Disk Stack Centrifuge

Presented By:

Gureet Chandhok

Waqar Khan

Blake Mc Cord • CHEE 450: Engineering Biology

DISK STACK CENTRIFUGE

•A centrifuge is a piece of equipment, generally driven by a motor, that puts an object in rotation around a fixed axis, applying force perpendicular to the axis.

•Essentially works like a clarifier providing better control over residence time.

Purpose of Centrifugation

• Since ours is a fermentation based bio process, the ISOLATION and the purification of the Insulin is the immediate goal once the fermentation step is concluded.

• In Insulin production, used to separate desired proteins from unwanted biomass.

Types Of Centrifuges

Tricanter

Disk Stack Centrifuge

Decanter

Sedicanter

Belt Press

Sorticanter

How does it Work?

• Disks are spaced out at 2mm.• Spins rapidly exerting upto

12000 G force.• Solid collects at the bottom

liquids move up.

Operating Factors

• Solid holding space

• Viscosity

• Diameter of particle

• Difference in Density

d2(ρp- ρ1)gvt = ------------- 18μStokes Law!

Design Improvements

Factors governing performance

• Volume of solids-Coagulation and Flocculation

• Settling Area-Residence Time

CH-36B GOF ®

• Manufacturer-Alfa Laval• Type – Nozzle Centrifuge with continuous

discharge• Able to handle sensitive cell cultures• Recirculation of solids• Gentle gradual acceleration of product• Minimization of shear forces preventing cell

lysis• No contact with oxygen• No foaming or oxidation of product• High grade stainless steel• Steam sterilizable

Design Specifications

• Feed Rate-3888.81 kg/h• Viscosity of Liquid-1.5 cP• Height-3.85m• G-4300• Speed- 2900 rpm• Sedimentation Efficiency-30%• Motor Power-26 kW• Volume- 18.5m3

Co$t

• Estimated to be ~$350,000-$650,000

• Operating costs extra

References

• http://www.flottweg.de/Broschu/%DCbersichtsprospekt_E.pdf• http://www.lsbu.ac.uk/biology/enztech/centrifugation.html#2_2• http://www.lenntech.com/Centrifugation.htm • www.fao.org/docrep/003/x6899e/X6899E12.jpg • http://www.alfalaval.com/digitalassets/2/file10080_0_PD41493e.pdf• http://www.alfalaval.com/ecoreJava/WebObjects/ecoreJava.woa/wa/

showNode?siteNodeID=8720&contentID=25790&languageID=1

Questions?