Download - Analyte Quarterly 2012Q1
Immunoassays & MILLIPLEX® map KitsMultiplexing using the Luminex® technology
Analyte Quarterly Q1 2012Bone Metabolism • Cancer Biomarkers • Cardiovascular
Disease • Cell Signaling • Cellular Metabolism
Inflammation/Immunology • Metabolism
Neuroscience • Toxicity
EMD Millipore is a division of EMD KGaA, Darmstadt, Germany
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The Latest in MultiplexingDiscover the Latest in Innovative and Unique Assay Kits, Instruments and Services
MILLIPLEX® map Multiplex Immunoassay Kits
Magnetic Bead FormatKit Species Cat. No.
Adipocyte Human HADCYMAG-61K
Adipokine Panel 1 Human HADK1MAG-61K
Adipokine Panel 2 Human HADK2MAG-61K
Circulating Cancer Biomarker Panel 3
Human HCCBP3MAG-58K
High Sensitivity Cytokine Premix 13
Human HSCYPMX13-MAG
Th17 Human HTH17MAG-14K
Sepsis Panel 1 Human HSP1MAG-63K
Pyruvate Dehy-drogenas (PDH) Complex
Multi-species PDHMAG-13K
Fatty Acid Oxida-tion Panel 1
Human HFAO1MAG-11K
Fatty Acid Oxida-tion Panel 2
Human HFAO2MAG-11K
Oxidative Phos-phorylation
Human H0XPSMAG-16K
Neurological Dis-orders Panel 2
Human HND2MAG-39K
Akt/mTOR 11-Plex (Phospho)
Human 48-611MAG
Akt/mTOR 11-Plex (Total)
Human 48-612MAG
Heat Shock Protein Human 48-615MAG
EGFR Profiler Human 48-613MAG
EpiQuant Receptor Signaling Pathway
Human MPEQMAG-104K
EMD Millipore ELISAsKit Species Cat. No.
FGF-23 Rat EZRFGF23-42K
FGF-23 Mouse EZMFGF23-43K
MAGPIX® Instrument:• Multiplex analyte detection system
(Read more on page 19.)
Services:• SignalProfiler™ Services
(Read more on page 24.)
COMING SOON! EMD Millipore Cytokine ELISAs
NEW
PA
NEL
S
1
For Research Use Only.
See our website for Single Plex Kit catalogue numbers.
ELISA / R
IA
EMD Millipore provides an unparalleled level of
peer support—scientists working with scientists—
to provide you with the quality tools and services
you need to do your best work. With a wide
range of immunoassay kits, we advance your
research in many therapeutic areas including
metabolic disease (diabetes, obesity, cardio-
vascular), inflammation, immunology,
neuroscience, oncology and toxicity. These
include MILLIPLEX® map high content multiplex
biomarker panels utilizing the Luminex® xMAP®
technology platform. We offer the broadest
selection of ELISAs and RIAs as well as the
largest selection of multiplexing analytes and
applications of any Luminex® partner. Plus, all of
our kits are available in bulk packaging format.
Multiplex Kits & ELISAs
Kit Species Catalogue No.
a-Synuclein Human/ Mouse/Rat
NS400
Amyloid Beta, 1-40 Human EZHS40
Amyloid Beta, 1-42 Human EZHS42
Amyloid Beta, SET Human EZHS-SET
Amyloid Beta (Brain), 1-40
Human EZBRAIN40
Amyloid Beta (Brain), 1-42
Human EZBRAIN42
Amyloid Beta (Brain), SET
Human EZBRAIN-SET
Brain Derived Neurotrophic Factor (ChemiKine™)
Human/Rat CYT306
Kit Species Catalogue No.
IL-2 Human Coming Soon
IL-2 Mouse Coming Soon
IL-4 Human Coming Soon
IL-4 Mouse Coming Soon
IL-6 Human Coming Soon
IL-6 Mouse Coming Soon
IL-10 Human Coming Soon
IL-10 Mouse Coming Soon
TNFa Human Coming Soon
TNFa Mouse Coming Soon
Kit Species Catalogue No.
Epithelium Derived Factor (ChemiKine™)
Human CYT420
Glial fibrillary acidic protein (GFAP)
Human/ Mouse/Rat
NS830
Nerve Growth Factor (ChemiKine™)
Mouse/Rat CYT304
Neuropeptide S (NPS) Human EZHNPS-34K
Neuropeptide Y (NPY) Human EZHNPY-25K
Neuropeptide Y (NPY) Mouse/Rat EZRMNPY-27K
Nitrotyrosine Multi-species
17-376
OxyELISA® Oxidized Protein Quantitation
Multi-species
S7250
Phosphorylated Neurofilament (pNF-H)
Multi-species
NS170
S100B Human EZHS100B-33K
SAA-3 Mouse EZMSAA3-12K
sICAM-1 Human ECM335
sVCAM-1 Human ECM340
Neuroscience ELISAs
Cytokine ELISAs NEW!
millipore.com/analytes_Q1
ELISA - Cell-based AssaysDual Detect CELISAs™
Description Cat. No.
ErbB2 (Tyr1248) 17-490
H2A.X (Ser139) 17-720
2
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See our website for Single Plex Kit catalogue numbers.
Kit Species Catalogue No.
Laminin Human ECM310
Leptin “Dual Range” Human EZHL-80SK
Leptin Mouse EZML-82K
Leptin Rat EZRL-83K
Leptin Canine EZCL-31K
Nerve Growth Factor (ChemiKine™)
Mouse/Rat CYT304
Omentin-1 Human EZH0MNTN1-29K
Pancreatic Polypeptide
Human EZHPP40K
PEDF (ChemiKine™) Human CYT420
Procollagen Type IIA - N-Propeptide (PIIANP) †
Human EZPIIANP-53K
Proinsulin (Intact) Human EZHIPI-17K
Proinsulin (total) Human EZHPI-15K
PYY (total) Human EZHPYYT66K
RBP4 Human EZHRBP4-18K
Resistin Human EZHR-95K
sCD26 Human ECM345
sE-Selectin Human ECM330
sICAM-2 Human ECM346
sICAM-3 Human ECM337
sL-Selectin Human ECM331
ELIS
A /
RIA
ELISA - Circulating Biomarkers
Kit Species Catalogue No.
Adiponectin Human EZHADP-61K
Adiponectin Mouse EZMADP-60K
Adiponectin Rat EZRADP-62K
Adiponectin Canine EZCADP63K
Adiponectin – High Molecular Weight
Human EZHMWA-64K
Adipsin Mouse EZMADPSN-22
AGF (ANGPTL6) Human EZHAGF-28K
Amylin (active) • Human EZHA-52K
Amylin (total) • Human EZHAT-51K
Apo AIV Human EZHAP0A4-73K
Apo AV Human EZHAP0AV71K
BDNF Human/Rat CYT306
Chemerin Human EZHCMRN-57K
C-Peptide Human EZHCP-20K
C-Peptide 2 Rat/Mouse EZRMCP2-21K
CRP Human CYT298
CRP Rat CYT294
CRP Canine CYT296
FGF-21 Human EZHFGF21-19K
FGF-21 Rat/Mouse EZRMFGF21-26K
FGF-23 Human EZHFGF23-32K
FGF-23 Rat NEW! EZRFGF23-42K
FGF-23 Mouse NEW! EZMFGF23-43K
Fibronectin (Quantimatrix)
Human ECM300
Ghrelin (active) Human EZGRA-88K
Ghrelin (active) Rat/Mouse EZRGRA-90K
Ghrelin (total) Human EZGRT-89K
Ghrelin (total) Rat EZRGRT-91K
GIP (total) Human EZHGIP-54K
GIP (total) Rat EZRMGIP-55K
GLP-1 (active) • Multi-species
EGLP-35K
GLP-1 (total) Multi-species
EZGLP1T-36K
GLP-2 (total) Human/Rat EZGLP2-37K
Growth Hormone Rat/Mouse EZRMGH-45K
IFNa Human Cyt271
Insulin Human EZHI-14K
Insulin Rat/Mouse EZRMI-13K
Insulin (Animal Serum Free)
Human EZHIASF-14K
• Solid Plate Format † CU.S. Patent #: 5,541,066: 5,780,240
ELISA - Other Assays
DescriptionColorimetric
Catalogue No.Chemiluminescent
Catalogue No.
AP-1 Family 70-550 70-650
c-Fos 70-545 70-645
c-Jun 70-540 70-640
ErbB2 (Tyr1248) 17-490
HDAC 17-374
Jun/Fos 70-546 70-646
NFkB Family 70-560 70-660
NFkB p50 70-515 70-615
NFkB p50/p65 70-510 70-610
NFkB p65 70-520 70-620
PI3 Kinase Activity/Inhibitor
17-493
PhosphaFluor HTS OmniPhosphatase
17-448
Ras Activation 17-497
ssDNA Apoptosis APT225
Universal TF 70-501 70-601
EZ-Transcription Factor
3
For Research Use Only.
See our website for Single Plex Kit catalogue numbers.
ELISA / R
IA
Star ELISAs
ELISA - Cell Signaling / Intracellular
Description Catalogue No.
AKT1 (total) 17-455
EGFR (total) 17-460
Erk 1/2 (total) 17-463
FAK (total) 17-479
Insulin Receptor (b subunit) (total) 17-483
IkB (total) 17-485
JNK 1/2 (total) 17-465
MEK1 (total) 17-473
Met (total) 17-469
p38a (total) 17-487
p53 (total) 17-476
PRAS40 (total) 17-477
Src (total) 17-467
phospho-Akt (Ser473) 17-457
phospho-Akt (Thr308) 17-456
phospho-EGFR (Tyr1068) 17-462
phospho-EGFR (Tyr1173) 17-461
phospho-FAK (Tyr397) 17-480
phospho-IR (b Subunit) (Tyr 1162/1163) 17-484
phospho-IRS1 (Ser307 mouse/Ser312 Human)
17-459
phospho-IkB (Ser32) 17-486
phospho-JNK 1/2 (Thr183/Tyr185) 17-466
phospho-MEK1 (Ser218/222) 17-474
phospho-MET (Tyr1230/Tyr1234/Tyr1235) 17-470
phospho-p38a (Thr180/Tyr182) 17-488
phospho-p53 (Ser15) 17-475
phospho-PRAS40 (Thr246) 17-478
ELISA - Other Assays
Kit Species Catalogue No.
Fibronectin Human ECM300
sCD26 Human ECM345
sE-Selectin Human ECM330
sICAM-1 Human ECM335
sICAM-2 Human ECM331
sICAM-3 Human ECM337
sVCAM-1 Human ECM340
Extracellular Matrix
RIA
Kit Species Catalogue No.
Adiponectin Human HADP-61HK
Adiponectin Mouse MADP-60HK
C-Peptide Human HCP-20K
C-Peptide Rat RCP-21K
C-Peptide Canine CCP-24HK
C-Peptide Porcine PCP-22K
Ghrelin (active) Human GHRA-88HK
Ghrelin (total) Human GHRT-89HK
GLP-1 (active) Multi-species GLP1A-35HK
GLP-1 (total) Multi-species GLP1T-36HK
Glucagon Multi-species GL-32K
Insulin Rat RI-13K
Insulin Porcine PI-12K
Insulin Lispro LPI-16K
Insulin Specific Human HI-14K
Insulin Ultra Sensitive Human HI-11K
Insulin Rat SRI-13K
Leptin Human HL-81K
Leptin Mouse ML-82K
Leptin Rat RL-83K
Leptin Multi-species XL-85K
Proinsulin Human HPI-15K
PYY Rat/Mouse RMPYY-68HK
PYY (3-36) Specific Human PYY-67HK
PYY (total) Human PYYT-66HK
4
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MILLIPLEX® mapYour Source for Simultaneous Multi-Analyte Detection— the Voice of Luminex® xMAP® Technology
For R
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• The broadest selection of analytes across a wide range of disease states, including metabolic disease, inflammation, neurodegenerative disease, toxicity, cancer and more.
• All the components and reagents you need to detect multiple analytes simultaneously in a small sample size (10-50 µL)—all in a single kit, using a single catalogue number.
• Available in both magnetic and non-magnetic bead format. Magnetic polystyrene beads contain encapsulated magnetite with bead surface chemistries that are comparable to nonmagnetic beads. New magnetic bead panels will be introduced into our portfolio each quarter!
• Select a premixed kit or choose analytes within a panel to design a custom kit.
• Quality controls provided to qualify assay performance.
• Analytically validated panels that yield consistent analyte profiles irrespective of plex size.
• Standardized standard curve ranges across analytes and lots to ensure lot-to-lot consistency.
• Panels meet stringent manufacturing criteria to ensure batch-to-batch reproducibility.
• Environmentally friendly packaging: our boxes are 100% recyclable to reduce ecological footprints.
• Consolidated packaging available for larger orders to reduce storage needs.
MIL
LIPL
EX®
ma
p C
IRC
ULA
TIN
G
millipore.com/analytes_Q1
5
For Research Use Only.
MILLIPLEX
® ma
p C
IRCU
LATIN
G
Metabolism
Human Metabolic Hormone(Cat. No. HMHMAG-34K)
Amylin (active)†
Amylin (total)†
C-Peptide
Ghrelin (active) %
GIP (total)
GLP-1 (active)
Glucagon
IL-6
Insulin
Leptin
MCP-1
Pancreatic Polypeptide
PYY (total)
TNFa
Mouse Metabolic Hormone(Cat. No. MMHMAG-44K)
Amylin (active)
C-Peptide 2
Ghrelin (active) %
GIP (total)
GLP-1 (active)
Glucagon
IL-6
Insulin
Leptin
MCP-1
Pancreatic Polypeptide
PYY (total)
Resistin
TNFa
Rat Metabolic Hormone(Cat. No. RMHMAG-84K)
Amylin (active)
C-Peptide 2
Ghrelin (active) %
GIP (total)
GLP-1 (active)
Glucagon
IL-6
Insulin
Leptin
MCP-1
Pancreatic Polypeptide
PYY (total)
TNFa
Non-Human Primate Metabolic Hormone(Cat. No. NHPMHMAG-45K)
Amylin (active)
C-Peptide
Ghrelin (active) %
GIP (total)
GLP-1 (active)
Glucagon
IL-6
Insulin
Leptin
MCP-1
Pancreatic Polypeptide
PYY (total)
TNFa
Human Adipokine Panel ASerum/plasma samples(Cat. No. HADK1-61K-A) **
Adiponectin
PAI-1 (active)†
PAI-1 (total)†
Resistin
Human Adipokine Panel BSerum/plasma samples(Cat. No. HADK2-61K-B) **
HGF
IL-1bIL-6
IL-8
Insulin
Leptin
MCP-1
NGF
TNFa
Human Adipokine Panel 1Serum/Plasma Samples(Cat. No. HADK1MAG-61K) NEW!
Adiponectin
Adipsin
Lipocalin-2/NGAL
PAI-1 (total)
Resistin
Human Adipokine Panel 2Serum/Plasma Samples(Cat. No. HADK2MAG-61K) NEW!
Chemerin
HGF
IL-1bIL-6
IL-8
Insulin
Leptin
MCP-1
NGF
Omentin-1•
TNFa
Mouse AdipokineSerum/plasma samples(Cat. No. MADPK-71K)
IL-6
Insulin
Leptin
MCP-1
PAI-1 (total)
Resistin
TNFa
Rat AdipokineSerum/plasma samples(Cat. No. RADPK-81K)
IL-1bIL-6
Insulin
Leptin
MCP-1
PAI-1 (active)†
PAI-1 (total)†
TNFa
Human AdipocyteCell culture samples(Cat. No. HADCYT-61K) **
Adiponectin
HGF
IL-1bIL-6
IL-8
Leptin
MCP-1
NGF
PAI-1 (active)†
PAI-1 (total)†
Resistin
TNFa
Human AdipocyteCell Culture Samples(Cat. No. HADCYMAG-61K) NEW!
Adiponectin
HGF
IL-1bIL-6
IL-8
Leptin
MCP-1
NGF
PAI-1 (total)
Resistin
TNFa
Mouse AdipocyteCell culture samples(Cat. No. MADPCYT-72K)
Adiponectin
IL-6
Leptin
MCP-1
PAI-1 (total)
Resistin
TNFa
Rat AdipocyteCell culture samples(Cat. No. RADPCYT-82K)
Adiponectin
IL-1bIL-6
Leptin
MCP-1
PAI-1 (active)†
PAI-1 (total)†
TNFa
Human Liver Protein(Cat. No. HLPPMAG-57K)
AFP
ANGPTL3
ANGPTL4
ANGPTL6
FABP1
FGF-19
FGF-21
FGF-23
HGF
• Call for Availability
** Discontinued non-magnetic bead format March 31, 2012
% Requires a protease inhibitor during sample collection
Magnetic Bead Format
† Cannot be plexed together: 1) Amylin (active) and Amylin (total) 2) PAI-1 (active) and PAI-1 (total)
6
For R
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Only
.
See our website for Single Plex Kit catalogue numbers.
Human Pituitary Panel 2(Cat. No. HPTP2MAG-66K)
BDNF Prolactin
Non-Human Primate Pituitary Panel 1 (Cat. No. NHPPT1MG-46K)
ACTH
AGRP
CNTF
FSH
GH
LH
TSH
Non-Human Pituitary Panel 2(Cat. No. NHPPT2MG-46K
BDNF
Prolactin
Rat Pituitary (Cat. No. RPT-86K) **(Cat. No. RPTMAG-86K)
ACTH
BDNF
FSH
GH
LH
Prolactin
TSH
Canine Pituitary N(Cat. No. CPTMAG-96K)
ACTH
BDNF
FSH
GH
LH
Prolactin
TSH
Rat Stress Hormone(Cat. No. RSH69K)
ACTH
Corticosterone
Melatonin
Multi Species Steroid/ Thyroid Hormone(Cat. No. STTHMAG-21K) n
Cortisol
Estradiol
Progesterone
T3
T4
Testosterone
MIL
LIPL
EX®
ma
p C
IRC
ULA
TIN
G
Metabolism
Human Gut Hormone(Cat. No. HGT-68K)
Amylin (active)†
Amylin (total)†
Ghrelin (active) %
GIP (total)
GLP-1 (active)
Insulin
Leptin
Pancreatic Polypeptide
PYY (total)
Mouse Gut Hormone(Cat. No. MGT-78K)
Amylin (active)
Ghrelin (active) %
GIP (total)
GLP-1 (active)
Insulin
Leptin
Pancreatic Polypeptide
PYY (total)
Rat Gut Hormone(Cat. No. RGT-88K)
Amylin (active)
Ghrelin (active) %
GIP (total)
GLP-1 (active)
Insulin
Leptin
Pancreatic Polypeptide
PYY (total)
Canine Gut Hormone(Cat. No. CGT-98K) **(Cat. No. CGTMAG-98K)
Amylin (total)
Ghrelin (active) %
GIP (total)
GLP-1 (active)
Glucagon
Insulin
Leptin
Pancreatic Polypeptide
PYY (total)
Human Pituitary(Cat. No. HPT-66K) **
ACTH
FSH
GH
LH
Prolactin
TSH
Human Pituitary Panel 1 (Cat. No. HPTP1MAG-66K)
ACTH
AGRP
CNTF
FSH
GH
LH
TSH
Rat Thyroid Three Plex (Cat. No. RTHY-30K-03) ▼
T3
T4
TSH
Rat Thyroid Two Plex(Cat. No. RTHY-30K-T3T4) ▼
T3
T4
Rat Thyroid Single Plex(Cat. No. RTHY-30K-TSH)
TSH
Human IGF Binding Protein(Cat. No. HIGFBP-53K)
IGFBP1
IGFBP2
IGFBP3
IGFBP4
IGFBP5
IGFBP6
IGFBP7
Mouse IGF Binding Protein(Cat. No. MIGFBPMAG-43K)
IGFBP1
IGFBP2
IGFBP3
IGFBP5
IGFBP6
IGFBP7
Human Brain-Derived Protein(Cat. No. HBDP-33K) **
ACTH
AGRP
BDNF
CNTF
FSH
GH
LH
Prolactin
TSH
Single Plex KitsAdiponectin (Mouse)
Adiponectin (Rat)
IGF-1 (Mouse/Rat)
IGF-1 (Human)n
IGF-1 (Human)*n
IGF-2 (Human)*n
7
For Research Use Only.
See our website for Single Plex Kit catalogue numbers.
Bone Metabolism
Human Bone Panel 1ASerum/plasma samples(Cat. No. HBN1A-51K)
ACTH
Insulin
Leptin
OPG
Osteocalcin
Osteopontin
PTH
Human Bone Panel 1BTissue culture samples(Cat. No. HBN1B-51K)
ACTH
Adiponectin
IL-1b
IL-6
Insulin
Leptin
OPG
Osteocalcin
Osteopontin
PTH
TNFa
Mouse Bone Panel 1ASerum/plasma samples(Cat. No. MBN1A-41K)
ACTH
IL-6
Insulin
Leptin
OPG
TNFa
Mouse Bone Panel 1BTissue culture samples(Cat. No. MBN1B-41K)
ACTH
IL-6
Insulin
Leptin
OPG
Osteocalcin
TNFa
Mouse Bone Panel 2ASerum/plasma samples(Cat. No. MBN2A-41K)
ACTH
IL-6
Insulin
Leptin
RANKL
TNFa
Mouse Bone Panel 2BTissue culture samples(Cat. No. MBN2B-41K)
ACTH
IL-6
Insulin
Leptin
Osteocalcin
RANKL
TNFa
Rat Bone Panel 1(Cat. No. RBN1-31K)
ACTH
Insulin
Leptin
OPG
Rat Bone Panel 2(Cat. No. RBN2-31K)
ACTH
Insulin
Leptin
RANKL
Rat Bone Panel 3(Cat. No. RBN3-31K)
Osteocalcin
Osteopontin
PTH
Single Plex Bone KitsOPG (Mouse, Rat)
Osteocalcin (Mouse, Rat)
RANKL (Human, Mouse, Rat)
* Coming Soon
** Discontinued non-magnetic bead format March 31, 2011.
% Requires a protease inhibitor during sample collection
Magnetic Bead Format
† Cannot be plexed together: Amylin (active) and Amylin (total)
n Requires Sample Extraction
▼ Panel Premix Only
BON
E META
BOLISM
/ CV
DCardiovascular
Human CVD1(Cat. No. HCVD1-67AK)
Adiponectin
MMP-9
MPO
PAI-1 (total)
sE-Selectin
slCAM-1
sVCAM-1
Human CVD2(Acute phase proteins)(Cat. No. HCVD2-67BK)
CRP
Serum Amyloid A
Serum Amyloid P
Human CVD3(Cat. No. HCVD3-67CK)
IFNγ
IL-1b
IL-6
IL-8
IL-10
MCP-1
NT-proBNP
TNFa
VEGF
Human CVDPanel 1MB Format Coming Soon*
BNP
NT proBNP
CK-MB
CXCL6/GCP-2
CXCL16
sE-Selectin
Endocan-1 (ESM-1)
FABP3
FABP4
Follistatin (FST)
LIGHT
Oncostatin (OSM)
PAPP-A
sPECAM-1
Placental Growth Factor (PIGF)
Pentraxin-3 (PTX3)
Tissue Factor (TF)
Thrombomodulin
Troponin I (TnI)
Troponin T (TnT)
Human CVDPanel 2MB Format Coming Soon*
ADAMTS13
FABP5
GDF-15
D-dimer
sICAM-1
MPO
Myoglobin
Lipocalin-2/NGAL
Serum Amyloid A
sVCAM-1
sP-Selectin
8
For R
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.
See our website for Single Plex Kit catalogue numbers.
Human CVD Panel 3 (Acute Phase)MB Format Coming Soon*
a-2-Macroglobulin
Adipsin
AGP
GRP
Fetuin A
Fibrinogen
Haptoglobin
sL-Selectin
Platelet Factor 4 (PF4)
Serum Amyloid P
von Willebrand Factor
Human Apolipoproteins(Cat. No. APO-62K)
Apo AI
Apo AII
Apo B
Apo CII
Apo CIII
Apo E
Mouse CVD1(Cat. No. MCVD1-77AK)
MMP-9
PAI-1 (total)
sE-Selectin
slCAM-1
sVCAM-1
Mouse CVD2(Cat. No. MCVD277BK)
Adiponectin
Apo AI
Apo E
Fibrinogen
Mouse Acute Phase Panel 1 (Cat. No. MAP1MAG-76K)
Lipocalin-2/NGAL
Pentraxin-3 (PTX3)
Serum Amyloid A-3 (SAA-3)
CV
D /
CA
NC
ER Cancer BiomarkerCardiovascular
Human Circulating Cancer Biomarker Panel 1(Cat. No. HCCBP1MAG-58K)
AFP
CA125
CA15-3
CA19-9
CEA
CYFRA21-1
FGF2/bFGF
HCGbHE4
HGF
IL-6
IL-8
Leptin
MIF
Osteopontin
Prolactin
PSA (free)†
PSA (total)†
SCF
sFas
sFasL
TGFaTNFaTRAIL
VEGF
Human Circulating CancerBiomarker Panel 2(Cat. No. HCCBP2MAG-58K)
Antithrombin III
Coagulation factor XIII
Complement factor H (CFH)
Extracellular matrix protein 1 (ECM1)
Vitamin D binding protein
Vitronectin
Human Circulating Cancer Biomarker Panel 3Cat. No. HCCBP3MAG-58K) NEW!
Catalase
Cathepsin D
Ferritin
Fibroblast activation protein (FAP)
Galectin 3
IGFBP3
Melanoma inhibitory activity (MIA)
Myeloperoxidase (MPO)
Sex hormone binding globulin (SHBG)
* Coming Soon
** Discontinued non-magnetic bead format March 31, 2011.
Magnetic Bead Format
Expanded panel coming soon
† Cannot be plexed together: 1) PSA (free) and PSA (total)
Mouse Acute Phase Panel 2 (Cat. No. MAP2MAG-76K)
Adipsin
a-1-acid glycoprotein (AGP)
a-2-Macroglobulin
CRP
Haptoglobin
Serum Amyloid P
Pentraxin-2
Rat CVD1(Cat. No. RCVD1-89K)
BNP
IL-6
MCP-1
MPO
PAI-1
TIMP-1
TNFa
Troponin I
Troponin T
VEGF
von Willebrand Factor
Rat CVD2(Cat. No. RCVD2-89K)
sE-Selectin
sICAM-1
Single Plex CVD KitsCRP (Rat/Mouse)
Fibrinogen (Human)
Haptoglobin (Human)
See our website for Single Plex Kit catalogue numbers.
Human Angiogenesis/ Growth Factor(Cat. No. HAGP1MAG-12K)
Angiopoietin-2
BMP-9
EGF
Endoglin
Endothelin-1
FGF-1/FGF-acidic
FGF-2/FGF-basic
Follistatin
G-CSF
HB-EGF
HGF
IL-8
Leptin
PLGF
VEGF-A
VEGF-C
VEGF-D
9
For Research Use Only. C
AN
CER
/ NEU
ROSC
IENC
E / TOX
ICITY
Cancer Biomarker Neuroscience
Neuroscience
Human Neurodegenerative Disease Panel 1(Cat. No. HNDG1-36K) MB Format Coming Soon*
a-2-Macroglobulin
Apo AI
Apo CIII
Apo E
Complement C3
Complement Factor H
Prealbumin
Human Neurodegenerative Disease Panel 2(Cat. No. HNDG2-36K) MB Format Coming Soon*
a-1-Antitrypsin
Complement C4
CRP
MIP-4
PEDF
SAP
Human Neurodegenerative Disease Panel 3(Cat. No. HNDG3-36K) MB Format Coming Soon*
BDNF
Cathepsin D
MPO
PAI-1 (total)
PDGF-AA
PDGF-AB/BB
RANTES
sICAM-1
sNCAM
sVCAM-1
Toxicity
Human Neurodegenerative Disease Panel 4 CSF samples only (Cat. No. HNDG4-36K)
Ab1-40
Ab1-42
GDNF
S100B
sRAGE
Human Neurological Disorders Panel 1MB Format Coming Soon*
a-Synuclein
NAF-B
NSE
PARK5/UCHL1
PARK7/DJ1
Tau (Thr231)
Tau Total
Human Neurological Disorders Panel 2(Cat. No. HND2MAG-39K) NEW!
AGP
CP
HP
Serum Amyloid P
Human Neuropeptide n
(Cat. No. HNP-35K) a-MSH
b-Endorphin
Cortisol
Melatonin
Neurotensin
Orexin A
Oxytocin
Substance P
Human Kidney Toxicity Panel 1Serum samples(Cat. No. HKTX1MAG-38K)
KIM-1
Osteopontin
Renin
TFF-3
Human Kidney Toxicity Panel 2Serum samples(Cat. No. HKTX2MAG-38K)
b-2-Microglobulin
Clusterin
Cystatin C
Human Kidney Toxicity Panel 3Urine samples(Cat. No. HKTX3MAG-38K)
KIM-1
Renin
TFF-3
Human Kidney Toxicity Panel 4Urine samples(Cat. No. HKTX4MAG-38K)
Albumin
b-2-Microglobulin
Clusterin
Cystatin C
Osteopontin
Rat Kidney Toxicity Panel 1 (Cat. No. RKTX1-37K) MB Format Coming Soon* Clusterin
Osteopontin
KIM-1
Rat Kidney Toxicity Panel 2 (Cat. No. RKTX2-37K) MB Format Coming Soon* Albumin
b-2-Microglobulin
Cystatin C
10
For R
esea
rch
Use
Only
.
See our website for Single Plex Kit catalogue numbers.
INFL
AM
MA
TIO
N /
IM
MU
NO
LOG
Y Inflammation / Immunology
Human Cytokine/ Chemokine Panel I(Cat. No. MPXHCYTO-60K)(Cat. No. HCYTOMAG-60K)
(Cat. HCYTOMAG-60K-PX29) 29 (Cat. No. HCYTOMAG-60K-PX39) 39 (Cat. No. HCYTOMAG-60K-PX42) 42
EGF
Eotaxin
FGF-2
Flt-3 ligand
Fractalkine
G-CSF
GM-CSF
GRO
IFNa2
IFNγIL-1aIL-1bIL-1ra
IL-2
IL-3
IL-4
IL-5
IL-6
IL-7
IL-8
IL-9
IL-10
IL-12 (p40)
IL-12 (p70)
IL-13
IL-15
IL-17A
IP-10
MCP-1/CCL2/MCAF
MCP-3/CCL7
MDC/CCL22
MIP-1aMIP-1bPDGF-AA†
PDGF-AB/BB†
RANTES†
sIL-2RasCD40L
TGFaTNFaTNFbVEGF
Human Cytokine/ Chemokine Panel II(Cat. No. MPXHCYP2-62K)(Cat. No. HCYP2MAG-62K) (Cat. No. HCP2MAG-62K-PX23) 23
6Ckine/CCL21
BCA-1/CXCL13
CTACK/CCL27
ENA-78/CXCL5
Eotaxin-2/CCL24/MPIF-2
Eotaxin-3/CCL26
IL-16
IL-20
IL-21
IL-23
IL-28a
IL-33/NF-HEV (mature)
I-309/CCL1
LIF
MCP-2
MCP-4
MIP-1d (MIP-5/CCL15)
SCF
SDF-1a+b/CXCL12
TARC/CCL17
TPO
TRAIL/TNFSF10
TSLP
Human Cytokine/ Chemokine Panel III(Cat. No. MPXHCYP3-63K)(Cat. No. HCYP3MAG-63K) (Cat. No. HCP3MAG-63K-PX9) 9
(Cat. No. HCP3MAG-63K-PX11) 11
HCC-1/CCL14a†
IL-11
IL-29/IFNγ2
I-TAC/CXCL11
LIX/CXCL6/GCP-2
Lymphotactin
M-CSF
MIG/CXCL9
MIP-3a/CCL20
MIP-3b/CCL19
NAP-2/CXCL7†
* Coming Soon
Magnetic Bead Format
** Discontinued non-magnetic bead format March 31, 2012
Expanded panel coming soon
# Magnetic Premix Panel
† Cannot be plexed together: 1) PDGF-AA. PDGF-AB/BB and RANTES 2) HCC-1/CCL14a and NAP-2/CXCL7
Human High Sensitivity Cytokine/Chemokine(Cat. No. HSCYTO-60SK)(Cat. No. HSCYTMAG-60SK) (Cat. No. HSCYPMX13-MAG) 13 NEW!
GM-CSF
IL-1bIL-2
IL-4
IL-5
IL-6
IL-7
IL-8
IL-10
IL-12 (p70)
IL-13
IFNγTNFa
Human Soluble Cytokine Recepter(Cat. No. HSCR-32K)
sCD30
sEGFR
sgp130
sIL-1RI
sIL-1RII
sIL-2RasIL-4R
sIL-6R
sRAGE
sTNF RI
sTNF RII
sVEGF R1
sVEGF R2
sVEGF R3
Human Th17(Cat. No. HTH17MAG-14K) NEW!
GM-CSF
IFNγIL-1bIL-2
IL-4
IL-5
IL-6
IL-9
IL-10
IL-12 (p70)
IL-13
IL-15
IL-17A
IL-17E
IL-17F
IL-21
IL-22
IL-23
IL-27
IL-28A
IL-31
IL-33
MIP-3aTNFaTNFb
Human CD8MB Format Coming Soon*
sCD137
sFas
sFasL
GM-CSF
Granzyme A
Granzyme B
IFNγIL-2
IL-4
IL-5
IL-6
IL-10
IL-13
MIP-1aMIP-1bPerforin
TNFa
11
For Research Use Only.
See our website for Single Plex Kit catalogue numbers.
INFLA
MM
ATIO
N / IM
MU
NO
LOG
YInflammation / Immunology
Mouse Cytokine/ Chemokine Panel II(Cat. No. MPXMCYP2-73K)(Cat. No. MCYP2MAG-73K) (Cat. MCP2MG-73K-PX12) 12
EPO
Exodus-2
Fractalkine
IL-16
IL-21
IL-22
IL-25/IL-17E
IL-28B
MCP-5
MIP-3aMIP-3bTARC
Mouse Cytokine/ Chemokine Panel III(Cat. No. MPXMCYP3-74K)(Cat. No. MCYP3MAG-74K) (Cat. MCP3MAG-74K-PX6) 6
IL-20
IL-23
IL-27
IL-33
MDC
TIMP-1
Mouse Soluble Cytokine Receptor(Cat. No. MSCR-42K)
sCD30
sgp130
sIL-1RI
sIL-1RII
sIL-2RasIL-4R
sIL-6R
sRAGE
sTNF RI
sTNF RII
sVEGF R1
sVEGF R2
sVEGF R3
Mouse Th17 MB Format Coming Soon*
GM-CSF
IFNγIL-1bIL-2
IL-4
IL-5
IL-6
IL-10
IL-12 (p70)
IL-13
IL-15
IL-17A
IL-17E
IL-17F
IL-21
IL-22
IL-23
IL-27
IL-28A
IL-28B
IL-31
IL-33
MIP-3aTNFaTNFb
Human TIMP 1Serum samples(Cat. No. HTIMP1-54K)
TIMP-1
TIMP-2
Human TIMP 2Cell culture samples(Cat. No. HTIMP2-54K)
TIMP-1
TIMP-2
TIMP-3
TIMP-4
Human Sepsis Panel 1(Cat. No. HSEP-63K) ** (Cat. No. HSP1MAG-63K) NEW!
MIF
PAI-1 (total)
sFas
sFasL
sICAM-1
sVCAM-1
Human Sepsis Panel 2 (Cat. No. HSP2MAG-63K)
Granzyme B
HSP70
IL-1aIL-8
MIP-1aMIP-1bMMP-8
Human Sepsis Panel 3 (Cat. No. HSP3MAG-63K)
Lactotransferrin (LTF)
Lipocalin-1
Neutrophil Elastase-2 (ELA2)
Resistin
Thrombospondin-1 (TSP-1)
Non-Human Primate Cytokine/Chemokine(Cat. No. MPXPRCYTO-40K)(Cat. No. PRCYTOMAG-40K) (Cat. PCYTMG-40K-PX23) 23
G-CSF
GM-CSF
IL-1bIL-1ra
IL-2
IL-4
IL-5
IL-6
IL-8
IL-10
IL-12/23 (p40)
IL-13
IL-15
IL-17A
IL-18
IFNγMCP-1/CCL2/MCAF
MIP-1aMIP-1bsCD40L
TGFaTNFaVEGF
Mouse Cytokine/ Chemokine Panel I (Cat. No. MPXMCYTO-70K)(Cat. No. MCYTOMAG-70K) (Cat. No. MCYTOMAG-70K-PMX) 25
(Cat. No. MCYTMG-70K-PX32) 32
Eotaxin
G-CSF
GM-CSF
IL-1aIL-1bIL-2
IL-3
IL-4
IL-5
IL-6
IL-7
IL-9
IL-10
IL-12 (p40)
IL-12 (p70)
IL-13
IL-15
IL-17A
IFNγIP-10
KC
LIF
LIX
MCP-1/CCL2/MCAF
M-CSF
MIG
MIP-1aMIP-1bMIP-2
RANTES
TNFaVEGF
Human MMP 1(Cat. No. HMMP1-55K)
MMP-3
MMP-12
MMP-13
Human MMP 2(Cat. No. HMMP2-55K)
MMP-1
MMP-2
MMP-7
MMP-9
MMP-10
12
For R
esea
rch
Use
Only
.
See our website for Single Plex Kit catalogue numbers.
INFL
AM
MA
TIO
N /
IM
MU
NO
LOG
Y /
SKIN
/ I
SOTY
PIN
G Inflammation & ImmunologyMouse CD8 MB Format Coming Soon*
sCD137
sFas
sFasL
GM-CSF
Granzyme B
IFNγIL-2
IL-4
IL-5
IL-6
IL-10
IL-13
MIP-1aMIP-1bRANTES
TNFa
Rat Cytokine/Chemokine(Cat. No. RCYTO-80K) **(Cat. No. RCYTOMAG-80K) (Cat. No. RCYTOMAG-80K-PMX) 23
Eotaxin
G-CSF
GM-CSF
GRO/KC
IFNγIL-1aIL-1bIL-2
IL-4
IL-5
IL-6
IL-10
IL-12 (p70)
IL-13
IL-17A
IL-18
IP-10
Leptin
MCP-1/CCL2/MCAF
MIP-1aRANTES
TNFaVEGF
Canine Cytokine/Chemokine(Cat. No. CCYTOMAG-90K) (Cat. No. CCYTMG-90K-PX13) 13
GM-CSF
IFNγIL-2
IL-6
IL-7
IL-8
IL-10
IL-15
IL-18
IP-10
KC-like
MCP-1/CCL2/MCAF
TNFa
Multi-species TGFb 1-plex(Cat. No. TGFB-64K-01)
TGFb1
Multi-species TGFb 3-plex ▼(Cat. No. TGFB-64K-03)
TGFb1
TGFb2
TGFb3
SkinHuman Skin(Cat. No. SKIN-50K)
Cortisol
Fibronectin
Human Serum Albumin (HSA)
Involucrin
Keratin-6
Keratin-1,10
LPS
IsotypingHuman Immunoglobulin Isotyping(Cat. No. HGAM-301) ** ▼(Cat. No. HGAMMAG-301K)
IgA
IgG1
IgG2
IgG3
IgG4
IgM
Mouse Immunoglobulin Isotyping (Cat. No. MGAMMAG-300K)
IgA
IgG1
IgG2a
IgG2b
IgG3
IgM
Rat Immunoglobulin Isotyping(Cat. No. RGAMMAG-302K)
IgG1
IgG2a
IgG2b
IgG2c
IgA
IgE
IgM
Single Plex IgE
IgE (Human)
IgE (Mouse)
* Coming Soon
** Discontinued non-magnetic bead format March 31, 2012
Magnetic Bead Format
Expanded panel coming soon
# Magnetic Premix Panel
° Call for availability in the magnetic bead panel.
▼ Panel Premix Only
13
For Research Use Only.
Cell Signaling & Phosphoprotein DetectionNew Challenges, Faster Answers
Multiplexing, using the Luminex® xMAP®
platform, provides faster answers to cell signaling
questions compared to traditional Western blots,
mass spectrometry analysis, and radioactive
phosphorylation assays that require large
amounts of sample.
EMD Millipore is proud to provide you with three
MILLIPLEX® Map cell signaling portfolios based on
the Luminex® xMAP® platform.
• MILLIPLEX® map Cellular Metabolism Assays
• MILLIPLEX® map Cell Signaling Assays
• MILLIPLEX® map EpiQuant™ Cell Signaling Assays
Cellular Metabolism AssaysIntroducing EMD Millipore's newest product
portfolio for the Luminex® xMAP® platform,
Cellular Metabolism. These panels will enable
you to:
• Measure analytes within the panels in small
sample sizes of cell lysate or tissue extract
• Examine intracellular pathways regulating
cellular metabolism and energy production
• Facilitate research areas such as mitochondrial
dysfunction and toxicity, metabolic disorders,
neurological disorders, cancer and aging
millipore.com/analytes_Q1
MILLIPLEX® map Traditional Cell Signaling AssaysOur traditional cell signaling kits and
MAPmates™ offer you the ability to:
• Measure multiple total or phosphorylated
proteins along a signaling pathway, in a single
sample
• Simultaneously measure sites in multiple
pathways
• Conduct all necessary measurements using
minimal sample volume
• Choose from analytically validated kits or
design your custom assay with our MAPmates™
MILLIPLEX® map EpiQuant™ AssaysIntroducing EpiQuant™ technology as part of the
MILLIPLEX® Map portfolio. The union of two
powerful technologies—EMD Millipore’s EpiQuant™
technology and the Luminex® xMAP® platform—
provides you with the most advanced quantitative
cell signaling assays.
Answering the challenges of multiplex cell signaling,
MILLIPLEX® Map EpiQuant™ panels enable you to:
• Obtain quantitative results at picomolar
sensitivities
• Measure phosphorylation concentrations of
multiple sites on the same protein all in one well
• Measure both total and phospho-protein—
all in the same well
• Customize your assay by selecting analytes
you want to study
• Design small- or large-plexed panels with ease
MILLIPLEX
® ma
p C
ELL SIGN
ALIN
G
14
For R
esea
rch
Use
Only
.
See our website for Single Plex Kit catalogue numbers.
MIL
LIPL
EX®
ma
p CE
LLU
LAR
MET
ABO
LISM
Traditional Cell SignalingHuman Multi-Pathway - 8 Plex(Cat. No. 48-680) ▼
MB Format Coming Soon*
Analyte Total Phosphorylated
CREB 3 (Ser133)
Erk/MAPK 1/2 3 (Thr185/Tyr187)
IkBa 3 (Ser32)
JNK 3 (Thr183/Tyr185)
p38 3 (Thr180/Tyr182)
p70 S6 Kinase 3 (Thr412)
STAT3 3 (Ser727)
STAT5A/B 3 (Tyr694/699)
Human T Cell Receptor - 7 Plex(Cat. No. 48-690) ▼
Analyte Total Phosphorylated
CD3e 3 (pan Tyr)
CREB 3 (Ser133)
Erk/MAPK 1/2 3 (Thr185/Tyr187)
LAT 3 (pan Tyr)
Lck 3 (pan Tyr)
Syk 3 (pan Tyr)
ZAP-70 3 (pan Tyr)
Human Apoptosis - 3 Plex(Cat. No. 48-670) ▼
MB Format Coming Soon* Analyte Total Phosphorylated
Active Caspase 3 3
Active Cleaved PARP 3
GAPDH 3
Cellular Metabolism Assays
Human Drug MetabolismMB Format Coming Soon*
ALDH4A1
CES1
EPHX1
MAO-A
MAO-B
* Coming Soon
Magnetic Bead Format
Expanded panel coming soon
▼ Panel Only Premix
Multi-species Pyruvate Dehydrogenase (PDH) Complex ▼
(Cat. No. PDHMAG-13K) NEW!
Analyte Total Phosphorylated
PDH 3
PDH (Ser232)
PDH (Ser293)
PDH (Ser300)
Human Fatty Acid Oxidation Panel 1 (Cat. No. HFAO1MAG-11K) NEW!
Acetyl-CoA acyltransferase 2 (ACAA2)
Peroxisomal bifunctional enzyme (LPBE/ECHD)
Short-chain 3-hydroxyacyl-CoA dehydrogenase (SCHAD)
Mitochondrial trifunctional protein (TFP)
Human Fatty Acid Oxidation Panel 2 (Cat. No. HFAO2MAG-11K) NEW! Carnitine palmitoyltransferase 2 (CPT2)
2,4-dienoyl-CoA reductase (DECR1)
Electron transfer flavoprotein, mitochondrial (ETF)
Medium-chain acyl-CoA dehydrogenase (MCAD)
Peroxisomal multifunctional enzyme 2 (MFE2)
Human Oxidative Phosphorylation(Cat. No. H0XPSMAG-16K) NEW!
Complex I (NADH-Ubiquinone Oxidoreductase)
Complex II (Succinate Ubiquinone Oxidoreductase)
Complex III (Ubiquinone Cytochrome C Oxidoreductase)
Complex IV (Cytochrome C Oxidase)
Complex V (ATP Synthase)
NNT (Nicotinamide Nucleotide Transhydrogenase)
Rat/Mouse Oxidative Phosphorylation ▼
MB Format Coming Soon* Complex I (NADH-Ubiquinone Oxidoreductase)
Complex III (Ubiquinone Cytochrome C Oxidoreductase)
Complex V (ATP Synthase)
Human Oxidative StressMB Format Coming Soon* Catalase
GPX1
PRX2 (PRDX2)
SOD1
SOD2
TRX1
15
For Research Use Only.
See our website for Single Plex Kit catalogue numbers.
Traditional Cell SignalingTR
AD
ITION
AL C
ELL SIGN
ALIN
G
Human MAPK/SAPK - 10 Plex(Cat. No. 48-660) ▼
Analyte Total Phosphorylated
ATF2 3 (Thr69/71)
ErK/MAPK 1/2 3 (Thr185/Tyr187)
HSP27 3 (Ser78)
JNK 3 (Thr183/Tyr185)
c-Jun 3 (Ser73)
MEK1 3 (Ser222)
MSK1 3 (Ser212)
p38 (SAPK2A/B) 3 (Thr180/Tyr182)
p53 3 (Ser15)
STAT1 3 (Tyr707)
Human SRC Family Kinase - 8 Plex(Cat. No. 48-650) ▼
Analyte Total Phosphorylated
Blk 3 (pan Tyr)
Fgr 3 (pan Tyr)
Fyn 3 (pan Tyr)
Hck 3 (pan Tyr)
Lck 3 (pan Tyr)
Lyn 3 (pan Tyr)
Src 3 (pan Tyr)
Yes 3 (pan Tyr)
Human Akt / mTOR - 11 Plex(Cat. No. 48-611) ▼
(Cat. No. 48-611MAG) ▼ NEW! Analyte Total Phosphorylated
Akt 3 (Ser473)
GSK3a 3 (Ser21)
GSK3b 3 (Ser9)
IGF1R 3 (Tyr1135/1136)
IR 3 (Tyr1162/1163)
IRS1 3 (Ser312)
mTOR 3 (Ser2448)
p70 S6 Kinase 3 (Thr412)
PTEN 3 (Ser380)
RPS6 3 (Ser235/236)
TSC2 3 (Ser939)
Human Total Akt/mTOR - 11 Plex(Cat. No. 48-612MAG) ▼ NEW! Analyte Total Phosphorylated
Akt 3
GSK3a 3
GSK3b 3
IGF1R 3
IR 3
IRS1 3
mTOR 3
p70S6 Kinase 3
PTEN 3
RPS6 3
TSC2 3
Human Angiogenesis RTK - 10 PlexMB Format Coming Soon* Analyte Total Phosphorylated
c-Kit 3 pan Tyr
Flt3 3 pan Tyr
M-CSFR 3 pan Tyr
PDGFRa 3 pan Tyr
PDGFRb 3 pan Tyr
Tie1 3 pan Tyr
Tie2 3 pan Tyr
VEGF R1 3 pan Tyr
VEGF R2 3 pan Tyr
VEGF R3 3 pan Tyr
Human Total Angiogenesis RTK - 10 PlexMB Format Coming Soon*
Analyte Total Phosphorylated
c-Kit 3
Flt3 3
M-CSFR 3
PDGFRa 3
PDGFRb 3
Tie1 3
Tie2 3
VEGF R1 3
VEGF R2 3
VEGF R3 3
16
For R
esea
rch
Use
Only
.
See our website for Single Plex Kit catalogue numbers.
Human Mitogenesis RTK – 8 PlexMB Format Coming Soon*
Analyte Total Phosphorylated
cMet/HGFR 3 pan Tyr
EGFR 3 pan Tyr
ErbB2/HER2 3 pan Tyr
ErbB3 3 pan Tyr
ErbB4 3 pan Tyr
IGF1R 3 pan Tyr
IR 3 pan Tyr
Human Total Mitogenesis RTK - 8 PlexMB Format Coming Soon*
Analyte Total Phosphorylated
cMet/HGFR 3
EGFR 3
ErbB2/HER2 3
ErbB3 3
ErbB4 3
IGF1R 3
IR 3
Human Heat Shock Protein – 5 Plex(Cat. No. 48-615MAG) NEW! Analyte Total Phosphorylated
HSP27 3
Phospho-HSP27 3 (Ser78/Ser82)
HSP60 3
HSP70 3
HSP90a 3
Human EGFR Profiler - 9 Plex(Cat. No. 48-613MAG) NEW! Analyte Total Phosphorylated
EGFR 3
EGFR 3 (Thr654)
EGFR 3 (Thr669)
EGFR 3 (Tyr845)
EGFR 3 (Tyr1045)
EGFR 3 (Ser1047)
EGFR 3 (Tyr1068)
EGFR 3 (Tyr1086)
TRA
DIT
ION
AL
CEL
L SI
GN
ALI
NG
The following MAPmates™ should not be plexed together:
• MAPmates™ that require a different assay buffer
• Phosphospecific and total MAPmate™ pairs, e.g. total GSK3b and GSK3b (Ser 9)
• Pan Tyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and HSP27 (Ser78)
• More than 1 phospho-MAPmate™ for a single target
(Akt, STAT3, p53, Src, Rb)
Buffer & Detection Kit (Cat. No. 48-602)Required to run all MAPmate™ assays.
MAPmates™Analyte Total Phosphorylated
Akt/PKB (assay buffer 1) 3 3 (Ser473)
Akt/PKB (assay buffer 1) 3 (Thr308)
Akt/PKB (assay buffer 2) 3 3 (Ser473)
ATF2 3 3 (Thr69/71)
BAD 3 3 (Ser112)
Active Caspase 3 3
CREB 3 3 (Ser133)
EGF-Receptor 3 3 (PanTyr)
Erb-B2/HER2 3 (PanTyr)
Erk/MAPK 1/2 3 3 (Thr185/Tyr187)
Human STAT - 5 Plex(Cat. No. 48-610) ▼
MB Format Coming Soon*
Analyte Total Phosphorylated
STAT1 3 (Tyr701)
STAT2 3 (Tyr690)
STAT3 3 (Tyr705)
STAT5A/B 3 (Tyr694/699)
STAT6 3 (Tyr641)
Human TGFb – 6 PlexMB Format Coming Soon*
Analyte Total Phosphorylated
Akt 3 (Ser473)
Erk 3 (Thr185/Tyr187)
SMAD2 3 (Ser465/467)
SMAD3 3 (Ser423/425)
SMAD4 3
TGFbRII 3
Traditional Cell Signaling
17
For Research Use Only.
See our website for Single Plex Kit catalogue numbers.
MILLIPLEX
® ma
p EPIQU
AN
T™
Analyte Total Phosphorylated
GSK3b 3 3 (Ser9)
H2A.X 3 (Ser139)
HIF-1a 3
HSP27 3 3 (Ser78)
IGF1R 3 3 (PanTyr)
IkBa 3 3 (Ser32)
IR 3 3 (Tyr1162/1163)
IRS1 3 3 (PanTyr)
JNK 3 3 (Thr183/Tyr185)
c-Jun 3 (Ser73)
c-Kit 3 3 (PanTyr)
Lck 3 3 (PanTyr)
Lck 3 (Tyr394)
Lyn 3 3 (Tyr397)
c-Met/HGFR 3 3 (PanTyr)
MEK1 3 3 (Ser222)
mTOR 3 3 (Ser2448)
NFkB 3 3 (Ser536)
p21 3
p38 (SAPK2A/B) 3 3 (Thr180/Tyr182)
p53 3 3 (Ser15)
p53 3 (Ser46)
p70 S6 Kinase 3 3 (Thr412)
Cleaved PARP 3
PDGF Receptor a 3 (PanTyr)
PDGF Receptor b 3 (PanTyr, mouse)
PRAS40 3 3 (Thr246)
PTEN 3 3 (Ser380)
Rb 3 3 (Thr826)
Rb 3 (Thr252)
Rb 3 (Ser811)
Src 3 3 (Tyr419)
Src 3 (Tyr530)
STAT1 3 3 (Tyr701)
STAT2 3 3 (Tyr690)
STAT3 3 3 (Tyr705)
STAT3 3 (Ser727)
STAT5A/B 3 (Tyr694/699)
STAT6 3 (Tyr641)
ZAP-70 3 (PanTyr)
GAPDH 3 (Human)
MILLIPLEX® map EpiQuant™
EGFR Signaling Pathway Panel(Cat. No. MPEQMAG-110K)
CSK (Tyr304)
EGFR/HER1 Total
EGFR/HER1 (Tyr1069/1092)
EGFR/HER1 (Tyr1110/1125)
ErbB2/HER2 (Tyr1023)
ErbB3/HER3(Tyr1197/1199/1262/1276/ 1298/1307)
ERK 1/2 (Tyr204/187)
FAK (Tyr397/407)
FAK (Tyr861)
FRS2 (Tyr436)
Gab1 (Tyr285/307/317)
Gab2 (Tyr266)
Gab2 (Tyr584)
Gab2 (Tyr614)
PI3Kp110δ (Tyr485)
PKCµ (Tyr502)
PLCγ1 (Tyr1253)
PLCγ2 (Tyr1192-1217)
Shc (Tyr349/350)
SPRY2 (Tyr55)
STAT3 (Tyr705)
TAFII68 Total (loading control)
Human Receptor Tyrosine Kinase Signaling Pathway(Cat. No. MPEQMAG-104K) NEW! EGFR2/HER2 Total
EGFR2/HER2 (Tyr1023)
EGFR2/HER2 (Tyr1112/1139)
EGFR2/HER2 (Tyr 1196/1221/1222)
EGFR3/Erb-B3 Total
EGFR3/Erb-B3 (Tyr1197/1199/ 1262/1276/1298/1307)
Kit Total
Kit/SCFR (Tyr545)
Kit/SCFR (Tyr936)
Lyn Total
Lyn (Tyr397)
PDGFRa Total
PDGFRa (Tyr720/730)
PI3K p110δ Total
PI3K p110δ (Tyr485)
PTEN Total
RET Total
RET (Tyr826)
RET (Tyr62/90/96)
SLP65 Total
SLP65 (Tyr84)
SLP65 (Tyr189)
Syk Total
Syk (Tyr323)
Syk (Tyr629/630)
VEGFR1 Total
VEGFR1 (Tyr1169)
VEGFR2 Total
VEGFR2 (Tyr1175)
* Coming Soon
Magnetic Bead Format
▼ Panel Premix Only
MAPmates™ continued Sample Preparation Kit(Cat. No. MPEQ-SP)
A sample prep kit containing all the reagents you need to prepare up to 5 mL of sample lysates, including:• Cell lysis• Protein denaturing• Protein fragmentationRequired to run EpiQuant™ multiplex assays
See our website for Single Plex Kit catalogue numbers.18
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.M
ILLI
PLEX
® m
ap E
PIQ
UA
NT™ MILLIPLEX® map EpiQuant™
Phosphotyrosine Cell Signaling Panel 1 (Cat. No. MPEQ-100K)
Cbl (Tyr700/731/774)
Crk (Tyr221/239)
CUB domain containing protein 1 (Tyr734)
EGFR (Tyr1110/1125)
Ezrin (Tyr477)
FAK (Tyr397/407)
FAK (Tyr861)
FRS2 (Tyr436)
Fyb (Tyr651/697)
Gab1 (Tyr285/307/317)
Gab2 (Tyr266)
Gab2 (Tyr584)
Growth hormone receptor (Tyr566)
HER2 (Tyr1023)
HGFR (Tyr1003)
IL-15Ra chain (Tyr227))
IRS1 (Tyr612)
ITK (Tyr180)
JAK3 (Tyr785)
JNK 1/2/3 (Tyr185/185/223
LAT (Tyr255)
p38/MAPKa (Tyr181)
PDK1 (Tyr373/376)
PI3K p110δ (Tyr485)
Plakoglobin/a-catenin (Tyr548)
PLCγ1 (Tyr1253)
PLCγ2 (Tyr1197/1217)
Raf-1 (Tyr340/341)
Rap GEF1/GRF2 (Tyr504)
SCF38 (Tyr20)
SH2 domain containing protein 3A (Tyr231)
SH2 domain containing protein 3A (Tyr95)
Shc (Tyr349/350)
SPTAN1 (Tyr1176)
STAT3 (Tyr705)
Transferrin Receptor (Tyr20)
Tyrosine Protein Kinase Tec (Tyr206)
VEGFR1 (Tyr1169)
WASP (Tyr291)
ZAP70 (Tyr126)
Phosphotyrosine Cell Signaling Panel 2 (Cat. No. MPEQMAG-102K)
AXL (Tyr772)
AXL (Tyr859)
BTK (Tyr344)
EPOR (Tyr426)
GHR (Tyr487)
GSK3a/b (Tyr 216/279)
Heterogeneous nuclear ribonucleoprotein (Tyr72)
HGFR/met (Tyr406/407)
Kit/SCFR (Tyr545)
Kit/SCFR (Tyr936)
LSR (Tyr406/407)
LSR (Tyr615/616)
PSTPIP1 (Tyr344)
Rai-like protein (Tyr424)
Rai-like protein (Tyr465)
RET (Tyr1062/1090/1096)
RET (Tyr826)
Ron/MSP-R (Tyr1353-1360)
SYK (Tyr629/630)
Vanilloid receptor-like channel 2 (Tyr411)
Vav1 (Tyr142/160/174)
VEGF R3 (Tyr1230/1231)
* Coming Soon
Magnetic Bead Format
Human MAPK PathwayMB Format Coming Soon*
ATF2 Total
ATF2 (Ser328)
Bcl-2 (Thr56)
c-Fos Total
c-Fos (Thr232)
CHOP/GADD153 Total
CHOP/GADD153 (Ser79)
c-Jun Total
c-Jun (Tyr170)
c-Myc Total
c-Myc (Thr58)
ERK1 Total
ERK1 (Tyr204)
ERK2 Total
ERK2 (Tyr187)
ERK5 Total
ERK5 (Tyr221)
FAK Total
FAK (Tyr397/861)
HSF1 (Ser307)
HSP27 (Ser15)
IKKa (CHUK) (Ser176)
JNK1/2/3 Total
JNK1/2/3 (Tyr185)
MEK2 (Ser222/226)
MEK3/6 (Thr211/221)
MEK4 (Ser80)
MEK4 (Thr261)
MSK1 (Ser212)
NFkB1 Total
NFkΒ1 (Thr931)
p38a Total
p38a (Tyr185)
p38γ Total
p38γ (Tyr185)
Paxillin Total
Paxillin (Tyr31)
PRAK (Thr182)
RSK1 Total
RSK1 (Ser363)
Shc Total
Shc (Tyr349)
STAT3 Total
STAT3 (Tyr705)
Phosphothreonine Cell Signaling Panel 1 (Cat. No. MPEGMAG-103K)
AKT1 substrate 1 (Thr246)
CAM kinase 1a (ThrT177)
CAMKKa (Thr108)
CDC34 (Thr233)
ERa (Thr311)
Glutamine dependent carbamoylphosphate synthase (Thr1037)
IkBa (Thr291)
IKKa (Thr23)
IRS1 (Thr88)
NFkB1 (Thr931)
Occludin (Thr404)
Period circadian protein 3 (Thr635)
PKCe (Thyr566)
Myt1 (Thr495)
Ser/Thr protein kinase N (Thr780)
1919
Instrumentation & Software
millipore.com/analytes_Q1
INSTRUM
ENTATION
SOFTW
ARE
As a Luminex® partner, EMD Millipore is a
distributor of Luminex® instruments, accessories
and software. We were there at the beginning
to help validate Luminex® as a standard of
multiplexing, and our ongoing dedication
and experience in this technology enable you
to gain more information faster, without
compromising reliability.
Visit our website at www.millipore.com/luminex
for more information on the following
technologies:
NEW! MAGPIX® System(Cat. No. 40-042 - MAGPIX®) (Cat. No. 40-043 - MAGPIX® with MILLIPLEX® Analyst Software)
Combined with our MILLIPLEX® Map magnetic
bead-based multi-analyte panels, analysis
software and technical support, the new
Luminex® MAGPIX® system provides a complete
solution for rapid, accurate biomarker quantitation
in a variety of sample matrices. This affordable
system can perform up to 50 tests simultaneously
in a single reaction volume, greatly reducing
sample input, reagents and labor while improving
productivity. The MAGPIX® system features an
innovative design based on CCD imaging
technology that enables for a more compact,
robust system. In addition, it is easy to operate
and maintain with streamlined start-up and
shutdown protocols and minimal maintenance
requirements.
20
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.
20
Luminex® 200™ System(Cat No. 40-012- MILLIPLEX® Analyzer) (Cat No. 40-013 - MILLIPLEX® Analyzer with MILLIPLEX® Analyst Software)
MILLIPLEX® Analyzer, the Luminex® 200™ System,
is a compact lab analysis system that integrates
lasers, optics, fluidics, a controller, advanced
digital signal processing, an XY Platform, the
newest version analysis software, and a PC with
monitor. Specifically designed to enhance the
use of xMAP® technology, this system enables
simultaneous assays of up to 100 analytes per
well of a microtiter plate. Key features include:
flexible data analysis package, user friendly
functions, flexible assay design features, bar code
reader for enhanced efficiency and multiple
formats for generating data reports.
MILLIPLEX® Analyst Software(Cat. No. 40-266 - For a single computer) (Cat. No. 40-265 - For three computers)
Data Analysis Software, MILLIPLEX® Analyst,
developed exclusively for EMD Millipore by
VigeneTech™, provides a whole new level of data
analysis, incorporating tried-and-true algorithms
to provide the performance you need, even when
faced with the most challenging multiplexing
data sets. Also included are report formats and
enhanced presentation features that you’ve
requested to display your information clearly
and accurately.
Luminex® FLEXMAP 3D® System(Cat. No. 40-014 - FLEXMAP 3D®) (Cat. No. 40-022 - FLEXMAP 3D® with MILLIPLEX® Analyst Software)
The FLEXMAP 3D® system combines
differentially dyed fluorescent microsphere
sets with an innovative instrument design that
can accommodate measuring the concentrations
of up to 500 unique analytes within a single
sample rapidly and precisely. Features—such as
automated probe height adjustment, simplified
routine maintenance operations and an intuitive
software interface—make FLEXMAP 3D® a flexible
and easy-to-use multiplexing system.
INST
RUM
ENTA
TION
SO
FTW
ARE
21
For Research Use Only.
21
INSTRU
MEN
TATIO
N / SO
FTWA
RE
NEW! ELx50/8M Microplate Strip Washer for 96-Well Biomagnetic Separation(Cat. Nos. are based on power/power cord requirements. Please confirm with your sales representative which item will suit your needs.)
EMD Millipore, the leading provider of multiplex
immunodetection kits, in partnership with
BioTek®, the world leader in microplate washing,
now offers the new ELx50/8M microplate strip
washer for biomagnetic separation and washing
of MILLIPLEX® Map magnetic bead assays.
The ELx50/8M Microplate Washer is a welcome
upgrade from manual processing that brings to
your lab an all inclusive wash solution with
consistent performance and unattended
operation.
Handheld Magnetic Separator Block for 96-Well Flat Bottom Plates or 96-Well Conical Well Plates(Cat. No. 40-285)
The Handheld Magnetic Separator Block offers
a low cost option for biomagnetic separation.
Magnets are positioned around the bottom of the
well to pull the beads to the side of the well. The
top magnetic frame is white polycarbonate, with
a corrosion resistant steel plate underneath, all
mounted to a polypropylene base. An adjustable
clip system holds a wide variety of microplates to
the separator block, and an O-Ring on the base
plate facilitates gripping.
MultiScreen® HTS Filter Plates 96-well filter plates for sample isolation and purificationCat. No. MSBVN1B50 (50-pk. opaque plates) Cat. No. MX-PLATE (2-pk. opaque plate)
At every stage of the drug discovery process
samples must be isolated, purified and prepared
prior to testing. In today's fast paced environment,
rapid, automated and simple sample preparation
is a must. MultiScreen® plates offer diverse and
versatile formats for a variety of preparative
methods.
22
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.
Scepter™ 2.0Monitor immune cell types in seconds.
Integrated Display
• Histogram data on cell
populations
• Cell concentration, mean
cell volume, and cell size
• Can apply custom gating
• Gain insight into cell health
Plastic Consumable Sensors • Detect cells of varying size
ranges with 2 sensor types
• Discriminate cell sizes with
sub-micron resolution
• Discriminate cell volumes
with sub-picoliter resolution
Handheld Pipette
• Compact, easy to use
• Ergonomic action feels like
pipetting
• On-screen instructions
Scepter™ Features
Discover the fastest, easiest way to count and differentiate subsets of immune cells with the industry’s finest instrument for effortless cell analysis.
Scepter™, the first handheld automated cell counter, brought portable and precise cell counting to the palm of your hand. Scepter™ 2.0 expands your ability to precisely count particles as small as 3 µm, so you can analyze red blood cells, lymphocytes, monocytes and T-cell activation.
For more information, please visit:
www.millipore.com/scepter2.0
CELL
ULAR
AN
ALYS
IS
23
For Research Use Only.
easyCyte™ Benchtop Flow Cytometry
FlowCellect™ KitsFlowCellect™ Kits are fully optimized for "plug
and play" cellular analysis on guava® and other
flow cytometers.
Each kit has a unique combination of directly
conjugated antibodies, and/or fluorescent dyes
and protein reporters enabling you to:
• Monitor changes in cell health and autophagy
• Measure immune function
• Examine protein expression and post-
translational modifications
The kits also contain complete buffer sets,
protocols and pre-defined gate settings—
optimized to provide you with rapid and reliable
results through reduced sample preparation steps
and the elimination of compensation during
analysis.
For a complete listing of kits visit:
www.millipore.com/flowcytometry
Multiplexed cellular analysis from 5–8 parameters• Flow cytometry delivers robust, high-content
data in far less time than traditional methods
by measuring multiple parameters on
hundreds of cells per second.
• The compact guava® easyCyte™ line brings
this analytical power directly to the
laboratory bench with its novel microcapillary
technology enabling ease of use and lower
sample and reagent costs. These instruments
contain up to 2 lasers, and from 5–8
parameters and flexible formats for manual
or automated walk-away loading. This
complete end-to-end solution enables high
quality results and includes validated
reagents and full service support.
These technologies offer you the ability to:• Run minimal sample volumes without sheath
fluid using patented microcapillary
technology
• Synergistically compare data at the single cell
level with complementary extracellular
biomarker panels based on Luminex®
technology and the MAGPIX® system
• View up to 8 cellular parameters in real time
for multiplexed analysis and easily compare
different experiments
• Use optimized assays to expedite results and
avoid manual compensation altogether
CELLULAR ANALYSIS
24
Drug Discovery & Development
Speed your time to discovery by leveraging our
target profiling and clinical lab contract services
that help you prioritize potential drug candidates,
screen many biomarkers simultaneously, and
conduct research and clinical studies.
Lead Discovery SolutionsResults essential to your success
• Target Profiling
• Safety and Liability Testing
• Pathway Analysis
Contact the BioPharma Services group
serving your area and visit
www. millipore.com/BioPharma
for more information.
millipore.com/BioMarker_Services_Q1
BioPharma ServicesDedicated to large molecule bioanalysis
• Biomarker Analysis
• Immunogenicity Testing
• TK/PK Assays
• cGMP Services
NEW! UbiquitinProfiler™ ServiceAccelerate ubiquitin-related drug discovery with
our innovative, functional assay service that
reduces the complexity of the ubiquitination
pathway. EMD Millipore simplifies your route to
developing effective, safe ubiquitination-targeted
therapeutics. UbiquitinProfiler™ is the first and
only service that identifies potential activators
and inhibitors of E3 ligases within therapeutically
promising ubiquitination cascades.
Contact the Lead Discovery Services team at:
[email protected], or visit
www.millipore.com/LeadDiscovery.
North America
Phone: 1-800-645-5476
1-636-441-8400
Email: [email protected]
Europe
Phone: +44-(0)-1235-444166
Email: [email protected]
DRUG
DIS
COVE
RY
Discontinued Non-MagneticCatalogue No.
Kit DescriptionNon-Magnetic Bead Panel
Replacement Magnetic Bead Catalogue No.
Kit DescriptionMagnetic Bead Panel
CGT-98K Canine Gut Hormone CGTMAG-98K Canine Gut Hormone
HADCYT-61K Human Adipocyte HADCYMAG-61K Human Adipocyte
HADK1-61K-A Human Adipokine Panel A HADK1MAG-61K Human Adipokine Panel 1
HADK2-61K-B Human Adipokine Panel B HADK2MAG-61K Human Adipokine Panel 2
HBDP-33K Human Brain-Derived Panel HPTP1MAG-66K HPTP2MAG-66K
Human Pituitary Panels 1 and 2
HGAM-301 Human Immunoglobulin Isotyping HGAMMAG-301K Human Immunoglobulin Isotyping
HPT-66K Human Pituitary HPTP1MAG-66K HPTP2MAG-66K
Human Pituitary Panels 1 and 2
HSEP-63K Human Sepsis HSP1MAG-63K HSP2MAG-63K HSP3MAG-61K
Human Sepsis Panels 1, 2 and 3
RCYTO-80K Rat Cytokine RCYTOMAG-80K RCYTOMAG-80K-PMX
Rat Cytokine
RPT-86K Rat Pituitary RPTMAG-86K Rat Pituitary
25
For Research Use Only.
As we continue to expand our magnetic bead-based
multiplexing portfolio, we need to do some housekeeping
to make room for innovative and unique panels targeting
new species in emerging areas of research.
Consequently, we will be discontinuing some of our
non-magnetic bead kits by March 31, 2012*.
Below is a list of soon-to-be discontinued non-magnetic
bead panels and the corresponding replacement
magnetic bead panels.
Versatility in a Complete PackageMILLIPLEX® map Magnetic Bead-based Immunoassay KitsAs your research needs broaden, we’re growing with you.
For more information, please contact your local account manager at: www.millipore.com /offices
* Availability of inventory is subject to change depending on the depletion of existing inventory.
PANEL UPDATES
To Place an Order or Receive Technical AssistanceIn the U.S. and Canada, call toll-free 1-800-645-5476
For other countries across Europe and the world, please visit: www.millipore.com/offices
For Technical Service, please visit: www.millipore.com/techservice
Get Connected!Join EMD Millipore bioscience on your favorite socialmedia outlet for the latest updates, news, products,innovations and contests!
facebook.com/EMDMilliporeBioscience
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VigeneTech
www.emdmillipore.com
guava, Dual Detect CELISAs, MILLIPLEX, MultiScreen and OxyELISA are registered trademarks of Merck KGaA, Darmstadt, Germany.EMD Millipore, the M mark, ChemiKine, easyCyte, EpiQuant, FlowCellect, Scepter, MAPmates, and SignalProfiler are trademarks of Merck KGaA, Darmstadt, Germany.FLEXMAP 3D, Luminex, MAGPIX and xMAP are registered trademarks of Luminex Corporation.Luminex 200 is a trademark of Luminex Corporation.Biotek is a registered trademark of BioTek InsturmentsVigene Tech is a trademark of Vigene Tech, Inc.Lit. No. PF1089EN00 Rev. N 12/2011 LS-SBU-11-05730©2012 EMD Millipore Corporation, Billerica, MA, USA. All Rights Reserved.
