伯森生物科技股份㈲限公司Blossom Biotechnologies Inc.

伯森網站：www.blossombio.com / 客服專線：台北 0800-059668 台南 0800-259988 花東 0800-056668 / 電子信箱：blossom@blossombio.com.tw

台北 (02) 2788-2121 新竹 (037) 581-106 花東 0800-056668

台中 (04) 2323-3939 台南 (06) 235-6628 高雄 (07) 555-0764

HDAC & Sirtuin Enzyme Activity Assay Kits

Enzo�Life�Sciences�是全球第⼀個研發出⾮放射性� HDAC� (Histone�Deacetylase)� 酵素活性檢測試劑的公司，其所推出的各類組蛋⽩後轉譯修飾偵測試劑，皆採⽤⾼品質的專利受質及顯⾊劑� (sub-strate/developer)，同時搭配⾼活性、純度的酵素，讓研究者可以⾼效準確地篩選出潛⼒藥物。

產品優勢

l 偵測⽅式多元：包括可⾒光、化學冷光、及螢光三種⽅式可供選擇。

l 實驗流程簡單：全程不需清洗，適合⾼通量需求者。

l 避免⼲擾現象：專利研發的�®Chemilum� de� Lys � 化學冷

光受質，可有效降低各類�Inhibitors ,� Lys is� deter-gents� 所造成的⼲擾現象，避免偽陽性與偽陰性結果。

HDAC/Sirtuin Assay Kits

Eliminate Interference Seen in Luciferase-based Assays

Eliminate False HDAC/SIRT Screening Hits with Chemilum de Lys®

Competitor HDAC Assays

Luciferase-basedChemiluminescent Assay

Chemilumde Lys®

ColorimetricAssay

FluorometricAssay

No Artifactual activationby resveratrol

Resistant to Protease Inhibitors

Resistant toKinase Inhibitors

Colorimetric

Chemiluminescent

Fluorometric - General HDAC/SIRTUIN Assays

Fluorometric - Enzyme Specic HDAC/SIRTUIN Assays

Color de Lys® HDAC colorimetric activity assay kit

Chemilum de Lys® HDAC/SIRT Chemiluminescent drug discovery kit

Fluor de Lys® HDAC Cellular Activity Assay Kit

Fluor de Lys® HDAC Activity Assay Kit

Fluor de Lys® Green HDAC Activity Assay Kit

Fluor de Lys® HDAC1 Drug Discovery Kit

Fluor de Lys® Green HDAC2 Drug Discovery Kit

Fluor de Lys® HDAC3/NCOR1 Drug Discovery Kit

Fluor de Lys® HDAC6 Drug Discovery Kit

Fuor de Lys® HDAC8 Drug Discovery Kit

Fluor de Lys® SIRT1 Drug Discovery Kit

Fluor de Lys® SIRT2 Drug Discovery Kit

Fluor de Lys® SIRT3 Drug Discovery Kit

Fluor de Lys® SIRT5 Drug Discovery Kit

Fluor de Lys® Green SIRT5 Drug Discovery Kit

Product Type Product Name

BML-AK501

BML-AK532

BML-AK503

BML-AK500

BML-AK530

BML-AK511

BML-AK512

BML-AK531

BML-AK516

BML-AK518

BML-AK555

BML-AK556

BML-AK557

BML-AK513

BML-AK514

Cat. No.

⽬前已知異常的組蛋⽩修飾�(Histone�Modification)�與多種癌症及疾病具有⾼度相關性,�因此可藉由找尋調控此機制的⽅式,�開發具有潛⼒的治療藥物。本刊主要針對組蛋⽩修飾相關分析⼯具進⾏介紹,�所有產品皆來⾃國際知名⼤廠��Enzo�Life�Sciences,�Eurofins�Scientific,�及�PerkinElmer。歡迎踴躍洽詢伯森業務專員索取更詳盡的表觀遺傳學研究產品資訊,�以及最新優惠訊息。

EPIGENETICS RESEARCH TOOLSEPIGENETICS RESEARCH TOOLSEPIGENETICS RESEARCH TOOLSEPIGENETICS RESEARCH TOOLS

The compound, PTC124, is converted

by luciferase in the presence of ATP to

a high afnity multi-substrate adduct

inhibitor, PTC124-AMP. PTC124 at

concentrations greater than 1 µM

inhibits the luciferase based assay, but

has no effect on the Chemilum de

Lys® assay.

PTC124

0 µM 0.01 µM 0.1 µM 1 µM 10 µM 100 µM

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

1.1

0.0

RL

U

Competitor P®

Chemilum de Lys

伯森網站：www.blossombio.com / 客服專線：台北 0800-059668 台南 0800-259988 花東 0800-056668 / 電子信箱：blossom@blossombio.com.tw

® ®AlphaLISA and LANCE Ultra Assays

Biochemical Epigenetic Assays

® ®由美商�PerkinElmer�獨家研發的�AlphaLISA �及�LANCE �Ultra�系列

產品，可依據您的需求彈性設計實驗，分析過程無需清洗或分離步

驟，能夠⼤幅簡化並縮短實驗時間；同時具有提⾼偵測靈敏度、節

省珍貴樣品⽤量的優點，特別適合有⾼通量藥物篩選需求的研究者

使⽤。

產品特點

®l AlphaLISA ：⾼靈敏度、應⽤彈性，可應⽤於�Biochemical�與�Cell-based�assay。�(Peptide�與�Full-length�protein�型式的酵素受質皆可適⽤偵測。)

®l LANCE �Ultra：極⾼靈敏度，適⽤於�Biochemical�assay。�(僅適⽤於�Peptide�型式的酵素受質，不適⽤於全⾧蛋⽩。)

® ®利⽤�AlphaLISA �及�LANCE �Ultra�技術，搭配專⼀性的�Peptide�作為酵素受質�(enzyme�substrate)，以偵測甲基化�

(methylation)、或⼄醯化�(acetylation)�等修飾酵素活性。可作為化合物或藥物篩選平台。

High-throughput Epigenetic Assays

Detection of reaction products occurs via mark-specic antibodies coupled to Acceptor beads (AlphaLISA®) or labeled with europium chelate

(LANCE® Ultra). The biotin substrates is captured by streptavidin coupled to a Donor bead (AlphaLISA®) or labeled with the ULight™ acceptor dye

(LANCE® Ultra). In both assay technologies, irradiation of the captured reaction products triggers an energy transfer leading to light emission

proportional to the enzyme activity.

AlphaLISA® LANCE® Ultra

伯森網站：www.blossombio.com / 客服專線：台北 0800-059668 台南 0800-259988 花東 0800-056668 / 電子信箱：blossom@blossombio.com.tw

Cell-based Epigenetic Assays

®針對細胞萃取物�(cell�lysates)�樣本的偵測實驗，具有多款便利的�AlphaLISA �偵測試劑套組可供選擇，可直接適⽤於

偵測�endogenous�histone�protein�修飾情形。

Schematic representation of the AlphaLISA®

cellular assay for the detection of modied histone

proteins. Histone H3 is extracted from cell lysates

and detected with a biotinylated Anti-H3 antibody

which binds streptavidin Donor beads and anti-mark

antibody-coated AlphaLISA® Acceptor beads

specic to the particular modication (acetylated or

methylated residue).

Order Information

H3K4 unmodied

H3K9/K27 unmodied

H3K4me1-2

H3K9ac

H3K9me2

H3K27ac

H3K27me2-1

H3K27me3

H3K36me2

H3K79me2

H3R2me

H3/H4Rme pan

H4Kac pan

H4R3me

H4K20me1

O-linked GlcNAc

P53 K382ac

LSD1, Sirtuin 1, HDAC2

HDAC1, JMJD1A

SET7/9

p300

G9a, JMJD2A, JMJD2C

HDAC1

EZH2, JMJD3

EZH2

JMJD2A

DOT1L

PRMT6

PRMT4

KAT5

PRMT1

SET8

OGA

Sirtuin 1

AL119

AL138

AL116

AL114

AL117

AL120

AL121

AL122

AL123

AL148

AL139

AL151

AL143

AL150

AL145

AL144

AL124

AL719

AL716

AL714

AL717

AL720

AL721

AL722

AL723

AL748

TRF0404

TRF0411

TRF0402

TRF0400

TRF0403

TRF0405

TRF0406

TRF0407

TRF0408

TRF0415

TRF0412

TRF0414

TRF0413

TRF0409

Mark Detected Validated EnzymeAlphaLISA

1(Biochemical)AlphaLISA

(Cell-based)LANCE Ultra

2(Biochemical)

(1). Additional Items Required for AlphaLISA histone modication biochemical assays: AlphaScreen Streptavidin Donor beads (Cat# 6760002),

AlphaLISA biotin-anti-histone H3 (C-ter) antibody (required for full-length Histone H3 substrates) (Cat# AL118), AlphaLISA biotin-anti-histone

H4 antibody (required for full-length Histone H4 substrates) (Cat# AL146), and AlphaLISA 5X Epigenetics Buffer 1 kit (Cat# AL008).

(2). Additional Items Required for LANCE Ultra histone modication assays: LANCE Ultra ULight-Streptavidin (Cat# TRF0102), and LANCE

Detection Buffer, 10X (Cat# CR97-100).

更多 Epigenetics 研究工具歡迎洽詢伯森業務專員 O 0800-059668

伯森網站：www.blossombio.com / 客服專線：台北 0800-059668 台南 0800-259988 花東 0800-056668 / 電子信箱：blossom@blossombio.com.tw

Epigenetic Proteins & Enzymes

全球知名⽣藥檢測服務⿓頭�Eurofins�Scientific�公司，提供⼀系列品質穩定的

表觀遺傳學重組蛋⽩及酵素。每批產品於出廠前皆經過嚴格控管以確保⾼純度® ®與⾼活性。可搭配�PerkinElmer�AlphaLISA �及�LANCE �Ultra�兩⼤技術平台，

進⾏藥物篩選實驗。

Epigenetic Proteins & Enzymes

l JMJD2A

l JMJD2B

l JMJD2C

l JMJD2D

l JMJD2E

l JMJD3

l hPHF8

l Sirtuin 1

l Sirtuin 2

l DNMT1

l DOT1L

l EHMT1

l EZH2

l G9a

l HAT1

l NSD1

l p300

l PRMT1

l PRMT3

l PRMT4

l SET7

l SET9

l SETD2

l SETD7

l SETD8

l SUV4-20H2

l NSD3 / WHSC1L1 (h)

l ASH1L

l ATAD2A

l ATAD2B

l BAZ2B

l BRD2(1)

l BRD2(2)

l BRD3(1)

l BRD3(2)

l BRD4(1)

l BRD4(2)

l BRDT(1)

l CECR2

l CREBBP

l FALZ

l KAT2A (GCN5L2)

l PB1(2)

l PB1(4)

l PB1A(3)

l PCAF

l PHIP(2)

l SMARCA2

l SMARCA4

l SP140

l TAF1(1)

l TAF1(2)

Readers (Bromodomain) Erasers

《 Epigenetics Proteins & Enzymes 產品項目列表 》

LIT. NO.: DM201521IMAGE: Enzo Life Sciences,

Eurofins Scintific, PerkinElmer

伯森生物科技股份㈲限公司Blossom Biotechnologies Inc.

台北 (02) 2788-2121 新竹 (037) 581-106 花東 0800-056668

台中 (04) 2323-3939 台南 (06) 235-6628 高雄 (07) 555-0764

Writers

Other Highly Active HDAC and Sirtuin Proteins

HDAC (rat liver)

HDAC1 (human), (recombinant) (His-tag)

HDAC2 (full-length) (human), (recombinant) (His-tag)

HDAC2 (human) (1-488), (recombinant) (His-tag)

HDAC3 (human), (recombinant) (His-tag)

HDAC3 / NCOR1 complex (human), (recombinant)

HDAC6 (human), (recombinant) (His-tag)

ALX-202-052

BML-SE456

BML-SE533

BML-SE500

BML-SE507

BML-SE515

BML-SE508

Product Name Cat. No.

HDAC8 (human), (recombinant)

HDAC10 (human) (recombinant) (Histag)

HDAC11 (human) (recombinant) (Histag)

SIRT1 (human), (recombinant) (His-tag)

SIRT2 (human), (recombinant) (His-tag)

SIRT3 (human), (recombinant) (His-tag)

SIRT5 (human), (recombinant) (His-tag)

Product Name

BML-SE145

BML-SE559

BML-SE560

BML-SE239

BML-SE251

BML-SE270

BML-SE555

Cat. No.