DNA is genetic material.DNA is genetic material.

TAATACGACTCACTATAGGGAGATAATACGACTCACTATAGGGAGA

TAATACGACTCACTATAGGGAGATAATACGACTCACTATAGGGAGA

Parts are basic biological functions Parts are basic biological functions that can be encoded as genetic material.that can be encoded as genetic material.

R0083R0083R0083R0083

R0083R0083

Type: Type: PromoterPromoterFamily:Family: Protein:DNA Protein:DNA Activity:Activity: 2 PoPS2 PoPSRequires: Requires: C0083C0083Cell Type:Cell Type: AnyAnyTemp:Temp: < Tm< TmIssues:Issues: NoneNoneLicense:License: PublicPublic

OtetOtetOtetOtet RBRBSS

RBRBSS Lambda cILambda cILambda cILambda cI TermTerm

..TermTerm

..

TetRTetR CICI

R0040R0040R0040R0040 B003B00344

B003B00344 C0051C0051C0051C0051 B001B001

55B001B001

55

C00C004040

C00C005151

R0040R0040R0040R0040 B003B00344

B003B00344 C0051C0051C0051C0051 B001B001

55B001B001

55

C00C004040

C00C005151

Devices are combinations of one or more Devices are combinations of one or more parts that encode human-defined parts that encode human-defined functions.functions.

C00C004040

C00C005151

C0040 to C0051 C0040 to C0051 INVERTERINVERTER

C0051 to C0051 to C0010 C0010

INVERTERINVERTER

C0010 to C0010 to C0040C0040

INVERTERINVERTER

Systems are combinations of one or more Systems are combinations of one or more devices that encode human-defined devices that encode human-defined functions.functions.

C0040 to C0040 to C0051 C0051

INVERTERINVERTER

R0040R0040R0040R0040 B003B00344

B003B00344 C0051C0051C0051C0051 B001B001

55B001B001

55

C00C004040

C00C005151

R0051R0051R0051R0051B003B00344

B003B00344 C0051C0051C0051C0051 B001B001

55B001B001

55

C00C005151

Endy DFrom Details to a Devicehttp://openwetware.org/wiki/BE.180:Devices

PoPS, Not Proteins!PoPS, Not Proteins!

Endy D, Deese I, Wadey CAdventures in Synthetic BiologyNature 24 November 2005http://openwetware.org/wiki/Adventures

SystemsSystems

DevicesDevices

PartsParts

DNADNA

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Device-level Device-level DiagramDiagram

Device-Level Device-Level TimingTiming

Parts-level Parts-level DiagramDiagram

Parts-level LayoutParts-level Layout