effects of high temperature and explosions on forensic dna€¦ · marwan el khoury supervised by:...

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Marwan El Khoury Supervised by : Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature and Explosions on Forensic DNA intrepid-forensics.eu/project-3/ [email protected]

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Page 1: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature

Marwan El Khoury

S u p e r v i s e d b y : J o n W e t t o n , D r .Jobling Mark, Prof.Hillman Rob, Prof.

Effects of High Temperature and Explosions on Forensic DNA

intrepid-forensics.eu/project-3/ [email protected]

Page 2: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature

Copyright © CNT Group; 2000

• Crime and Incidents involving Fire, Explosions, and other sources of high temperature.

• Post-PhD ambition to study Crime, Security and Safety globally, across disciplines, with a focus on the MENA region.

Page 3: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature

Accidental Fire

71 deaths

Challenging identification …

Grenfell Tower – London, June 14 2017

Page 4: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature

• Testing the effects of fire on DNA.

• Interdisciplinary training with Academics, Police and Industry partners.

Page 5: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature

140 ℃ 180 ℃ 200 ℃4 ℃ 140 ℃ 180 ℃ 200 ℃4 ℃

140 ℃ 180 ℃4 ℃

High Temperatures (140,180, 200 ℃)

Page 6: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature

180 ℃

High Temperature on 23 YSTRs-CE

4 ℃ 140 ℃

180 ℃ 200 ℃

Page 7: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature

High Temperature on Auto/Y/SNPs-MPS

4 ℃ 180 ℃

200 ℃

Page 8: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature

Terrorism

3000 deaths

DNA ID success: 65%

World Trade Center – New York, September 11 2001

Page 9: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature

19 October 2012, Beirut – Lebanon (bbc.co.uk/news/in-pictures-20008421).

Explosions … Followed by Fire

Page 10: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature

National Consortium for the Study of Terrorism and Responses to Terrorism (START). (2017). Global Terrorism Database. Retrieved from https://www.start.umd.edu/gtd

Terrorism - Predominant Use of Explosives

Page 11: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature

• Testing the effects of explosions on DNA.

• Collaboration with US Law Enforcement (FBI, ATF and local Police Bomb squads) and Academics (Penn State University).

Page 12: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature
Page 13: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature
Page 14: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature

Post-Blasts Items Collection

Page 15: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature

DBSP explosions (Auto & Y – CE)

C4 explosions (Auto & Y – CE)

Page 16: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature

C4 Flame duration ≈ 40 milliseconds.

Page 17: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature

DBSP Flame duration ≈ 850 milliseconds.

Page 18: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature

Summary of Works Completed• PhD results:

• 140℃: full profile, good quality.• 180℃ : partial profile, degraded DNA.• 200℃ : null result.• C4 explosion (outside): full profile, good

quality.• C4 explosion (inside): no recovery.• DBSP (outside and inside): 61.5% partial

profile, degraded DNA.

• Forensics (and Criminology) training programme.• Collaboration with US Law Enforcement and

Academics.

Page 19: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature

MENA PROJECT • #scienceforjustice #scienceforsecurity.

• Studying, Solving and Preventing Crimes, Disasters and other Threats.

• Best use of Resources and Technology.

• Collaborating with Fellow Law Enforcement and Academic Institutions.

• Building and Maintaining the citizen’s Safety, Security, and sense of Justice.

Page 20: Effects of High Temperature and Explosions on Forensic DNA€¦ · Marwan El Khoury Supervised by: Jon Wetton, Dr. Jobling Mark, Prof. Hillman Rob, Prof. Effects of High Temperature