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EIpfmH[omMaCicsRsPftpspiPwConclusion: The results of this evaluation confirmed the clinical superiorityof [-2]proPSA and phi for the detection of PCa in the concentration rangeof 1.8 - 8.0 ng/mL total PSA. The phi index improves the detection of PCapatients with %fPSA > 25%.

doi:10.1016/j.jomh.2010.09.206

onclusion: The number of new clients of Klaipeda ACC and the numberf their visits to ACC was constantly growing throughout 1997-2006. Theumber of disinfection and contraception means, handed out to the ACClients, was increasing during 1997-2006 subject to the increasing numberf ACC clients. Consultations of social workers, preventive information,raining and consultation about the possibilities for treatment from therug addiction were most often held for IDU. The service of mediationnd directing of clients to other institutions and distribution of editionsbout safe sexual relations were carried out at least. The activity of ACCncouraged positive changes of IDU behaviour — it became safer bothn the field of drug use and in the field of sexual relations. The morerequently IDU visit ACC, the more real possibility of stopping drug usehey see.

oi:10.1016/j.jomh.2010.09.203

ISMH World Congress 2010 Abstract 203OES GENDER HEALTH DISPARITY BEGIN IN ADOLESCENCE?

. Ghannam, S. Shabsigh, D. Hajo, J. Jhaveri, R. Shabsigh ∗

Maimonides Medical Center, Brooklyn, USA

-mail address: RShabsigh@maimonidesmed.org (R. Shabsigh).ackground: Contributing to the health disparity between adult men andomen are health risk behaviors. Although potentially modifiable, a firsttep requires us to ask, when do these behavioral differences begin?ethods: Using data from the 2009 Youth Risk Behavior Surveillance Studye examined responses of 16,000+ American teenagers. Answers to a vari-ty of health risk behavior questions were analyzed for differences inrevalence between genders.esults: Compared to girls, boys were more likely to drive while drink-ng alcohol (11.6% n=7,890 vs. 7.6% n=8.173 p<0.001), carry a weapon27.1% n=7,848 vs. 7.1% n=8,203 p<0.001), and engage in fighting (39.1%= 7,890 vs. 22.9% n=8,183 p<0.001).ompared to girls, prevalence of recent tobacco use (29.8% n=7,301 vs.1.8% n=7,641 p<0.001) and “heavy” smoking (11.1% n=1,570 vs. 4.1%= 1,354 p<0.001) was more common in boys. Compared to girls, boysere more likely to experiment with drugs: marijuana (39.0% n=7,849s. 34.3% n=8,148 p<0.001), cocaine (7.3% n=7,927 vs. 5.3% n=8,214<0.001), heroin (3.2% n=7,750 vs. 1.7% n=7,923 p<0.001), ecstasy (7.6%= 7,811 vs. 5.5% n=8,016 p<0.001), methamphetamine (4.7% n=7,982s. 3.3% n=8,244 p<0.001), other intravenous drugs (2.7% n=7,923 vs..4% n=8,157 p<0.001).ompared to girls, boys weremore likely to havemultiple sexual partners16.2% n=7,261 vs. 11.2% n=7,672 p<0.001) and have sex while usingrugs or alcohol (25.9% n=2,692 vs. 17.1% n=2,829 p<0.001).6% of boys did not eat five servings of fruits and vegetables dailyn = 7,851). Over half of all boys did not have regular physical activity54.4% n=7,881). Prevalence of obesity was higher in boys than girls (15.3%= 7,572 vs. 8.3% n=7,606 p<0.001).onclusion: Regarding health behaviors among teenagers, boys demon-trated riskier behavior than girls. Injurious and violent behavior, tobaccond drug use, risky sexual practices and obesity were more prevalent inoys. Lifelong behavioral patterns are established in adolescence, possiblyecoming risk factors for chronic disease later in adulthood. Identifyingifferences in teen behavior allows for targeted intervention, bringing usloser to improving future outcomes of men’s health.

oi:10.1016/j.jomh.2010.09.204

ISMH World Congress 2010 Abstract 204ESTOSTERONE AUGMENTS POLYPHENOL-INDUCED DNA DAM-GE RESPONSE IN PROSTATE CANCER CELLS

isamitsu Ide, Lu Yan, Yu Jingsong, Satoru Muto, Shigeo Horie ∗

Teikyo University School of Medicine, Tokyo, Japan

-mail address: shorie@med.teikyo-u.ac.jp (S. Horie).ackground: Recently, we reported that combined ingestion of soysoflavones and curcumin significantly decreased serum level of prostate-pecific antigen based on a randomized placebo-controlled double-blindlinical study. We investigated whether these polyphenols inhibited

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ISMH Congress 2010 Abstracts

he proliferation of prostate cancer cells by activating a DNA damageesponse.aterials and Methods: The effects of isoflavones and curcumin on thexpression and phosphorylation of ATM (ataxia-telangiectasia-mutatedinase), H2AX (histone H2AX variant), and Chk2 (checkpoint kinase2)ere examined in LNCaP cells. The induction of apoptosis in LNCaP cellsas evaluated by poly(ADP-ribose) polymerase (PARP) cleavage. Further-ore, the effects of a testosterone supplement on modulation of the DNAamage response were examined.esults: Combined treatment of isoflavones and curcumin additivelyuppressed cellular proliferation and induced phosphorylation of ATM,istone H2AX, Chk2, and p53. Testosterone augmented the activation ofhe DNA damage response and PARP cleavage induced by curcumin.onclusion: Our results indicate that the activation of the DNA damageesponse by the polyphenols might suppress the malignant transforma-ion of prostate cancer. In addition, testosterone, when combinedwith theolyphenols, may have suppressive effects on the progression of prostateancer.

oi:10.1016/j.jomh.2010.09.205

ISMH World Congress 2010 Abstract 205HE [-2]PROPSA AND THE PROSTATE HEALTH INDEX (PHI)MPROVE THE DETECTIONOF PROSTATE CANCER FOR PATIENTSITH TOTAL PSA BETWEEN 1.8 AND 8.0 NG/ML

-S Blancheta,∗,1, A. Houlgatteb, X. Durandb, J.N. Ramirezc, K. Bensalahd,. Guilled, S. Vincendeaud

Beckman Coulter, Inc., Nyon, Switzerland, b Paris, Val de Grace Hospital, Depart-ent of Urology, c Paris, Val de Grace Hospital, Department of Biochemistry,Rennes, Hôpital Pontchaillou, Department of Urology

-mail address: jblanchet@beckmancoulter.com (J.-S. Blanchet).ntroduction: The benefit of screening for prostate cancer (PCa) using therostate specific antigen (PSA) as a biochemical marker is currently theocus of intense debate because of inadequate clinical performance. Beck-an Coulter has recently developed a new approach with the Prostateealth Index (phi)2 which combines the results of total PSA, free PSA and-2]proPSA, a molecular isoform of free PSA. The clinical performancef this index for the detection of PCa is currently being evaluated in aulticenter study.aterial and Methods: After six months of recruitment, 250 men (107 withnd 143 without PCa) with total PSA levels between 1.8 - 8.0 ng/mL (WHOalibration) and non-suspicious digital rectal examination (DRE) werencluded in the study. All PCa cases were confirmed by biopsies with 10ores or more. The total serum PSA, free PSA and [-2]proPSA were mea-ured on a Beckman Coulter DxI800 automated immunoassay analyzer.esults: Unlike the total PSA and free PSA, serum levels of [-2]proPSA wereignificantly higher in the PCa group compared with the group withoutCa. The parameters % free PSA, %[-2]proPSA and phiwere significantly dif-erent for the group of patients with PCa. A strong relationship betweenhe percentage of positive biopsies and phiwas confirmed. The diagnosticerformance of the 4 parameters by ROC curve analysis showed a highlyignificant superiority for %[-2]proPSA (AUC: 0.72) and phi (AUC: 0.72) com-ared to total PSA (AUC: 0.53) and % free PSA (AUC: 0.58). If prostate biopsyndication would have been based on %fPSA value with a 25% cut off, 14Ca would not have been detected. The phi index, with a cut of at 23,ould have detected 12 of these 14 cancers.

1 Not intended as off-label promotion of any BeckmanCoulter prod-ct.2 Not available in the United States.

jmh Vol. 7, No. 3, pp. 282–351, October 2010 345