Download - AFFYMETRIX GENECHIP ARRAY STATION
AFFYMETRIX GENECHIP ARRAY STATION
Pierce Chuang, Vishu Rajamani, Jeremy RasiahBy
Overview
Introduction to DNA/RNA Sequencing Techniques Affymetrix GeneChip Station GeneChip Station Architecture Manufacturing Process Questions
Introduction to DNA/RNA
Introduction DNA/RNA (cont’d)
Genes
DNA Sequencing Techniques Chain-terminator method (Sanger)
Dideoxynuclotides triphosphates (ddNTPs) as DNA chain terminators
Sequence roughly 550,000 bases in a day Requires thirty years of uninterrupted
operation to sequence all nucleotides in the human genome
DNA Sequencing Techniques Sequencing By Hybridization (SBH)
Ex, target DNA sequence, AGCCTAGCTGAA
TTTCGGATCGAC
ATCGACTT
GATCGACT
GGATCGAC
CGGATCGA
TCGGATCGSequence Capacity Up to 300000 bases per
arrayProbe size 8 micron
Average Sequence Accuracy
> 99.9%
Sequencing required time
Less than 48 hours
Affymetrix Microarray Specification
Affymetrix GeneChip Station
Assay Preparation
Probes
GeneChip Array
source: http://bach2.biosci.arizona.edu/
Results
source: http://www.med.upenn.edu/
Affymetrix GeneChip Station Architecture
Detector Architecture
source: http://www.affymetrix.com/
GeneChip Array
source: http://bach2.biosci.arizona.edu/
GeneChip Array Cross Section
Array Manufacturing Process
2: substrates, SiO2, Si, GaAs, or Glass. 6: Linker Molecules. Ex, Aryl Acetylene 8: Mask, for photolithographic process 10: Activated Linker Molecules
Array Manufacturing Process
Array Manufacturing Process
This process is repeated several times to produce longer probes.
Full length probes are usually 25 nucleotides long
Questions ?