elisa and and and multiplexmultiplex assays assays...elisa kits invitrogen elisa kits allow specifi...

ELISA and multiplex assaysELISA and ELISA and ELISA multiplex assaysmultiplex assaysmultiplex

Protein quantitation guideMultiplex assays for the Luminex platform and ELISA kits

An expansive offering of immunoassays for protein quantitation

The Novex™ brand name has changed to the Invitrogen™ brand with the same products that you know and trust. Look for the packaging to change in mid-2016.

ELISA kits 5

Neurobiology ELISA kits 15

Phosphospecific ELISA kits 16

Antibody pair kits 18

Multiplex assays for the Luminex platform 20

Contents

Characteristics of our antibody pair kits, ELISA kits, and multiplex assay kits

Type of immunoassay

Antibody pair kits (page 18)

ELISA kits (page 5)**

Multiplex assay kits (page 20)

Ready-to-use reagents No; need overnight coating process

Yes Yes

Analytical sensitivity* <10 pg/mL <10 pg/mL <10 pg/mL

Dynamic range* <5–250 pg/mL <5–250 pg/mL <5–2,000 pg/mL

Incubation time* 4 hr 2.5–4 hr 3.5 hr

Multiplexability No No Yes

Number of targets measured/well

1 1 1–50

Readout HRP-TMB (colorimetric) HRP-TMB (colorimetric) RPE (fluorescent)

Instrumentation needed Microplate reader Microplate reader Luminex™ instrument (Luminex™ 100/200™, MAGPIX™, or FLEXMAP 3D™ System)

Instrument read time 2 min 2 min 20–60 min* Every assay has its own specifications. Please consult the protocol for your specific antibody pair, ELISA, or multiplex assay kit.** Values in this table refer to standard colorimetric kits. Ultrasensitive kits are also available.

Highly referenced kits you can trust

Protein targets Species Sample types

• Cytokines

• Chemokines

• Signaling proteins

• Receptors

• Neurobiology markers

• Growth factors

• Adhesion molecules

• Human

• Mouse

• Rat

• Monkey

• Swine

• Bovine

• Serum

• Plasma

• Tissue culture supernatant

• Cell lysate

• Tissue homogenate

• Urine

• Cerebrospinal fluid

• Other sample types

We offer a wide range of immunodetection-based products—in convenient, ready-to-use formats for sensitive and specific detection of intracellular or extracellular proteins. We have developed antibody pair kits, enzyme-linked immunosorbent assay (ELISA) kits for single-analyte analysis, and multiplex assays for multi-analyte

Visit thermofisher.com/immunoassays to find:• Our helpful immunoassay selection guide that allows you to search for assays based on your target protein

• Detailed information on all of our antibody pair kits, ELISA kits, and multiplex assay kits

• Important data demonstrating our assay specificity and sensitivity

analysis. Our kits undergo a rigorous validation process for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency, enabling dependably accurate results. You can use our Invitrogen™ immunoassays to investigate any of these popular specific areas and more:

Our ELISA kits have been referenced in over 3,000 publications and our multiplex assays cited in over 1,000 publications.

4

ELISA kits

Invitrogen™ ELISA kits allow specifi c, quantitative measurements of proteins including cytokines, chemokines, β-amyloids, and signaling targets. Our ELISA kits come ready to use with a precoated 96-well plate and all necessary reagents. A detailed, easy-to-follow protocol with kit-specifi c performance information is also provided. Just add sample, run the assay, and get quantitative results in approximately half a day (Figure 1).

Protein HRP anti–rabbitantibody

Antibody

Incubate standard and sample aspirate and wash 4x

Typical ELISA kit workflow

Incubate detection antibody aspirate and wash 4x

Incubate HRP anti-rabbit antibody aspirate and wash 4x

Incubate stabilized chromogen

Add stop solution and read at 450 nm

Figure 1. Fast and easy, 4-hour typical sandwich ELISA kit protocol. Capture antibodies are precoated on the bottom of the 96-well plate. The sample or standard is added to the wells and incubated to allow target proteins to bind. The wells are then washed to remove unbound material and the detection antibody is added and incubated to form a sandwich around the protein of interest. HRP, conjugated either to anti–rabbit antibody (as shown here) or to streptavidin if the detection antibody is conjugated to biotin, is then added. Next, the chromogen substrate for HRP is added and the subsequent enzymatic reaction turns the solution blue. Finally, the reaction is stopped, turning the solution yellow in proportion to the amount of target protein in the sample. Results are read in a microplate reader at 450 nm.

Each 1-plate kit typically includes: • Antibody-coated 96-well plate

• Detector antibody

• Standard

• HRP conjugate

• Diluent buffers

• Wash buffer

• Chromogen

• Stop solution

• Plate covers

To see all available pack sizes, visitthermofi sher.com/elisa

ELISA kits for protein analysisWatch our online video and learn all about running an ELISA to measure target proteins in serum, plasma, supernatants, cell lysates, and other sample types. ELISA is a widely accepted method for quantifying selected proteins and is often used in conjunction with western blotting to analyze proteins in research samples.

Go to thermofi sher.com/elisa to learn more.

5

Table 2. Recovery testing. The recovery of recombinant human TNFα added to human serum, EDTA plasma, citrate plasma, heparin plasma, and tissue culture medium (RPMI) was measured using the Invitrogen™ Human TNF-α ELISA Kit (Cat. No. KHC3011).

Sample type Average recovery

Serum 92%

EDTA plasma 96%

Citrate plasma 93%

Heparin plasma 88%

RPMI 107%

Table 1. Rigorous assay validation of ELISA kits helps ensure consistent, reliable results.

Specification Description What does it mean for you?

Standard calibration Calibrated to NIBSC (National Institute for Biological Standards and Control), if available

Allows accurate quantitation and consistent standard of reference across multiple platforms

Precision Avg. inter-assay CV <10%; avg. intra-assay CV <10% Consistent results

Sensitivity (Figure 2) Relevant levels of protein Enables detection of low levels of protein

Specificity Cross-reactivity tests are performed with similar analytes

Helps to ensure accurate measurement of the protein of interest

Recovery Buffers are optimized to minimize matrix effects Helps to ensure accurate quantitation of samples within a complex matrix, including serum and plasma

Lot-to-lot consistency (Figure 3)

In-house controls are tested to measure within set ranges

Helps to ensure consistent results with new lots

Linearity of dilution Linear results over the quantitative range of the assay Serial dilution of samples are quantitated accurately

Parallelism (Figure 4) Recombinant protein standards mimic native proteins Samples can be measured with confidence

Our ELISA kits are developed to meet industry-standard specifications including standard calibration, precision, sensitivity, specificity, recovery, lot-to-lot consistency, linearity, and parallelism (Tables 1 and 2; Figures 2–4). We research each protein to target physiologically relevant sensitivity and test in well-established models when available. Our kits are validated on sample types such as serum, plasma, tissue culture supernatant, and cell lysates for signaling or phosphorylated proteins. Our ELISA kit manufacturing includes an ISO 13485 facility with stringent quality control to help provide excellent performance and reproducibility.

ELISA kits (continued)

Our products offer:

• Sensitive, accurate, and consistent performance

• Validation on serum, plasma, tissue culture supernatant, and cell lysates

• Ready-to-use, convenient assay in only half a day

6

Figure 2. Higher sensitivity levels. The standard curves demonstrate the ability to measure lower levels of MMP-9 protein with the Invitrogen™ ELISA Kit (Cat. No. KHC3061) than with a competitor’s kit.

Figure 3. Lot-to-lot consistency. Individual production lots were analyzed using 4 levels of control specimens (C1–C4), which ensured low variation between lots (<20%).

Figure 4. Parallelism testing to ensure recombinant standards are equivalent to natural samples. The data obtained with the recombinant sample standard (blue line) correlated very well with the data from serum, plasma (citrate, heparin, and EDTA) and tissue culture (TC) using the Invitrogen™ Rat IL-6 ELISA Kit (Cat. No. KRC0061), helping to ensure accurate quantitation.

MMP-9 (ng/mL)

0.01 1010.1 100

OD

450

nm

0.5

1.0

1.5

2.0

2.5

3.0

3.5

0

Invitrogen

Competitor

ELISA production lot

A EDCB F G H I J K

IFN

-γ (

IU/m

L)

10

100

1

C1 C2 C3 C4

Concentration (pg/mL)

0 2,0001,000 3,000

OD

450

nm

0.5

1.0

1.5

2.0

2.5

3.0

3.5

0

Standard

Serum

Citrate

Heparin

EDTA

TC

Target Cat. No.

Human6-Ckine (CCL21) EHCCL21

α-fetoprotein EHAFP

Activin A EHACTIVINA

ADAM9 EHADAM9

ADAM11 (MDC) EHADAM11

Adiponectin KHP0041

Aggrecan (PG) KAP1461

AGRP EHAGRP

AHSG (Fetuin A) EHAHSG

Albumin (ALB) EHALB

ALCAM (CD166) EHALCAM

Amphiregulin EHAREG

Angiogenin EHANG

Angiopoietin-like4 EHANGPTL4

Angiopoietin-1 EHANGPT1

Angiopoietin-2 KHC1641

Angiopoietin-4 EHANGPT4

Angiostatin EHANGIOSTATIN

APO-1/FAS/CD95 KHS9501

Apolipoprotein A1 EHAPOA1

Apolipoprotein C1 EHAPOC1



Apolipoprotein E (AD2) EHAPOE

Apolipoprotein E3 EHAPOE3

Apolipoprotein E4 EHAPOE4

APRIL KHC3051

AXL EHAXL

B2M EHB2M

BCAM EHBCAM

BCL2L2 (BCL-W) EHBCL2L2

β-NGF EHNGF

β-secretase 1 EHBACE1

BIRC2 (cIAP2) EHBIRC2

BLC (CXCL13) EHCXCL13

BMP-2 EHBMP2

BMP-4 EHBMP4

BMP-4 (lysates) EHBMP4CL

BMP-5 EHBMP5

BMP-6 EHBMP6

BMP-7 EHBMP7

BTC (Betacellulin) EHBTC

BRAK (CXCL14) EHCXCL14

C5α EHC5A

CA9 EHCA9

CA19-9 EHCA199

Cardiotrophin 1 EHCTF1

CD14 EHCD14

7

Target Cat. No.

Human (continued)CD23 (soluble; FCER2) KAS0251

CD26 (DPP4) EHDPP4

CD40 (TNFRSF5) EHCD40

CD40L (soluble) KHS4001

CD125 (IL5RA) EHIL5RA

CD137 (TNFRSF9) EHTNFRSF9

CD163 (M130) EHCD163

CD200 EHCD200

CD226 (DNAM-1) EHCD226

CEA (CEACAM5) EHCEA

CEACAM1 (BGP) EHCEACAM1

CFD (Adipsin) EHCFD

CHI3L1 (YKL-40) EHCHI3L1

CKMB (CKM/CKB) EHCKMB

Clusterin EHCLU

c-Met (soluble) KHO2031

CNTF EHCNTF

C-Reactive Protein (CRP) KHA0031

CSF1 (M-CSF) EHCSF1

CTACK (CCL27) EHCCL27

CTSS (Cathepsin S) EHCTSS

CXCL16 EHCXCL16

Cystatin C (CST3) EHCST3

DCN (Decorin) EHDCN

DcR3 (TNFRSF6B) EHTNFRSF6B

D-DIMER EHDDIMER

DKK-1 EHDKK1

DKK-3 EHDKK3

DKK-4 EHDKK4

DLL1 EHDLL1

DMP1 EHDMP1

DR5 (TNFRSF10B) EHTNFRSF10B

E-Cadherin 991700

EGF EHEGF

EGF KHG0061

EG-VEGF (PROK1) EHPROK1

E-Selectin (soluble; ELAM-1) KHS2011

ENA-78 (CXCL5) EHCXCL5

ENC1 (CCL28) EHENC1

Endoglin EHENG

Endostatin EHCOL18A1

Eotaxin (CCL11) KAC2231

Eotaxin-2 (CCL24) EHCCL24

EPO Receptor EHEPOR

ErbB2 (HER2) EHERBB2

ErbB3 (HER3) EHERBB3

Estradiol (E2) KAQ0621

Target Cat. No.

FABP2 (intestinal) EHFABP2

FABP3 (cardiac) EHFABP3

FAP EHFAP

Fas Ligand (FasL) KHS9521

FCER2 (CD23) EHFCER2

FcGR2BC (CD32 B/C) EHFCGR2B

Ferritin EHFTL

FETUB (Fetuin B) EHFETUB

FGF1 (FGF-α) EHFGF1

FGF-4 EHFGF4

FGF-4 (lysates) EHFGF4CL

FGF-6 EHFGF6

FGF-7 (KGF) EHFGF7

FGF-9 EHFGF9

FGF-19 EHFGF19

FGF-basic KHG0021

Fibronectin EHFN1

FLT-3 Ligand EHFLT3LG

Fractalkine (CX3CL1) EHCX3CL1

FST (follistatin) EHFST

FSTL3 (FLRG) EHFSTL3

Furin EHFURIN

Galectin-3 EHLGALS3

Galectin-7 EHLGALS7

G-CSF KHC2031

GCP-2 (CXCL6) EHCXCL6

GDF-15 EHGDF15

GDNF EHGDNF

GITR (TNFRSF18) EHTNFRSF18

GITR (TNFRSF18) (lysates) EHTNFRSF18CL

GITRL (TNFSF18) EHTNFSF18

GLP 7-36a EHGLP

Glucagon (GCG) EHGCG

GM-CSF EHGMCSF

GM-CSF KHC2011

Growth Hormone KAQ1081

Growth Hormone EHGH1

HB-EGF EHHBEGF

HCC-1 (CCL14) EHCCL14

HCC-4 (CCL16) EHCCL16

hCG EHCG

HGF KAC2211

HIF-1A EHIF1A

HSPA4 (HSP70) EHHSPA4

HSPB1 (HSP27) EHHSPB1

HTRA2 (OMI) EHHTRA2

HVEM (TNFRSF14) EHTNFRSF14

I-309 (CCL1) EHCCL1


8

Target Cat. No.

ICAM-1 (soluble) KHS5411

ICAM-1 (sCD45) EHICAM1

ICOS (CD278) EHICOS

IFN-α (supernatant) KHC4011

IFN-α (supernatant) 411001

IFN-α (multi-subtype; supernatant) 411051

IFN-α (multi-subtype) 411101

IFN-α (supernatant) 421201

IFN-β (supernatant) 414101

IFN-γ KAC1231

IFN-γ KHC4021

IFN-γ EHIFNG

IFN-ω (omega) 413951

IGF-1R EHIGF1R

IGFBP-1 EHIGFBP1

IGFBP-2 EHIGFBP2

IGFBP-3 EHIGFBP3

IGFBP-4 EHIGFBP4

IGFBP-5 EHIGFBP5

IGFBP-6 EHIGFBP6

IgG (Subclass) (192 assays) 991000

IgG1 EHIGG1

IL-1α EH2IL1A

IL-1β KAC1211

IL-1β EH2IL1B

IL-1β KHC0011

IL-1β (ultrasensitive) KHC0014

IL-1R1 (IL-1RA) EHIL1R1

IL-1R2 (IL-1RB) EHIL1R2

IL-1R4 (IL1RL1) EHIL1RL1

IL-1RA KAC1181

IL-2 EH2IL2

IL-2R EH2IL2R

IL-2RA EHIL2RA

IL-2RB EHIL2RB

IL-3 KHC0031

IL-4 KAC1281

IL-4 EH3IL4

IL-4 KHC0041

IL-5 EHIL5

IL-5 KHC0051

IL-6 KAC1261

IL-6 EH2IL6

IL-6 KHC0061

IL-6R KHR0061

IL-7 EHIL7

IL-8 KAC1301

IL-8 EH2IL8

Target Cat. No.

IL-8 KHC0081

IL-8 (ultrasensitive) KHC0084

IL-10 EHIL10

IL-10 KAC1321

IL-10 KHC0101


IL-10Ra (CD210a) EHIL10RA

IL-10Rb EHIL10RB

IL-11 EHIL11

IL-12 (p40/p70) KAC1561

IL-12 (p40/p70) KHC0121

IL-12 (p70) EHIL12

IL-12 (p70) KAC1568

IL-13 EHIL13

IL-13 KHC0131


IL-13R α2 EHIL13RA2

IL-15 EHIL15

IL-17 KAC1591

IL-17A EHIL17A

IL-17A EHIL17ALPHA

IL-17B EHIL17B

IL-17F EHIL17F

IL-17R EHIL17R

IL-18 KHC0181

IL-18BP EHIL18BP

IL-18RB (IL18RAP) EHIL18RAP

IL-20 EHIL20

IL-21 EHIL21

IL-22 EHIL22

IL-23 (heterodimer) KHC0231

IL-28A EHIL28A

IL-29 (IFNL1) EHIFNL1

IL-33 EHIL33

IL-36G (IL-1F9) EHIL36G

IL-37 (FIL1/IL1F7) EHIL37

Insulin KAQ1251

IP-10 KAC2361

ITAC (CXCL11) EHITAC

ITAC (CXCL11) EHCXCL11

KLK-6 EHKLK6

KLK-10 EHKLK10

KLK-14 EHKLK14

LAG-3 EHLAG3

LDLR EHLDLR

Leptin KAC2281

Leukotriene B4 KHL1741

Leukotriene B4 EHLTB4

9

Target Cat. No.

Human (continued)LH EHLH

LIF/HILDA KAC1351

LIGHT (TNFSF14; HVEM) EHTNFSF14

LIMP-II (SCARB2) EHSCARB2

Lipocalin-2 EHLCN2

LOX-1 (OLR1) EHOLR1

L-Selectin (SELL) EHSELL

LT-α (TNF-β) EHLTA

Lymphotactin XCL1 EHXCL1

LYVE-1 EHLYVE1

Marapsin (PRSS27) EHPRSS27

MBL (oligomer) KIT029

MBL (MBL2) EHMBL2

MCP-1 (CCL2) KHC1011

MCP-2 (CCL8) EHCCL8

MCP-4 (CCL13) EHCCL13

Mer (MERTK) EHMER

MGSA α (CXCL1) EHCXCL1

MICA EHMICA

MICB EHMICB

MIF EHMIF

MIG (CXCL9) EHCXCL9

MIP-1α (CCL3) KAC2201

MIP-1β (CCL4) KAC2291

MIP-1d (CCL15) EHCCL15

MIP-3α (CCL20) EHCCL20

MIP-3α (CCL20) EHCCL20CL

MIP-3β (CCL19) EHCCL19

MMP-1 EHMMP1

MMP-1 EHMMP1CL

MMP-2 KHC3081

MMP-3 KAC1541

MMP-8 EHMMP8

MMP-9 KHC3061

MMP-10 EHMMP10

MMP-13 EHMMP13

MPIF-1 (CCL23) EHCCL23

MSP (MST1) EHMST1

Mucin 1 (CA15-3) EHMUC1

Mucin 16 (CA125) EHMUC16

NAP-2 (PPBP) EHPPBP

N-CAM1 EHNCAM1

Neprilysin (MME) EHMME

NGAL KIT036

NGAL (Rapid) KIT037

NGFR EHNGFR

Nidogen-1 (NID1) EHNID1

Target Cat. No.

NOTCH-1 EHNOTCH1

NOV EHNOV

NPPB (BNP) EHNPPB

NrCAM EHNRCAM

NRG1-b 1 (NRG1) EHNRG1

NT-3 (NTF3) EHNTF3

NT-4 (NTF4) EHNTF4

Oncostatin M (OSM) EHOSM

OPG (TNFRSF11B) EHTNFRSF11B

Osteoactivin (GPNMB) EHGPNMB

Osteocalcin KAQ1381

PAI-1 KHC3071

PAPP-A EHPAPPA

PARC (CCL18) EHCCL18

PARN (DAN) EHPARN

P-Cadherin (CDH3) EHCDH3

PD-1 (PDCD1) EHPDCD1

PDGF-AA EHPDGFA

PDGF-AB EHPDGFAB

PDGF-BB (CSRP2) EHPDGFB

PDGF-BB (lysates; CSRP2) EHCSRP2

PDGFRα EHPDGFRA

PDGFRβ EHPDGFRB

PECAM-1 EHPECAM1

Pepsinogen I (PGI) EHPGI

Pepsinogen II (PGC) EHPGC

Periostin EHPOSTN

PF-4 EHPF4

PGRPs (PGLYRP1) EHPGLYRP1

PLGF (PGF) EHPGF

proBNP (NPPB) EHPRONPPB

Procalcitonin EHPCT

Prostaglandin E2 EHPGE2

Prostaglandin E2 KHL1701

Prostasin (PRSS8) EHPRSS8

PSA (free) (KLK3) EHKLK3F

PSA (total) (KLK3) EHKLK3T

P-Selectin (soluble) KHS2021

RAGE (MOK) EHMOK

RANK (TNFRSF11A) EHTNFRSF11A

RANTES (CCL5) EHRNTS

RARRES2 (TIG2) EHRARRES2

RBP-4 KHP0081

RBP-4 EHRBP4

Resistin KHP0051

S100A8 EHS100A8

SAA (Serum Amyloid A) EHSAA1

SAA (Serum Amyloid A) KHA0011


10

Target Cat. No.

SCF (KITLG) EHKITLG

Sclerostin (SOST) EHSOST

SDF-1α (CXCL12A) EHCXCL12A

SDF-1β (CXCL12B) EHCXCL12B

Serpin A1 EHSERPINA1

Serpin A4 EHSERPINA4

sgp130 (IL6ST) EHIL6ST

ShhN (SHH) EHSHH

Siglec-5 EHSIGLEC5

Siglec-9 EHSIGLEC9

SLAM (SLAMF1) EHSLAMF1

SMAC (DIABLO) EHDIABLO

SPINT1 (HAI-1) EHSPINT1

SPINT2 (HAI-2) EHSPINT2

SSP1 (Osteopontin) EHSPP1

Syndecan 1 (SDC1) EHSDC1

TACE (ADAM17) EHADAM17

TACI (TNFRSF13B) EHTNFRSF13B

TARC (CCL17) EHCCL17

TECK (CCL25) EHCCL25

TFF-3 EHTFF3

TFPI EHTFPI

TGFα EHTGFA

TGFβ RII (TGFBR2) EHTGFBR2

TGFβ2 EHTGFB2

TGFβI (BIGH3) EHTGFBI

TGF-β1 (activated/treated) KAC1688

Thrombospondin 1 EHTHBS1

Thyroglobulin (TG) EHTG

Thyroid Peroxidase EHTPO

Tie-1 EHTIE1

Tie-2 (TEK) EHTEK

TIM-1 (HAVCR1) EHHAVCR1

TIMP-1 KHC1491

TIMP-2 EHTIMP2

TIMP-4 EHTIMP4

TNF-RI (soluble) KAC1761

TNF-RII (soluble) KAC1771

TNF-α KAC1751

TNF-α EH3TNFA

TNF-α KHC3011

TNF-α (ultrasensitive) KHC3014

TP53 (p53) EHTP53

TPO (THPO) EHTHPO

TRAIL R3 TNFRSF10C EHTNFRSF10C

TRAIL R4 TNFRSF10D EHTNFRSF10D

Transferrin EHTF

Trappin-2 (PI3) EHPI3

Target Cat. No.

TREM-1 EHTREM1

TROP1 (EPCAM) EHEPCAM

Troponin I (TNNI3) EHTNNI3

Troponin T (TNNT1) EHTNNT1

TROY (TNFRSF19) EHTNFRSF19

TSH (CGA) EHTSH

TSLP EHTSLP

TWEAK (TNFSF12) EHTNFSF12

Ubiquitin 1 RPS27A EHRPS27A

uPA (PRAP1) EHPRAP1

uPAR (PLAUR) EHPLAUR

Uromodulin (UMOD) EHUMOD

VCAM-1 (soluble) KHT0601

VEGF-165 KHG0111

VEGF-A EH2VEGF

VEGF-A EHVEGFA

VEGF-A (lysates) EHVEGFACL

VEGF-C EHVEGFC

VEGF-D (FIGF) EHFIGF

VEGF R2 (KDR) EHKDR

VEGF R3 (FLT4) EHFLT4

Vitronectin (VTN) EHVTN

vWF EHVWF

WISP-1 EHWISP1

XIAP EHXIAP

MonkeyEGF EPEGF

Flt-3 ligand EPFLT3LG

IL-10 KPC0101

IL-12 (p40/p70) KPC0121

IL-12 (p70) KPC9121

IL-2 KPC0021

IL-4 KPC0041

IL-6 KPC0061

IL-8 KPC0081

PAI-1 (Serpin E1) EPSERPINE1

TNF-α KPC3011

Mouse4-1BB (TNFRSF9) EMTNFRSF9

6-Ckine (CCL21A) EMCCL21A

ACE EMACE

Adiponectin KMP0041

ANGPTL3 EMANGPTL3

AREG EMAREG

Axl EMAXL

BAFF R (TNFRSF13C) EMTNFRSF13C

BIGH3 (TGFBI) EMTGFBI

BLC (CXCL13) EMCXCL13

11

Target Cat. No.

Mouse (continued)BMP-7 EMBMP7

BSSP 4 (PRSS22) EMPRSS22

C5a (HC) EMHC

CCL6 EMCCL6

CD30 (TNFRSF8) EMTNFRSF8

CD30L (TNFSF8) EMTNFSF8

CD36 EMCD36

CD40 EMCD40

CRG-2 (CXCL10) EMCXCL10

G-CSF (CSF3) EMCSF3

GM-CSF (CSF2) EMCSF2

CT-1 (CTF1) EMCTF1

CTACK (CCL27A) EMCCL27A

CTLA-4 EMCTLA4

CXCL15 EMCXCL15

CXCL16 EMCXCL16

Cystatin C (CST3) EMCST3

DAN (PARN) EMPARN

Decorin (DCN) EMDCN

Dkk-1 EMDKK1

DPPIV (DPP4) EMDPP4

E-Cadherin (CDH1) EMCDH1

EGF EMEGF

Endocan (ESM1) EMESM1

Eotaxin-1 (CCL11) EMCCL11

Eotaxin-2 (CCL24) EMCCL24

Epiregulin (EREG) EMEREG

E-Selectin (SELE) EMSELE

Fc γ R2B EMFCGR2B

FGF2 (bFGF) EMFGF2

Flt-3L EMFLT3L

Fractalkine (CX3CL1) EMCX3CL1

Galectin-1 LGALS1 EMLGALS1



GAS6 EMGAS6

GITR L (TNFSF18) EMTNFSF18

GM-CSF EMGMCSF

gp130 (IL6ST) EMIL6ST

Granzyme B (GZMB) EMGZMB

HGF EMHGF

HGF (lysates) EMHGFCL

ICAM-1 (soluble) EMICAM1ALPHA

ICAM-1 (sCD54) EMICAM1

IFN-α KMC4011

IFN-β (supernatant) 424001

IFN-β KMC4041

Target Cat. No.

IFN-γ KMC4021

IFN-γ EM1001

Ig (isotyping) (480 assays) 37503

IgA EMIGA

IgE EMIGHE

IGF-1 EMIGF1

IGF-2 EMIGF2

IGFBP-2 EMIGFBP2

IGFBP-3 EMIGFBP3

IGFBP-5 EMIGFBP5

IGFBP-6 EMIGFBP6

IgG2A EMIGG2A

IL-1RA (IL1RN) EMIL1RN

IL-1α EMIL1A

IL-1α EMIL1ALPHA

IL-1β KMC0011

IL-1β EM2IL1B

IL-2 KMC0021

IL-2 EMIL2

IL-3 EMIL3

IL-4 EMIL4

IL-5 KMC0051

IL-5 EMIL5

IL-5 EMIL5ALPHA

IL-6 KMC0061

IL-6 EM2IL6

IL-6R (IL6RA) EMIL6RA

IL-7 EMIL7

IL-9 EMIL9

IL-10 EM2IL10

IL-10 KMC0101

IL-11 EMIL11

IL-12 (p40/p70) KMC0121

IL-12 (p40/p70) EMIL12TOT

IL-12 (p40) EMIL12P40

IL-12 (p70) KMC9121

IL-12 (p70) EMIL12

IL-12 (p40/p70) IL12B EMIL12B

IL-13 KMC2221

IL-15 EMIL15

IL-17 KMC3021

IL-17A EMIL17A

IL-17B EMIL17B

IL-17E (IL-25) EMIL25ALPHA

IL-18 KMC0181

IL-20 EMIL20

IL-21 EMIL21

IL-28 (IFNL2) EMIFNL2


12

Target Cat. No.

IL-33 EMIL33

Insulin EMINS

I-TAC (CXCL11) EMCXCL11

KC (CXCL1) EMCXCL1

Leptin KMC2281

Lipocalin-2 (LCN2) EMLCN2

LIX (CXCL5) EMCXCL5

L-Selectin (SELL) EMSELL

Lymphotactin XCL1 EMXCL1

MCP-1 (CCL2) KMC1011

MCP-1 (CCL2) EMMCP1

MCP-5 (CCL12) EMCCL12

M-CSF (CSF1) EMCSF1

MDC (CCL22) EMCCL22

MFG-E8 EMMFGE8

MIG (CXCL9) EMCXCL9

MIP-1α (CCL3) EMCCL3

MIP-1γ (CCL9) EMCCL9

MIP-2 (CXCL2) EMCXCL2

MIP-3α (CCL20) EMCCL20

MIP-3β (CCL19) EMCCL19

MMP-2 EMMMP2

MMP-3 EMMMP3

Myeloperoxidase EMMPO

OPG (TNFRSF11B) EMTNFRSF11B

OPN (SPP1) EMSPP1

PAI-1 (Serpin E1) EMSERPINE1

Periostin (Postn) EMPOSTN

PF-4 (CXCL14) EMCXCL14

PLGF-2 (PGF) EMPGF

PRDC (GREM2) EMGREM2

Progranulin (GRN) EMGRN

Prolactin (PRL) EMPRL

pro-MMP-9 EMMMP9

P-Selectin EMSELP

RAGE (STK30) EMSTK30

RANTES (CCL5) KMC1031

Renin 1 (REN1) EMREN1

Resistin (RETN) EMRETN

SAA (Serum Amyloid A) KMA0021

SCF (KITL) EMKITL

SDF-α (CXCL12) EMCXCL12

ShhN (lysates) EMSHHCL

ShhN (SHH) EMSHH

TNFRI (soluble; TNFRSF1A) EMTNFRSF1A

TARC (CCL17) EMCCL17

TCA-3 (CCL1) EMCCL1

TCK-1 (PPBP) EMPPBP

Target Cat. No.

TECK (CCL25) EMCCL25

TIM-1 (HAVCR1) EMHAVCR1

TIMP-1 EMTIMP1

TIMP-1 (lysates) EMTIMP1CL

TIMP-2 EMTIMP2

TNF-α EMTNFA

TNF-α KMC3011

TNFSF4 EMTNFSF4

TPO (THPO) EMTHPO

TRAIL (TNFSF10) EMTNFSF10

TRANCE (TNFSF11) EMTNFSF11

TREM-1 EMTREM1

TSLP EMTSLP

TWEAK (TNFSF12) EMTNFSF12

TWEAK R (TNFRSF12A) EMTNFRSF12A

VCAM-1 EMVCAM1

VEGF KMG0111

VEGF-A EMVEGFA

VEGF-A (lysates) EMVEGFACL

VEGF-B EMVEGFB

VEGFR1 (FLT1) EMFLT1

VEGFR2 (VEGFA) EMVEGFR2

RatActivin-A (INHBA) ERINHBA

Adiponectin KRP0041

BCL-W (BCL2L2) ERBCL2L2

BDNF ERBDNF

β-NGF ERNGF

β-NGF (lysates) ERNGFCL

CINC-1 (CXCL1) ERCXCL1

CINC-3 (CXCL2) ERCXCL2

Clusterin (CLU) ERCLU

CNTF ERCNTF

C-Reactive Protein (CRP) ERCRP

Cystatin C (CST3) ERCST3

EGF EREGF

Fas Ligand (FasL) ERFASLG

Fractalkine (CX3CL1) ERCX3CL1

Fractalkine (lysate; CX3CL1) ERCXCL1CL

Galectin-3 (LGALS3) ERLGALS3

GM-CSF (CSF2) ERCSF2

ICAM-1 ERICAM1

IFN-γ ERIFNGALPHA

IFN-γ ERIFNG

IGF-1 ERIGF1

IGFBP-5 ERIGFBP5

IL-1a ERIL1A

IL-1β ER2IL1B

13

Target Cat. No.

Rat (continued)IL-2 KRC0021

IL-4 KRC0041

IL-6 KRC0061

IL-6 ER3IL6

IL-10 ERIL10

IL-10 KRC0101

IL-12 (p40/p70) KRC0121

IL-12 (p70) KRC2371

IL-13 (lysates) ERIL13

IL-18 KRC2341

Insulin ERINS

Leptin KRC2281

Lipocalin-2 (LCN2) ERLCN2

LIX (CXCL5) ERCXCL5

LIX (lysates; CXCL5) ERCXCL5CL

L-Selectin (SELL) ERSELL

MCP-1 (CCL2) KRC1011

MCP-1 (CCL2) ERMCP1

MIP-2 KRC1021

MMP-8 ERMMP8

PDGF-AA ERPDGFA

Prolactin R ERPRLR

RAGE (AGER) ERAGER

RANTES (CCL5) KRC1031

TCK-1 (PPBP) ERPPBP

TIM-1 (HAVCR1) ERHAVCR1

TIMP-1 ERTIMP1

TIMP-1 (lysates) ERTIMP1CL

TNF-α ER3TNFA

TNF-α KRC3011

VEGF-A ERVEGFA

VEGF-A (lysates) ERVEGFACL

SwineGM-CSF (CSF2) ESCSF2

IFN-γ EPIFNG

IFN-γ KSC4021

IL-1β ESIL1B

Target Cat. No.

IL-4 KSC0041

IL-6 ESIL6

IL-8 KSC0081

IL-10 KSC0101

IL-18 KSC0181

TNF-α KSC3011

TNF-α EP2TNFA

BovineIFN-γ (semi-quantitative) KBC1231

IL-2 EBIL2

TNF-α (TNF) EBTNF

CanineIFN-γ ECIFNG

IL-6 ECIL6

IL-8 (CXCL8) ECCXCL8

IL-10 ECIL10

TNF-α (TNF) ECTNF

EquineIL-1RA (IL1RN) EEIL1RN

IL-2 EEIL2

IL-10 EEIL10

FelineIL-8 (CXCL8) EFCXCL8

RANTES (CCL5) EFCCL5

MultispeciesAKT 62220

cAMP EMSCAMPL

Caspase-3 (cleaved) 62223

cGMP EMSCGMPL

ERK1/2 62206

PARP (cleaved) 62224

PDGF-BB ECPDGFB

S6 62207

S6 62212

STAT3 62214

STAT6 62208

STAT6 62213


Learn more about our standard and ultrasensitive colorimetric ELISA kits at thermofisher.com/elisakits

14

We offer Invitrogen™ neurobiology ELISA kits for accurate and sensitive quantitation of Aβ (β-amyloid) (Table 3), tau (Figures 5 and 6), and α-synuclein to assist researchers studying Alzheimer’s disease, Parkinson’s disease, and other neurodegenerative conditions. The kits offer these advantages to neuroscientists:

• Easy-to-run sandwich ELISA format

• Precoated, 8-well strip–based format

• Consistent, accurate, and sensitive measurements

Neurobiology ELISA kits

Target Cat. No.

HumanAβ40 KHB3481

Aβ42 KHB3441

Aβ42 (ultrasensitive) KHB3544

Aggregated Aβ KHB3491

APP KHB0051

α-Synuclein KHB0061

Tau (total) KHB0041

Tau [pS199] KHB7041

Tau [pS396] KHB7031

Tau [pT181] KHO0631

Tau [pT231] KHB8051

MouseAβ40 KMB3481

Aβ42 KMB3441

Tau (total) KMB7011

Tau [pS199] KMB7041

Table 3. Linearity of ELISA with serially diluted human cerebrospinal fluid. RPMI containing 10% fetal bovine serum was spiked with natural β-amyloid 1-42 from APP-transfected cells and serially diluted in standard diluent buffer over the range of the assay. Linear regression analysis of sample measurements versus the expected concentration yielded a correlation coefficient of 0.99.

Cerebrospinal fluid

DilutionMeasured (pg/mL)

Expected (pg/mL)

Measured (%)

1/4 341.0 341.0 –

1/8 185.4 170.5 108

1/16 94.6 85.3 107

1/32 41.2 42.6 97

Tissue culture supernatant

DilutionMeasured (pg/mL)

Expected (pg/mL)

Measured (%)

1/2 152.1 152.1 –

1/4 76.4 76.0 101

1/8 38.4 38.0 101

1/16 15.2 19.0 80Concentration (pg/mL)

0 1,8001,200600

OD

450

nm

0.4

0.8

1.2

1.6

2.0

2.4

0

Figure 5. Typical 7-point standard curve for the Invitrogen™ Human Tau (total) ELISA Kit (Cat. No. KHB0041). The dynamic range is 31–2,000 pg/mL, with an analytical sensitivity of <12 pg/mL.

Hu tau (pg/mL)

0 1,200400 600 800 1,000200

OD

450

nm

0.5

1.0

1.5

2.0

0

Recombinant Hu tau

Natural Hu tau

Figure 6. Recombinant Hu tau protein standard. Natural human (Hu) tau was serially diluted in standard diluent buffer. The optical density of each dilution was plotted against the standard curve. Parallelism between the natural and recombinant protein indicates that the standard accurately reflects natural Hu tau content in samples.

Learn more about our ELISA kits for neurobiology at thermofisher.com/neuroelisas

15

For studies of intracellular proteins involved in signaling, we offer Invitrogen™ phosphospecific ELISA kits for measurement of total and phosphorylated, modified, or cleavage site–specific proteins. Advantages and benefits of these kits include:

• Specificity—two antibodies directed against the analyte provide better specificity than western blotting

• Sensitivity—more sensitive than western blotting

• Quantitation—get quantitative data in contrast to western blots (Figure 7)

• Medium throughput—96-well format, results in 4 hours, no densitometry analysis needed

Phosphospecific ELISA kits

Figure 7. Confirmation of western blotting data with quantitative results by phosphospecific ELISA assay. (A) Quantitative data obtained using the Invitrogen™ STAT5a [pY694] Human ELISA Kit (Cat. No. KHO0761). (B) Western blotting results using an Invitrogen™ NuPAGE™ gel (Cat. No. NP0321). Assays were performed in parallel.

Protein (µg)0 20102.5 51.250.63 40

OD

450

nm

1.0

2.0

3.0

4.0

0

A

BProtein (µg)

0 20102.5 51.250.63 40

OD

450

nm

1.0

2.0

3.0

4.0

0

A

B

Learn more about our phosphospecific ELISA kits at thermofisher.com/phosphoelisas

A

B

Need an ELISA plate reader or washer? Visit thermofisher.com/microplate-instruments to see our complete workflow solutions.

16

Target Species reactivity Cat. No.

4E-BP1 [pT46] Human, mouse, rat KHO0691

Akt (total) Human, mouse, rat KHO0101

Akt [pS473] Human, mouse, rat KHO0111

Akt1 (total) Human KHO0531

Akt1 [pS473] (ultrasensitive) Human, mouse, rat KHO0541

Akt [pT308] Human, mouse, rat KHO0201

AMPKα [pT172] Human, mouse, rat KHO0651

β-Catenin (total) Human, mouse, rat KHO1211

Caspase-3 (active) Human KHO1091

c-Met (total) Human KHO0251

c-Myc (total) Human KHO2041

CREB (total) Human, mouse KHO0231

CREB [pS133] Human, mouse KHO0241

Cytochrome c Human KHO1051

EGFR (full-length) Human KHR9061

EGFR [pY1068] Human KHR9081

EGFR [pY1173] Human KHR9071

ERK1/2 [pT185/pY187] Human, mouse, rat KHO0091

ERK1/2 [pT202/pY204] EMS2ERKP

FAK (total) Human, mouse, rat KHO0431

FAK [pY397] Human, mouse, rat KHO0441

GSK-3β [pS9] Human, mouse, rat EMS2GSK3P

GSK-3β [pS9] Human, rat EMSGSK3T

GSK-3β (total) Human, mouse, rat KHO0451

GSK-3β [pS9] Human, mouse, rat KHO0461

HER2 (total) Human KHO0701

HSP27 (total) Human KHO0331

IGF-1R [pY1135/pY1136] Human, mouse, rat KHO0501

IκBα [pS32] Human KHO0221

IR (β-subunit) Human, mouse, rat KHR9111

IR [pY1158] Human, mouse, rat KHR9121

IR [pY1334] Human, mouse, rat KHR9161

IR [pYpY1162/pY1163] Human, mouse, rat KHR9131

IRS-1 (total) Human, mouse, rat KHO0511

IRS-1 [pS312] Human, mouse, rat KHO0521

JAK2 (total) Human, mouse KHO5521

JAK2 [pY1007/pY1008] Human, mouse KHO5621

JNK1/2 (total) Human, mouse KHO0121

JNK1/2 [pT183/pY185] EMSJNKP

JNK1/2 [pT183/pY185] Human KHO0131

MEK 1 (total) EMS2MEKT

MEK 1 [pS218/pS222] EMSMEKP

NF-κB p65 (total) Human KHO0371

p21 Waf1/Cip1 (total) Human KHO5421

p38 [pT180/pY182] EMSP38P

p70-S6K (total) Human, mouse, rat KHO0571

PARP (cleaved) [214/215] Human KHO0741

PRAS40 (total) Human KHO0411

Cell/tissue extraction buffers** Quantity Cat. No.

Cell Extraction Buffer 100 mL FNN0011

NP40 Lysis Buffer 100 mL FNN0021

Tissue Extraction Reagent I 100 mL FNN0071

Denaturing Cell Extraction Buffer 100 mL FNN0091

** Cell/tissue extraction buffers are not included in any assay kits.

Target Species reactivity Cat. No.

PRAS40 [pT246] Human KHO0421

PRAS40 [pT246] Mouse KMO0421

PRAS40 (total), mouse Human, mouse, rat KMO0411

PRAS40 [pT246], mouse Human, mouse, rat KMO0421

SMAD2 (total) Human KHO2021

STAT3 [pY705] Human, mouse, rat KHO0481

STAT5a (total) Human, mouse, rat KHO0751

STAT5a [pY694] Human KHO0761

STAT5b [pY699] Human, mouse KHO5721

Tau (total) Human KHB0041

Tau [pS199] Human KHB7041

Tau [pS396] Human KHB7031

Tau [pT181] Human KHO0631

Tau [pT231] Human KHB8051

Tau (total) Mouse KMB7011

Tau [pS199] Mouse KMB7041

17

Antibody pair kits

Invitrogen™ antibody pair kits contain matched, pretitered, and fully optimized capture (coating) and detection antibodies. These kits enable you to build your own ELISA or any other assay platform that utilizes a matched antibody pair. For convenience, we also offer the Invitrogen™ Buffer Kit for Antibody Pairs that contains premade, easy-to-use buffers and solutions that are optimized for use with antibody pair kits.

• Convenient format for maximum flexibility

• Potential cost savings over ready-to-use ELISA kits

• Easy to use with optimized reagents and protocol

Our Buffer Kit for Antibody Pairs (Cat. No. CNB0011) supplies sufficient reagents for 10 ELISA plates and includes:

• Assay buffer (5X)

• Coating buffer A

• Coating buffer B

• Wash buffer (25X)

• Stabilized chromogen (substrate)

• Stop solution

Each kit typically includes: • Capture antibody


• Standard

• Streptavidin-HRP

Buffer kit sold separately.

Learn more at thermofisher.com/buildyourownimmunoassay

18

Buffer kit Quantity Cat. No.

Buffer Kit for Antibody PairsThe Buffer Kit for Antibody Pairs include the following components (sufficient reagents for 10 plates):

Assay Buffer (5X) 200 mL x 1 bottle

CNB0011

Coating Buffer A 100 mL x 1 bottle

Coating Buffer B 100 mL x 1 bottle

Wash Buffer (25X) 100 mL x 3 bottles

Stabilized Chromogen 25 mL x 4 bottles

Stop Solution 100 mL x 1 bottle

Kit components sold individually:

Assay Buffer (5X) 200 mL x 1 bottle DS98200

Stabilized Chromogen 25 mL x 1 bottle SB01

Stabilized Chromogen, Bulk Pack 1 L bottle SB02

Stop Solution, Bulk Pack 1 L bottle SS04

Using antibody pairs to make an ELISAWatch our online video and learn how to use antibody pairs to set up an ELISA, and find out what prematched antibody pairs we offer to help make ELISA analysis easier and more successful.

Visit thermofisher.com/protein to learn more.

Target Quantity Cat. No.

Human

Akt 5 plates CHO0115

c-Met (total) 5 plates CHO0285

c-Met (soluble) 5 plates CHO0315

HIF-1 α 5 plates ESSHIF1A

IFN-γ 5 plates ESS0002

IFN-γ 10 plates CHC1233

IL-1ra 10 plates CHC1183

IL-1β 10 plates CHC1213

IL-2 5 plates ESS0010

IL-2 10 plates CHC1243


IL-6 5 plates ESS0005


IL-8 5 plates ESS0013*



IL-12 (p40/p70) 10 plates CHC1563


IP-10 10 plates CHC2363

MCP-1 (CCL2) 10 plates CHC1013

MIP-1β (CCL4) 10 plates CHC2293

MMP-3 10 plates CHC1543

SAA (Serum Amyloid A) 10 plates CHA2513

TNF-α 10 plates CHC1753

TNF-α 5 plates ESS0001*

VEGF 10 plates CHG0113

VEGF-A 5 plates ESSHVEGF*

Mouse

IFN-γ 10 plates CMC4033

IL-4 10 plates CMC0043






IL-1β 10 plates CMC0813


TNF-α 10 plates CMC3013

Target Quantity Cat. No.

Rat

IL-2 10 plates CRC0023



Swine

IFN-γ 10 plates CSC4033

IL-2 10 plates CSC1243



TNF-α 10 plates CSC1753

Chicken

IFN-γ 10 plates CAC1233

Bovine

IFN-γ 5 plates ESS0026B*

IL-1β 5 plates ESS0027*



Equine

TNF-α 5 plates ESS0017*

Multispecies

TGF-β1 (activated/treated) 10 plates CHC1683

*This kit is optimized for other buffers. See manual for more information.

19

Multiplex assays for the Luminex platform

Measure proteins simultaneouslyInvitrogen™ multiplex assays enable fast and efficient profiling of multiple proteins in a single well using the Luminex™ xMAP™ (multi-analyte profiling) technology. Multiplexing assays provide more data from each sample of plasma, serum, or tissue culture supernatant than do ELISAs, a critical feature when sample size is limited. Most importantly, you are able to achieve a more comprehensive study of protein signaling in your precious samples when multiplexing (Figure 8).

Figure 8. Seven serum samples are analyzed in a single experiment using the Invitrogen™ Cytokine Human Magnetic 25-Plex Panel. Serum samples from seven different individuals were assayed with the Cytokine Human Magnetic 25-Plex Panel (Cat. No. LHC0009M) to determine the levels of 25 different cytokines and chemokines simultaneously. Measurements were performed using the Luminex™ 200 system.

How Luminex technology worksThe Luminex xMAP technology is based on internally color-coded microspheres (beads) containing fluorescent dyes. With varying concentrations of these dyes, as many as 500 distinct color bead sets can be created, each of which can be coupled to a target-specific molecule such as an antibody. Multiple antibody-conjugated beads can then be combined in a single well of a 96-well plate to measure

Antigen-specific capture antibodies are bound to microspheres

Antigen from the test sample is bound to the microspheres

Signal is generated by labeled secondary antibody attachment

Figure 9. Protocol used with Luminex bead-based assays.

multiple targets simultaneously. Once the antigen is bound, a reporter molecule labeled with a different fluorescent dye is introduced. Then, the 96-well plate is placed into the Luminex™ instrument, where the bead set is identified and the fluorescence of the reporter molecule is used to quantify the amount of target molecule (Figure 9).

20

Magnetic vs. nonmagnetic polystyrene beadsWe offer two types of bead kits: magnetic and nonmagnetic. Magnetic beads enable easier wash steps.

Figure 10. Schematic representation of nonmagnetic and magnetic beads. The encapsulated magnetite layers around the polystyrene core account for the larger size of the magnetic bead.

Nonmagnetic Magnetic

Invitrogen™ magnet Competitor magnet

Figure 11. Magnetic bead aggregation comparison. Drawing of one well of a 96-well plate from the top view. Red bar and circles depict where the magnets are located when placing the 96-well plate on the handheld magnet.

Advantages of the handheld Invitrogen™ Magnetic 96-Well Separator (right; Cat. No. A14179)• Visual verifi cation that beads are not

lost after wash steps (Figure 11)

• Strong magnetic fi eld allows for better washes (Figure 12)

Figure 12. The Invitrogen™ handheld magnet is 6x stronger than that of the competitor, allowing for better washing and assay performance. The magnetic fl ux density was measured at the bottom of a well of a 96-well plate placed on top of a magnet. Courtesy image and analysis from Dexter Magnetic Technologies, Inc.

Invitrogen magnet Competitor magnet

Watch how to use the handheld magnet for magnetic assays. Go to thermofi sher.com/platewashing to learn more.

21

Figure 14. Assay precision. Red and gray bars each represent 24 replicates measured on separate days. CVs in all cases were <10%. Data were generated using Invitrogen™ Cytokine Human Magnetic 10-Plex Panel (Cat. No. LHC0001M).

Figure 13. Correlation of Luminex™ multiplex panel with ELISAs. Cerebrospinal fl uid was analyzed with both the Invitrogen™ Human Aβ/Tau Neurodegenerative Magnetic 3-Plex Panel Kit (Cat. No. LHN0001M) and 3 different ELISA kits (Cat. No. KHB3481, KHB3441, and KHB0041). The R2 values shown indicate a strong correlation between the sample concentration values obtained using the ELISA and Luminex quantitative methods.

Table 4. Specifi cations for kit development.

Specifi cations Description

Benchmarking to ELISA Correlates with ELISA data

Recovery Tested on serum and plasma

Sensitivity* Physiologically relevant levels, <10 pg/mL (based on detectable signal >2 SD above background)

Precision Inter-assay CV: (between assays) <10%Intra-assay CV: (within assays) <10%

Specifi city Cross-reactivity tests are performed with other analytes and antibodies

Linearity of dilution High coeffi cient of correlation between sample dilutions and expected concentration over the range of the assay

Parallelism to natural samples Recombinant standards are compared to natural samples to ensure equivalency * Every kit has its own specifi cations. Please consult the protocol insert in your specifi c Invitrogen™ multiplex kit.

Med

ian

fluor

esce

nce

inte

nsity

1,400

1,200

1,000

800

600

400

200

0IL-1� GM-CSF IL-5IL-6IL-10 IFN-γ TNF-α IL-2 IL-4 IL-8

Rigorous assay validation helps ensure consistent, reliable results

Our multiplex kits undergo rigorous quality testing (Table 4) and are correlated to our ELISA kits if available (Figure 13) to offer confi dence that switching between protein analysis platforms will provide comparable analytical results.

Luminex 3-plex (pg/mL) Luminex 3-plex (pg/mL)Luminex 3-plex (pg/mL)

6,000

5,000

4,000

3,000

2,000

1,000

0

5,000

4,000

3,000

2,000

1,000

00 500 1,000 1,500 2,000 0 100 200 300 400 500 600 700 800 0 200 400 600 800 1,000 1,200 1,400 1,600

R2=0.8643R2=0.8917

Aβ42Tau Aβ40

R2=0.9471

ELI

SA

(pg/

mL)

ELI

SA

(pg/

mL)

ELI

SA

(pg/

mL)

350

300

250

200

150

100

50

0


6,000

5,000

4,000

3,000

2,000

1,000

0

5,000

4,000

3,000

2,000

1,000

00 500 1,000 1,500 2,000 0 100 200 300 400 500 600 700 800 0 200 400 600 800 1,000 1,200 1,400 1,600

R2=0.8643R2=0.8917

Aβ42Tau Aβ40

R2=0.9471

ELI

SA

(pg/

mL)

ELI

SA

(pg/

mL)

ELI

SA

(pg/

mL)

350

300

250

200

150

100

50

0


6,000

5,000

4,000

3,000

2,000

1,000

0

5,000

4,000

3,000

2,000

1,000

00 500 1,000 1,500 2,000 0 100 200 300 400 500 600 700 800 0 200 400 600 800 1,000 1,200 1,400 1,600

R2=0.8643R2=0.8917

Aβ42Tau Aβ40

R2=0.9471

ELI

SA

(pg/

mL)

ELI

SA

(pg/

mL)

ELI

SA

(pg/

mL)

350

300

250

200

150

100

50

0

22

Luminex instruments for xMAP technologyWith over 10,000 instruments placed globally, the Luminex platform is well recognized as the preferred multiplexing platform. All of our multiplex assay kits can be run on any of these Luminex™ systems—MAGPIX, 100/200, Bio-Plex, or FLEXMAP 3D™ systems. For questions regarding the instruments, email LuminexFAS@thermofi sher.com.

Figure 15. Recovery. To evaluate magnetic multiplex assays, samples of 23 human protein markers were spiked into a sample of human serum, and the sample was processed using the manufacturer’s instructions. The multiplex sample was quantifi ed on the MAGPIX system, and percent recovery calculated for (A) the individual markers and (B) as an average for the entire group.

A B100%

75%

50%

25%

0%

FGF-

basic

IL-10

IL-13 IL-

6IL-

12

Eotax

inIL-

17

MIP-1α

GM-CSF

MCP-1

MIP-1β

G-CSF

IL-1β

VEGFIL-

15IFN

-γ

IL-1R

A

TNF-α

IL-2

IL-7IP

-10

IL-4

IL-8

Invitrogen assay Supplier 2 Supplier 3

Rec

over

y

100%

75%

50%

25%

0%Invitrogen assay

79%

Supplier 3

17%

Supplier 2

66%

Rec

over

y

Recovery test comparison

MAGPIX 100/200 FLEXMAP 3D

Over 1,000 references—visit our kit product pages or contact our Technical Support team at [email protected] for informative and valuable citations.

23

Watch video about tips and tricks on using assays for the Luminex platform.Go to thermofisher.com/customluminex to learn more.

24

Multiplex assay kits for the Luminex platform Multiplex assays are performed in much the same way as ELISAs with the exception that antibody-specific capture beads are added to wells of a 96-well plate, instead of capturing antibodies attached to the wells (Figure 16). Samples are then placed into the microplate wells. Invitrogen™ multiplex assay kits are provided with post-lyophilized calibrated protein standards for quantitation and are calibrated to NIBSC, if available.

After incubation, the beads are washed either through filtration (for polystyrene beads) or a handheld magnet/plate washer (for magnetic beads). The beads are resuspended in the secondary detection antibody solution. Another incubation and washing step is performed followed by the addition of streptavidin-RPE. The beads are then washed again and are ready to be analyzed on a Luminex instrument.

Figure 16. Invitrogen™ extracellular multiplex magnetic assay protocol.

Wash twice

Shake at RT in the dark


Wash twice

Serum, plasma, or tissue culture supernatantStandard Sample type:

Add 1X antibody beads

Add standard and blank/background

Add assay diluent

Wash twice

Add 1X biotinylated detector antibody

Read in Luminex xMAP system

Total time: 3.5 hr

Shake

Add sample

Add 1X SAV-RPE

Add wash solution


Wash three times

Protein Bead Detector antibody

R-phycoerythrin(RPE)

Decant liquid

Decant liquid

Decant liquid

Incubate on magnet and decant liquid

Add incubation buffer Add incubation buffer

25

NEW For your ordering convenience we will soon offer more magnetic singleplexes as catalog items.

Each singleplex kit typically includes: • Antibody-coated capture beads


• Standard

Buffer kit sold separately and typically includes:• Diluents

• Buffers

• SAV-RPE concentrate

• Wash solution

• Magnetic/fl at-bottom plate

• Filter plate

• Protocol

Marker Cat. No.

IL-8 LHC0081M

IL-9 LHC0091M

IL-10 LHC0101M

IL-12 (p40/p70) LHC0121M

IL-12 (p70) LHC9121M

IL-13 LHC0131M

IL-15 LHC0151M

IL-16 LHC0161M

IL-17A LHC0171M

IL-17F LHC4021M

Insulin LHP0021M

IP-10 LHC1081M

Leptin LHP0011M

Lipocalin-2 LHC0111M

MCP-1 LHC1011M

MCP-2 LHC1111M

MCP-3 LHC1571M

MDC/CCL22 LHC0251M

MIG LHC1091M

MIP-1α LHC1021M

MIP-1β LHC1051M

MIP-3α LHC0261M

P-Selectin LHC0201M

PAI-1 LHC0221M

PDGF-BB LHG0041M

PECAM LHC0211M

RANTES LHC1031M

Resistin LHP0051M

TARC LHC0271M

Marker Cat. No.

HumanAdiponectin LHP0041M

BAFF LHC0341M

CD30 (soluble) LHS6031M

DR5 LHR0051M

E-selectin LHC0191M

EGF LHG0061M

ENA-78 LHC0231M

Eotaxin LHC2231M

FGFb LHG0021M

Fibrinogen* LHP0091M

G-CSF LHC2031M

GM-CSF LHC2011M

GROα LHC1061M

HGF LHG0071M

I309/CCL1 LHC0241M

ICAM LHC0141M

IFN-α LHC4011M

IFN-γ LHC4031M

IL-1α LHC0811M

IL-1RA LHC0711M

IL-2 LHC0021M

IL-2R LHR0021M

IL-3 LHC0031M

IL-4 LHC0041M

IL-5 LHC0051M

IL-6 LHC0061M

IL-6R (soluble) LHR0061M

IL-7 LHC0071M

Magnetic singleplex kits

* Not recommended to be multiplexed with other targets. Kit includes its own buffer kit.

26

Marker Cat. No.

UltrasensitiveGM-CSF LHC2013M

IFN-γ LHC4033M

IL-1β LHC0013M

IL-2 LHC0023M

IL-4 LHC0043M

IL-5 LHC0053M

IL-6 LHC0063M

IL-8 LHC0083M

IL-10 LHC0103M

TNF-α LHC3013M

Ultrasensitive Buffer Kit** LHB0003M

Marker Cat. No.

Human (continued)

TGF-β1* (Multispecies) LHG0121M

TNF-RI LHC3021M

TNF-RII LHC3031M

TNF-α LHC3011M

VCAM LHC0181M

VEGF LHG0111M

MonkeyEGF LPC0031M

Eotaxin LPC2231M

FGFb LPC0091M

G-CSF LPC2031M

GM-CSF LPC2013M

IFN-γ LPC4031M

IL-1RA LPC0711M

IL-1β LPC0011M

IL-2 LPC0021M

IL-4 LPC0041M

IL-5 LPC0051M

IL-6 LPC0061M

IL-7 LPC0171M

IL-8 LPC0081M

IL-10 LPC0101M

IL-12 LPC0121M

IL-15 LPC0151M

IP-10 LPC1171M

MCP-1 LPC1011M

MIG LPC0140M

MIP-1α LPC1021M

MIP-1β LPC1051M

RANTES LPC1031M

TNF-α LPC3011M

VEGF LPC0181M

Magnetic singleplex kits (continued)

Magnetic buffer kit

Description Cat. No.

Extracellular Protein Buffer Magnetic Reagent Kit LHB0001M

* Not recommended to be multiplexed with other targets. Kit includes its own buffer kit.** Need to purchase 1 buffer kit to mix and match any ultrasensitive markers.

27

Marker Cat. No.

Leptin LHP0011

MCP-1 (CCL2) LHC1011



MIP-1α (CCL3) LHC1021

MIP-1β (CCL4) LHC1051

PDGF-BB LHG0041

RANTES (CCL5) LHC1031

SAA (Serum Amyloid A) LHP0061

Tau (total) LHB0041

TGF-β1 (activated)* LHG0121

TNF-α LHC3011

TNF-α (ultrasensitive) LHC3013

TNF-RI (soluble) LHC3021

TNF-RII (soluble) LHC3031

VEGF LHG0111

Human, mouse

Akt (total) LHO0091

Akt [pS473] LHO0101

GSK-3β (total) LHO0451

GSK-3β [pS9] LHO0461

IGF-1R (total) LHO0491

IR (total) LHR9111

IGF-1R [pY1135/pY1136] LHO0501

IR [pY1162/pY1163] LHR9131

p70-S6K (total) LHO0181

p70-S6K [pT421/pS424] LHO0191

PRAS40 (total) LHO0411

PRAS40 [pT246] LHO0421

Monkey

G-CSF LPC2031

IFN-γ LPC4031

IL-2 LPC0021

IL-4 LPC0041

IL-6 LPC0061

IL-8 LPC0081

IL-10 LPC0101

IL-17 LPC0171

IP-10 (magnetic) LPC1171M

MCP-1 (CCL2) LPC1011

MIP-1α (CCL3) LPC1021

MIP-1β (CCL4) LPC1051

RANTES (CCL5) LPC1031

TNF-α LPC3011

Marker Cat. No.

Human

Adiponectin LHP0041

Aβ40 LHB3481

Aβ42 LHB3441

BDNF LHC7071

DR5 LHR0051

EGF LHG0061

EGFR (total) LHR9061

EGFR [pY1068] LHR9081

Eotaxin (CCL11) LHC2231

FGF-basic LHG0021

G-CSF LHC2031

GDNF LHC7041

GM-CSF LHC2011

GRO-α LHC1061

HGF LHG0071

IFN-α LHC4011

IFN-γ LHC4031

IGF-1R [pY1135/pY1136] LHO0501

IL-1α LHC0811

IL-1β LHC0011

IL-1β (ultrasensitive) LHC0013

IL-1RA LHC0711

IL-2 LHC0021

IL-2R LHR0021

IL-3 LHC0031

IL-4 LHC0041

IL-5 LHC0051

IL-6 LHC0061

IL-6 (ultrasensitive) LHR0063

IL-6R (soluble) LHR0061

IL-7 LHC0071

IL-8 LHC0081

IL-8 (ultrasensitive) LHC0083

IL-10 LHC0101

IL-12 (p40/p70) LHC0121

IL-12 (p70) LHC9121

IL-13 LHC0131

IL-15 LHC0151

IL-16 LHC0161

IL-17 LHC0171

Insulin LHP0021

IP-10 LHC1081

IRS-1 (total) LHO0511

Nonmagnetic (polystyrene) singleplex kits

Have technical questions? Need help getting started? Email [email protected] to get a one-on-one technical consultation.

28

Marker Cat. No.

Mouse

FGF-basic LMG0021

G-CSF LMC2031

GM-CSF LMC2011

IFN-γ LMC4031

IL-1α LMC0811

IL-1β LMC0011

IL-2 LMC0021

IL-4 LMC0041

IL-5 LMC0051

IL-6 LMC0061

IL-10 LMC0101

IL-12 (p40/p70) LMC0121

IL-13 LMC0131

IL-17 LMC0171

KC LMC1061

MCP-1 (CCL2) LMC1011

MIG (CXCL9) LMC1091

MIP-1α (CCL3) LMC1021

MIP-1β (CCL4) LMC1051

RANTES (CCL5) LMC1031

TNF-α LMC3011

VEGF LMG0111

Rat

GM-CSF LRC2011

IFN-γ LRC4031

IL-1α LRC0811

IL-1β LRC0011

IL-2 LRC0021

IL-4 LRC0041

IL-6 LRC0061

IL-10 LRC0101

IL-12 (p40/p70) LRC0121

TNF-α LRC3011

Description Species Cat. No.

Human Extracellular Protein Buffer Reagent Kit

Human, monkey LHB0001

Intracellular Protein Buffer Reagent Kit

Human, mouse, rat LHB0002

Human Ultrasensitive Cytokine Buffer Reagent Kit

Human LHB0003

Mouse or Rat Extracellular Protein Buffer Reagent Kit

Mouse, rat LMB0001

Multispecies Growth Factor Buffer Reagent Kit

Human, monkey, mouse, rat

LMB0002

Neuroscience Buffer Reagent Kit Human LNB0001

Typically includes diluents, buffers, SAV-RPE concentrate, wash solution, filter plate, and protocol.

“I had an excellent service experience

with the field application specialist.

I found him very personable and

knowledgeable. I thought the

excellent service and resolution of

my issues in this instance warranted

special recognition.”

Thomas Chu, MD Clinical Fellow

University of British Columbia

Nonmagnetic (polystyrene) singleplex kits (continued) Nonmagnetic (polystyrene) buffer kits

29

Premixed multiplex panels

DescriptionMarker (Buffer kit included in each panel)

Cat. No.


Human

New Human Cytokine 35-Plex Panel

EGF, eotaxin, FGF-basic, G-CSF, GM-CSF, HGF, IFN-α, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-2R, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40/p70), IL-13, IL-15, IL-17, IL-17F, IL-22, IP-10, MCP-1, MIG, MIP-1α, MIP-1β, RANTES, TNF-α, VEGF

LHC6005M

Human Cytokine 30-Plex Panel

EGF, eotaxin, FGF-basic, G-CSF, GM-CSF, HGF, IFN-α, IFN-γ, IL-1α, IL-1RA, IL-2, IL-2R, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40/p70), IL-13, IL-15, IL-17, IP-10, MCP-1, MIG, MIP-1α, MIP-1β, RANTES, TNF-α, VEGF

LHC6003 LHC6003M

Human Cytokine 25-Plex PanelEotaxin, GM-CSF, IFN-α, IFN-γ, IL-1β, IL-1RA, IL-2, IL-2R, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40/p70), IL-13, IL-15, IL-17, IP-10, MCP-1, MIG, MIP-1α, MIP-1β, RANTES, TNF-α

LHC0009 LHC0009M

Human Cytokine 10-Plex Panel GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, TNF-α LHC0001 LHC0001M

Human Ultrasensitive Cytokine 10-Plex Panel

GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, TNF-α LHC6004 LHC6004M

New Human Adipokine 14-Plex PanelIL-1β, IL-6, IL-8, IL-10, MCP-1, leptin, SAA, HGF, insulin, lipocalin-2, TNF-α, BAFF, resistin, PAI-1

LHC0017M

Human Apolipoprotein 5-Plex Panel ApoA1, ApoB, ApoE, adiponectin, CRP LHP0001M

Human Adhesion 6-Plex Magnetic Panel ICAM-1, VCAM-1, E-selectin, P-selectin, PECAM-1, PAI-1 LHC0016M

New Human Aβ/Tau Neurodegenerative 3-Plex Panel

Aβ40, Aβ42, tau (total) LHN0001M

Human Apoptosis 3-Plex Panel Cytochrome c, caspase-3 [175/176], PARP [214/215] LHO0007

Human Chemokine 5-Plex Panel Eotaxin, MCP-1, MIP-1α, MIP-1β, RANTES LHC0005

Human Chemokine 10-Plex PanelEotaxin, GRO-α, IP-10, MCP-1, MCP-2, MCP-3, MIG, MIP-1α, MIP-1β, RANTES

LHC6001

Human Chemokine II 5-Plex PanelENA-78 (CXCL5), I-309 (CCL1), MDC (CCL22), MIP-3α (CCL20), TARC (CCL17)

LHC0012

Human Cytokine II 5-Plex Panel IFN-α, IL-12 (p40/p70), IL-13, IL-15, IL-17 LHC0007

Human Death Receptor 3-Plex Panel TNF-RI, TNF-RII, DR5 LHC0006

Human Growth Factor 4-Plex Panel EGF, FGF-basic, G-CSF, VEGF LHC0004

Human Inflammatory Cytokine 5-Plex Panel

GM-CSF, IL-1β, IL-6, IL-8, TNF-α LHC0003 LHC0003M

Human Th1/Th2 Cytokine 5-Plex Panel IFN-γ, IL-2, IL-4, IL-5, IL-10 LHC0002

Human Th1/Th2/Th17 8-Plex Panel IFN-γ, IL-2, IL-4, IL-5, IL-9, IL-10, IL-13, IL-17 LHC0015M

Human Acute Phase 4-Plex Panel β2 microglobulin, haptoglobin, CRP, Gc globulin LHC6006

Each premixed panel kit typically includes: • Premixed antibody–

coated capture beads• Premixed detector antibody• Standard• SAV-RPE concentrate

Watch how to use the handheld magnet for magnetic assays. Go to thermofisher.com/platewashing to learn more.

• Diluent buffers• Wash solution• Filter plate• Reagents sufficient

for 96 tests

Have technical questions? Need help getting started? Email [email protected] to get a one-on-one technical consultation.

30

Accessories for Luminex instruments


xMAP Sheath Fluid (1X), 20 L 4050000

xMAP Sheath Fluid (20X),1 L 4075680

xMAP Classification Calibrator Microspheres (CAL1)* L100CAL1

xMAP Reporter Calibrator Microspheres (CAL2)* L100CAL2

xMAP Classification Control Microspheres (CON1)* L100CON1

xMAP Reporter Control Microspheres (CON2)* L100CON2

MAGPIX Performance Verification Kit (25 reactions) MPXPVERK25

MAGPIX Calibration Kit (25 reactions) MPXCALK25

MAGPIX Drive Fluid (4 pack) MPXDF4PK

FLEXMAP 3D Performance Verification Kit (25 ractions) F3DPVERK25

FLEXMAP 3D Calibration Kit (25 reactions) F3DCALK25

* 5 mL

Premixed multiplex panels

DescriptionMarker (Buffer kit included in each panel)

Cat. No.


Human, mouse, rat

Akt Phospho 7-Plex PanelAkt [pS473], GSK-3β [pS9], IRS-1 [pS312], IGF-1R [pY1135/pY1136], IR [pY1162/pY1163], p70-S6K [pT421/pS424], PRAS40 [pT246]

LHO0001 LHO0001M

Akt Total 7-Plex Panel Akt, GSK-3β, IGF-1R, IR, IRS-1, p70-S6K, PRAS40 LHO0002 LHO0002M

Monkey

Monkey Cytokine 29-Plex Panel

EGF, eotaxin, FGF-basic, G-CSF, GM-CSF, HGF, IFN-γ, IL-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-15, IL-17, IP-10, I-TAC, MCP-1, MDC, MIF, MIG, MIP-1α, MIP-1β, RANTES, TNF-α, VEGF

LPC0005M

Monkey Chemokine 5-Plex Panel IL-8, MCP-1, MIP-1α, MIP-1β, RANTES LPC0002

Monkey Cytokine 5-Plex Panel IFN-γ, IL-2, IL-4, IL-10, TNF-α LPC0001

Mouse

Mouse Cytokine 20-Plex PanelFGF-basic, GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 (p40/p70), IL-13, IL-17, IP-10, KC, MCP-1, MIG, MIP-1α, TNF-α, VEGF

LMC0006 LMC0006M

Mouse Cytokine 10-Plex PanelGM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 (p40/p70), TNF-α

LMC0001 LMC0001M

Mouse Inflammatory Cytokine 4-Plex Panel

GM-CSF, IL-1β, IL-6, TNF-α LMC0003 LMC0003M

Mouse Th1/Th2 6-Plex Panel IFN-γ, IL-2, IL-4, IL-5, IL-10, IL-12 (p40/p70)

Rat

Rat Cytokine 10-Plex Panel GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12 (p40/p70), TNF-α

LRC0002M

Swine

Swine Cytokine 7-Plex Panel IFN-α, IFN-γ, IL-1β, IL-4, IL-8, IL-10, TNF-α LSC0001M

Accessories


Magnetic 96-Well Separator A14179

96-Well Filter Plate LCP0000

Wash Buffer (20X), 15 mL WB04

(continued)

Request a custom blendedmultiplex assay

Made-to-order multiplex assay system: Design your custom Invitrogen multiplex assay and request a quote using our Web-based design tool.

Each kit typically includes: • Premixed antibody-coated capture beads

• Premixed detector antibody

• Standard

• SAV-RPE concentrate

• Diluent buffers

• Wash buffer

• Plate

We now have a web-based design tool for your made-to-order assays. To design your own custom assay blend, go to thermofi sher.com/customluminex

31

Our custom-blend option allows you to select individual protein targets from our menu. We will mix a custom-blended multiplex assay for you and optimize to help ensure low background, high signal-to-noise ratios, and linearity of dilution. Your reagents will arrive in a ready-to-use format that will help you avoid mixing errors and guessing dilutions when you blend the assay yourself.

Meet your match with Invitrogen antibodies

Study thousands of human proteins at onceWe offer the ProtoArray™ Human Protein Microarray, which contains over 9,000 unique human proteins selected from multiple gene families. Perform a variety of assays including biomarker identification, drug target discovery, enzyme substrate identification, antibody specificity profiling, and protein–protein interaction studies.

Learn more at thermofisher.com/protoarray

Measuring autoantigens using the Luminex platformWe offer the ProtoPlex™ Autoimmune Panel to screen 19 disease-linked autoantigens with as little as 2.5 µL of serum.

Learn more at thermofisher.com/protoplex

We offer over 42,000 primary, secondary, and custom antibodies covering over 70% of the proteome and over 50 research areas. All of our antibodies are validated and guaranteed to perform in the stated application and species.

Easily find your match using our antibody search tool at thermofisher.com/antibodies

* Terms and conditions apply. For complete details, go to thermofisher.com/antibody-performance-guarantee

Find out more at thermofisher.com/immunoassaysFor Research Use Only. Not for use in diagnostic procedures. © 2016 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Luminex is a trademark of Luminex Corporation. Luminex 100/200, FLEXMAP 3D, Luminex, MAGPIX, xMAP, and xPONENT are trademarks of Luminex Corporation. Bio-Plex is a trademark of Bio-Rad Laboratories, Inc. COL31172 0516

Performanceguaranteed*

elisa and and and multiplexmultiplex assays assays...elisa kits invitrogen elisa kits allow specifi...

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