Selected Experiments in Biochemistry
BIOC4107
IUG, Spring 2014TMZ
• InstructorDr. Tarek M. Zaida
• ContactOffice: Administration Building B304Office Phone: 2697 (ext.) mobile: 059-7193-442
• Email: [email protected] or [email protected]
• Office Hours• Sat & Mon: 11-13• Sun, Tue, Wed: 10-11
• Course DescriptionThis course is designed for the students with a biochemistry major, and is intended to focus on some carefully selected important protocols, and techniques used widely in biochemistry lab.
Course Objectives
1. This course aims to provide the students with an opportunity to develop their qualitative analytical skills.
2. It is expected that students upon completion of this course, will have a sound knowledge on advanced protocols applied widely in biochemistry.
3. Students will get adapted to team work.4. Helping students developing their communication skills.5. At the end of this course students will be familiar with
“how” to prepare reports on lab activities.6. Encouraging students to think critically and know how
to solve problems.
Course Topics
• Protein Dialysis, • Determination of protein pI,• Protein salting out,• Enzyme kinetics,• Aerobic respiration, • anaerobic respiration, • Bradford test, • Separation of blood serum lipids by means of Thin Layer
Chromatography,• Color Reactions of Plant Pigments, and • SDS-PAGE.
Type of Course Teaching
• This is a practice course that requires:- short lectures,- in-lab practical experiments,
and - Participation in different electronic learning tools.
Prerequisites
• Practical Analytical Chemistry CHEM2110• Biochemistry I BIOCA2301• Biochemistry II BIOCB2301• Practical Biochemistry I BIOCA2101• Practical Biochemistry II BIOCB2101
• Textbook(s)A. Main textbook:Selected Experiments in Biochemistry (Prof.
Ahmad Thabet 2010)B. Additional textbookPractical Medical Biochemistry (Tarek M. Zaida,
2011)
Course TimingExperiment Activity
1st Lab Activity Protein Dialysis Report 1
2nd Lab Activity Determination of Protein pI Report 2
3rd Lab Activity Protein Salting out Report 3
4th Lab Activity Enzyme Kinetics Report 4, Quiz 1
5th Lab Activity Aerobic Respiration Report 5
6th Lab Activity Anaerobic Respiration Report 6
7th Lab Activity Bradford Test Report 7, Quiz 2
8th Lab Activity Separation of Blood Serum Lipids by TLC Report 8
9th Lab Activity Color Reactions of Plant Pigments Report 9
10th Lab Activity SDS-PAGE part I Report 10, Quiz 3
11th Lab Activity SDS-PAGE part II Report 11
• EvaluationThere will be different types of evaluation that will include:
1. Reading the lab manual before the lab activity day2. Answering the pre-lab questions during lab session, 3. Quizzes (3)4. Writing Lab reports5. Participation in the actual lab work.6. Participation in the discussion with classmates
through the electronic learning tools.
• Course Policy- Attending all lab sessions is a mandatory.- Active engagement in different elearning tools (online quizzes, wikis, forum, chat, electronic reports). - Zero tolerance for not following given instructions during lab sessions.- Wearing the lab coat as soon as you step in the lab.- Acting responsibly during the entire lab session.- Team work is a must, including communication with group partners.- Giving up reports from the previous lab session must be electronically only.- No make ups are allowed.
• Lab Safety/Health It is my job to keep my dear students safe,
and protect them against any type of danger that might be anticipated during the lab sessions due to the different kinds of hazardous materials that might be necessary for some experiments.
However students are expected to comply completely with lab’s safety rules, and will be held responsible for their own safety once they don’t follow instructor’s rules.
Grading
Reading the lab manual before the lab session and answering the pre-lab questions during lab session.
5
Quizzes (3) 15
Lab reports (electronically submitted) 10
Active engagement in the actual lab work 20
Being active in different elearning tools 10
Final exam 40
Total Grade 100
• Following up with the Course- Students may reach the course materials through moodle system anytime. In case of anticipating any type of problems, student may contact me at my mobile or via email anytime.
• How to use moodle system- Students may go through the moodle system using guide found on the website of electronic learning http://elearning.iugaza.edu.ps , under educational resources for students.- Students may also ask for assistance from the electronic learning center found in the IT building I400, phone 1573.
