Dr. Yoshitsugi Hokama Professor Emeritus of Pathology, John A Burns School of Medicine

UNIVERSITY OF HAWAI’I AT MANOA

Personal History

Date of Birth: October 25, 1925

Place of Birth: Kohala, Hawaii

Children: Two

Education:

1940 – 1943 Pahala High School, Pahala, Hawaii

1943 – 1945 University of Hawaii, Honolulu, Hawaii

1949 – 1957 University of California at Los Angeles

Bachelor of Arts: 1951

Master of Arts: 1953

Doctor of Philosophy: 1957

Positions Held:

1968 – 2008 Professor of Pathology, John A Burns School of

Medicine, University of Hawaii at Manoa

1977 – 1981 Director, Clinical Research Laboratory, Cancer Center

of Hawaii, University of Hawaii at Manoa

1974 – Consultant, Clinical Immunologist, Accupath

Laboratories, Inc., Honolulu, HI

1952 – 1966 Microbiologist, Dept. of Radiology, UCLA Health

Science Center

1964 – 1966 Associate Professor Of Microbiology, California State

College of Los Angeles

1962 – 1966 Consultant, Courtland Laboratories, Los Angeles, CA

1946 – 1949 US Army Service

1: Hokama Y, Campora CE, Hara C, Kuribayashi T, Le Huynh D, Yabusaki K. Anticardiolipin antibodies in the sera of patients with diagnosed chronic fatigue syndrome. J Clin Lab Anal. 2009;23(4):210-2. 2: Empey Campora C, Hokama Y, Yabusaki K, Isobe M. Development of an enzyme-linked immunosorbent assay for the detection of ciguatoxin in fish tissue using chicken immunoglobulin Y. J Clin Lab Anal. 2008;22(4):239-45. 3: Empey Campora C, Dierking J, Tamaru CS, Hokama Y, Vincent D. Detection of ciguatoxin in fish tissue using sandwich ELISA and neuroblastoma cell bioassay. J Clin Lab Anal. 2008;22(4):246-53. 4: Hokama Y, Empey-Campora C, Hara C, Higa N, Siu N, Lau R, Kuribayashi T, Yabusaki K. Acute phase phospholipids related to the cardiolipin of mitochondria in the sera of patients with chronic fatigue syndrome (CFS), chronic Ciguatera fish poisoning (CCFP), and other diseases attributed to chemicals, Gulf War, and marine toxins. J Clin Lab Anal. 2008;22(2):99-105. 5: Campora CE, Hokama Y, Ebesu JS. Comparative analysis of purified Pacific and Caribbean ciguatoxin congeners and related marine toxins using a modified ELISA technique. J Clin Lab Anal. 2006;20(3):121-5. 6: Hokama Y, Chun KE, Campora CE, Higa N, Suma C, Hamajima A, Isobe M. Biological activity of the functional epitope of ciguatoxin fragment AB on the neuroblastoma sodium channel in tissue culture. J Clin Lab Anal. 2006;20(3):126-32. 7: Hokama Y, Uto GA, Palafox NA, Enlander D, Jordan E, Cocchetto A. Chronic phase lipids in sera of chronic fatigue syndrome (CFS), chronic ciguatera fish poisoning (CCFP), hepatitis B, and cancer with antigenic epitope resembling ciguatoxin, as assessed with MAb-CTX. J Clin Lab Anal. 2003;17(4):132-9. 8: Wachi KM, Hokama Y. Diversity of marine biotoxins in the near-shore ocean area: presence of a palytoxin-like entity at Barber's Point Harbor, Oahu. J Nat Toxins. 2001 Nov;10(4):317-33. 9: Hokama Y, Wachi KM, Shiraki A, Goo C, Ebesu JS. The biological assessment of flora and fauna as standards for changes in the near-shore ocean environment: a study of Barbers Point Harbor. J Nat Toxins. 2001 Feb;10(1):57-68. 10: Wachi KM, Hokama Y, Haga LS, Shiraki A, Takenaka WE, Bignami GS, Levine L. Evidence for palytoxin as one of the sheep erythrocyte lytic in lytic factors in crude extracts of ciguateric and non-ciguateric reef fish tissue. J Nat Toxins. 2000 May;9(2):139-46. 11: Kan Y, Fujita T, Sakamoto B, Hokama Y, Nagai H. Kahalalide K: A new cyclic depsipeptide from the hawaiian green alga bryopsis species. J Nat Prod. 1999 Aug;62(8):1169-72. 12: Hokama Y, Nishimura K, Takenaka W, Ebesu JS. Simplified solid-phase membrane immunobead assay (MIA) with monoclonal anti-ciguatoxin antibody (MAb-CTX) for detection of ciguatoxin and related polyether toxins. J Nat Toxins. 1998 Feb;7(1):1-21. 13: Hokama Y, Takenaka WE, Nishimura KL, Ebesu JS, Bourke R, Sullivan PK. A simple membrane immunobead assay for detecting ciguatoxin and related polyethers from human ciguatera intoxication and natural reef fishes. J AOAC Int. 1998 Jul-Aug;81(4):727-35. 14: Kan Y, Fujita T, Nagai H, Sakamoto B, Hokama Y. Malyngamides M and N from the Hawaiian Red Alga Gracilaria coronopifolia. J Nat Prod. 1998 Jan 23;61(1):152-5. 15: Nagai H, Yasumoto T, Hokama Y. Manauealides, some of the causative agents of a red alga Gracilaria coronopifolia poisoning in Hawaii. J Nat Prod. 1997 Sep;60(9):925-8. 16: Nagai H, Yasumoto T, Hokama Y. Aplysiatoxin and debromoaplysiatoxin as the causative agents of a red alga Gracilaria coronopifolia poisoning in Hawaii. Toxicon. 1996 Jul;34(7):753-61. 17: Hirazumi A, Furusawa E, Chou SC, Hokama Y. Immunomodulation contributes to the anticancer activity of morinda citrifolia (noni) fruit juice. Proc West Pharmacol Soc. 1996;39:7-9. 18: Ebesu JS, Nagai H, Hokama Y. The first reported case of human ciguatera possibly due to a farm-cultured salmon. Toxicon. 1994 Oct;32(10):1282-6. Erratum in: Toxicon 1995 May;33(5):711. 19: Hokama Y, Shirai JL, Islam MA. Evaluation of bioassay for toxicity of ciguateric fish and associated toxins. J Clin Lab Anal. 1994;8(2):63-9. 20: Hirazumi A, Furusawa E, Chou SC, Hokama Y. Anticancer activity of Morinda citrifolia (noni) on intraperitoneally implanted Lewis lung carcinoma in syngeneic mice. Proc West Pharmacol Soc. 1994;37:145-6. 21: Hokama Y, Asahina AY, Titus E, Shirai JL, Hong TW, Chun S, Miyahara JT, Takata D, Muranaka A, Pang E, et al. A survey of ciguatera: assessment of Puako, Hawaii, associated with ciguatera toxin epidemics in humans. J Clin Lab Anal. 1993;7(3):147-54. 22: Hokama Y. Immunological studies using monoclonal antibodies for detection of low dalton marine toxins. Food Addit Contam. 1993 Jan-Feb;10(1):83-95. Review. 23: Hokama Y. Recent methods for detection of seafood toxins: recent immunological methods for ciguatoxin and related polyethers. Food Addit Contam.1993 Jan-Feb;10(1):71-82. Review. 24: Ganal CA, Asahina AY, Hokama Y, Miyahara JT. Characterization of marine toxin(s) in Myripristis sp. by immunological, mouse toxicity, and guinea pig assays. J Clin Lab Anal. 1993;7(1):41-5. 25: Hokama Y, Asahina AY, Shang ES, Hong TW, Shirai JL. Evaluation of the Hawaiian reef fishes with the solid phase immunobead assay. J Clin Lab Anal. 1993;7(1):26-30. 26: Hokama Y, Hong TW, Isobe M, Ichikawa Y, Yasumoto T. Cross-reactivity of highly purified okadaic acid (OA), synthetic, spiroketal East sphere of OA and ciguatoxin. J Clin Lab Anal. 1992;6(1):54-8. 27: Terada K, Suthum PA, Katsura K, Hokama Y. The effect of megestrol acetate on interleukin-1 activity. Gynecol Oncol. 1991 Dec;43(3):260-1. 28: Fasano A, Hokama Y, Russell R, Morris JG Jr. Diarrhea in ciguatera fish poisoning: preliminary evaluation of pathophysiological mechanisms. Gastroenterology. 1991 Feb;100(2):471-6. 29: Terada K, Hayashi G, Hokama Y. C-reactive protein and 6-keto prostaglandin F 1-alpha in patients with gynecologic cancer. Gynecol Oncol. 1990 Feb;36(2):212-4. 30: Hokama Y. Simplified solid-phase immunobead assay for detection of ciguatoxin and related polyethers. J Clin Lab Anal. 1990;4(3):213-7. 31: Hokama Y, Asahina AY, Hong TW, Shang ES, Miyahara JT. Evaluation of the stick enzyme immunoassay in Caranx sp. and Seriola dumerili associated with ciguatera. J Clin Lab Anal. 1990;4(5):363-6. 32: Hokama Y, Asahina AY, Hong TW, Katsura K, Shang E, Miyahara JT, Sweeney JC, Stone R. Causitive toxin(s) in the death of two Atlantic dolphins. J Clin Lab Anal. 1990;4(6):474-8. 33: Okamura JM, Miyagi JM, Terada K, Hokama Y. Potential clinical applications of C-reactive protein. J Clin Lab Anal. 1990;4(3):231-5. Review. 34: Kodama AM, Hokama Y. Variations in symptomatology of ciguatera poisoning. Toxicon. 1989;27(5):593-5. 35: Hokama Y, Scheuer PJ, Yasumoto T. Effect of a marine toxin on human peripheral blood monocytes. J Clin Lab Anal. 1989;3(4):215-21. 36: Kodama AM, Hokama Y, Yasumoto T, Fukui M, Manea SJ, Sutherland N. Clinical and laboratory findings implicating palytoxin as cause of ciguatera poisoning due to Decapterus macrosoma (mackerel). Toxicon. 1989;27(9):1051-3. 37: Campbell B, Nakagawa LK, Kobayashi MN, Hokama Y. Gambierdiscus toxicus in gut content of the surgeonfish Ctenochaetus strigosus (herbivore) and its relationship to toxicity. Toxicon. 1987;25(10):1125-7. 38: Hokama Y, Su DW, Skinsnes OK, Kim R, Kimura L, Yanagihara E. Effect of C-reactive protein, PHA, PWM and choline phosphate in 3H-thymidine uptake of leukocytes of leprosy patients and normal individuals. Int J Lepr Other Mycobact Dis. 1974 Jan-Mar;42(1):19-27. 39: Nishimura ET, Ross E, Leslie G, Yang HY, Hokama Y. Epizootic reticulum cell sarcoma in a sequestered colony of Japanese quails. Cancer Res. 1970 Aug;30(8):2119-26.

BIOSKETCH

Cigua-Check Fish Poison Test Kit

PUBLICATIONS

honoluluadvertiser.com Thursday, June 28, 2007

UH professor recognized for chronic-fatigue study

Advertiser Staff

Pathology professor Yoshitsugi Hokama, at the University of Hawai'i's

John A. Burns School of Medicine, has received a national award for his

research connecting ciguatera poisoning — food poisoning tied to eating

contaminated fish — to Chronic Fatigue and Immune Dysfunction

Syndrome (CFIDS), according to a press release.

The National CFIDS Foundation awarded him with the 2007 Outstanding

Researcher Award. His research was the first to find ciguatoxin, a potent

neurotoxin, in the blood of Chronic Fatigue Syndrome patients.

Since announcing his discovery at a 2002 medical conference in Japan,

Hokama secured an international blood permit so that millions of patients

with the disease could get tested by the medical school.

Ciguatoxins are recognized as some of the most potent biological toxins.

The National CFIDS Foundation, Inc. was founded about 10 years ago

with the primary goal of funding medical research to find a cause,

expedite treatments and eventually a cure for the disease.

The National CFIDS Foundation

awarded UH pathology professor

Yoshitsugi Hokama with the 2007

Outstanding Researcher Award for his

research connecting ciguatera

poisoning — food poisoning tied to

eating contaminated fish — to

Chronic Fatigue and Immune

Dysfunction Syndrome.

UH School of Medicine