactobacillus sp. employing casein whey permeate as a raw feed-stock. Biotechnol Lett. 2014 Feb 22. [Epub ahead of print] PubMed PMID:24563313.

3. Deb G, Thakur VS, Limaye AM, Gupta S. Epigenetic induction of tissue inhibitor of matrix metalloproteinase-3 by green tea polyphenols in breast cancer cells. Mol Carcinog. 2014 Jan 31. doi: 10.1002/mc.22121. [Epub ahead of print] PubMed PMID: 24481780.

4. Khan AA, Singh AK, Bankar RS, Dutta P, Limaye AM. Production of extracellular proteases by Bacillus cereus (Strain PD1) grown on pH adjusted whey. Journal of Pure and Applied Microbiology. Volume: 6 Issue: 2 Pages: 737-746 Published: JUN 2012

5. Limaye AM, Desai KV, Chavalmane AK, Kondaiah P. Regulation of mRNAs encoding MMP-9 and MMP-2, and their inhibitors TIMP-1 and TIMP-2 by androgens in the rat ventral prostate. Mol Cell Endocrinol. 2008 Nov 6;294(1-2):10-8. doi:10.1016/j.mce.2008.07.003. Epub 2008 Jul 15. PubMed PMID: 18675881.

6. Limaye AM, Asangani I, Kalyani T, Kondaiah P. Changes in gene expression following androgen receptor blockade is not equivalent to androgen ablation bycastration in the rat ventral prostate. J Biosci. 2008 Jun;33(2):209-20. PubMed PMID: 18535355.

7. Arany PR, Nayak RS, Hallikerimath S, Limaye AM, Kale AD, Kondaiah P. Activation of latent TGF-beta1 by low-power laser in vitro correlates with increased TGF-beta1 levels in laser-enhanced oral wound healing. Wound Repair Regen. 2007 Nov-Dec;15(6):866-74. PubMed PMID: 18028135.

8. Limaye AM, Asangani I, Bora N, Kondaiah P. Novel flutamide regulated genes in the rat ventral prostate: differential modulation of their expression by castration and flutamide treatments. Asian J Androl. 2007 Nov;9(6):801-8. PubMed PMID: 17968466.

1. Gauri Deb, Sahil Batra and Anil M Limaye. EGCG inhibits MMP-2 and MMP-9 activity by direct binding. Presented in the 31st Annual Convention of Indian Association for Cancer Research and International Symposium on Cancer Genomics and its impact in the Clinic, held in Mumbai (2011)

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2. Abrar Ali Khan, Anup K Singh, R Bankar, P Dutta and Anil M Limaye. Extracellular protease production by a local soil isolate of Bacillus cereus grown in pH adjusted whey. Presented in the 52nd Annual Conference of Association of Microbiologists of India (AMI); International Conference on Microbial Biotechnology for Sustainable Development, held in Chandigarh (2011)

3. Anup K Singh, R Bankar, P Dutta and Anil M Limaye. Extracellular protease production by a local soil isolate of Bacillus cereus grown in pH adjusted whey. – presented in the Gordon Research Conference on Applied and Environmental Microbiology: Functional Interactions from Molecules to Biomes, held in Mt Holyoke College, South Hadley, MA, USA (2011)

4. Gauri Deb and Anil M Limaye. Effect of EGCG on MCF-7 cell proliferation and gelatinolytic activities. 30th Annual Convention of Indian Association for Cancer Research held at Indian Institute of Chemical Biology, Kolkata, India. Feb 6-9th 2011.

5. Anup K Singh, Ritesh S Bankar, Priyanka Dutta and Anil M Limaye. Isolation and characterization of soil bacteria capable of producing extracellular proteolytic enzymes. 79th Annual Meeting of the Society of Biological Chemists (India) held at Indian Institute of Science, Bangalore, India. Dec 13-15th 2010

6. Anil M Limaye, K V Desai, C K Aravinda and Paturu Kondaiah. Regulation of mRNAs encoding MMP-9 and MMP-2 and their inhibitors TIMP-1 and TIMP-2 by androgens in the rat ventral prostate. 28 th Annual Convention of Indian Association for Cancer Research and International Symposium on Emerging Challenges and Approaches in Cancer Biology held in Indian Institute of Science Bangalore, India (2009)

7. Paturu Kondaiah, Anil M Limaye, K Ravi Kiran Reddy, Animesh Agarwal, Namrata Bora and Irfan Ahmed. Complexities in the regulation of gene expression by androgens in the rat ventral prostate. International Symposium for Steroid Hormone Receptor Superfamily and Molecular Signaling held in Thiruvananthapuram (2004)

8. Aravinda CK, Shailaja Devi MM, Anil M Limaye and Paturu Kondaiah. Characterization of a novel, putative androgen regulated integral membrane protein cDNA from rat epididymis. 14th Annual Meeting of the Indian Society for the Study of Reproduction and Fertility held in Bangalore (2004)

9. Paturu Kondaiah, Anil M Limaye, Ravi Kiran Reddy K, Animesh Agrawal, Namrata Bora, Shailaja Devi MM and Aravinda CK. Differential gene expression by androgen ablation following orchidectomy or flutamide in the rat ventral prostate and epididymis. 14th Annual Meeting of the Indian Society for the Study of Reproduction and Fertility held in Bangalore (2004)

10. Anil M Limaye, K Ravi Kiran Reddy, Namrata Bora and Paturu Kondaiah. Expression profiling using Microarray reveals differential regulation in the

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PROFESSIONAL AFFILIATIONS

AWARDS AND HONOURS

rat ventral prostate by orchidectomy and flutamide treatment. 27 th All India Cell Biology Conference held in Pune (2004)

11. Anil M Limaye, Irfan Ahmed, Namrata Bora, K Ravi Kiran Reddy , Animesh Agrawal and Paturu Kondaiah. Expression profiling using microarray and differential display reveal differential gene expression in the rat ventral prostate following orchidectomy and flutamide treatment. International Symposium for Steroid Hormone Receptor Superfamily and Molecular Signaling held in Thiruvananthapuram (2004)

12. Anil M Limaye, Namrata Bora and Paturu Kondaiah. Differential display RT-PCR reveals novel transcripts that show differential regulation by orchidectomy and flutamide treatments. 14th Annual Meeting of the Society for the Study of Reproduction and Fertility held in Bangalore (2004)

13. Anil M Limaye, Namrata Bora and Paturu Kondiah. Differential regulation of gene expression in the rat ventral prostate following orchidectomy and antiandrogen treatment. 10th FAOBMB congress held in Bangalore (2003)

14. Anil M Limaye, Kartiki V Desai and Paturu Kondaiah. Expression of tissue inhibitors of metalloproteinases (TIMPs) in the rat ventral prostate: Regulation by androgens. 26th All India Cell Biology Conference held in Mumbai (2002)

15. Kartiki V Desai, Anil M Limaye and Paturu Kondaiah. Modulation of transforming growth factor-b by androgens. 68th Annual Meeting of the Society of Biological Chemists India held in Bangalore (1999)

Life Member, Indian Association for Cancer Research

Institute Fellowship from the Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India, Feb 2007 – Oct 2008

Visiting Fellowship awarded by Tata Institute of Fundamental Research, Mumbai, India, Feb 2006 – Jan 2007

Fellowship for the PhD degree from Indian Institute of Science, Bangalore, India after clearing the entrance examination, July 1999 – Jan 2006

M.Tech degree scholarship after clearing the entrance test conducted by Jawaharlal Nehru University, July 1997 – June 1999

Organizer’s special prize for best platform presentation at the 27th All India Cell Biology Conference held in Pune, India (2004)

Best poster award at the 14th annual meeting of the Indian Society for the Study of Reproduction and Fertility held in Bangalore, India (2004)

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TEACHING

ADMINISTRATIVE RESPONSIBILITIES

Young investigator award for poster presentation at the International Symposium for Steroid Hormone Receptor Superfamily and Molecular Signaling held at Thiruvanantapuram, India (2004)

Seminar Course for final year B.Tech (Biotechnology) studentsMicroarray Data AnalysisEnzymologyBiochemistryModern BiologyQuantitative BiologyMolecular Biology and Genetic engineering

Departmental level

PastCoordinator, Faculty Recruitment (2011-2012)Member, Departmental Post-Graduate Program CommitteeCoordinator, Entrance Test for Departmental M.Tech Program (2011)

PresentMember, Department Undergraduate Program Committee

Institute Level

PastAssociate Warden, KamengWarden, Dihing

PresentMember, Institutional Biosafety Committe

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