ecl presentation: microbial metabolism of contaminants ... · pdf filemicrobial metabolism of...
TRANSCRIPT
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Microbial metabolism of contaminants: from oil and pesticides in Ecuador to selenium in California’s largest lake, the Salton Sea
Juan VillaRomero, PhDEnvironmental Chemistry LaboratoryDepartment of Toxic Substance [email protected]
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Outline
• Ecuador, oil, pesticides
• Selenium in the Western US
• Selenium accumulation in Salton Sea sediment: differences by geographical location and depth
• Isolating selenate reducers
• Engineering the microbiome to address chemical contamination
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Ecuador
• Oil, ~60% of national income, lax to non-existent environmental regulations
• Aguinda vs. Chevron, 1993: US$20 billion
• Assessing cancer risk in workers from the flower and petroleum industry
• Microbial enrichments: chlorpyrifos, aldicarb, pentachlorophenol, benzene
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Ecuador: assessing risk, exploring solutions
A
B
Image credits: CellBiolabs Inc. 2016
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Outline
• Ecuador: development, lax environmental regulations
• Idaho, California: Selenium
• Selenium accumulation in Salton Sea sediment: differences by geographical location and depth
• Isolating selenate reducers
• Engineering the microbiome to address chemical contamination
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Selenium (Se)
Image credits: HM Publishing, 2016; Nature’s Way, 2016
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Image credits: Waterkeeper Alliance, 2014; Presser & Ohlendorf, 1987
Selenium (Se)
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aerobicanaerobic
Lenz & Lens, 2009
Selenium (Se)Se VI
Se IV
Se (0)
Se -IISe -II
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Nancharaiah & Lens, 2015
Se
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North America, Cretaceous period, 85 Ma
Image credits: Ron Blakey, Colorado Plateau Geosystems Inc.
Se in the Western US
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The Salton Sea, CA
• SW U.S.
• 980 km2
• 8 m average depth
• Eutrophic
• Highly productive
• HypersalineImage credits: Wikimedia Commons
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The Salton Sea, CA
• SW U.S.
• 980 km2
• 8 m average depth
• Eutrophic
• Highly productive
• HypersalineImage credits: Wikimedia Commons
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Lenz & Lens, 2009
aerobic
anaerobic
Selenate: SeO42-
Selenite: SeO32-
Se0
mobile
immobile, biologically inert organic matter
Se in sedimentSe VI: selenate
Se0: elemental
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• 5 μg L-1 (water); 5 mg kg-1 (soil)
• Se is the most notable inorganic pollutant of concern
• Se is an imminent threat to birds
• Littoral sediments will be affected by a reduction in the size of the lake
Selenium (Se) in the Salton Sea
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Research objectives
• Evaluate differences in littoral sediment across geographical locations, depth
• Quantify selenate reduction rates and kinetic parameters for selenate reduction
• Identify the sediment parameters that control Se retention at the centimeter scale
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METHODS
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Flow-Through Reactors (FTRs) to sample, characterize sediment @ cm scale
Particle size, surface area
Corg, NTOT, Se content
Abundance of selenate reducers (MPN)
Sorption
Selenate reduction rates
Km , Rmax for selenate reduction
Pallud et al., 2007
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1510505km
AB/NORTH
CD
E
F/SOUTH
GSalton City
Mecca
Bombay Beach
NEW
S
Image credits: Céline Pallud, 2015; Juan VillaRomero, 2011
FTR
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1510505km
NORTH
Salton City
Bombay Beach
NEW
S
SOUTH
Image credits: Céline Pallud, 2015; Juan VillaRomero, 2011
Kinetics of selenate reduction, differences with depth
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time
CSe(VI)
INPUT
C0
FTRs: calculating rates of SeVI reduction
OUTPUToutput [Se(VI)]
C
C
time
C0 input [Se(VI)]
intact sediment slice
Image credits: Céline Pallud, 2015
C × QVSeRR=
volumetric flow rate
volume of sediment
C between input and output
Se(VI) reduction rate
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Rmax
Concentration
Rate
0.5 Rmax
Km
CK
CRR
m
max
+=
FTRs: C vs. C/R → Km, Rmax
Image credits: Pallud et al., 2007; Céline Pallud, 2015
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Most Probable Number (MPN)
Image credits: Lenz & Lens, 2009; Wikimedia Commons
SeO42- + 6 e- Se0
Abundance estimates: number of replicates, dilutions and positives
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Most Probable Number (MPN)
Image credits: Google Earth, JF VillaRomero
SS-1
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RESULTS
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By geographical location
VillaRomero et al., 2013
G
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VillaRomero et al., 2013
(a) (b)(a)(a)
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VillaRomero 2015, in preparation
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cm µM
NORTH
0-2 448.6 16.62-4 10.0 11.54-6 100.0 8.56-8 661.0 29.2
SOUTH
0-2 15.0 10.02-4 173.8 6.44-6 420.8 11.96-8 616.1 16.5
Km Rmax
nmol/h/cm3
Kinetic parameters: Km, Rmaxby depth
VillaRomero 2015, in preparation
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Steinberg & Oremland, 1990
Kinetic parameters: Km
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Selenium accumulation in Salton Sea littoral sediments - conclusions
Littoral sediment is biogeochemically diverse: parameter, location, depth
Potential selenate reduction rates are six orders of magnitude greater than selenium input. Virtually all Se remains sequestered in sediments
Potential selenate reduction rates, highest in site with the highest Corg and N content. Role of salinity, microbial reduction vs. abiotic sorption.
Highest selenate reduction potential and highest Rmaxdo not coincide spatially
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Outline
• Ecuador: development, lax environmental regulations
• Idaho, California: Selenium
• Selenium accumulation in Salton Sea sediment: differences by geographical location and depth
• Isolating selenate reducers
• Engineering the microbiome to address chemical contamination
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Microbial metabolism: ~4 BY
Image credits: In-situ bioremediation, National Academy Press, 1993
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Microbial metabolism: ~4 BY
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Finding microbes
Image credits: Google Earth, JF VillaRomero
SS-1
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Engineering microbes
Image credits: Thodey et al., 2014; Stanford University, 2016
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Engineering microbes
Image credits: Thodey et al., 2014; Stanford University, 2016
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Engineering microbes
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Microbial removal of Se
Image credits: GE, 2016
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ITRC – Environmental Molecular Diagnostics (EMD)
Image credits: SiREM, 2016
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Engineering the human microbiome to eliminate pollutants?
Image credits: Scientific American, 2015
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Acknowledgments• Céline Pallud & Pallud Lab of Soil Biogeophysics and
Biogeochemistry• Department of Plant and Microbial Biology, University
of California, Berkeley• Environmental Protection Agency Grant STAR FP-
91727201
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Questions?
Image credits: Juan VillaRomero, 2012