dr caroline pennington chief scientific officer rse/bbsrc business enterprise fellow telephone:...
TRANSCRIPT
Dr Caroline Pennington
Chief Scientific OfficerRSE/BBSRC Business Enterprise
Fellow
Telephone: 01603 591579
Email: [email protected]
Website: www.genid.co.uk
Cell Authentication and Validation
A Case of Mistaken Identity:
Cell culture cross contamination is a long standing problem in laboratories worldwide
‘Major trials of drugs in doubt’ (BBC news 2010)
A case of mistaken identity: An Example:
An Example:
An Example:
£62K!!
A Call for Action:
Eradication of Cross Contaminated Cell Lines:Roland M Nardone
‘1. It is proposed that government and private funding agencies be prevailed upon to require cell line authentication as a condition for the award of grant and contract funds.
2. It is also proposed that key scientific journals be prevailed upon to require cell line authentication as a condition for publication.’
9. Cell Line Authentication. The authentication of cell lines is considered an important part of the preliminary data described in any grant application. Applicants should also ensure that all cell lines to be used during the proposed research are adequately authenticated
9. Cell Line Authentication. The authentication of cell lines is considered an important part of the preliminary data described in any grant application. Applicants should also ensure that all cell lines to be used during the proposed research are adequately authenticated
The First Independent Cell Validation Service
Professor Dylan Edwards
Management Team:
UEA Management Consultancy Ltd
Caroline Pennington
Single Nucleotide Polymorphism (SNP) Panel Cell Validation
AGCAGGACA
HeLa cells
AGCATGCCA GGATTGCTA
MCF7 cells U251 cells
at the commencement of a project and then at six monthly periods to ensure cells remain uncontaminated.
to ensure batch to batch identity
following long term storage and genetic manipulation of cultured cells
in multi user laboratories
for cells obtained from inter laboratory exchange
for newly generated cell lines
following ‘cell rescue’ cell sorting etc
Cell check recommendations
Business Model
ContractsBioManufacturing, stem cell Co. Pharmaceutical
R&D
BespokeBioManufacturin
g, Academic institutes,
Pharmaceutical R&D
CollaborationATCC, ECACC/HPA
ImmunosolvFACs
Academic institutes
BioTech(UK, Europe, US)
Training and support
Gene expression profiling
For the future:Cell mycoplasma
testingSpecies specific Id
TaqMan Low Density Array servicing
Bespoke Expression profiling
TIMP3/18S
020
406080
100
120140
PZPf1R +
vecto
r
PZPf1R kn
ock o
ut p
53
PZPR mut
ant p
53
PZPf1R +
175
PZPf1R 05
490
PZPf1R 92
752
PZPf1R 05
496
PZPf1R 38
222
PZPf1R 14
006
PZPR 880
00
PZPR 250
57
PZPR 304
52
PZPR 225
28
PZPR 803
96
Cell line RNA
PDAC RNA KO p53 no metastasesPDAC RNAmutant p53 metestatic PDAC
Normal Cancer
Normal Margin Centre
BPH Cancer
Breast
Prostate
ENT
Breast
Gene Expression Profiling:From Tissue to Data
RNA
Bacteria
Plant tissue
Human tissue
Mouse tissue
Rat tissue
Cultured cells
miRNA
Pharmaceutical R & D
Biomanufacturing – 70% of companies contacted express an interest
Avecia, Eden Biodesign ‘add to quality assurance in bioproduction and ….it would be an advantage that they could offer and pass on to their customers.’
Academic research institutes - interest in investigating batch to batch variation and in getting papers published and funding
released
Cell suppliers
The market
Experience, Expertise and reputation
– 10 years experience
– Over 60 collaborators worldwide
– 72 published papers and 3 book chapters
– coordinated a 5 year European Union Programme involving 41 Partner laboratories in 13 countries.
Independent validation
Working to ISO accredited procedures
A contracted or flexible bespoke services
