Clonación cell stem

http://www.abc.es/salud/noticias/celulas-piel-humanas-reprogramadas-convierten-14925.html

http://estaticos.elperiodico.com/resources/pdf/1/0/1368652108701.pdf

Somatic cell nuclear transfer

Align spindle and enucleatión pipette by rotating oocyte

Hold oocyte tightly make sure alignment by gently pushing the spindle with pipette tip laser assisted zona drilling

Gently insert pipette and aspirate spindles with….

small amount of cytoplast, as much as possible….

donor cell handling

Briefly soak donor cell into sev extract

donor cell pickup from sev extract

position spindle free oocyte

laser assisted zona drilling

injection donor cell into perivitelline aspace (NT)