effect of microgravity on the antibiotic resistance of p. areuginosa
DESCRIPTION
EFFECT OF MICROGRAVITY ON THE ANTIBIOTIC RESISTANCE OF P. AREUGINOSA. Mitchell Hee , Jennifer Jiang, Jasmine Kuo , Annie Surman San Marino High School San Marino, California. Background. Astronaut Fred Haise infected by P. aeruginosa on Apollo 13 (1970) - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: EFFECT OF MICROGRAVITY ON THE ANTIBIOTIC RESISTANCE OF P. AREUGINOSA](https://reader036.vdocuments.mx/reader036/viewer/2022062316/56816764550346895ddc41b3/html5/thumbnails/1.jpg)
EFFECT OF MICROGRAVITY ON THE ANTIBIOTIC RESISTANCE OF
P. AREUGINOSA
Mitchell Hee, Jennifer Jiang, Jasmine Kuo, Annie SurmanSan Marino High SchoolSan Marino, California
![Page 2: EFFECT OF MICROGRAVITY ON THE ANTIBIOTIC RESISTANCE OF P. AREUGINOSA](https://reader036.vdocuments.mx/reader036/viewer/2022062316/56816764550346895ddc41b3/html5/thumbnails/2.jpg)
BACKGROUND• Astronaut Fred Haise infected by P. aeruginosa on Apollo 13 (1970)•Bacteria contaminated water supply
• Spaceflight is known to decrease human immune response•Affects T-Cell production
![Page 3: EFFECT OF MICROGRAVITY ON THE ANTIBIOTIC RESISTANCE OF P. AREUGINOSA](https://reader036.vdocuments.mx/reader036/viewer/2022062316/56816764550346895ddc41b3/html5/thumbnails/3.jpg)
PSEUDOMONAS AERUGINOSA
• facultative anaerobe• innate resistance to
variety of antibiotics• can easily develop
new resistance• often found in soil• very few nutritional
requirements• affects those with
compromised immune system or exposed tissue
![Page 4: EFFECT OF MICROGRAVITY ON THE ANTIBIOTIC RESISTANCE OF P. AREUGINOSA](https://reader036.vdocuments.mx/reader036/viewer/2022062316/56816764550346895ddc41b3/html5/thumbnails/4.jpg)
Bacteria Microgravity
?
![Page 5: EFFECT OF MICROGRAVITY ON THE ANTIBIOTIC RESISTANCE OF P. AREUGINOSA](https://reader036.vdocuments.mx/reader036/viewer/2022062316/56816764550346895ddc41b3/html5/thumbnails/5.jpg)
MATERIALSType 3 FMEMain Volume: 5.50 ml of broth medium 0.5% peptone 0.3% chicken extract 0.5% NaCl 0.5% arabinose
Short Ampoule A: 1 mg freeze-dried P. aeruginosaShort Ampoule B: 0.512 ml of 0.5 mg/ml L-Valine in distilled water
A B
![Page 6: EFFECT OF MICROGRAVITY ON THE ANTIBIOTIC RESISTANCE OF P. AREUGINOSA](https://reader036.vdocuments.mx/reader036/viewer/2022062316/56816764550346895ddc41b3/html5/thumbnails/6.jpg)
ASSEMBLY PROCEDURESAmpoule A: P. aeruginosa
Ampoule B: L-Valine
Growth Medium
![Page 7: EFFECT OF MICROGRAVITY ON THE ANTIBIOTIC RESISTANCE OF P. AREUGINOSA](https://reader036.vdocuments.mx/reader036/viewer/2022062316/56816764550346895ddc41b3/html5/thumbnails/7.jpg)
EXPERIMENT PROCEDURES• Initiation; crack Ampoule A – A+1• Termination; crack Ampoule B – A+3•Upon return to earth:•Samples from flight and home combined with isoleucine to initiate growth•Plated on agar•Allowed to reproduce overnight at 37°C
P. aeruginosa
Isoleucine
Antibiotic discs
![Page 8: EFFECT OF MICROGRAVITY ON THE ANTIBIOTIC RESISTANCE OF P. AREUGINOSA](https://reader036.vdocuments.mx/reader036/viewer/2022062316/56816764550346895ddc41b3/html5/thumbnails/8.jpg)
Streptomycin Gentamicin Ciprofloxacin0
5
10
15
20
25
30
35
The effects of ANTIBIOTICS on P. aeruginosa
Ground Flight
Zone
of I
nhib
ition
(mm
)
![Page 9: EFFECT OF MICROGRAVITY ON THE ANTIBIOTIC RESISTANCE OF P. AREUGINOSA](https://reader036.vdocuments.mx/reader036/viewer/2022062316/56816764550346895ddc41b3/html5/thumbnails/9.jpg)
ANALYSIS/CONCLUSION • P. aeruginosa is:•More resistant to streptomycin and gentamicin•Less resistant to ciprofloxacin•Remained resistant to ampicillin (ground and flight)
• Inaccuracies due to:•A mishap in experiment procedures•Broken Teflon tube
• Microgravity aids the growth of P. aeruginosa•Allows for suspension in growth medium
![Page 10: EFFECT OF MICROGRAVITY ON THE ANTIBIOTIC RESISTANCE OF P. AREUGINOSA](https://reader036.vdocuments.mx/reader036/viewer/2022062316/56816764550346895ddc41b3/html5/thumbnails/10.jpg)
FURTHER STEPS
• Measure minimum inhibitory concentration• Indicator to compare growth
•Use of spectrophotometer• Change antibiotics to correct redundancy
![Page 11: EFFECT OF MICROGRAVITY ON THE ANTIBIOTIC RESISTANCE OF P. AREUGINOSA](https://reader036.vdocuments.mx/reader036/viewer/2022062316/56816764550346895ddc41b3/html5/thumbnails/11.jpg)
ACKNOWLEDGEMENTSMr. Wyeth ColloDr. Ali KhoshnanSSEPNanoracksCalifornia Institute of TechnologyThe Smithsonian Institution