disk stack centrifuge presented by: gureet chandhok waqar khan blake mc cord chee 450: engineering...
TRANSCRIPT
![Page 1: Disk Stack Centrifuge Presented By: Gureet Chandhok Waqar Khan Blake Mc Cord CHEE 450: Engineering Biology](https://reader035.vdocuments.mx/reader035/viewer/2022062519/56649cbb5503460f94982f0a/html5/thumbnails/1.jpg)
Disk Stack Centrifuge
Presented By:
Gureet Chandhok
Waqar Khan
Blake Mc Cord • CHEE 450: Engineering Biology
![Page 2: Disk Stack Centrifuge Presented By: Gureet Chandhok Waqar Khan Blake Mc Cord CHEE 450: Engineering Biology](https://reader035.vdocuments.mx/reader035/viewer/2022062519/56649cbb5503460f94982f0a/html5/thumbnails/2.jpg)
DISK STACK CENTRIFUGE
•A centrifuge is a piece of equipment, generally driven by a motor, that puts an object in rotation around a fixed axis, applying force perpendicular to the axis.
•Essentially works like a clarifier providing better control over residence time.
![Page 3: Disk Stack Centrifuge Presented By: Gureet Chandhok Waqar Khan Blake Mc Cord CHEE 450: Engineering Biology](https://reader035.vdocuments.mx/reader035/viewer/2022062519/56649cbb5503460f94982f0a/html5/thumbnails/3.jpg)
![Page 4: Disk Stack Centrifuge Presented By: Gureet Chandhok Waqar Khan Blake Mc Cord CHEE 450: Engineering Biology](https://reader035.vdocuments.mx/reader035/viewer/2022062519/56649cbb5503460f94982f0a/html5/thumbnails/4.jpg)
Purpose of Centrifugation
• Since ours is a fermentation based bio process, the ISOLATION and the purification of the Insulin is the immediate goal once the fermentation step is concluded.
• In Insulin production, used to separate desired proteins from unwanted biomass.
![Page 5: Disk Stack Centrifuge Presented By: Gureet Chandhok Waqar Khan Blake Mc Cord CHEE 450: Engineering Biology](https://reader035.vdocuments.mx/reader035/viewer/2022062519/56649cbb5503460f94982f0a/html5/thumbnails/5.jpg)
Types Of Centrifuges
Tricanter
Disk Stack Centrifuge
Decanter
Sedicanter
Belt Press
Sorticanter
![Page 6: Disk Stack Centrifuge Presented By: Gureet Chandhok Waqar Khan Blake Mc Cord CHEE 450: Engineering Biology](https://reader035.vdocuments.mx/reader035/viewer/2022062519/56649cbb5503460f94982f0a/html5/thumbnails/6.jpg)
How does it Work?
• Disks are spaced out at 2mm.• Spins rapidly exerting upto
12000 G force.• Solid collects at the bottom
liquids move up.
![Page 7: Disk Stack Centrifuge Presented By: Gureet Chandhok Waqar Khan Blake Mc Cord CHEE 450: Engineering Biology](https://reader035.vdocuments.mx/reader035/viewer/2022062519/56649cbb5503460f94982f0a/html5/thumbnails/7.jpg)
![Page 8: Disk Stack Centrifuge Presented By: Gureet Chandhok Waqar Khan Blake Mc Cord CHEE 450: Engineering Biology](https://reader035.vdocuments.mx/reader035/viewer/2022062519/56649cbb5503460f94982f0a/html5/thumbnails/8.jpg)
Operating Factors
• Solid holding space
• Viscosity
• Diameter of particle
• Difference in Density
d2(ρp- ρ1)gvt = ------------- 18μStokes Law!
![Page 9: Disk Stack Centrifuge Presented By: Gureet Chandhok Waqar Khan Blake Mc Cord CHEE 450: Engineering Biology](https://reader035.vdocuments.mx/reader035/viewer/2022062519/56649cbb5503460f94982f0a/html5/thumbnails/9.jpg)
Design Improvements
Factors governing performance
• Volume of solids-Coagulation and Flocculation
• Settling Area-Residence Time
![Page 10: Disk Stack Centrifuge Presented By: Gureet Chandhok Waqar Khan Blake Mc Cord CHEE 450: Engineering Biology](https://reader035.vdocuments.mx/reader035/viewer/2022062519/56649cbb5503460f94982f0a/html5/thumbnails/10.jpg)
CH-36B GOF ®
• Manufacturer-Alfa Laval• Type – Nozzle Centrifuge with continuous
discharge• Able to handle sensitive cell cultures• Recirculation of solids• Gentle gradual acceleration of product• Minimization of shear forces preventing cell
lysis• No contact with oxygen• No foaming or oxidation of product• High grade stainless steel• Steam sterilizable
![Page 11: Disk Stack Centrifuge Presented By: Gureet Chandhok Waqar Khan Blake Mc Cord CHEE 450: Engineering Biology](https://reader035.vdocuments.mx/reader035/viewer/2022062519/56649cbb5503460f94982f0a/html5/thumbnails/11.jpg)
Design Specifications
• Feed Rate-3888.81 kg/h• Viscosity of Liquid-1.5 cP• Height-3.85m• G-4300• Speed- 2900 rpm• Sedimentation Efficiency-30%• Motor Power-26 kW• Volume- 18.5m3
![Page 12: Disk Stack Centrifuge Presented By: Gureet Chandhok Waqar Khan Blake Mc Cord CHEE 450: Engineering Biology](https://reader035.vdocuments.mx/reader035/viewer/2022062519/56649cbb5503460f94982f0a/html5/thumbnails/12.jpg)
Co$t
• Estimated to be ~$350,000-$650,000
• Operating costs extra
![Page 13: Disk Stack Centrifuge Presented By: Gureet Chandhok Waqar Khan Blake Mc Cord CHEE 450: Engineering Biology](https://reader035.vdocuments.mx/reader035/viewer/2022062519/56649cbb5503460f94982f0a/html5/thumbnails/13.jpg)
References
• http://www.flottweg.de/Broschu/%DCbersichtsprospekt_E.pdf• http://www.lsbu.ac.uk/biology/enztech/centrifugation.html#2_2• http://www.lenntech.com/Centrifugation.htm • www.fao.org/docrep/003/x6899e/X6899E12.jpg • http://www.alfalaval.com/digitalassets/2/file10080_0_PD41493e.pdf• http://www.alfalaval.com/ecoreJava/WebObjects/ecoreJava.woa/wa/
showNode?siteNodeID=8720&contentID=25790&languageID=1
![Page 14: Disk Stack Centrifuge Presented By: Gureet Chandhok Waqar Khan Blake Mc Cord CHEE 450: Engineering Biology](https://reader035.vdocuments.mx/reader035/viewer/2022062519/56649cbb5503460f94982f0a/html5/thumbnails/14.jpg)
Questions?