Download - Specimen receiving
Biochemistry Lab
Specimen Receiving
Mr. MOHAMED A. ADAMMedical Lab Technologist
KCCC
LABORATORY WORK FLOW
Samples Received with
requests
Centrifugation of samples
Data are entered in the LIS to get
barcodeBarcodes are
stick on the tubes then distributed
to machine
Sample Processing
Results are Reported and
Released
Clean your
bench with fresh actichlor
WHAT WE ARE RECEIVE?
•Specimen type
• Whole Blood
1. Plasma
2. Serum
• Body Fluid
1. Cerebro-spinal Fluid CSF
Cerebrospinal fluid (CSF) is a fluid that surrounds the brain
and spinal cord.
CSF acts as a cushion, protecting the brain and spine from
injury. The fluid is normally clear same as water.
WHAT IS BLOOD
Phlebotomist draws sample
1.Gentlly invert five timesTo mix dot activator with blood
2. Allow blood to clot in a vertical position
Observe a dense clot
3.Centrifuge for 10 min. at 3000 rpm
SEPARATION OF SAMPLES (SERUM)
PRE-ANALYTICAL3000 rpm
10 minShould be balanced
WHAT IS THE DIFFERENCE BETWEEN SERUM AND PLASMA
VACUTAINER BLOOD COLLECTION TUBESRange Color coding Additive Clinical Use for
Whole bloodEDTA
Hematology testSpecial biochemistry (HbA1c)
Immunochemistry (Cyclosporin and Tacrolimus)
PlasmaLithium Heparin Cardiac Markers
Sodium Fluoride + Potassium Oxalate Glucose Test (FBS free blood sugar)
Sodium Citrate Coagulation test
SerumPlain (NA additive)
Clot activator
Serum biochemistry and immunology testTumor Markers
HormonesVitamin D (should be covered)
Oncology RFT – LFT – Lipid profileGel + Clot activator
SAMPLE TRANSPORT AND STORAGE
• Transport must be save
Biohazardous material
• 4 HOURS Stable at 15-25 ˚c
Close to avoid evaporation
• Stable at 2-8 ˚c
Dry Ice – Cool packs – refrigerator .. etc
24 hours only (for some biochemistry analyte such as K, )
LIST OF TESTS IN OUR LABTUMOR MARKERS
Test Diagnosis
CEA (No Spicificity) Colorectal, gastrointestinal,
lung and breast cancers
CA-15.3 Breast Cancer
CA-125 Ovarian Cancer
CA-19.9 Colorectal and Pancreatic
cancer
B2Microglobulin Blood cell cancer
Test Diagnosis
PSA
Total and Free
Prostate Cancer
AFP Liver Cancer
Cyfra 21-1 Lung Cancer
TG Thyroid Cancer
ATG
B-HCG Chorion Cancer
“Pregnant test”
LIST OF TESTS IN OUR LABBIOCHEMISTRY
Biochemistry Profiles
Oncology (biochemistry _ Full Profile)
Liver Electrolyte
Lipid Amylase
Renal Iron
HbA1c
Cardiac MarkersCK – CKMB - Troponin
Thyroid Function Test (TFT)TSH – FT4
Other Tests
Vitamin D
PCT
Cyclosporin
Tacrolimus
EACH SPECIMEN SHOULD BE LABELED WITH PATIENT`S INFORMATION
• Full Name• Age• Gender• File number or Civil ID• Doctor`s Stamp and signature• Clear and Specific test should be written• Cashier Stamp (If needed) for Vitamin D , Hormones and TFT
The personal information such (Name, file # or civil ID) MUST MATCH the same on the request