dynamis™ medium introduction · 2017. 7. 21. · scale-up is designed to provide the power to...
TRANSCRIPT
-
1
The world leader in serving science
Prepared for (customer name)
BAM Name
Dynamis™ Medium Introduction
-
2
Gibco™ Dynamis™ Medium An excellent scale-up option for your CHO Cell Clones
The Power to achieve
higher titers The Power to start faster
The Power to streamline
scale-up
Is designed to provide the power to help you seamlessly scale-up
Fill with
Picture or
graphic
Fill with
Picture or
graphic
Fill with
Picture or
graphic
Dynamis means “power” in Greek
Dynamis™ medium provides
the power to achieve higher titers, start faster, and
streamline scale-up for upstream process development
and bioprocessing.
-
3
Dynamis™ medium enables highest titers at harvest
Glucose only fed-batch cultures show Dynamis medium achieved 74% higher titer compared to the next-best competitor,
with highest growth in titer from day 14 to day 21 at 30%
Internal Clone IgG Titer Comparison on Harvest Day
-
4
Dynamis™ medium enables highest titers in fed-batch culture
Fed-batch cultures show up to 60% higher titers – customer clone
-
5
Enabling a faster & more sustained start with Dynamis™ medium
0
2
4
6
8
10
12
14
16
0 5 10 15 20
VC
D (
E6
ce
lls
/mL
)
Culture time (days)
Internal IgG Clone Viable Cell Density
Dynamis™ Medium
ActiCHO P
BalanCD™ CHO Growth A Medium
Cellvento™ CHO-200 Medium
EX-CELL® CHOZN Medium
HyCell™ CHO Medium
PowerCHO™ 2
PowerCHO™-GS
Quick to reach 10E6 cells/mL and sustain that level days longer
™
-
6
Dynamis™ Medium Enables Highest Viability (Internal IgG Clone)
Glucose only fed-batch cultures show higher and more sustained viabilities
0
20
40
60
80
100
120
0 5 10 15 20
Pe
rce
nt
Via
bile
Cell
s
Culture Time (Days)
DG44 Viability
Dynamis™ Medium
ActiCHO P
BalanCD™ CHO Growth A Medium
Cellvento™ CHO-200 Medium
EX-CELL® CHOZN Medium
HyCell™ CHO Medium
PowerCHO™ 2
PowerCHO™-GS
™
-
7
AGT™ dry format simplicity helps streamline scale-up
AGT: Advanced Granulation Technology
Dynamis™ AGT dry format product offers these time saving benefits
Key Benefits
Auto pH: Just add Water
No Add backs Required; single part
Fast Dissolution and Mix Time
Dust Free Addition to Tank
Minimal Filtration Pressure Buildup
-
8
Comparable performance is seen when moving from CD FortiCHO™ medium to Dynamis™ medium
0
2
4
6
8
10
12
14
16
18
3 5 7 9 11 14
VC
D
(x 1
0e
6/m
L)
Culture Time (Days)
Dynamis AGT Medium - Internal IgG Clone Growth Comparison to CD FortiCHO Medium
Dynamis™ AGT™ Medium Lot 1
Dynamis™ AGT™ Medium Lot 2
CD FortiCHO™ Medium Lot 1
CD FortiCHO™ Medium Lot 2
CD FortiCHO™ Medium Lot 3
Dynamis medium provides a more scalable option
-
9
Comparable performance is seen when moving from CD FortiCHO™ medium to Dynamis™ medium
Dynamis medium provides a more scalable option
-
10
CD FortiCHO™ medium generated clones produce similarly in CD FortiCHO™ and Dynamis™ media
• IgG-producing clones isolated using standard Freedom CHO-S workflow (in
CD FortiCHO medium)
• Day 14 titers
• SFB is simple fed-batch, glucose feeding only per Freedom CHO-S Manual standard protocol
• FB is fed-batch culture using CD EfficientFeed C supplement for feeding without optimization of conditions
0
100
200
300
400
500
600
700
800
Clone 1 Clone 2
Tit
er
(mg
/L)
Freedom™ CHO-S™ Clones in SFB
CD FortiCHO
Dynamis
0
200
400
600
800
1000
1200
Clone 1 Clone 2
Tit
er
(mg
/L)
Freedom™ CHO-S™ Clones in FB
CD FortiCHO
Dynamis
Dynamis medium helps to provide a seamless transition to production
-
11
CD FortiCHO™ medium generated clones are stable in CD FortiCHO™ and Dynamis™ media
• IgG-producing clones isolated using standard Freedom™ CHO-S™
workflow (in CD FortiCHO medium)
• Titer determined in stability cell train
-40%
-30%
-20%
-10%
0%
10%
20%
30%
40%
0 10 20 30 40 50 60
Tit
er
Re
lati
ve
to
Ea
rly (
%)
Time (Generations)
Clone 1 Stability
CD FortiCHO
Dynamis
-40%
-30%
-20%
-10%
0%
10%
20%
30%
40%
0 10 20 30 40 50 60
Tit
er
Re
lati
ve
to
Ea
rly (
%)
Time (Generations)
Clone 2 Stability
CD FortiCHO
Dynamis
Dynamis medium does not alter clone stability
-
12
There is no significant change in product quality moving from CD FortiCHO™ medium to Dynamis™ medium
0
10
20
30
40
50
60
70
80
90
day11 - CD FortiCHO™
medium
day 11-Dynamis™
medium
day14 - CD FortiCHO™
medium
day 14-Dynamis™
medium
day17 - CD FortiCHO™
medium
day 17-Dynamis™
medium
% G
lyc
an
Sample Day - Condition
Medium Comparison - DG44 IgG Glycan Analysis
%G0F %G1F %G2F %Man5/A1F
-
13
Dynamis™ medium for seamless process development scale-up
Dynamis™ medium provides: • The power to achieve higher titers
• The power to start faster
• The power to streamline scale-up
Catalog Numbers Available: • 1L AGT™ dry format – A2617504
• 10L AGT dry format – A2617501
• 100L AGT dry format – A2617502
• 10Kg AGT dry format – A2617503
www.lifetechnologies.com/dynamis
Transfer to Dynamis medium today! Caution: For manufacturing, processing or repacking.