Transcript

Hydrolyzed DNA regaining the beauty with the vitality by stopping the clock of aging.After we have studied the source of life, DNA, for a long time, we have made the innovation of genetic dermatology for healthy skins. Beauty starts from healthy and powerful skins.

HYDROLYZED DNA CELL POWER, ANTI-AGING, MOSTURIZING SOLUTION NUCLEO

DNA CARE SOLUTIONFOR YOUR BRAUTY & HEALTH

www.nucleo.co.kr

中文ENGLISH

Pores Sebaceous glands

Abandonment of layer

Granular Visible layer

The bottom layer

Tube glands

Coliseum hair roots

Fiber

Sweat gland

Fur pouch

cuticle

Dermis

Sub

cuta

neo

us

tissu

e

Thin skin, an enlarged picture EpidermisParts in contact with the outer surface = there are no skin blood vessels and nervous tissues on the surfaceIt consists of five layers such as stratum corneum, stratum lucidum, stratum granulosum, stratum spinosum and stratum basale. Closely related to cosmetics

DermisLayer made up of the skin = skin = including blood vessels, nerves, lymphatic duct, sweat gland, sebaceous glands, etcIt is made up of collagen, elastin, fibroblasts and substrates. It is closely related to skin elasticity.

Subcutaneous fatIt is present in the skin at the lowermost layer. It is closely related to hormones.It protects internal organs from external physical shock and maintains the body temperature.

irritantsmicro-organisms, allergens

irritantsmicro-organisms, allergens

The structure of the skin

What is Hydrolyzed DNA ?

Decrease in activity of skin cells – aging skin

What is the solution to figure out skin aging and skin troubles ?

Normal skin vs atopic skin

Various irritants easily enter the skin of patients with atopic dermatitis and cause inflammation and immune responses. Moisture of skin is easily evaporated (TEWL increase)

The most important cause for itching and dry skin which are common symptoms of atopic dermatitis is the damage of skin barrier which is a skin protective film.

Normal skin plays a protective role against various external irritants and prevents evaporation of moisture on the skin.

You must let skin cells of the dermis by waking up dormant skin cells.To restore the physiological and biochemical homeostasis which is the normal function of skins,- Cell regeneration, activation of cell production, healthy cells- Promotion of collagen production- Improvement on moisturizing power- Young skinNucleic Acid (DNA) multiplies fibroblasts, and Increased fibroblasts activate the collagen production.In turn this process improves skin firmness.

Hydrolyzed DNA_Oligo(Low molecular weight, hydrolysis) DNAIt is the ingredient to improve the permeability into the skin by specially treating DNA with enzymes and making it in low molecular weight.NUCLEO ATO-DNA product is the world’s first cosmetics to implement double moisturizing on inner and outer skins and improve the permeability into the skin by compounding DNA-Na in high molecular weight and DNA (low molecular weight and hydrolyzed DNA).Experimental results are obtained in which the skin texture gets much better when Hydrolyzed DNA is used. In addition, it has the effects to promote the reduction of wrinkles and increase in sebum moisture.In addition, it helps to cure wounds or activates metabolism by activating the cells. It also acts as the anti-oxidant function in deep skin. Fresh and healthy skins are made by improving turnover of the skins.

Key Effect Field of Application Passing the stability test

MoisturizingSkin cell regenerationSebum controlExfoliatingAnti-wrinkleAnti-freckle Anti-age pigmentSun block

Anti-aging lineMoisturizer for drynessCalming troubled skin All-in-one gel Hair growth promoter toothpaste

Eye-irritant testAcute toxicity testFirst skin-irritant test Back mutation test

TH,immunantwort

Langerhans cells Langerhans cells

Inflammation/exacerbation

of AD

DoubleMoisturizing

Cell

HYDROLYZED

CollagenDNA

General moisturizing ingredients HYDROLYZED

DNA

MAGICAL 'S ADVANCEDDEMATOLOGICAL TECHNOLOGIES...IT IS

HYDROLYZED DNA Micro-Molecular Standard Material Comparison of competitive ingredients

A salmon extract, refined DNA, becomes micro-molecular and improves in functionality to make it proper cosmetic mixed ingredients

asement membrane peptides (Hexapeptide)Six amino acid sequences which are shared by collagen-4 and collagen-17 composing the basement membrane are synthe-sized in peptides.Wound is recovered by rapidly regenerating the basement membrane which is the basis of differentiation of the epidermis. Regeneration effects are confirmed in cells, artificial skins and clinical tests.

Peptide (Various polypeptides consisting of amino acids)Most raw materials are peptides which mimic the functional groups of proteins present in the body. Each raw material contains various effects. In general, if it has more amino acids composing peptides, it is expensive and unstable.It mostly consists of 3 – 4 amino acids. Not many peptides show the actual regeneration effects.

Snail slime (Allantoin, collagen, elastin, glycolic acid, vitamin A, D, E)Allantoin, Glycolic acid : substantial effects on patients with large burns. Burn scabs are promptly removed. Promotion of peeling of the epidermis (removal of dead skin cells), promotion of cell proliferation, removal of necrotic tissue and moisturizingContaining 80 % as raw material in Elicina cream – there is no ingredient giving the direct regeneration effect. However, it is used for moisturizing after the skin treatment, because of the sense of moisture caused by the sense of membrane of Snail slime. However, it does not have the regeneration effect at all.

EGF(Recombinant Human Epithermal Growth Factor)Epidermal growth factor _ It represents a broad skin regenera-tion effect, but it does not penetrate into the skins because of very high molecular weight.It can be degraded by proteolytic enzymes present in the skin. It is toxic at high concentration.

Registered name in INCI as cosmetic mixed ingredients:Hydrolyzed DNA Indication of Name : hydrolysis DNA-Na

Hair growth promoter effect by Hydrolyzed DNA

Certified for japan patent as a growing hair, hair growth promot-er and anti-hair loss

Growing hair root cells and hair growth promoter

Hydrolyzed DNA increase fundamental power of hair root cells

Hair

Hair root

Sebaceous glands

Hair root cells

Hair papilla

Blood vessel

Efficacy data as cosmetics of oligo DNA

Not containingHydrolyzed DNA

Before use After 4 weeks Before use After 4 weeks Before use After 4 weeks Before use After 4 weeks

ContainingHydrolyzed DNA

Inhibitory effect on capacity of collagen production

Hydrolyzed DNA shows absolutely higher capacity of collagen production compared with other moisturizing ingredients.

Normal DNA

Not containingHydrolyzed DNA

ContainingHydrolyzed DNA

Not containingHydrolyzed DNA

ContainingHydrolyzed DNA

Hydrolyzed DNA

Skin damage is reduced by significantly inhibiting the increase of Hydrolyzed DNA caused by UVB.

Amount of moisture retention in the skin

Result of comparison between states of the skin after applying cream containing Hydrolyzed DNA and cream not containing Hydrolyzed DNA onto half of face twice a day for 4 weeks.

Calculation of relative ratio of amount of collagen production / number of cellsConcentration of the applied sample : 1mg/ml, treatment time : 24 hours

Skin density

Inhibitory effect on amount of collagenase

HydrolyzedDNA

NormalDNA

Collagen Vitamin Cderivatives

Hyaluronicacid

Control

Improvement effect on atopic dermatitis

No treatment Application of ointmentcontaining oligo DNA

BEFORE AFTER

Disease of thickened skin by scratching the skins.Dry skin accompanying pigmentation (Erythroderma and pruritic exanthema are recognized)Pigmentation is reduced and state of skin disease is improved.

Effect on healing the wound by Hydrolyzed DNA

Effect on healing the wound by Hydrolyzed DNA

Ointment containing Hyarolyzed DNA is applied to 10 cm of head part in half portion of about20Cm of wound. After 7 days, the states of treated and not treated areas are compared. Recovery of wound without treatment is slow and scrab still does not fall off yet. Difference in the wound healing effect is evident

Immediately after wound

Application of ointment containing oligo DNA

No treatment

※ Amount of usage of ointment containing 3 % oligo DNA : 10.5 g = (1.5 g x 1 time) x 7 d

After 24hours After 7days

After 24hours After 7days

ROBOSKIN / ANALYZER TYPE A Left , oil and water analyzer Wave Cyber WSK-P500U

Right, microscope (skin testing) MicroscopeM2 SCALAR

SKIN TEST FOR COSMETICS OF NUCLEO ATO-DNA

Test Set : skin analyzer by robot skin

Monitor test of sensitive skin and senile dry skin Test materials :DNA lotion + DNA cream

PoreVisible number of pores Visibly large poresVisibly the number of dark pores

Darkeningsmall (number, surface)large (number, surface)

WrinkleThe number of wrinkles below eyesThe number of wrinkles on eye rim (length, surface)

Skin colorSkin color(0∼360)Radiance(0∼100)Skin clearity(0∼100)

Skin textureSkin texture(0∼100)Skin density

glowingwater measurement(0∼100)oil measurement 0∼100)

Participants

Trial period

Test time

7 people (3 of 60’s, 1 of 50’s、3 of 30’s)Sensitive skin type 4 peopleHealthy skin type 3 people

Elapsed time observed : 5daysApplied time : 14daysElapsed time observed : 7days

Elapsed time observed : Before 5days, 1 dayApplied time : After 3, 7, 10, 14daysElapsed time observed :After 3, 7Days Total 8 times

Moisture・sedum measurement Realistic assessment

The middle of inside forearm 5cmApply one, non-apply an other

1time a day (night)On the test part、skin trouble part

Test time:After 10AMTemperature : 19~27 degrees CelsiusHumidity:40~51%

Test item

Testcondition

Test item

Apply

Before 5days

After3days

After7days

Before 1days

3days 7days 10days 14days

Non-applied Non-appliedApplied

Results of moisture measurement (average of all participants)

Test result of moisture and sebum (average of all participants)

Moisture (0~100)Capacitance of the skin is determined by tightly pressing the center and it is measured with a value representing the moisture. State of measuring physiological saline itself is assigned as “saturation state (100)”, State of no moisture is assigned as “zero state (0)”.

During the application period (from the 3rd day of application to the 14th day of application), oil and moisture levels tend to be improved during the measurement period. There is no difference caused by age in 30s and 50s or older. There are no significant differences in tendency between four people who consider it as sensitive skin and three people who consider it as healthy skin.There are two monitors who feel itchy is improved among three monitors who perceive itching.

Result of measuring sebum content (average of all participants)

Sebum (0~100)Rolled sebum is determined with the refractive index by tightly pressing the sensor part and the area is measured.State of sebum covering the entire side is assigned as “sebum saturation state (100)”,State of no sebum is assigned as “sebum zero state (0)”.

Moisture value (average) Sebum value (average)

Reactive oxygen species (free radical) is considered as an aggravating factor of skin disease atopic dermatitis such as itching getting worse as of today and eczema lesion which repeats the temporary improvement.Oligo DNA with high permeability which plays roles of strong antioxidant, moisturizing activity and cell recovery, DNA-Na working on the skin surface and sphingomyelin which is the source of ceramide having anti-inflammatory effects are mixed.Nomura medical clinic reviewed the improvements on atopic dermatitis by applying cream containing oligo DNA based on symp-toms to patients suffering from atopic dermatitis.

Severity of the skin diseases : The nature and state of skin diseases are determined by observing the degree of skin state of individu-als according to the following items.

Items of skin diseasesPruritus (itching), wetting (muddy), red spots (erythema), edema (swelling), thickening skin that (thickening skin as a chronic skin disease), pigmentation, rough skin (dry), scratched wounds, skin rash (rash bumps on the skin), moisturizing ability

Criteria to determine the degreeHigh : Lesion with highly worsening skin disease items Intermediate : Lesion with intermediate degree of lesion items rather than high degree Low : Lesion with slight hardness and inflammation symptoms compared to that with intermediate degree of lesion No: No changes in lesion symptoms (not worsening and not improving)

Test result by age

Test methods and judging criteria for monitor of patients with atopic dermatitis

Moisture value (average of over 50’s)

Sebum value (average of over 50’s)

Moisture value (average of 30’s)

Sebum value (average of 30’s)

IgE levelIn addition to type I allergic reaction, high level for antibody pollinosis allergic atopic dermatitis specifically responding to allergen is shown. Eosinophilic granulocyteIt is kind of white blood cells and increases as upon occurrence of allergic diseases.LDHIt is lactic dehydrogenase and it increased at the time of damage of cells, tissues or organs. In addition, it also increases when the erythrocyte membrane is damaged. Increased reactive oxygen species is also thought to be one of the causes

Oxidative stresses caused by reactive oxygen species (free radicals), dry blood (blood fragility test)�Professor Dr. Ockerman (Sweden) used judging criteria for reactive oxygen species activity.In the attached table, a drop of blood is collected and placed on a glass slide. It is observed under a microscope. Scale of reactive oxygen species (Free Radical activity)

• Improvement effect on atopic dermatitis is recognized. In particular, the improvement effect of moisturizing ability is recognized in all skin diseases. •90 % of improvement effects on rough skins and itching is shown. 80 % of improvement effects on scratched skins is shown.•90 % of improvement effect in hardening skin except the fifth one where hardening is originally not shown is recognized.•Because hardening skin accompany chronic process, improvement tendency caused by the use of it for 4 weeks is valuable. •60 % of reduction of pigmentation is recognized.

Evaluation of clinical blood tests (academic allergy tests)

Clinical result of improvement on skin disease symptoms before and after the use

0∼5 Normal

6∼10 Very little mild oxidation stress

11∼15 Mild oxidation Stress

Moderate oxidation stress

High oxidation stress

Very high oxidation stress

16∼25

26∼35

36∼50

Examples of diseases Itchy

skin Swelling of the

skin Rough dry skin

Scratched damaged skin

Red skin Thickening skin

Pigmentation skin

Rash bumps on the skin

Moisturizing ability

NO. Sex Age (%)

Before After Before After Before After Before After Before After Before After Before After Before After Before After

1 W 36 9 2 No No High No Middle No Middle No No No Middle Slight Slight No 22.5 35.3

2 W 26 6 3 Slight No Middle Slight Slight No Slight No Slight No Slight Slight Middle Slight 23.1 30.0

3 W 38 6 2 Slight No Middle No Middle Slight Middle No Middle Slight High Slight No No 29.0 35.5

4 W 34 5 1 No No Middle No Slight No Middle No Slight No Slight No Slight No 20.1 32.1

5 W 36 8 0 Slight No Middle No High No Middle No No No Middle No High No 27.3 33.5

6 W 33 7 5 No No Middle Slight Slight Slight Middle Slight No No Slight Slight Middle Slight 30.9 36.6

7 W 32 8 8 No No Low Slight Slight Slight Slight Slight No No Slight Slight Slight Slight 36.1 39.6

8 M 32 5 1 Slight No Middle Slight Middle No Middle Slight Slight Slight High Slight No No 19.9 24.0

9 M 32 6 2 No No Low No Slight No No No No No No No Slight No 28.5 41.5

10 M 34 6 1 Middle Slight Middle Slight Slight No High Slight High Slight Middle Slight No No 27.6 30.6

Excellent effect : Symptoms are surprisingly improvedEffective : symptoms are clearly improvedInvalid : there is no improvement in symptoms

Effect of cream containing oligo DNA on improvement of atopic skin diseases is proven. As shown in the following figure, 90 % of monitors recognize the effect of improvement on skin diseases.

Comprehensive evaluation of clinical outcome

•As the allergy academic observation, a total of IgE level, eosinophilic granulocyte, LDH level and reactive oxygen species are added and reviewed. •As examples of atopic skin diseases representing chronic process are subjects, high level of total IgE is shown and a significant differ-ence is not shown.• Improvement in eosinophilic granulocyte and LDH level is recognized before and after the use.•For reactive oxygen species caused by Dry Blood, it shows 35 and medium ~ high oxidative stress state in all skin diseases before the use. However, the average level is 30, which is decreased by 5 after the use. •Reduction of oxidative stress is recognized.

From compounding ingredients of 「oligo DNA cream」, three kinds of improvement effects on major skin function are largely shown. Maintenance of skin functions, anti-inflammatory action and anti-oxidant action are given by activating epidermal cells of skin with both oligo DNA and DNA-Na. Action is taken to correct the confusion of side balance of allergic diseases. Production of ceramide acid which is moisturizing ingredient is promoted by metabolism of sphingomyelin.In addition, moisturizing action caused by collagen (improvement of moisturizing ability increases the function of forming skin film)It is thought that improvement effect on atopic skin diseases is caused by inhibition of allergic inflammation and enhancement of immunity with above three improvement actions of skin function.

Clinical result of improvement on clinical blood test before and after the use

NO.

Examples of diseases

Gross IgE IU/mℓ Eosinophile LDH IU/ℓ Dry Blood

Sex Age Before After Before After Before After Before After

1 F 36 4360 4043 430 670 249 466 30 25

2 F 26 4850 5400 240 260 348 412 38 35

3 F 38 1075 928 140 130 333 172 38 34

4 F 34 6300 6600 870 1070 201 195 38 30

5 F 36 399 400 460 320 316 271 35 30

6 F 33 76 74 180 190 224 228 37 37

7 F 32 8330 8750 1000 1090 222 191 35 37

8 M 32 5260 5420 430 470 213 207 23 22

9 M 32 8350 7970 430 340 197 203 35 27

10 M 34 1194 1177 2360 454 434 235 37 18

Average 4019 4076 654 454 274 235 35 30

Case of taking nucleic acids and using cream containing DNA

List of those who experience nucleic acids + DNA cream and comparison before and after administration (Test from the end of October, 2013 to January 28th 2014)

Kim, University student (female), Taking it on 19-Nov-2013 / Start the use

Uhm, Middle scholo student (girl), back part of neck Taking it on 27-Nov-2013 / Start the use

Choi, Primary school student (boy), neck part, Taking it on 24-Nov-2013 / Start the use

Choi, Primary school student (boy), chest part, Taking it on 24-Nov-2013 / Start the use

Taking it, before using it19-Nov-2013

Taking, on the 16th day of using it2-Dec-2013

Before use After 12 days

Before use After 4 days Before use After 6 days

No 。

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

KimKwonYangKwonRyuLeeSinKim

Choi

UhmGaLeeJangKwon

KwonKim

Ryu

Middle School teacher

High school teacher

Construction business owner

Import business owner

Housewives

Import business owner

Sports magazine team leader

College students

Elementary school students

CEO in shipping company

Housewives

CEO in consulting company

Director in advertising agency

Middle school students

Elementary school student

Section head in �Hyundai motors

Grandmother

Chronic fatigue, Knee ligament pain

Chronic fatigue

insomnia

wrist pain, both knee cartilages, scalp seborrheic dermatitis

Chronic fatigue, melasma, gastritis, slight coughing, tailbone pain

Chronic fatigue, neck psoriasis

neck disc pain

Atopy

Atopy

Chronic fatigue

Shingles, allergic rhinitis

Severe cold body aches for 3 weeks

Insomnia

Severe pimples

Slight pimples

High AST/ ALT levels and severe fatigue

Dyspepsia

Reduction of fatigue

Reduction of fatigue

Very significant improvement

Reduction of wrist pain

Reduction of fatigue, reduction of melasma, reduction of gastric pain

Reduction of fatigue, improvement of neck psoriasis

Reduction of pain

Improvement in one week

Improvement in one week

Reduction of fatigue

Complete recovery of Shingles, allergic rhinitis (2 weeks)

Recovery from a cold in 4 days

Smooth sleeping

Significant reduction of pimples, clean

Significant reduction of pimples, clean

Better bowel movement

Better digestion without taking digestive medicine

51

49

50

46

45

50

45

20

12

50

47

48

45

14

13

42

70

Name Age Occupation Physical condition before taking it Improvement effect After taking it

This report is the confidential document and property of the Ko rea Institute for Skin and Clinical Sciences;its transfer, plagiarization, and disclosure to thir parties is prohibited.

Results

No skin reaction was noticed at 30 minutes and 48 hours after removing patch

of Hydrolyzed DNA Cell Power Serum on the test area. Although ±irritation was

observed from one subject after 24 hours, the test material can be considered

as non irritant, regarding the mean score obtained was 0.17. No skin reaction

was noticed by the dermatologist at 30 minutes, 24 hours and 48 hours after

removing patch of Hydrolyzed DNA In-Deep Cell Power Mask on the test

area and the mean score obtained was 0.00. Thus, the test material can be

considered as non irritant. No skin reaction was noticed by the dermatologist

at 30 minutes, 24 hours and 48 hours after removing patch of Hydrolyzed DNA

Cell Power Mask on the test area and the mean score obtained was 0.00.

Thus, the test material can be considered as non irritant. No skin reaction was

noticed at 30 minutes and 24 hours after removing patch of ATO-DNA Power

Softener on the test area. Although ±irritation was observed from one subject

after 48 hours, the test material can be considered as non irritant, regarding the

mean score obtained was 0.17. No skin reaction was noticed at 30 minutes, 24

hours and 48 hours after removing patch of Hydrolyzed DNA Cell Power Skin

on the test area and the mean score obtained was 0.00. Thus, the test material

can be considered as non irritant. No skin reaction was noticed at 30 minutes,

24 hours and 48 hours after removing patch of ATO-DNA Wash & Shampoo

on the test area and the mean score obtained was 0.00. Thus, the test material

can be considered as non irritant. No skin reaction was noticed at 30 minutes,

24 hours and 48 hours after removing patch of PURE Bbee Soft Cleanser &

Shampoo on the test area and the mean score obtained was 0.00. Thus, the

test material can be considered as non irritant.

Conclusion

As the result of the safety test of ‘Hydrolyzed DNA Cell Power Lotion and

seventeen other kinds of cosmetics’, requested by TRUSMETICS CORPORATION,

all test materials can be considered as non irritant.

SUMMARY OF THE CLINICAL STUDY

Clinical Study Report

KISCS-CHC-ADE016

A safety test of 'Hydrolyzed DNA Cell Power

Lotion and seventeen other kinds of cosmetics’

after 24 hours patch on human skin

Requested by : TRUSMETICS CORPORATION (Korea)

Performed by : Korea Institute for Skin and Clinical Sciences ( Seoul, Korea)

Date : June 23, 2014

This report is the confidential document and property of the Korea Institute for Skin and Clinical Sciences;its transfer, plagiarization, and disclosure to thir parties is prohibited.

□Title of the clinical study : A safety test of ‘Hydrolyzed DNA Cell Power Lotion and seventeen

other kinds of cosmetics’ after 24 hours patch on human skin

□Case control No. : KISCS-CHC-ADE016

This study was conducted according to the regulations of designation as the test institution for

drugs, quasi-drugs, cosmetics, and medial devices; the guidelines of the management standards

for clinical drug evaluations; the guidelines of in vivo clinical and in vitro evaluation studies;

the guidelines of the experimental methods for cosmetic display and advertisements; and the

guidelines of the validation of functional cosmetics of the Ministry of Food and Drug Safety, Republic

of Korea; the laws of the bioethics and safety of the Ministry of Health and Welfare, Republic of

Korea; and the standard operation procedure of the Korea Institute for Skin and Clinical Sciences.

All procedures were investigated by the person in charge of reliability assurance.

Title of the

clinical study

A safety test of ‘Hydrolyzed DNA Cell Power Lotion and seventeen other kinds of

cosmetics’ after 24 hours patch on human skin

Date Step Categories Result Note

May 26, 2014 Study plan Reporting plan Approved

June 9, 2014 Performing clinical trial Reporting implementation Approved

June 16, 2014 Checking the data Raw data Approved

June 20, 2014 Inspection of draft report Inspecting draft report Approved Oral report

June 23, 2014 Inspection of final report Inspecting final report Approved

This report was prepared on the basis of the experiment results and accurately reflects the data.

June 23, 2014

Scientific director In Sook An, Ph.D. (seal)

Chief researcher Sungkwan An, Ph.D. (seal)

CERTIFICATE FOR RELIABILITY ASSURANCE

This report is the confidential document and property of the Ko rea Institute for Skin and Clinical Sciences;its transfer, plagiarization, and disclosure to thir parties is prohibited.

Title of the clinical study

A safety test of ‘Hydrolyzed DNA Cell Power Lotion and seventeen other kinds of cosmetics’ after 24 hours patch on human skin

Clinical trial institution

Korea Institute for Skin and Clinical Sciences3rd Floor, Life Science Building Annex, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 143-701, Republic of Korea

Trial periodMay 26, 2014 (study initiation) ~ June 23, 2014 (study termination)

For the study initiation, the person in charge of the study signed the clinical study proposal; for the study termination, the person in charge of the study signed the final report.

SubjectsThirty three subjects of either sex who met the inclusion criteria and were not included in

the exclusion criteria were selected for this study.

Name of the test materials

Hydrolyzed DNA Cell Power Lotion and seventeen other kinds of cosmetics

Methods

Thirty three healthy volunteers participated in the patch test using finn chamber

on skin. Each Finn Chambers containing 20 μL of a test material were fixed on

the upper back of the subjects after cleaning up the test area with 70% ethanol

and drying. In the case of a liquefied test material (#15-#18), 20 μL of a test

material was dropped into a filter paper disc fixed on 8 mm finn chamber. #17,

#18 were applied after diluted with sterile distilled water at 1% concentration. The

test area was assessed at 30 minutes, 24 hours and 48 hours after a single

application during 24 hours patch on the subjects. Skin reactions were scored by

a dermatology specialist, following the criterion of International Contact Dermatitis

Research Group (ICDRG).

Results

Requested by CHICCO Co., Ltd., skin reaction was assessed at 30 minutes, 24

hours and 48 hours after a single application during 24 hours patch of ‘Hydrolyzed

DNA Cell Power Lotion and the other kind of cosmetics’ on human skin. According

to the criterion of International Contact Dermatitis Research Group (ICDRG), mean

score was calculated after categorizing the degree of skin reaction. No skin reaction

was noticed by the dermatologist at 30 minutes, 24 hours and 48 hours after

removing patch of Hydrolyzed DNA Cell Power Lotion on the test area and the

mean score obtained was 0.00. Thus, the test material can be considered as non

irritant. No skin reaction was noticed at 30 minutes, 24 hours and 48 hours after

removing patch of PURE Bbee Intensive Care Cream on the test area and the mean

score obtained was 0.00. Thus, the test material can be considered as non irritant.

SUMMARY OF THE CLINICAL STUDY

NON-IRRITANT PRODUCTS PROVED BY KISCS

As Hydrolyzed DNA (low molecular weight DNA) is specially treated with enzymes and made in low molecular weight, itis the ingredient to improve the permeability of the moisturizing ingredient into the skin. With dual moisturizing power in inner and outer skins by combining hydrolyzed DNA and DNA-Na, the skin texture becomes smooth and the increase in the moisture contents of sebum is facilitated. Thus, it helps to improve the turnover of the skin and protect the skin from free radicas.

NUCLEO HYDROLYZED CELL POWER ANTI-AGING SOLUTION

NUCLEO HYDROLYZED CELL POWER ANTI-AGING SOLUTION

Hydrolyzed DNA regaining the beauty with the vitality by stopping the clock of aging.After we have studied the source of life, DNA, for a long time,

we have made the innovation of genetic dermatology for healthy skins.Beauty starts from healthy and powerful skins.

Containing hydrolyzed DNA which is the patented ingredient and which we study the basics of healthy skins with

Magical moisturizing and absorbing power of hydrolyzed DNA made in low olecular weight

Dual moisturizing powers filling and preventing with hydrolyzed DNA and DNA-Na

5 in 1 smart multi care line that can take care of the skin at once withthe addition of dual functional ingredients for anti-wrinkle and whitening

5in1

Whitening

MoisturizingHealthyGlow

Anti-WrinkleFirmness

Main Ingredient of NUCLEO CELL POWER LINE

Efficacy of the main common ingredients NUCLEO CELL POWER LINE

The secrets of DNA CELL POWER efficacy is “ HERE ”

Those 5 ingredients are commonly included in all of the CELL POWER line (toner, lotion, serum, eye cream, cream)Cleansing Foam includes only Hydrolyzed DNA, Sodium DNA and Pomegranate extract.

Adenosine <Functionality of wrinkle reduction> It is the nucleoside involved in signaling out of the cell and it is the raw material directly involved in growth, differentiation and homeostasis of cells. It is notified by KFDA as the raw material to improve the wrinkles.Efficacy _ raw material notified by KFDAIt has a very excellent would healing effect as the ingredient to promote the cell growth. In addition, it relieves the wrinkles and restores the elasticity by promoting the synthesis of collagen in the dermis.

Premium DNA Multi-Care Line to treat all your skin problems.(This is the result of our own evaluation conducted in June, 2014, with about 40 people)

Pomegranate extract Estrone and estradiol in pomegranate have the same structure of hormones secreted in the body compared to any botanical hormones extracted from other natural products. Thus, it is called phytoestrogen.Efficacy As phytoestrogens provide moisture and nutrition with the dermis by increasing the amount of collagen collective tissues which connect each cell, skin dryness and wrinkles are prevented and skins are made transparent, moist and elastic.

Niacinamide <Functionality of whitening> Niacinamide is a water soluble vitamin B3 directly involved in skin whitening.It is the nutrient and vitamin included in green vegetables as an essential ingredient in our body. Efficacy _ raw material notified by KFDAIf the skin is exposed to external conditions such as the sun light or stimulation, melanin pigment is moved in the skin and then blemishes appear in the skin.At this time, it plays a role to reduce the movement of melanin pigment.

NUCLEO HYDROLYZED DNA CELL POWER FOAM 150ml

SpecialtyGives glow and radiance with pomegranate which is similar with female hormoneContains Hydrolyzed DNA to keep skin cell healthy even from cleansing step Cleans off wastes not only inside of skin surface, but also inside of pores

Benefits Hypo-allergenic cleansing with soft bubbleNot dry & tight feels despite powerful cleansing effectGets a pure and transparent skin after washing the face

Cell Power`s specialty given to my skin

Felt the softened skin texture _98%

Felt firmer and lifted skin _98%

Got an instant glow quickly _97%

Felt their skin moisturized by the next morning _95%

The feeling of texture was very soft when applying it on the face _92%

Saw the visible radiance all over face _ 94%

Key Ingredients

No Added Ingredients

How to use

Hydrolyzed DNA, Sodium DNA, Pomegranate extracts

Paraben, Synthetic colorants, Methylated spirit, Mineral Oil, Triethanolamine, Sulfate-based surfactant

Paraben, Synthetic colorants, Methylated spirit, Mineral Oil, Triethanolamine, Sulfate-based surfactant

NUCLEO HYDROLYZED DNA CELL POWR SKIN 120ml

Specialty TrehalosMakes healthy skin barrier to stop the moisture from being evaporated with resurrection plant grown in the desert BetaineNature amino acid extracted from sugar beet is helpful in decreasing dryness and irritation, and obtain long-term hydra-tion Containing Hydration DNA Creates anti-wrinkles and brightening effect by delivering double functional ingredients (Adenosine, Naicinamide)Benefits Hypo-allergenic cleansing with soft bubbleNot dry & tight feels despite powerful cleansing effectGets a pure and transparent skin after washing the face

Key Ingredients

No Added Ingredients

How to use

Hydrolyzed DNA, Sodium DNA, Pomegranate extracts

Paraben, Synthetic colorants, Methylated spirit, Mineral Oil, Triethanolamine, Sulfate-based surfactant

Paraben, Synthetic colorants, Methylated spirit, Mineral Oil, Triethanolamine, Sulfate-based surfactant

Hydrolyzed DNA DNA-NaFormation of a moisturizing protective filmConditioning

Pomegranate extractsSoftening skin textureExfoliating

Activation of skin cells Double moisturizing

NUCLEO HYDROLYZED DNA CELL POWER SERUM 60ml

SpecialtyLinseed extracts Called an ancient life-giving seed, Linseed gives strong firmness and is very helpful in anti-aging containing enriched Omega-3 Fatty Acid, Dietary Fiber, and Lignan (called phytoestrogen)Containing Hydration DNA Gets glows and firmer skin by containing pomegranate extracts

Benefits Penetrates the skin speedily.Refines the facial line, and crafts radiant skin Soften the skin textureGets the beautiful glows from withinCreates firmer and illuminated skin with quick absorption

Key Ingredients

No Added Ingredients

How to use

Hydrolyzed DNA, SODIUM DNA, Pomegranate extract, Double functional ingredients , Anti-wrinkle ; adenosine, brightening ; Naicinamide Linseed extracts

Paraben, Synthetic colorants, Mineral Oil, Triethanolamine, Sulfate-based surfactant, Talc

After applying toner, pump two to three times on your palm and apply evenly over your entire face, moving outwards from the center.(Fist pump takes 5 or 10 times )

NUCLEO HYDROLYZED DNA CELL POWER Lotion 120ml

SpecialtyMacadamia nuts OilIt enhances firmness containing enriched Palmitoleic acid which is efficient in strong anti-oxidant Containing Hydration DNA Creates anti-wrinkles and brightening effect by delivering double functional ingredients (Adenosine, Naicinamide)Gets glows and firmer skin by containing pomegranate extracts

Benefits Gives enriched hydration and nourishment on exhausted skin Oil in water emulsion leaves the skin light and refreshed.Reduces the lines on the face and enhances the radianceSoftens the skin surface and balances oil and water inside of the skin.

Key Ingredients

No Added Ingredients

How to use

Hydrolyzed DNA, SODIUM DNA, Pomegranate extract, Double functional ingredients , Anti-wrinkle ; adenosine, brightening ; Naicinamide Macadamia nuts Oil

Propylene glycol, Paraben, Synthetic colorants, Methylated spirit,

Mineral Oil, Triethanolamine, Sulfate-based surfactant , Benzophenone, Tal

After applying serum, pump two to three times on your palm and apply evenly over your entire face, moving outwards from the center.(Fist pump takes 5 or 10 times )

NUCLEO HYDROLYZED DNA CELL POWER Eye Cream 35ml

SpecialtyShea butterProtects the hydration protective barrier from harmful external environment by filling the skin with nourishment Stop the skin cells from aging by containing Hydrolyzed DNA Creates anti-wrinkles and brightening effect by delivering double functional ingredients (Adenosine, Naicinamide)Gets glows and firmer skin by containing pomegranate extracts

Benefits Brightens the skin around the eyes, and Reduces the line on the face Enriched Hydrolyzed DNA penetrates the skin quickly Changes the tired eyes into vital and brightening eyes by plump-ing up areas around the eyes Gives firmness on the weak eye rim and rather sensitive skin around the eyes Highly-concentrated essential energy is delivered to the skin speedily

Key Ingredients

No Added Ingredients

How to use

Hydrolyzed DNA, SODIUM DNA, Pomegranate extract, Double functional ingredients , Anti-wrinkle ; adenosine, brightening ; Naicinamide, Shea Butter

Paraben, Synthetic colorants, Methylated spirit, Triethanolamine, Sulfate-based surfactant ,Talc

After applying lotion, Take a rice grain-sized amount and gently dot under the eyes. Using your ring finger, gently massage it on your skin in a circular motion..(Fist pump takes 5 or 10 times )

NUCLEO HYDROLYZED DNA CELL POWER Cream 50g

SpecialtyShea butterProtect the hydration protective barrier from harmful external environment by filling the skin with nourishment Stop the skin cells from aging by containing Hydrolyzed DNA Creates anti-wrinkles and brightening effect by delivering double functional ingredients (Adenosine, Naicinamide)Gets glows and firmer skin by containing pomegranate extracts

Benefits Refines the facial line and soften the skin texture. Gives visible firmness while applying it Enriched hydrolyzed DNA penetrates the skin quickly to make healthy skin and the cream adheres closely to skin while leaving skin feel soft and silky.Luxury multi-care cream to include double functional ingredi-ents for anti-wrinkle and brightening.

Key Ingredients

No Added Ingredients

How to use

Hydrolyzed DNA, SODIUM DNA, Pomegranate extract, Double functional ingredients , Anti-wrinkle ; adenosine, brightening ; Naicinamide, Shea Butter

Paraben, Synthetic colorants, Methylated spirit, Triethanolamine, Sulfate-based surfactant ,Talc

After applying eye cream, take a pearl-sized amount of the cream and dot your face. Massage gently outwards to spread evenly.

NUCLEO CELL POWER LINE

Cell Power`s specialty given to my skin

HYDROLYZED DNA IN DEEP DNA CELL POWERPREMIUM MASK PACK

3D mask with high quality which shines at any angleIt is a 3D mask with high quality to make new skins, since hydro-lyzed DNA which is a patented ingredient and dual functional ingredients for whitening, anti-wrinkles are fully added on a superfine fiber mask.

Charging the aged cells As regeneration of skin cells is maximized with hydrolyzed DNA ingredient and the production of collagen is promoted by increasing fibroblasts, young proteins for anti-aging are produced. It is a multi anti-aging mask to handle wrinkles, whitening, elasticity, luster and moisturizing which have been skin problems at once by providing skin cells with adequate nutrition.

Lost elasticity UP ! It is the hydrogel mask prepared for sagging V line and it is a child face mask to change the contour of the sagging face into a face with a clear and soft egg line.

Perfection of youthful-looking skin illuminating from any angle!

Enriched Hydrolyzed DNA is drenched in themicro-fiber mask sheets to adhere closely to the skin

and it gives the skin a healthy glow. This premium 3D mask is completed with separately added

hydrogel mask for the jaw-line to refine the facial profile through (called Egg line) lifting.

Step 1, 2 Anti- wrinkle, Whitening CarStep 3 Egg Line Care

3D EGG-LINEPREMIUM

CARE

Non-irritant product / Dermatologist Tested Double Effect / Double System

Microfiber Sheet 3D Egg Line Care

ALCOHOL DENAT. / TALCPARABEN / DYES

MICROFIBERSHEET

3DEGG-LINE CARE

3DCERTIFIED

ANTI-WRINKLEWHITENING(1,2 STEP)

DUAL

ANTI-WRINKLEWHITENING

ALCOHOL DENAT. / TALCPARABEN / DYES

100% COTTONSHEET

CERTIFIEDDUAL

DERMATOLOGISTTESTED

Comfort F

it

Comfort F

it

Comfort Fit

NUCLEO HYDROLYZED DNA CELL POWER MASK PACK

Double functions as anti-wrinkle agent and whitening; certified by the KFDA and contains Hydrolyzed DNA. 100% cotton mask is drenched with Hydrolyzed DNA to lift the sagging skin by supplying firmness and not only whitens the skin with Niacin Amide, but also reduces the wrinkles with Adenosine.

NUCELO SKIN VITALIZING CELL POWER HAND PACKㅣ钮卡落 细胞能量手膜 Ultra-Moisturizer Hand gloves contain energizing firming factors! Transforms hand skin from rough and wrinkled to soft and firm.

EGF & PEPTIDEGives firmness and healthy glow to the skin with collagen and skin activators which exist inside the body as a kind of Amino Acid.

NUCLEO SKIN VITALIZING CELL POWER FOOT PACKㅣ钮卡落 细胞能量足膜 The Easy Foot Care peels off the thick foot dead skin cells and fills the skin with the moisturizing factors. Cleans & Firms the thick and rough foot skin to make it transparent and vital.

EGF & PEPTIDEGives firmness and healthy glow to the skin with collagen and skin activators which exist inside the body as a kind of Amino Acid.

SPECIALLY FOR DRY SKINSENSITIVE SKINATOPY SKIN

Protection of skin health with Hydrolyzed DNA

Main Ingredient

Skin troubles will be resolved only if cells are healthy. Hydrolyzed DNA makes dry or damaged skins dense and smooth. As moisturizing film is formed by double moisturizing, skin is protected and healthy skins can be maintained.

Hydrolyzed DNAActivation of skin cells Double moisturizing

Wash & Shampoo Power Softener Power Lotion Power Cream Diaper Cream D-Line Cream

Chamomile flower extractDNA-NaFormation of a moisturizing protective filmConditioning

Increase in the amount of oxygen in body Improvement on elasticity

Skin elasticity Anti-inflammatory effect

Squalene

Cell Power`s specialty given to my skin

ATO-DNA WASH & SHAMPOO _ 190ml

SpecialtyChamomile has anti-bacterial and anti-inflammatory properties for skin. Rose oil : Soothes the skin and plump the skin with soft moisture power. Bergamot oil: Soothes the irritated skin and deliver the comfort to the troubled skin.Scented geranium oil : Sebum control and abundant moisturiz-ing power

Benefits As a cleanser not only for scalp and hair but for body, it gives abundant nourishment to the hair and body so hair and body keep moisturized even after washingCleans away skin and hair wastes made from external air pollution softly without stimulation The skin feels dry and tight even after washing speedily

Key Ingredients

No Added Ingredients

How to use

Hydrolyzed DNA, Chamomile oil, rose oil, Bergamot oil, Scented geranium oil

Paraben, Synthetic colorants, Synthetic fragrance, Methylated spirit, Mineral Oil

With a proper amount of the product, apply to wet body or scalp or hair to create a foamy lather, and rinse with water and enjoy.

ATO-DNA Power Softner _ 200ml

SpecialtyCreates double moisturizing power by delivering the rich nourishment to skin cells inside of skin, and coincidentally making skin protective barrierChamomile has anti-bacterial and anti-inflammatory properties for skin Rose oil : Sooth the skin and plump the skin with soft moisture power. Lavender has healing properties beneficial for wound skin while soothing the skin

Benefits Sooths the skin instantly with rich but fresh moisturizing power when applied.Cools and relaxes the overheated skin.Soften the rough skin texture.

Key Ingredients

No Added Ingredients

How to use

Hydrolyzed DNA , Sodium DNA, Chamomile oil, rose oil, Lavender oil

Paraben, Synthetic colorants, Synthetic fragrance, Methylated spirit, Mineral Oil

After cleansing foam, moisten a cotton pad with toner and apply evenly over the entire face. For better absorption, apply to the face in a patting motion.

ATO-DNA POWER LOTION _ 200ml

SpecialtyCreates double moisturizing power by delivering the rich nourishment to skin cells inside of skin, and coincidentally making skin protective barrier Safflower oil : Moisturizes the skin with abundant nourishment, and gives anti-oxidant propertiesOlive oil: Detoxifies the skin and gives anti-oxidant propertiesJasmine extract : Delivers the comfort to skin

Benefits Fills rich nourishment while balancing oil and water, and fresh hydration surrounding the skin completes healthy skin Relieves the dry and damaged skin and keeps the skin suppleCreates a protective skin barrier ensuring long lasting hydration.

Key Ingredients

No Added Ingredients

How to use

Hydrolyzed DNA ,Sodium DNA , Safflower oil , Olive oil, Jasmine extracts

Paraben, Synthetic colorants, Synthetic fragrance, Methylated spirit, Mineral Oil

Pump two to three times on your palm and apply evenly over your entire face, moving outwards from the center.

ATO-DNA Power Cream _ 80ml

SpecialtyCreates double moisturizing power by delivering the rich nourishment to skin cells inside of skin, and coincidentally making skin protective barrier Macadamia nuts OilIt enhances firmness containing enriched Palmitoleic acid which is efficient in strong anti-oxidant Mistletoe: Relieves the irritation while relaxing the skin Squalane: Enhances the skin protective barrierPortulaca: Gives comfort to skin while relaxing it.

Benefits Powerfully nourishing properties penetrate quickly into the skin leaving the silky texture. Leaves the skin fresh and light despite giving the rich nourish-ment.. Soothe the troubled skin instantly while providing long-lasting hydration .

Key Ingredients

No Added Ingredients

How to use

Hydrolyzed DNA , Sodium DNA,Macadamia nuts oil, Mistletoe, Squalane, Portulaca:

Paraben, Synthetic colorants, Synthetic fragrance,Methylated spirit, Mineral Oil

Take a pearl-sized amount of the cream and dot your face. Massage gently outwards to spread evenly.

ATO-DNA DIAPER CREAM _ 80ml

SpecialtyBergamot oil: Soothes the irritated skin and deliver the comfort to the troubled skinOlive oil: Detoxifies the skin and gives anti-oxidant propertiesLavender Oil : It has two effects to sterilize the skins and soothe them at the same time.

Benefits It soothes the skin trouble areas and you can feel a deep moisturizing effect.It makes delicate baby skins smooth and healthy.

Key Ingredients

No Added Ingredients

How to use

Hydrolyzed DNA , Sodium DNA, Bergamot oil, Olive oil, Lavender Oil

Mineral Oil, Benzophenone, Benzyl alcohol, Sulfate-based surfactant, Synthetic fragrance, Synthetic colorants, petrolatum, Paraben, Talc, Alcohol Denat.

When you change a baby diaper, you can gently apply and spread it out on the folded areas around the hip (hips, groin, neck, arms, legs, etc.) It would be good to sufficiently apply it on the affected skins after taking the bath.

NUCLEO D-LINE CREAM _ 200ml

SpecialtyMango Seed Butter : It delivers a sense of nutrition and a moisturizing effect of Mango seeds rich in nutrition.Shea Butter : Shea butter ingredients which had been used as medicinal ingredients in Africa mitigate skin damage.Coconut Oil : Since it is rapidly absorbed in the skins, it gives a deep moisturizing effect and promotes the absorption of other active ingredients.Macadamia Seed Oil : Since Macadamia Seed Oil has similar composition ingredients of sebum, it delivers the mitigation of skin damage and a moisturizing effect.

Benefits As it is gently absorbed after it is applied, it fills the essential energy for skin health.It prevents skin damage by soothing damaged skins such as chapped and sagging skins and giving a deep moisturizing effect.If is applied and spread out on the extremely dry skins, it softens skins and makes a smooth surface of skins.

Key Ingredients

No Added Ingredients

How to use

Hydrolyzed DNA , Sodium DNA, Mango Seed Butter, Shea Butter,Coconut Oil, Macadamia Seed Oil

Mineral Oil, Benzophenone, Benzyl alcohol, Sulfate-based surfactant, Synthetic fragrance, Synthetic colorants, petrolatum, Paraben, Talc, Alcohol Denat.

It is a formulation of concentrated cream, and it is thinly applied to the necessary area twice a day by giving a massage.

We make healthy cellby Providing Hydrolyzed DNA,Healthy Cell Brings Your Beauty & Happiness.

THANK YOU!

www.nucleo.co.krㅣ www.piccolini.krㅣwww.hxtru.co.kr ㅣwww.mddottore.com

TRUSMETICS CORPORATION 14TH FL JINYOUNG B/D, 560, EONJU-RO, GANGNAM-GU, SEOUL, KOREA T. 1599.5510 F. 02.6915.4550 E. [email protected]

DNACARESOLUTIONFORYOURBEAUTY &HEALTH


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