Electrochemical Detector

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Autosampler

Complete HPLC-ECD

ECD-700

Eicom ECD-700 Electrochemical Detector for HPLC

Eicom ECD-700

Features

Eicom's electrochemical detector, the ECD-700, offers superior stability and sensitivity that reviles that of mass spectrometry detectors, but without the cost and special expertise. Electrochemical detectors easily detect a wide range of easily oxidized or reduced compounds, and you can rely of Eicom's extensive experience to help youfind the best set-up for your research goals. Depending of the requirements of a particular method, the ECD-700 can be configured with up to two detector cells. Both amperometric and coulometric types are available.

In amperometry the analyte contacts the surface of a solid working electrode. Amperometry achieves better signal-to-noise ratios resulting in better sensitivity. Coulometry depends on porous working electrode to promote the complete electrolysis of the analyte as it passes. At Eicom, we find the best approach is to employ coulometric cells to prepare the analyte for detection with an amperometric cell.

• High Sensitivity, 30 fg sensitivity for Dopamine at S/N ratio=3.• Easy system setup and “ready-to-use” applications.• Instant access to the working electrode of the amperometric detector cell.• Expandable design for more demanding users or protocols.

ConfigurationsStandardOne amperometric detector cell with single graphite working electrode.

A: Amperometric detector, dA: Dual amperometric working electrodes that share a common reference electrode and can be installed in parallel or series with the flow. C: Coulometric flow cell.

Optional Configurations

Standard

Option 1

Option 2

1st Detector CellA

dAA /dA

Option 3 A /dA/CAC

2nd Detector Cell

Coulometric Cell

Amperometric Cell

Amperometry Dual Amperometry Coulometry

or

Working Electrode Designs

Amperometry vs. Coulometry

Simplicity + ExpandabilityThe keyword during the development of the ECD-700 was “expandability”. Over the past 25 years of Eicom ECD products, our goal has been to help users “kick start” new projects with our “ready-to-use” applications and reliable instruments. And now for more demanding applications, we have introduced the ECD-700, which has two detector cell capacity and allows for many combinations of detector types and working electrodes.

Variety of Working Electrodes Materials

• Graphite Electrode WE-3G, very stable and best S/N ratio. Little maintenance is required. Included in the standard configuration.

• Pure Graphite WE-PG, stable even at higher applied voltage

• Glassy Carbon WE-GC, conventional working electrode

• Platinum WE-PT, good for hydrogen peroxide. Commonly used with inline enzyme reactor columns.

• Gold WE-AU, well suited for thiol group detection

• Silver WE-AG

Dual working electrode for use with Eicom's amperometric detector cell.

• Graphite WED-3G

• Glassy Carbon WED-GC

Coulometric detector cell working electrode material.

• Porous Carbon (refer to Option 3 on previous page)

Heart of Sensitivity, Amperometric Detector Cell

Diagram of Detector Cell

Eicom's advanced working electrode design requires much less maintenance than many ECD users may have become accustomed to. When the need does arise, the electrode can easily be accessed to replace it or wipe off any chemical build-up. Even after the cell is opened, the initial baseline signal is quickly restored without worry.

�e pressure inside the cell is maintained by a spring-loaded ball assembly that ensures a constant pressure. �e ball compresses the working electrode assembly at one point to evenly distribute the pressure across the working electrode.

EICOM

WE-XX

GS-XX

Working Electrode

Gasket

Eicom ECD-700

Many “Ready-to-use” applicationsEicom provides complete, step-by-step protocols for a variety of applications. These protocols include instructions for sample collection and preparation, mobile phase recipes, storage and preparation of standards, and exact instrument settings. If you want to modify a method or create your own method, we can advise you on where to start and give you feedback about the data along the way.

Pulse DamperElectrochemical detection is extremely sensitive to changes in pressure. Consequently, each stroke of an HPLC pump can cause oscillations in the baseline signal, which necessitates the use of a pulse damper to obtain the lowest detection limit. If you will not be using an Eicom pump, we recommend using a pulse damper, such as Eicom PL-500. A Pulse damper is not required for Eicom pumps.

Specifications of ECD-700 Standard Configuration

Typical EC detectable compounds

US Office7313 Carroll Road Suite F,San Diego, CA 92121Phone +1-888-680-7775 www.eicom-usa.com

Eicom Europe1A Dartmouth TerraceRanelagh Dublin 6, IrelandPhone +353-1-902-2700 www.eicomeurope.com

Headquarters113 Kitaenmendencho Shimotoba FushimikuKyoto-Shi Kyoto-Fu JapanPhone +81-75-622-2112www.eicom.co.jp

Columns

Detection Method

Working Electrode

Applied Potential Range

Auto Zero Function

Reference Electrode

Counter Electrode

Cell Volume

Inlet Tubing OD

Input

Output

Auto Zero Input

Log File

Amperometric, DC with 3 electrode potentiostat

Standard single graphite. Optional dual working electrode with a shared reference electrode.

-2,000 mV ~ +2,000 mV in increments of 1mV

-1,000 nA ~ +1,000 nA, resets in less than 0.7 sec. Each detector cell zeros separately.When dual electrode is installed, the signal from both applied voltages zero together.

Ag / AgCl

Stainless Steel 316

Active area = 1 µL. Outlet has 100 µL reservoir

1/16 inch

Contact closure signal, DC 0 V ~ 5 V, ON ≥ 1 mV OFF ≤ 10 µA

Delay of 0 sec, 3 sec and 6 sec after 1sec signal input

Accept DC 0 V ~ 5 V, ON ≥ 1 mV OFF ≤ 10 µA

User can save a log of reference and working electrode replacements for each channelthrough keypad

Phenols

Catechols

Indoles

Anilines

Quinones

Tocopherols

Estrogens

Hydroxycoumarins

Purines

Opiates

Phenothiazines

Thiols

Specification and design of products may change without notice.

Eicom ECD-700

Complete HPLC-ECD

Eicom 700 Series Complete HPLC-Electrochemical Detector System

Sensitivity comes from more than just the detector.

If you need the very best performance of Eicom ECD-700, we recommend using the three 700 Series components as one system. The 700 Series includes an HPLC Pump (EP-700), Temperature Controller (ATC-700) and Electrochemical Detector (ECD-700). When used together, we promise the best results.

To achieve optimal performance from electrochemical detectors, there are important considerations. The Eicom 700 Series Total System addresses all of these requirements to ensure the best sensitivity.

• A chemically inert or, at a minimum, passivated interior because metal ions increase the background signal • A means to stabilize the baseline because oscillations in the pressure caused by the HPLC pump produce concomitant oscillations in the signal baseline • Effective temperature control because electrochemical detection is very sensitive to temperature

Eicom EP-700 HPLC PumpNo more pulse dampers required! Revolutionary pump design can effectively quench flow pulses.

Built-in, computer control continually adjusts the piston velocity during each stroke to minimize fluctuations in the pressure. Pulse free pumping works in both gradient and isocratic modes. Because

Standard Configuration

EP-700M --- one micro flow pump (10 to 500 µL /min )

Options

EP-700H --- one high flow type pump

EP-700MM --- two micro flow pumps with gradient controller

EP-700HH --- two high flow pumps with gradient controller

EP-700MH --- one micro flow and one high flow pump

Eicom Complete 700 Series

the EP-700 does not require a damper, the volume between a column and the HPLC pump is minimal. This means rapid acquisition of steady-state pressure after starting the pump and more immediate solvent effects that are important to accurate gradients. An innovative 300 µL volume degasser is built in to guarantee continuous pumping and preventing noise at the detector.

Pulse-free ModeOFF

Pulse-free ModeON

Signal Baselines

Eicom ATC-700 Temperature Controller

Eicom AS-700 INSIGHT Autosampler

The Eicom ATC-700 uses a Peltier element that both heats and cools by convection to maintain precise temperature control. The system responds to just a 0.05°C change and controls the temperature of the both the columns and ECD cells.

We know your time is limited. Eicom's reliable autosampler injects samples while you are away. Autosamplers are must have item for knowledgeable HPLC users. For more details, please see the INSIGHT Autosampler brochure.

INSIGHT

SpecificationsSetting Range

Recommended Range

Dimension

Weight

Power Supply

15 to 55°C

Recommended Range; 20 to 45°C at 25°C ambient

400 (w) x 400 (d) x 145 (h) cm

about 10 kg

AC 100-240 V, 50/60 Hz, 210 VA

Type

Construction

Flow Rate Ranges

Liquid Contacting Surfaces

Pressure Limit

Gradient Mode

External Signal

Degassing Chamber Volume

Lines

Internal Volume

Maximum Flow Rate

Dimension

Weight

Power Supply

Temperature Regulation

Setting Range

Recommended Range

Column Capacity

Dimension

Weight

Power Supply

Pump Unit (EP-700)

Casing (EP-700)

Temperature Controller (ATC-700)

Degasser Unit (EP-700)

Specifications of EP-700 and ATC-700

Dual reciprocating piston pump

Chemically inert materials at all liquid contacting surfaces

Micro flow type:100 to 500 µL/min with 1 µL/min increments, 4 µL/stroke

High flow type:0.5 to 3.00 mL/min with 0.01 mL/min increments, 32 µL/stroke

PEEK, Sapphire, Ruby, PTFE, PCTFE

20 MPa

High pressure linear gradient, 10 steps.Available when 2 same pump installed.

Input: Gradeint Start/Stop, Gradient Initialization Output: Pump Pressure

300 µL x 2 channels

2 Channels

300 µL/channel

3 mL/min

400 (w) x 400 (d) x 190 (h) mm

approx. 13 kg with one pump, approx. 16 kg with two pumps

AC 100-240 V, 50/60 Hz, 200 W

Peltier device for heating and cooling by convection

15 to 55°C, 1°C increament

20 to 45°C at 25°C ambient

25 cm x 2 HPLC columns

400 (w) x 400 (d) x 145 (h) mm

approx. 10 kg

AC 100-240 V, 50/60 Hz, 210 VA

US Office7313 Carroll Road Suite F,San Diego, CA 92121Phone +1-888-680-7775 www.eicom-usa.com

Eicom Europe1A Dartmouth TerraceRanelagh Dublin 6, IrelandPhone +353-1-902-2700 www.eicomeurope.com

Headquarters113 Kitaenmendencho Shimotoba FushimikuKyoto-Shi Kyoto-Fu JapanPhone +81-75-622-2112www.eicom.co.jp

Eicom Complete 700 Series

Specification and design of products may change without notice.