effect of proteolytic enzymes on dye yield of silk
TRANSCRIPT
![Page 1: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/1.jpg)
EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK
PROJECT SUPERVISOR- UNDERTAKEN BY- Dr. J.N. CHAKRABORTY AJEET KUMAR – 10110001
KETAN KUNDAL- 09110004 REWA- 10110040
SHUJA HUSSAIN- 10110052
![Page 2: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/2.jpg)
Area of work
Silk is conventionally dyed with metal complex dyes for brighter
shade and excellent colour fastness. However dye strength of
dyeings (K/S) remain out of satisfaction because of bigger size of
dye molecules. In this work attempts were made to promote dye
strength of silk by using proteolytic enzymes.
![Page 3: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/3.jpg)
Problems of conventional dyeing
• During conventional dyeing maximum dye uptake is only up to 60% – 70%.
• Used dye is drained out, which causes various difficulties during waste treatment.
• Problem of emission of effluents in environment.
![Page 4: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/4.jpg)
Objective
• To increase the dye yield of silk by using proteolytic enzymes.
![Page 5: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/5.jpg)
Literature survey
• Silk is a natural fibre that is mainly composed of proteins and amino acids.
• Silk exhibits wide acceptability for all types of dyestuffs and hence a broad spectrum of colours are available for dyeing of silk fabrics.
• Silk has good affinity towards dye molecules due to its ability to form large no of hydrogen and electrostatic bonds.
• Premetallised dyes show great affinity towards protein fibres like silk.
![Page 6: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/6.jpg)
• Enzymes are biological catalyst that catalyze a reaction and are highly selective in nature.
• For silk the enzymes that are used are proteolytic enzymes like pepsin , trypsin, papain (natural enzymes) and protease (synthetically manufactured).
![Page 7: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/7.jpg)
Plan of work
• Dyeing of silk conventionally• Dyeing of silk with different proteolytic enzymes
PapainTrypsinPepsinProtease
• Studying the effects of different enzymes for different parameters
• Comparison of conventional dyeing and enzymatic dyeing
![Page 8: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/8.jpg)
Experimental plan
• Fabric used-silk (degummed)• Dyes- 1:2 Metal complex dye• Chemicals used
Acetic acid, Non ionic detergent, Sodium carbonate• Enzymes used – Proteolytic enzymes
Natural - Papain, Trypsin, Pepsin, Synthetic - Protease
• Instrument used Water bath , Open bath dyeing machine, Computer
Colour Matching, Universal Testing Machine, Shirley stiffness tester
![Page 9: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/9.jpg)
Methodology
• Conventional dyeing - Optimization of Temperature - 40 ˚C to 90 ˚C
Time - 1hr, 1.5 hrs, 2 hrs pH – 2 to 6.5
• Enzymatic dyeing - Optimization of Temperature - 30 ˚C to 90 ˚C
Time - 1hr, 1.5 hrs, 2 hrs pH – 2 to 6.5 Concentration – 0.1% to 1.0%
![Page 10: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/10.jpg)
Conventional Dyeing
Heating of water bath up to required
temperature
Introductionof dye
Introduction of pre wet
sample
Dyeing for required time
periodWashing and
SoapingDrying
![Page 11: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/11.jpg)
Enzymatic Dyeing
Heating of water bath up to required
temperature
Pre treatment of fabric with
enzyme for 10 mins
Dyeing of sample for
required timeWashing and
soapingDrying
Introduction of dye
![Page 12: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/12.jpg)
Testing
• Reflectance value• Tensile strength• Stiffness test• Fastness
Wash fastnessRubbing fastnessLight fastness
• Crease recovery test
![Page 13: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/13.jpg)
Results and Discussions
![Page 14: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/14.jpg)
• Conventional dyeing
![Page 15: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/15.jpg)
Surface colour strength (K/S)vs
Temperature of dyeing
0 40 50 60 70 80 900
0.5
1
1.5
2
2.5
3
3.5
4
4.5
red blue
orange scarlet
green
Temperature (° C)
K/S
![Page 16: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/16.jpg)
Surface colour strength (K/S)vs
Time of dyeing
0 1 1.5 20
0.51
1.52
2.53
3.54
4.55
Scarlet OrangeRed GreenBlue
Time (°C)K
/S
![Page 17: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/17.jpg)
Surface colour strength (K/S)vspH
2 2.5 3 3.5 4 4.5 5 5.5 6 6.50
1
2
3
4
5
6
7
8
orange bluegreen redscarlet
pH
K/S
![Page 18: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/18.jpg)
• Enzymatic dyeing
![Page 19: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/19.jpg)
Surface colour strength (K/S) vs
Temperature for natural enzymes for Red
0 40 50 60 70 80 900
0.5
1
1.5
2
2.5
3
3.5
4
4.5
redblueorangescarletgreen
Temperature (° C)
K/S
![Page 20: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/20.jpg)
Surface colour strength (K/S) vs
Temperature for natural enzymes for Blue
0 30 50 700
0.5
1
1.5
2
pepsin trypsin
papain conventional
Temperature (°C)
K/S
![Page 21: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/21.jpg)
Surface colour strength (K/S) vs
Temperature for natural enzymes for Orange
0 30 50 70 900
0.5
1
1.5
2
2.5
pepsin trypsin
papain conventioanl
Temperature (°C)
K/S
![Page 22: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/22.jpg)
Surface colour strength (K/S) vs
Temperature for natural enzymes for Green
0 30 50 700
0.2
0.4
0.6
0.8
1
1.2
1.4
pepsin trypsin
papain conventional
Temperature(°C)
K/S
![Page 23: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/23.jpg)
Surface colour strength (K/S) vs
Temperature for natural enzymes for Scarlet
0 30 50 700
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
pepsin trypsinpapain conventional
Temperature(C˚)
K/S
![Page 24: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/24.jpg)
Surface colour strength (K/S) vs
Concentration for Papain
0
0.5
1
1.5
2
Concentration
K/S
![Page 25: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/25.jpg)
Surface colour strength(k/s) vs
Temperature for protease enzyme
0 30 50 70 900
0.5
1
1.5
2
2.5
3
3.5
Scarlet OrangeRed GreenBlue
Temperature(˚C)
K/S
![Page 26: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/26.jpg)
Surface colour strength(k/s) vs
Time for protease enzyme
0 1 1.5 20
1
2
3
4
5
6
Sacrlet OrangeRed Green
Time(hr)
K/S
![Page 27: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/27.jpg)
Surface colour strength(k/s) vs
pH for protease enzyme
2 2.5 3 3.5 4 4.5 5 5.5 6 6.50
0.5
1
1.5
2
2.5
3
3.5
4
Scarlet OrangeRed GreenBlue
pH
K/S
![Page 28: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/28.jpg)
Surface colour strength(k/s) vs
Concentration for protease enzyme
0 0.1 0.2 0.3 0.4 0.5
0.600000000000001
0.700000000000001 0.8 0.9 10
0.5
1
1.5
2
2.5
3
3.5
4
Scarlet Orange RedGreen Blue
pH
K/S
![Page 29: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/29.jpg)
Comparison of Surface colour strength(k/s)
The dye yield of protease enzyme significantly higher than conventional dyeing.
Scarlet Orange Red Green Blue0
1
2
3
4
5
6
Conventional Protease
Shades
K/S
![Page 30: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/30.jpg)
Tensile strength of dyed fabric for different enzymes
Tensile strength of undyed sample- 201.1 N
Dyeing with enzymes lead to decrease in tensile strength of the fabric. The decrease in strength for the enzymes with respect to conventional dyeing are as follows-
Pepsin-11% Trypsin-3%Papain-2% Protease-4%
![Page 31: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/31.jpg)
Stiffness
Dyeing with enzymes decreases the stiffness of fabric as compared to conventional dyeing. The flexural rigidity of protease dyed sample is lesser than conventionally dyed samples.
![Page 32: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/32.jpg)
Crease recovery
Dyeing with enzymes leads to better crease recovery of fabric.
![Page 33: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/33.jpg)
Fastness
Wash fastness- Conventional dyeing : 3 - 4 Enzymatic dyeing : 4 – 5
Rubbing fastness- Conventional dyeing : wet – 5, dry - 5 Enzymatic dyeing : wet – 5, dry - 5
Light fastness - Conventional dyeing : 5 - 6 Enzymatic dyeing : 5 – 6
![Page 34: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/34.jpg)
Conclusion
• Optimum parameters for conventional dyeing of silk are-Time- 1.5 hours,Temperature-90˚C,pH- 5.5
• Optimum parameters for enzymatic dyeing of silk are- Pepsin, trypsin, and papain-
Time- 1.5 hours,Temperature-70˚ C,pH- 5.5Protease-
Time- 1.5 hours,Temperature-90˚ C,pH- 4.0• The results obtained by use of pepsin, trypsin and papain does
not show very significant improvement over conventional dyeing.
• The results obtained by use of protease shows significant improvement over conventional dyeing.
![Page 35: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/35.jpg)
• Dyeing with enzymes lead to decrease in tensile strength of the fabric. The decrease in strength for the enzymes with respect to conventional dyeing are as follows-
Pepsin-11%,Trypsin-3%,Papain-2%,Protease-4%• Dyeing with protease enzyme leads to increase in washing
fastness.• Dyeing with protease enzyme leads to increase in softness of
fabric and also an increase in crease recovery.
![Page 36: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/36.jpg)
References
• Reddy R M, Innovative and multidirectional applications of natural fibre: silk-a review, Academic journal of entomology, 2009,71-75
• Turbiani F R B, Tomadon J, Seixas F L, Gimenes M L, Properties and structure of sericin films- effect of cross linking degree, Department of chemical engineering, State university of Maringa.
• Gowda K N N, Padaki N V, Sudhakar R, Subramani R, Eco friendly preparatory process for silk: degumming by protease enzyme, Man -made textiles in India, Jan 2007, 28-31
• Krishnaveni V, Study on effect of proteolytic enzyme degumming on dyeing of silk, Colourage, August 2010,61-68
![Page 37: EFFECT OF PROTEOLYTIC ENZYMES ON DYE YIELD OF SILK](https://reader035.vdocuments.mx/reader035/viewer/2022062902/58eff3461a28ab65738b4607/html5/thumbnails/37.jpg)
THANK YOU