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Effecten van fructooligosacchariden
bij zogende biggen
Dirkjan Schokker et al. ~ 21-4-2015
Animal Breeding & Genomics Centre
Acknowledgements
Animal Breeding & Genomics Centre
Ministerie van Economische Zaken
Productschap Diervoeder (PDV)
Productschap Pluimvee en Eieren (PPE)
Productschap Vee en Vlees (PVV)
Agrifirm Group
ForFarmers Hendrix
De Heus
Nutreco
VION Food Group
MSD - Animal Health
VanDrie Group
Denkavit
Voeding, Darmgezondheid, en
Immuniteit
Focus on gut development in early life
Animal Breeding & Genomics Centre
Morphological
developmentImmune system
programming
Microbiota
colonization
Functional
differentiation
Nutritional
factorsManagement
factors
Objective
Investigate the effect of FOS on microbiota colonization and immune
development of the intestine in relation to immune competence
in suckling piglets
Animal Breeding & Genomics Centre
Study design
Control
FOS
Day 2 14 25
8 8 8
8 8
Animal Breeding & Genomics Centre
� 2 * 5gr FOS oral gavage
� From day 2~6 diluted in 15ml water
� Due to vomiting/diarrhoea day 7~14 diluted in 6ml water
Performance data
0
1
2
3
4
5
6
7
8
9
10
0 3 7 14 21 27
Weig
ht
(in
kg
)
Time (day)
Average body weight
Control
FOS
0
50
100
150
200
250
300
350
400
Control Control FOS Control FOS Control FOS
0-3 3-7 7-14 14-21
Weig
ht
(in
gram
s)
Growth per day
Average daily gain
Effect on gut bacteria in colon
Treatment Diversity
Control.14 3.12 ± 0.06
FOS.14 3.16 ± 0.06
CompositionShannon diversity
Abundance of bacteria (top 10)
�No significant differences
�High variance
� Trends lower Lactobacillliand higher E.coli in piglets receiving FOS
Gene expression in jejunum
Animal Breeding & Genomics Centre
Day 14 Day 25
Cell cycle
Extracellular matrix
turn-over of
epithelial cells tight junctions /
barrier function
Immune
more energy for
intestinal tissue
higher diversity
of microbiota
No higher villi (yet)
Conclusions
FOS supplementation to suckling piglets has a
bifidogenic effect in the colon on day 14 after birth
Jejunal gene expression of FOS piglets showed lower
activity of immunological genes compared to the
controls
Increased diversity in jejunum in piglets
receiving FOS
Lower activity of cell cycle related processes,
and higher activity extracellular matrix
processes showed in piglets receiving FOS
14
25
25
14