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DNA Topoisomerases

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Page 1: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

DNA Topoisomerases

Page 2: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Review

Page 3: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

DNA Topoisomerases And

Cancer

Page 4: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Topoisomerases and TOP

Genes in Humans

Page 5: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Humans vs. Escherichia Coli

Page 6: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Humans vs. Escherichia Coli

Page 7: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Human Topoisomerases

Page 8: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Comparisons

Page 9: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Differential geometry

Page 10: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Top 1

Page 11: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Relaxation of DNA

Page 12: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Top1

Page 13: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

The Two Human Top 1s

Page 14: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Camptothecin

Page 15: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Camptothecins

Page 16: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Interfacial inhibitor

Page 17: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Mutation

Page 18: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Why New Top1 Inhibitors?

Page 19: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Pharmacological Limitations of

Camptothecins:

Page 20: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Indenoisoquinolines

Page 21: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Targeted delivery

Page 22: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Top2

Page 23: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Two Top2 enzymes

Page 24: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Top2

Page 25: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Transcription

Page 26: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

DNA replication

Page 27: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Top2 drugs

Page 28: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Etoposide

Page 29: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Levofloxacin

Page 30: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Interfacial inhibition

Page 31: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Topoisomerase drugs

Page 32: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Top 3

Page 33: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Decatenation

Page 34: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Top-3beta

Page 35: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Top3A and Top3B

Page 36: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Topoisomerases

Page 37: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Topoisomerases and disease

Page 38: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Replicative DNA damage

Page 39: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

DNA damage

Page 40: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

DNA damage

Page 41: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Human diseases

Page 42: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Covalent complexes

Page 43: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Topoisomerase

Page 44: DNA Topoisomerases - Center for Cancer Research · Levofloxacin. Interfacial inhibition. Topoisomerase drugs. Top 3. Decatenation. Top-3beta. Top3A and Top3B. Topoisomerases

Acknowledgements

Diamond DA 42 Twin Star G1000 Course Version 3beta · Compiled by Peter Schmidleitner Diamond DA 42 TwinGarmin G1000 Star Garmin 1000 Vers. 3

The Effect of Mutations in Type II Topoisomerases on

Catalytic mechanisms of topoisomerases DNA structure: Tertiary Structure: topology of plasmids Lecture 12: 1 10/25/2006

Direct measurement of DNA bending by type IIA topoisomerases

Clarke 1973 3beta-Phenyltropane-2-Carboxylic Esters and Analogs

“Dna topoisomerases in mt dna maintenance and ageing” and “dna damage checkpoints in nasopharyngeal carcinoma”

Rmi1 stimulates decatenation of double Holliday …microbiology.ucdavis.edu/kowalczykowski/PDF_files/Cejka...1 Rmi1 stimulates decatenation of double Holliday junctions during dissolution

dna replication - sciencetoat.weebly.com · DNA Replication Enzymes topoisomerases RNA primase DNA helicase DNA polymerase I ... • single-stranded binding proteins (SSBs) bind to

DNA Topoisomerases - National Cancer Institute · 2018. 11. 19. · Topoisomerases and disease . Replicative DNA damage . Human diseases . DNA repair Ataxia A medical condition that

A topological analysis of difference topology experiments of … · topology. Type II topoisomerases are not site specific; that is, they canbindanywherealongDNA.Usingexamples,weillustratesome

DNA Topoisomerases. DNA Supercoiling in vivo In most organisms, DNA is negatively supercoiled ( ~ -0.06) Supercoiling is involved in initiation of

DNA Topoisomerases maintain promoters in a state competent for transcriptional activation in Saccharomyces cerevisiae. 21 June 2013 Ph.D. student Jacob

pureportal.strath.ac.uk · Web viewIn Vitro Biological Evaluation of Novel Broad-Spectrum Isothiazolone Inhibitors of Bacterial Type II Topoisomerases Cédric Charrier,* Anne-Marie

Microheterogeneity of Topoisomerase IA/IB and Their DNA ...faculty.fiu.edu/~fernandf/pubs/117_2019_ACS_Omega... · Topoisomerases can assume a variety of conformations to regulate

oar.a-star.edu.sg · Web viewBacterial DNA topoisomerases are essential for bacterial growth and are attractive important targets for developing antibacterial drugs. Consequently,

In Vivo Catenation and Decatenation of DNAt - Molecular and

DNA TOPOISOMERASES Structure, Function, and … · TYPE IB DNA TOPOISOMERASES ... Their function remains obscure, ... Fork movement during replication of a circular DNA can generate

Inhibitors of topoisomerases as anticancer drugs: Problems ...nopr.niscair.res.in/bitstream/123456789/23529/1... · Review Article Inhibitors of topoisomerases as anticancer drugs:

Mutations in genes encoding condensin complex …genesdev.cshlp.org/content/30/19/2158.full.pdfMutations in genes encoding condensin complex proteins cause microcephaly through decatenation

dspace.umh.esdspace.umh.es/bitstream/11000/2242/1/TD López Hernández, Nicolás.pdf · AMS = Atrofia multisistema Beta-CIT 2beta-carbometoxi-3beta-(4-iodofenil)tropano BSIT = Brief

Genome biology of type II topoisomerases Michael Wilson SickKids Research Institute and Department of Molecular Genetics University of Toronto Epigenetics

Inibidores das DNA Topoisomerases como novos …...Sílvia Patrícia Ribeiro Dias Inibidores das DNA Topoisomerases como novos alvos terapêuticos para o Trypanosoma brucei (Doença

Catalytic mechanisms of topoisomerases

DNA topoisomerases em Leishmania sp.: potenciais alvos ... C Garc… · DNA topoisomerases em Leishmania sp.: potenciais alvos terapêuticos | 2014 2 Ana Cláudia Francisco Garcia

DNA Topoisomerases and Their Poisoning by Anticancer and Antibacterial Drugs Yves Pommier, Elisabetta Leo, HongLiang Zhang, Christophe Marchand Chemistry

DNA Topoisomerases · Topoisomerases and disease . Replicative DNA damage . Human diseases . DNA repair Ataxia A medical condition that is characterized by a lack of coordination

DNA Topoisomerases Maintain Promoters in a State …

International Journal for Parasitology: Drugs and Drug ... · Topoisomerases DNA topology Target-based drug discovery Chemotherapy abstract The Trypanosomatidae family, composed of

Single Supercoiled DNAs. DNA Supercoiling in vivo In most organisms, DNA is negatively supercoiled ( ~ -0.06) Actively regulated by topoisomerases,

DNAtopoisomerases - Oxford University Pressglobal.oup.com/booksites/content/0198506554/chapter5.pdfvaccinia. Examples of DNA topoisomerases are given in Table 5.1. Perhaps the most

7-Keto-Cholesterol and Cholestan-3beta, 5alpha, 6beta ... · Oxysterols, long time known as products of cholesterol metabolism, attracted much interest when it was suggested their

Discovery of Novel Glycogen Synthase Kinase-3beta

Levofloxacin | apollo +9191 46 950 950 Levofloxacinapollopharma.in/pdf/Levofloxacin.pdfpyelonephritis. Mechanism of action: Levofloxacin inhibits bacterial type II topoisomerases,

Top3-Rmi1 Dissolve Rad51-Mediated D Loops by a …microbiology.ucdavis.edu/kowalczykowski/PDF_files... · 2015. 3. 5. · the decatenation activity of Top3 while slowing DNA relaxation

Interplay between Type 1A Topoisomerases and Gyrase in ... · topoisomerases were absent (30). Since only topA mutants, not topBones,displayseveregrowthdefects,itispossiblethatthetopA