dvine e-juicedvinelabs.ca/content/documents/report7.pdf · 2018-11-22 · dvine e-juice 1894 scugog...

Dvine E-juice 1894 Scugog Street Port Perry, Ontario

e-Liquid Samples ECTA of Canada

Analysis Report (1115-558)

GC/FID Analysis (ENT184) Nicotine, Diethylene glycol (DEG)

HPLC/UV Analysis (ENT301) Acetaldehyde, Acetoin, Diacetyl, Formaldehyde,

2,3-Pentanedione (aka Acetyl propionyl)

General Lab Services (ENT058 & 072) pH Level, Karl Fisher Titration, % Water

Enthalpy Analytical, Inc. Phone: (919) 850 - 4392 / Fax: (919) 850 - 9012 / www.enthalpy.com

800-1 Capitola Drive Durham, NC 27713-4385

I certify that to the best of my knowledge all analytical data presented in this report:

Have been checked for completeness Are accurate, error-free, and legible Have been conducted in accordance with approved protocol, and that all deviations and

analytical problems are summarized in the appropriate narrative(s) This analytical report was prepared in Portable Document Format (.PDF) and contains 16 pages.

Report Issued: 1/18/2016

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Summary of Results

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Report for: Dvine E-juice Project Code: 1115-558 Client Project: ECTA of Canada Project Start Date: 11/16/15 Sample Type: e-Liquid Analysis Method: ENT184

Enthalpy Code Client Sample ID NotesNicotine (mg/mL)

1115-558-01 ECTA0028-0023 Menthol 24mg 21.21115-558-02 ECTA0028-0024 Menthol Tobacco 18mg 15.11115-558-03 ECTA0028-0025 Candy Floss 12mg 10.41115-558-04 ECTA0028-0026 Pipe Tobacco 6mg 6.791115-558-05 ECTA0028-0027 Bubblegum 0mg < 0.100 ND

ND: Not Detected; analyte not detected above the MDL

Testing Cert. # 3198.01

1115-558 v0.1 nic, Nico (2)Page 1 of 1 1/13/201610:08 AMEA# 1115-558 of 16

Report for: Dvine E-juice Project Code: 1115-558 Client Project: ECTA of Canada Project Start Date: 11/16/15 Sample Type: e-Liquid Analysis Methods: ENT184

Enthalpy Code Client Code Notes

Diethylene glycol

(ug/mL)

1115-558-01 ECTA0028-0023 Menthol 24mg < 14.0 ND1115-558-02 ECTA0028-0024 Menthol Tobacco 18mg < 14.0 ND1115-558-03 ECTA0028-0025 Candy Floss 12mg < 14.0 ND1115-558-04 ECTA0028-0026 Pipe Tobacco 6mg < 14.0 ND1115-558-05 ECTA0028-0027 Bubblegum 0mg < 14.0 ND

ND: Non Detect or analytical result below the MDL and is less than (<) the reported value.

1115-558 DEG v0.5, DEG (2)Page 1 of 1

1/13/201610:03 AM

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Blank Adjusted Concentrations, ug/mL

Enthalpy Code Client Code NotesAcetaldehyde

(ug/mL)Acetoin#

(ug/mL)Diacetyl (ug/mL)

Formaldehyde (ug/mL)

2,3-

Pentanedione#

(ug/mL)

1115-558-01-1 0.583 J <0.322 ND <0.296 ND 2.05 <0.352 ND

1115-558-01-2 0.551 J <0.322 ND <0.296 ND 2.07 <0.352 NDAverage 0.567 J <0.322 ND <0.296 ND 2.06 <0.352 ND

%DIFF 5.6 NA NA 0.9 NA

1115-558-02-1 2.25 <0.322 ND <0.296 ND 2.10 <0.352 ND1115-558-02-2 2.27 <0.322 ND <0.296 ND 2.16 <0.352 ND

Average 2.26 <0.322 ND <0.296 ND 2.13 <0.352 ND


1115-558-03-1 5.46 <0.322 ND <0.296 ND 1.12 <0.352 ND1115-558-03-2 5.64 <0.322 ND <0.296 ND 1.22 <0.352 ND

Average 5.55 <0.322 ND <0.296 ND 1.17 <0.352 ND


1115-558-04-1 2.22 <0.322 ND <0.296 ND 2.11 <0.352 ND1115-558-04-2 2.27 <0.322 ND <0.296 ND 2.03 <0.352 ND

Average Average 2.24 <0.322 ND <0.296 ND 2.07 <0.352 ND

%DIFF %DIFF 2.0 NA NA 3.8 NA

Menthol 24mg

Menthol Tobacco 18mg

Candy Floss 12mg

Pipe Tobacco 6mg

ECTA0028-0023

ECTA0028-0024

ECTA0028-0025

ECTA0028-0026


1115-558 ECTA HPLC Carb, Carbs<sV2Page 1 of 2 1/13/201610:05 AMEA# 1115-558 of 16


Blank Adjusted Concentrations, ug/mL

Enthalpy Code Client Code NotesAcetaldehyde

(ug/mL)Acetoin#

(ug/mL)Diacetyl (ug/mL)

Formaldehyde (ug/mL)

2,3-

Pentanedione#

(ug/mL)


1115-558-05-1 3.25 <0.322 ND <0.296 ND 1.57 <0.352 ND1115-558-05-2 3.30 <0.322 ND <0.296 ND 1.59 <0.352 ND

Average Average 3.27 <0.322 ND <0.296 ND 1.58 <0.352 ND

%DIFF %DIFF 1.5 NA NA 1.4 NA

# Acetoin is also known as 3-Hydroxy-2-butanone and 2,3-pentanedione is also known as acetyl propionyl.

ND: Non Detect or analytical result below the MDL and is less than (<) the reported value.

J: Results flagged "J" are below the Lower Curve Limit, above the MDL, and are considered estimates.

Bubblegum 0mgECTA0028-0027

1115-558 ECTA HPLC Carb, Carbs<sV2Page 2 of 2 1/13/201610:05 AMEA# 1115-558 of 16

Report for: Dvine E-juice Project Code: 1115-558Client Project: ECTA of Canada Project Start Date: 11/16/15Sample Type: e-Liquid Analysis Methods: ENT058 & 072

Enthalpy Code Client Code Notes % Water pH

1115-558-01 ECTA0028-0023 Menthol 24mg 0.99 9.501115-558-02 ECTA0028-0024 Menthol Tobacco 18mg 0.56 9.311115-558-03 ECTA0028-0025 Candy Floss 12mg 1.38 8.421115-558-04 ECTA0028-0026 Pipe Tobacco 6mg 1.53 8.771115-558-05 ECTA0028-0027 Bubblegum 0mg 1.16 5.27

1115-558 pH-KFT, sumPage 1 of 1

1/13/201610:07 AM

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Narrative Summary

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Enthalpy Analytical Narrative Summary

Company Dvine E-juice Client Proj ECTA of Canada Analysts KEH, BJB, TMS, JDB2 Job # 1115-558

Parameters ENT184, 301, 058, 072 # Samples 5 e-Liquid samples

Custody Jessica Badger received the samples on 11/16/15 after being

relinquished by Dvine E-juice. The samples were received at ambient temperature and in good condition. Prior to, during, and after analysis, the samples were kept under lock with access only to authorized personnel by Enthalpy Analytical, Inc.

Analysis (Nicotine)

The liquid samples were prepared and analyzed for nicotine and following the analytical procedures in Enthalpy SOP ENT184.

The Hewlett Packard Model 5890, Gas Chromatograph “Ricky” was equipped with a Flame Ionization Detector and an appropriate column.

Analysis (DEG)

The samples were prepared and analyzed for diethylene glycol (DEG) following the analytical procedures in Enthalpy SOP ENT184.

The Hewlett Packard Model 5890, Gas Chromatograph “Ricky” was equipped with a Flame Ionization Detector and an appropriate column.

Analysis (Carbonyls)

The liquid samples were analyzed for acetaldehyde, acetoin (aka 3-hydroxy-2-butanone), diacetyl, formaldehyde, and 2,3-pentanedione (aka acetyl propionyl) following the procedures in Enthalpy SOP ENT301.

The samples were analyzed using the Agilent Model 1100, High Performance Liquid Chromatograph “Groucho,” equipped with an Ultraviolet (UV) Detector operating at 360 nm and an appropriate column.

Analysis (pH & KFT)

Samples were measured for pH using an Orion 230A pH meter according the Enthalpy SOP ENT058. Each sample was diluted to a 1% aqueous solution with DI water.

Samples were analyzed for water content using the analytical procedures in Method FWN-4604 and Enthalpy SOP ENT072, Determination of % Water by Karl Fischer Titration. The % water results were determined on a Metrohm KFT Titrino Model 795 Titrator.

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Enthalpy Analytical Narrative Summary (continued)

Analysis (pH & KFT continued)

The titrations were performed in a dry methanol reagent. Titrant was purchased from Riedel de Haen and had a nominal titrant value of 5 mg water/mL titrant.

The 795 KFT uses an internal program and a programmable dosimeter to dispense titrant. Titrant values were read and printed to an Excel spreadsheet.

QC Notes All QC samples were within acceptance criteria unless otherwise noted.

Formaldehyde was detected in the laboratory blank above the minimum detection limit (MDL) and the results have been blank corrected.

Reporting Notes Sample and calibration curve chromatograms are available upon request.

The results presented in this report are representative of the samples as provided to the laboratory.

Please note that acetoin (aka 3-hydroxy-2-butanone), diacetyl, and 2,3-pentanedione (aka acetyl propionyl) may show false positive results via HPLC/UV in a small percentage of results. These can be confirmed via GC/MS.

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General Reporting Notes

The following are general reporting notes that are applicable to all Enthalpy Analytical, Inc. TSC data reports, unless specifically noted otherwise.

The acronym TSC represents Enthalpy’s Tobacco Smoke Chemistry department.

The acronym MDL represents the Minimum Detection Limit. Below this value, the laboratory cannot determine the presence of the analyte of interest reliably.

The acronym LOQ represents the Limit of Quantitation (aka Lowest Standard Value or Lower

Curve Limit). Below this value, the laboratory cannot quantitate the analyte of interest within the criteria of the method.

The acronym ND following a value indicates a non-detect or analytical result below the MDL.

The letter J in the Qualifier or Flag column in the results indicates that the value is between the

MDL and the LOQ. The laboratory can positively identify the analyte of interest as present, but the value should be considered an estimate.

The letter E in the Qualifier or Flag column in the results indicates an analytical result exceeding

100% of the highest calibration point. The associated value should be considered an estimate.

The acronym DF represents Dilution Factor. This number represents dilution of the sample during the preparation and/or analysis process. The analytical result taken from a laboratory instrument is multiplied by the DF to determine the final, undiluted sample result.

The addition of MS to the Sample ID represents a Matrix Spike. An aliquot of an actual sample is

spiked with a known amount of analyte so that a percent recovery value can be determined. The MS analysis indicates what effect the sample matrix may have on the target analyte, i.e. whether or not anything in the sample matrix interferes with the analysis of the analyte(s).

The addition of MSD to the Sample ID represents a Matrix Spike Duplicate. Prepared in the same

manner as an MS, the use of duplicate matrix spikes allows further confirmation of laboratory quality by showing the consistency of results gained by performing the same steps multiple times.

The Sample ID LCS represents a Laboratory Control Sample. Clean matrix (similar to the client

sample matrix) is prepared and analyzed by the laboratory using the same reagents, spiking standards, and procedures used for the client samples. The LCS is used to assess the control of the laboratory’s analytical system.

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General Reporting Notes (continued)

Significant Figures: Where the reported value is much greater than unity (1.00) in the units expressed, the number is rounded to a whole number of units rather than to 3 significant figures. For example, a value of 1,456.45 ug/mL is rounded to 1,456 ug/mL. There are four significant digits displayed, but no confidence should be placed on more than two significant digits.

Manual Integration: The data systems used for processing will flag manually integrated peaks with an “M.” There are several reasons a peak may be manually integrated. These reasons will be identified by the following two-letter designations on sample chromatograms, if provided in the report. The peak was not integrated by the software “NI,” the peak was integrated incorrectly by the software “II,” or the wrong peak was integrated by the software “WP.” These codes will accompany the analyst’s manual integration stamp placed next to the compound name on the chromatogram.

The suffix –Mod signifies that the method and/or SOP used has been modified to meet the needs

of the analysis.

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Sample Receipt Record

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(-

Chain of Custody Recordt Standard Turn Around Time (1 0 business days)

D Rush Turn Around Time - Date Needed_. All TATS Subi.ct to Approval by Enthalpy Analytical, lnc.. All Bag/Can SrmplEs Disposed of 1 Month from Receipt.

. All Othar SamdFs Disposed of 4 Months from Receipt.

Sitc N.mc: Hcad Olllce el eDh one#: 226 -626- 5040

providg sample vglumes for recoverycalculations.

For Particulates: please provide tarewalghts andror condensed water

A=Air 1=H2SO4 2=NaOH 3=_ 4=X=XAD C=Gharcoat SG=Silica Gel

G=Grab C=Composite Q=Quality Control

Menthol Tobacco 18mg

ECTA0028-0025

11106t2015

n lced k.Ambient l'Cn lced n Ambient E'Cn lced r Ambient tr oC

800-l Capltola Drlve. Durham, NC 27713. (919) 8504392. FAX (919) 850-9012. wvuw.enthatpy.com

t/-t7ls

,tuI

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dvine e-juicedvinelabs.ca/content/documents/report7.pdf · 2018-11-22 · dvine e-juice 1894 scugog...

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