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DiPel ® Biological Insecticide Horticulture Crops Technical Manual Innovative solutions. Business made easy.

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Page 1: DiPel - fingal.ca · 1. The Bt toxin crystals dissolve in the midgut. 2. The Bt toxins are activated and attach to midgut receptors. 3. Pores form in the cell wall and cells rupture

DiPel®

Biological Insecticide

Horticulture CropsTechnical Manual

Innovative solutions. Business made easy.

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Page 2: DiPel - fingal.ca · 1. The Bt toxin crystals dissolve in the midgut. 2. The Bt toxins are activated and attach to midgut receptors. 3. Pores form in the cell wall and cells rupture

Aim:Getting safe, reliable, cost-effective control of Lepidoptera pests in a full spectrum of horticulture crops (including conventional and organic production).

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Introduction:DiPel is the number one biological insecticide in the world. This potent Bacillus thuringiensis (Bt) insecticide provides proven control of Lepidoptera pests in many crops. Its balanced blend of four active toxins, plus a natural spore, provides exceptional control. This varied list of active toxins also makes DiPel an effective resistance management tool.

DiPel can be applied in conventional or organic systems. Because it is Exempt from Tolerance, DiPel gives grower’s full confidence when selling crops to foreign markets. In addition, DiPel’s superior, high-potency, dry-flowable formulation ensures the longest shelf life of any other Bt on the market.

Benefits of Using Diepel:• Itdetersthewormfromfeedingimmediately,protectingthecropfrom

damage. Death occurs within 1 to 3 days.

• DiPel’sdry-flowableformulationisconvenientandeasytohandle.

• DiPel’suniquemodeofactionisanexcellenttoolformanagingresistance of many pests.

• Itcanbereadilytankmixedtoimprovetheperformanceoftraditional insecticides or used as a rotation partner in conjunction with a traditional insecticide program.

• UsingDiPelcanincreasetheoverallvalueofyourinsectcontrolprogram.Growerscanenjoyimprovedperformanceatequalorlesscosts.

• DiPelisnotharmfultobeesorbeneficialinsectsmakingitanexcellent tool for insect management at bloom time as well as in an intergraded pest management programs (IPM).

• Nore-entryintervalrestriction,allowingquickaccesstoyourcrops.

• ExemptfrommaximumresiduelimitsinCanada so you can apply up to harvest.

• Residuallastbetween7and10days depending on formulation.

• Rainfastnessis3hours.

• Safetohumans and crops.

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Other Benefits of using DiPel: The safety and environmental profile of DiPel insectide results in no re-entry intervals and the shortest pre-harvest intervals (PHI) allowed by law. This gives growers great flexibility throughout the growing season.

In addition, DiPel is exempt from tolerance, which means growers don’t need to worry about selling into export markets due to residue issues.

DiPel is OMRI-certified for use in organic production. It provides organic growers with a proven and highly effective natural tool to fight insect pests.

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Mode of Action: DiPel is created with a naturally occurring bacterium found in soil and on plant leaves throughout most regions of the world. It contains insecticidal proteins and spores that become active inside the insect’s gut when ingested by Lepidopteran larvae.

The most potent Bt products deliver multiple modes of action in the insect’s gut, which make it a valuable tool in resistance management. In fact, many new insecticide technologies are being met with resistance – emphasizing the need for Bt insecticides.

BeingthestrongestBtproductonthemarketDiPelgoestoworkquickly.Onceingested, the Bt toxins bind to the midgut cells, creating performances in the gut lining and causing the insect to stop feeding within several minutes. Bt spores then penetrate the body cavity and circulatory systems. The spores germinate, spread through the body and cause poisoning. Insects start to die one to three days after ingestion.

While lethal to Lepidopteran pests, studies have shown that DiPel does not harm humans, the environment or beneficial insects that are pests’ natural predators.

CaterpillarpestingestsBtproduct(toxinsandspores),andthepeststopsfeeding within several minutes. The crop is protected.

1. The Bt toxin crystals dissolve in the midgut.

2. The Bt toxins are activated and attach to midgut receptors.

3. Pores form in the cell wall and cells rupture.

4. Bt spores will invade the insect body, death follows.

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Formulations:DiPel is available in two convenient formulations: DiPel 2X DF and DiPel WP.

Registered Crops and Rates for DiPel 2X DF:Apples: 1125 –1675g/ha(455–678g/ac)

Blueberry (Lowbush): 550–1125g/ha(223–455g/ac)

Blueberry(Highbush): 1680g/ha(680g/ac)

Broccoli: 55 –550g/ha(22–223g/ac)

Cabbage: 55 –550g/ha(22–223g/ac)

Cauliflower: 55 –550g/ha(22–223g/ac)

ChineseCabbage: 55 –550g/ha(22–223g/ac)

BokChoy: 55 –550g/ha(22–223g/ac)

ChineseBroccoli: 55 –550g/ha(22–223g/ac)

AsianRadish: 55 –550g/ha(22–223g/ac)

Chokeberry: 635g/ha(257g/ac)

Collards: 275 –550g/ha(111–223g/ac)

Cranberry: 275 –550g/ha(111–223g/ac)

Ginseng: 565 –1125g/ha(229–455g/ac)

Grape: 1125g/ha(455g/ac)

HerbsandSpices: 275 –550(111–223g/ac)

Kale: 275–550g/ha(111–223g/ac)

Lettuce: 275–550g/ha(111–223g/ac)

MustardGreens: 275–550g/ha(111–223g/ac)

Nutcrops,Parsley: 1125 –1675g/ha(455–678g/ac)

Pears: 1125 –1675g/ha(455–678g/ac)

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Potatoes: 560–1120g/ha(227–453g/ac)

Raspberry: 550–1125g/ha(223–455g/ac)

Spinach: 275 – 550g/ha(111–223g/ac)

StoneFruit(Apricot, Cherry,Plum): 1125 –1675g/ha(455–223g/ac)

SweetandFieldCorn: 560–1120g/ha(227–453g/ac)

Timothy: 140–275g/ha(57–111g/ac)

TurnipGreens: 275 –550g/ha(111–223g/ac)

Tobacco: 55 –140g/ha(23–57g/ac)

Tomato: 140–550g/ha(57–223g/ac)

Berries and small Fruit (CropGroupC13–07): 225 –1125g/ha(91–455g/ac)

Sunflower: 315 –625g/ha(127–253g/ac)

GreenhouseOrnamentals: 625g/1000L

GreenhouseHerbs: 625g/1000L

Greenhouse Vegetables (pepper,tomato,cucumber): 625g/1000L

OrnamentalandShadetrees: 65 –250g/400L

SeaBuckthorn: 125 –250g/400L

Registered Pests DiPel 2X DF:Leafrollers(Fruitree,European,Oblique–banded,Three–lined),Wintermoth,Blueberryspanworm,Chainspottedgeometer,Rannochlooper,Cabbagelooper, Diamondback moth, Imported cabbageworm, Fall Webworm, Green and Brownspanworm,GrapeBerrymoth,CranberryFruitworm,CherryFruitworm,Sunflowermoth,EuropeanCornBorer,Essex(European)skipper,Hornworm,Duponcheliafoveali,Tentcaterpillar,Elmspanworm,Fallwebworms,Spring andFallcankerworm,Bagworm,Gypsymoth,Sprucebudworm.

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Registered Crops and Rates for DiPel WP:Apples: 225 –3350g/ha(910–1356g/ac)

Broccoli: 110–1100g/ha(45–445g/ac)

Cauliflower: 110–1100g/ha(45–445g/ac)

Collards: 550–1100g/ha(45–445g/ac)

Cranberries: 550–1100g/ha(223–445g/ac)

Kale: 550–1100g/ha(223–445g/ac)

Lettuce: 550–1100g/ha(223–445g/ac)

Mustard Greens: 550–1100g/ha(223–445g/ac)

Parsley: 550g/ha(223g/ac)

Pears: 2250 – 3350g/ha(910–1356g/ac)

Raspberry: 1100–2250g/ha(445–910g/ac)

Spinach: 550–1100g/ha(223–445g/ac)

StoneFruit(Peachy,Cherry, Plum, Apricot): 2250–3350g/ha(910–1356g/ac)

Sunflower: 625–1120g/ha(253–453g/ac)

Timothy: 275–550g/ha(111–223g/ac)

Turnip Green: 550–1100g/ha(223–445g/ac)

Tobacco: 110–275g/ha(45–223g/ac)

Tomatoes: 275–1100g/ha(223–445g/ac)

OrnamentalandShadeTrees: 250–500g/400L

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Registered Pests DiPel WP:Leafrollers(Fruitree,European,Oblique-banded,Three-lined),Wintermoth,Cabbagelooper.Diamondbackmoth,Importedcabbageworm,Fruitworm,Sunflowermoth,Essex(European)skipper,Hornworm,Tomatofruitworm,Tomatohornworm,Tentcaterpillar,Elmspanworm,Fallwebworms,SpringandFall cankerworm, Bagworm, Gypsy moth.

Application Rate and Timing:Apply DiPel when insects pests are small (early instars) before crop damage has occurred. DiPel should be applied to actively growing larvae. The pest will stop feedingimmediatelyanddiewithin1-3days.Repeatatanintervalsufficienttomaintaincontrol,usually3-14daysdependingonplantgrowthrateand population density, rainfall after treating and other factors.

The best application timing is at egg hatch of the target pests, from petal fall through to green fruit stage. Weekly applications may be necessary if egg hatch is asynchronous. Also, DiPel application is similar to all common insecticides: best applied in the evening or in overcast conditions.

Good coverage is essential to ensure the crop is well protected. Apply in sufficient water volume to cover all foliage and fruit surfaces. A spreader-stick which has been approved for use on growing and harvesting crops should be added to “hard to wet” crops such as cabbage, or to improve weather fastness of the spray deposits.

The best time to apply is when the larvae are young. After eating a lethal dose of DiPel, the pest will stop feeding immediately and die within 1-3 days. Mortality varies with with larval size, lepidopteran species and dose consumed. In heavy pest pressure, higher rates of DiPel are recommended.

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The Program:Early season: Apply DiPel alone at bloom or add it to a tank-mix to improve insect control . You can also use DiPel as an economical alternative to other soft insecticides to preserve beneficial insects and to avoid flaring secondary insects.

Mid-Season:Maximizeduringthemiddle.UseDiPelmid-seasontoimproveperformance and reduce the risk of resistance due to DiPel’s multiple modes of actions.

Late Season:Finishwithflexibility.UseDiPellateseasonandenjoytheshortest re-entry and pre-harvest intervals allowed by law. With no re-entry intervals restrictions and shortest pre-harvest intervals allowed by law, DiPel can be used late season.

DiPel treated cabbage 14 days after application.

Untreated cabbage.

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Resistance Management:DiPel2XDFhasauniquemodeofaction,notrelatedtochemicalinsecticides.

DiPel2XDFisanIRACclass11insecticide,nocrossresistancehaseverbeenobserved with synthetic insecticides .

DiPel 2X DF contains four separate insecticidal Bt toxins making it difficult for resistance to develop.

Quality:DiPel is produced using the latest technology available. From the fermentor to thefield,DiPelproductionisoverseenbyusingstrictqualitystandards.Everylotof DiPel has been tested in the lab on thousands of insects before commercial releaseintothemarket.Growersbenefitfromthesamequalitycontroland superior manufacturing expertise that goes into human health products.

EachgramofDiPeloffersconsistentqualitywhichtranslatestoconsistentfield performance – leading to profitable pest control in crop production.

Untreated cabbage.11

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Quality in Formulation: DiPel provides excellent wetting properties and easy suspension. (2X DF formulation pictured.)

Mixing Instructions:Partially fill spray tank with water and provide moderate agitation. Add the requiredamountofDiPelBiologicalInsecticidewhilemaintainingagitation.Addremaining water, if any. Maintain moderate agitation while loading and spraying. Do not mix more DiPel Biological Insecticide than can be used in a 12-hour period.

Compatibility:DiPel can be tank-mixed with most other other insecticides and fungicides aswellasPlantGrowthRegulators.

Storage:In order to ensure microbial purity and potency DiPel Biological Insecticide should bestoredintheoriginalcontainerat0to25°Candusewithin24monthsofthedate of manufacture. With no re-entry intervals restrictions and shortest pre-harvest intervals allowed by law, DiPel can be used late season.

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Quality in Formulation: DiPel provides excellent wetting properties and easy suspension. (2X DF formulation pictured.)

Comparing DiPel to Conventional Insecticides:

Resistance

Mode of Action

Mode of Action

Feeding cessation

Residual,MRLlimitations

Safetytootherarthropods

Restrictions

Organicstatus

LabeledCrops

DiPel

Unique,nocross-resistancewith any chemical insecticide.

Uniquemixtureoftoxins.

Disruption of gut cells.

Fast, measured in minutes.

None,PHIeither 0orminimal.

Safeforbeneficial predators, parasites and bees.

Few restrictions on timing or location.

Yes,OMRI,NOP,othersinEurope, Asia and Latin America.

Globallyover300.

ConventionalInsecticidesPossible cross-resistance.

Singleactive.

Varied mode of action.

Good-Excellent.

PHIenforcedduetoMRL.

Oftentoxicto beneficial insects.

Generally, some toxicity to non-target organisms.

Rare.

Variable.

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Why Use DiPel?• Toreducechemicalinsecticideresidueonfoodcrops.

• Duetolimitsonamountsandfrequencyofchemicalinsecticideuse.

• Noworkerre-entryintervalrestriction.

• Forchemicalinsecticideresistancemanagement(IPM).

• Toprovidethebestpossiblecontrolconditionsforbeneficialinsects such as honey bees.

• As“activator”toenhanceefficacyofchemicalinsecticide.

• Asoneofthefewinsecticidesapprovedfororganicuse(OMRIlisted).

• MRLforexporttolerance.

• Forenvironmentalreasons.

• ReturnonInvestment(ROI).

DiPelOBLRControlinGalaApples14daysafterapplication.

Mattawa,WA.Applicationdates4/24,5/2.Airblastsprayer100gal/Ac.

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Notes:

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Nufarm is the marketer of Valent products in Canada. To learn more about DiPel® or any

other Nufarm products visit our website or call our technical support line, toll-free.

www.nufarm.ca1-800-868-5444

Thisinformationisformarketingpurposesonly.ALWAYSREADANDFOLLOWTHELABELBEFOREUSINGANYNUFARMPRODUCTS.DiPel®isaregisteredtrademarkofValentBioSciencesCorporation.

January2013

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